Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,032,423	G→A	V219V (GTC→GTT)	yedJ ←	putative HD superfamily phosphohydrolase YedJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,032,423	0	G	A	100.0%	69.3 / NA	21	V219V (GTC→GTT)	yedJ	putative HD superfamily phosphohydrolase YedJ
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (7/14);  total (7/14)

GCGCTCCAGTGATTCTCACCTACACCATTAAGCTGCGCCACCAACGTTTTAACATCATAAATTTGCAGCAGTTTCTCCAGCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTT‑ACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCA‑CGCGCTCAGCCAGCGGATGAAAACGCATCTAT‑CACCTTGTGATCGACACCTTCATTCTCCCCCGCCAGTTCGGCACTGAGCTTCGCCATAAACTCGACTAAAAAATGCGCGTTGTGCTGCGCCAGCTGCTTGCCTCTTGCGGTTTGCATGGTTTGCGGTAGCTTCAGCAACTTAGTCTGGAAATGATCCAGCGCGTAGCGTTTATCATCAAGCGGGCGATGCTGTGCGAACGGATCTTCACCATCA  >  NC_000913/2032178‑2032624                                                                                                                                                                                                                                                         |                                                                                                                                                                                                          gcgcTTCAGTGAATCTCACCGACACCATTAAGCTGCGCCACCAACTTTGTAACATCATAAATTTGCAGCAGTTTCTCCAGCATCTCCTGTGCGGCATTCCCGGTGCTGTATGAATCTGTT‑ACTGATATATTTTCCGGCATATATGTCACCGTCCATCTTAGATGGCCGACAGGTTAACATAAGTACAGCCATTTAGGGCCA‑CTCTCTCCGCCAGCGGATGAAAACGTATCGAT‑CACCGTTTGATCAacact                                                                                                                                                                                                      <  2:301896/251‑2 (MQ=255)           gATTCTCACCTACACCATGCAGCTGCAACACCAACGTTTTAACATCATAAATTTGCAGCAGTTTCTCCAGCATTGCCTGTGCGGCATTCCCGGTTCTGGATGAATCGGTT‑ACTGATATATTTTCCGGCATAGATTTCACCGTCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCA‑CGCGCTCAGCCAGCGGATGAAAACGCATCTAG‑CACCTTGTGATCAACACCTTCATTCTcc                                                                                                                                                                                            <  2:349132/251‑1 (MQ=255)                 cACCTACACCATTAAGCTGCGCCACCAACGTTTTAACATCATAAATTTGCAGCAGTTTCTCCAGCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTT‑ACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCA‑CGCGCTCAGCCAGCGGATGAAAACGCATCTAT‑CACCTTGTGATCAACACCTTCATTCTCCCCCGcc                                                                                                                                                                                      >  1:79331/1‑251 (MQ=255)                    cTACACCATTAAGCTGCGCCACCAACGTTTTAACATCATAAATTTGCAGCAGTTTCTCCAGCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTT‑ACTGATATCTTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCA‑CGCGCTCAGCCAGCGGATGAAAACGCATCTAT‑CACCTTGTGATCAACACCTTCATTCTCCCCCGCCAGt                                                                                                                                                                                   >  1:144879/1‑251 (MQ=255)                                gCTGCGCCCCCAACGTTTTAACATCATAAATTTGCAGCAGTTTCTCCAGCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTT‑ACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCA‑CGCGCTCAGCCAGCGGATGAAAACGCATCTAT‑CACCTTGTGATCAACACCTTCATTCTCCCCCGCCAGTTCGGCACTGAGc                                                                                                                                                                       <  1:252312/251‑1 (MQ=255)                                             cGTTTTAACATCATAAATTTGCAGCAGTTTCTCCAGCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTT‑ACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCA‑CGCGCTCAGCCAGCGGATGAAAACGCAGCTAT‑CACCTTGTGATCAACACCTTCATTCTCCCCCGCCAGTTCGGCACTGAGCTTCGCCATAAACt                                                                                                                                                          <  1:163093/251‑1 (MQ=255)                                                  tAACATCATAAATGTGAACTAGTTTCTCCAGCGTGGGCTGTGCGGCATTCCCGGTGCTGTATGAAT‑GGTTGACGGCTATATTTTCCTGCATAGATTTCCCCGGCCATCTGAGATGGCCGACAGTTTAACACAATTACATCCATTTAGGTCAAACG‑GCGCAGCCAGCGGATGAAAACTTTTCGATTGACGGT‑TGACCAACACCTTCGGTCCCCCCCGCCAGTTCGGCACTGAGCGGCGCCATAAATTCGAc                                                                                                                                                      <  2:172541/250‑1 (MQ=255)                                                      atcatAAATTTGCAGCAGTTTCTCCAGCATTGCCTGTGCGGCAGTCCCGGTGCTGTATGAATCGGTT‑ACATATATATTTTCCTGCATAGGTTTCACCGGCCATCGGGGATGGCAGCCAGGTTAACATAATTACAGCCAATTAGGGCCA‑CGCGCTCAGCCAGCGGATGAAAACGCAGCTAT‑CCCCTTGTTATCAACACCTTCATTCTCCCCCGCCAGTTCGGCACTGAGCTTCGCCATAAACACGACTaaaa                                                                                                                                                 <  2:343465/251‑1 (MQ=255)                                                          taattttGCAGCAGTTGCCACAACATTGCCTGTGCGGCTTTCCTGGTGCTGTATGAATAGGTT‑TCTAATATATTTTCCGGCATAGATTTCACCGGCCATCTGTGATGGCCGCCAGGTTAACATAATTACAGCCATTTAGGGCCA‑CGCGCTCAGACAGCGGATGAAAACGCTTCTAT‑CACCTTGTGATCAACACCCTCAATCACCACCGCCAGTTCGGCACTGAGCTTCGCCATAAACTCGACTaaaaa                                                                                                                                                <  2:232258/244‑1 (MQ=255)                                                          tAAATTTGCAGCAGTTTCTCCAGCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTT‑ACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCA‑CGCGCTCAGCCAGCGGATGAAAACGCATCTAT‑CACCTTGTGATCAACACCTTCATTCTCCCCCGCCAGTTCGGCACTGAGCTTCGCCATGAACTCGACTAAAAAATg                                                                                                                                             >  2:104725/1‑251 (MQ=255)                                                           aaaTTTGCAGCAGTTTCTCCAGCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTT‑ACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACAAAATTACAGCCATTTAGGGCCA‑CGCGCTCAGCCAGCGGATGAAAACGCATCTAT‑CACCTTGTGATCAACACCTCCATTCTCCCCCGCCAGTTCGGCACTGAGCTTCGCCATAAACTCGACTAAAAAATgc                                                                                                                                            <  2:38771/251‑1 (MQ=255)                                                            aaTTTGCAGCAGTTTCTCCAGCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTT‑ACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCAA‑CGCGCTCAGCCAGCGGATGAAAACGCCTCTAT‑CACCTTGTGATCAACACCTTCATTCTCCCCCGCCAGTTCGGCACTGAGCTTCGCCATAAACTCGACTAAAAAATgcg                                                                                                                                           >  2:291134/1‑251 (MQ=255)                                                                                                          cTGTATGAATCGGTT‑ACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCA‑CGCGCTCAGCCAGCGGATGAAAACGCATCTAT‑CACCTTGTGATCAACCCCTTCATTCTCCCCCGCCAGTTCg                                                                                                                                                                                <  2:260862/168‑1 (MQ=255)                                                                                                          cTGTATGAATCGGTT‑ACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCA‑CGCGCTCAGCCAGCGGATGAAAACGCATCTAT‑CACCTTGTGATCAACACCTTCATTCTCCCCCGCCAGTTCg                                                                                                                                                                                >  1:260862/1‑168 (MQ=255)                                                                                                                             gATATATTTTCCTGCATAGATTTCCCCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCA‑CGCGCTCAGCCAGCGGATGAAAACGCATCTAT‑CACCTTGTGATCAACACCTTCATTCTCCCCCGCCAGTTCGGCACTGAGCTTCGCCATAAACTCGACTAAAAAATGCGCGTTGTGCTGCGCCAGCTGCTTGCCTCTTGCGGTTTGCATGGTTTGCGGTAGCTTCAGCAACtt                                                                           <  1:96077/251‑1 (MQ=255)                                                                                                                                          gCATAGATTTCCCCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCA‑CGCCCTCAGCCAGCTGATGAAAACGCATCTAT‑CACCTTGTGATCAACCCCTTCATTCTCCCCCGCCAGTTCGGCACTGAGCTTCGCCATAAACTCGACTAAAAAATGCGCGTTGTGCTGCGCCAGCTGCTTGCCTCTTGCGGTTTGCATGGTTTGCGGTAGCTTCAGCAACTTAGTCTGGAAAt                                                                <  1:210856/249‑1 (MQ=255)                                                                                                                                              aGATTTCACCGGCCAGCTGGGATGGCCGACAGGTTAACATAATAACAGCAATTTAGGGCCA‑CGCGCTCAGCCAGCGGATGAAAACGCAGCTAT‑CCCCGTGTTATCAACACCTTCATTCTCCCCCGCCAGGTCGGCACTGAGCTTCGCCATAAACTCGACTAAAAAATGCGCGTTGTGCTGCGCCAGCTGCTTGCCTTTTGCGGGTTGCATGGGTTGCGGTAGCTTCAGCAACTGAGTCTGGAAATGATCCa                                                          <  2:55145/251‑1 (MQ=255)                                                                                                                                                    cACCGGCCATCTGAGATGGCCGACAGGTTAACATCATTACAGCCATTTAGGGCCA‑CGCGCTCAGCCAGCGGATGAAAACGCATCTAT‑CACCTTGTGATCAACACCTTCATTCTCCCCCGCCAGTTCGGCACTGAGCTTCGCCATAAACTCGACTAAAAAATGCGCGTTGTGCTGCGCCAGCTGCTTGCCTCTTGCGGTTTGCATGGTTTGCGGTAGCTTCAGCAACTTAGTCTGGAAATGATCCAGCGCGt                                                    >  2:67803/1‑251 (MQ=255)                                                                                                                                                     aCCGGCCATCTGAGAGGGCCGACAGGTTAAAATAATTACAGCCATTTAGGGCCA‑CGCGCTCAGCCAGCGGATGAAAACGCATCTAT‑CACCTTGTGACCAACACCTTCATTCTCCCCCGCCAGTTCGGCACTGAGCTTCGCCATAAACTCGACTAAAAAATGCGCGTTGTGCTGCGCCAGCTGCTTGCCTCTTGCGGTTTGCATGGTTTGCGGTAGCTTCAGCAACTTAGTCTGGAAATGATCCAGCGCGTa                                                   <  2:79331/251‑1 (MQ=255)                                                                                                                                                                           cAGGTTAACATAATTACAGCCATTTAGGGCCA‑CGCGCTCAGCCAGCGGATGAAAACGCATCTAT‑CACCTTGTGATCAACACCTTCATTCTCCCCCGCCAGTTCGGCACTGAGCTTCGCCATAAACTCGACTAAAAAATGCGCGTTGTGCTGCGCCAGCTGCTTGCCTCTTGCGGTTTGCATGGTTTGCGGTAGCTTCAGCAACTTAGTCTGGAAATGATCCAGCGCGTAGCGTTTATCATCAAGCGGGCGa                             <  2:306818/251‑1 (MQ=255)                                                                                                                                                                                                      ggCCA‑CGCGCTCAGCCAGCGGATGAAAACGCATCTAT‑CACCTAGTGATCAACACCTTCATTCTCCCCCGCCAGTTCGGCACTGAGCTTAGCCATAAACTCGACTAAAAAATGCGCGTTGTGCTGCGCCAGCTGCTTGCCTCTTGCGGTTTGCATGGTTTGCGGTAGCTTCAGCAACTTAGTCTGGAAATGATCCAGCGCGTAGCGTTTATCATCAAGCGGGCGATGCTGTGCGAACGGATCTTCACCATcc  >  2:213432/1‑250 (MQ=255)                                                                                                                                                                                                                                                         |                                                                                                                                                                                                          GCGCTCCAGTGATTCTCACCTACACCATTAAGCTGCGCCACCAACGTTTTAACATCATAAATTTGCAGCAGTTTCTCCAGCATTGCCTGTGCGGCATTCCCGGTGCTGTATGAATCGGTT‑ACTGATATATTTTCCTGCATAGATTTCACCGGCCATCTGAGATGGCCGACAGGTTAACATAATTACAGCCATTTAGGGCCA‑CGCGCTCAGCCAGCGGATGAAAACGCATCTAT‑CACCTTGTGATCGACACCTTCATTCTCCCCCGCCAGTTCGGCACTGAGCTTCGCCATAAACTCGACTAAAAAATGCGCGTTGTGCTGCGCCAGCTGCTTGCCTCTTGCGGTTTGCATGGTTTGCGGTAGCTTCAGCAACTTAGTCTGGAAATGATCCAGCGCGTAGCGTTTATCATCAAGCGGGCGATGCTGTGCGAACGGATCTTCACCATCA  >  NC_000913/2032178‑2032624

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑