Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,507,867	G→A	E363K (GAG→AAG)	rlhA →	23S rRNA 5‑hydroxycytidine C2501 synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,507,867	0	G	A	100.0%	93.3 / NA	28	E363K (GAG→AAG)	rlhA	23S rRNA 5‑hydroxycytidine C2501 synthase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (14/14);  total (14/14)

GAACATTTCTTTGTTCCATCGACGGAAAAGACTTTCCACCGTGGTAGCACAGATTATTTTGTGAATGCCCGTAAAGGCGATATTGGCGCGTTCGATTCGCCGAAATTTATCGGCCTGCCGGTAGGCGAAGTAGTGAAAGTGGCGAAAGATCATCTCGATGTTGCCGTTACCGAGCCACTGGCAAATGGCGATGGCCTGAACGTGTTGATTAAACGTGAAGTCGTCGGTTTTCGTGCCAATACGGTCGAGAAAACCGGAGAAAATCAGTACCGCGTCTGGCCCAATGAAATGCCAGCAGATTTGCACAAAATTCGTCCACATCACCCACTAAACCGTAATCTTGATCATAACTGGCAGCAGGCACTGACAAAAACCTCCAGCGAACGTCGGGTGGCGGTAGACATTGAACTGGGCGGCTGGCAGGAACAACTGATTCTGACCCTCACCAGTGAAGAGGGTGTCAGCATCACGCATACGCT  >  NC_000913/1507621‑1508099                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                         gAACATTTCTTTTTTCCATCGACGGAAAAGACTTTCCACCGTGGTAGCCCAGATTATTTTGTGAATGCCCGTAAAGGCGATATTGGCGCGTTCGATTCGCCGAAATTTATCGGCCTGCCGGTAGGCGAAGTAGTGAAAGTGGCGAAAGATCATCTCGATGTTGCCGTTACCGAGCCACTGGCAAATGGCGATGGCCTGAACGTGTTGATTAAACGTGAAGTCGTCGGTTTTCGTGCCAATACGGTCaaga                                                                                                                                                                                                                                       <  2:24133/250‑1 (MQ=255)                ccATCGACGGAAAAGACTTTCCACCGTGGTAGCACAGATTATTTTGTGAATGCCCGTAAAGGCGATATTGGCGCGTTCGATTCGCCGAAATTTATCGGCCTGCCGGTAGGCGAAGTAGTGAAAGTGGCGAAAGATCATCTCGATGTTGCCGTTACCGAGCCACTGGCAAATGGCGATGGCCTGAACGTGTTGATTAAACGTGAAGTCGTCGGTTTTCGTGCCAATACGGTCAAGAAAACCGGAGAAAATc                                                                                                                                                                                                                        >  1:202551/1‑250 (MQ=255)                       cGGAAAAGACTTTCCACCGTGGTAGCACAGATTATTTTGTGAATGCCCGTAAAGGCGATATTGGCGCGTTCGATTCGCCGAAATTTATCGGCCTGCCGGTAGGCGAAGTAGTGAAAGTGGCGAAAGATCATCTCGATGTTGCCGTTACCGAGCCACTGGCAAATGGCGATGGCCTGAACGTGTTGATTAAACGTGAAGTCGGAGGTTTGCGTGCCAATACGGTCAAGAAAACCGGAGAAAATCCGTACcgc                                                                                                                                                                                                                >  1:193620/1‑251 (MQ=255)                           aaaGCCTTTCCGCCGTGGTATCAAATATTTTTTTGTGAATGCCCGTAAGGGCGATATTGGCGCTTTCGATTCGCCGAAATTTTTCGGCCTGCCGGTAGGCGAAGTAGTGAAAGTGGCGAAAGATCATCTCGATGTTGCCGTTACCGAGCCACTGGCAAATGGCGATGGCCTGAACGTGTTGATTAAACGTGAAGTCGTCGGTTTTCGTGCCAATACGGTCAAGAAAACCGGAGAAAATCAGTACCGCGTCt                                                                                                                                                                                                            <  2:18973/251‑1 (MQ=255)                              gACTTTCCACCGTGGTAGCACAGATTATTTTGTGAATGCCCGTAAAGGCGATATTGGCGCGTTCGATTCGCCGAAATTTATCGGCCTGCCGGTAGGCGAAGTAGTGAAAGTGGCGAAAGATCATCTCGGTGTTGCCGTTACCGAGCCAATGGAAAATGGCGATGGCTTGAACGTGTTGATTAAACGTGAAGTCGTCGGTTTTCGTGCCAATACGGTCAAGAAAACCGGAGAAAATCAGAACCGAGTCTGGc                                                                                                                                                                                                         >  1:123926/1‑251 (MQ=255)                                     cACCGTGGTAGCACAGATTATTTTGTGAATGCCCGTAAAGGCGATATTGGCGCGTTCGATTCGCCGAAATTTATCGGCCTGCCGGTAGGCGAAGTAGTGAAAGTGGCGAAAGATCATCTCGATGTTGCCGTTACCGAGCCACTGGCAAATGGCGATGGCCTGAACGTGTTGATTAAACGTGAAGTCGTCGGTTTTCGTGCCAATACGGTCAAGAAAACCGGAGAAAATCAGTACCGCGTCTGGCCCAATGa                                                                                                                                                                                                  <  2:39681/251‑1 (MQ=255)                                       ccGTGGTAGCACAGATTATTTTGTGAATGCCCGTAAAGGCGATATTGGCGCGTTCGATTCGCCGAAATTTATCGGCCTGCCGGTAGGCGAAGTAGTGAAAGTGGCGAAAGATCATCTCGATGTTGCCGTTACCGAGCCACTGGCAAATGGCGATGGCCTGAACGTGTTGATTAAACGTGAAGTCGTCGGTTTTCGTGCCAATACGGTCAAGAAAACCGGAGAAAATCAGTACCGCGTCTGGCCCAATGaaa                                                                                                                                                                                                <  1:338517/251‑1 (MQ=255)                                                 acaGATTATTTTGTGAATGCCCGTAAAGGCGATATTGGCGCGTTCGATTCGCCGAAATTAATCGGACTGCCGGTAGGCGAAGTAGTGAAAGTGGCGAAAGATCATCTCGATGTTGCCGTTACCGAGCCACTGGCAAATGGCGATGGCCTGAACGTGTTGATTAAACGTGAAGTCGTCGGTTTTCGTGCCAATACGGTCAAGAAAACCGGAGAAAATCAGTACCGCGTCTGGCCCAATGAAATGCcagc                                                                                                                                                                                         >  1:25413/1‑248 (MQ=255)                                                 acaGATTATTTTGTGAATGCCCGTAAAGGCGATATTGGCGCGTTCGATACGCCGAAATTTATCGGCCTGCCGGTAGGCGAAGTAGTGAAAGTGGCGAAAGATCATCTCGATGTTGCCGTTACCGAGCCACTGGCAAATGGCGATGGCCTGAACGTGTTGATTAAACGTGAAGTCGTCGGTTTTCGTGCCAATACGGTCAAGAAAACCGGAGAAAATCAGTACCGCGTCTGGCCCAATGACATGCcagc                                                                                                                                                                                         >  1:137284/1‑248 (MQ=255)                                                              tgAATGCCCGTAAAGGCGATATTGGCGCGTTCGATTCGCCGAAATTTATCGGCCTGCCGGGAGGCGAAGTAGTGAAAGTGGCGAAAGATCATCTCGATGTTGCCGTTACCGAGCCACTGGCAAATGGCGATGGCCTGAACGTGTTGATTAAACGTGAAGTCGTCGGTTTTCGTGCCAATACGGTCAAGAAAACCGGAGAAAATCAGTACCGCGTCGGGCCCAATGAAATGCCAGCAATTTTGCACAAAAtt                                                                                                                                                                         >  1:276518/1‑251 (MQ=255)                                                                    cccGTAAAGGCGATATTGGCGCGTTCGATTCGCCGAAATTTATCGGCCTGCCGGTAGGCGGAGTAGTGAAAGTGGCGAAAGATCATCTCGATGTTGCCGTTACCGAGCCACTGGCAAATGGCGATGGCCTGAACGTGTTGATTCAACGTGAAGTCGTCGGTTTTCGTGCCAATACGGTTAAGAAAACCGGAGAAAATCAGTACCGCGTCTGGCCCAATGAAATGCCAGCAGATTTGCACAAAATTCGTcc                                                                                                                                                                    >  2:216516/1‑250 (MQ=255)                                                                                 tatTGGCGCGTTCGATTCGCCGAAATTTATCGGCCTGCCGGTAGGCGAAGTAGTGAAAGTGGCGAAAGATCATCTCGATGTTGCCGTTACCGAGCCACTGGCAAATGGCGATGGCCTGAACGTGTTGATTAAACGTGAAGTCGTCGGTTTTCGTGCCAATACGGTCAAGAAAACCGGGGAAAATCAGTACCGCGTCTGGCCCAATGAAATGCCAGCAGATTTGCACAAAATTCGTCCACATCACCCACTa                                                                                                                                                       >  2:101769/1‑250 (MQ=255)                                                                                    tGGCGCGTTGGATTCGCGGAAATTTATCGGCCTGCCGGTAGGCTAAGTAGTGAAAGTGGCGAAAGATCATCGCGATGTTGCCGTTACCGAGCCACTGGCAAATGGCGATGGCCTGAACGTGTTGATTAAACGTGAAGTCGTCGGTTTTCGTGCCAATACGGTCAAGAAAACCGGAGAAAATCAGTACCGCGTCTGGCCCAATGAAATGCCAGCAGATTTGCACAAAATTCGTCCACATCACCCGCTAAAc                                                                                                                                                    <  2:59968/250‑1 (MQ=255)                                                                                         cgTTCGATTCGCCGAAATTTATCGGCCTGCCGGTAGGCGAAGTAGTGAAAGTGGCGAAAGATCATCTCGATGTTGCCGTTACCGAGCCACTGGCAAATGGCGATGGCCTGAACGAGTTGATTAAACGTGAAGTCGTCGGTTTTCGTGCCAATACGGTCAAGAAAACCGGAGAAAATCAGTACCGCGTCTGGCCCAATGAAATGCCAGCAGATTTGCACAAAATTCGTCCACATCACCCACTAAACCGTAAt                                                                                                                                              >  2:330999/1‑251 (MQ=255)                                                                                                               cGGCCTGCCGGTAGGCGAAGTAGTGAAAGTGGCGAAAGATCATCTCGATGTTGCCTTTACCGAGCCACTGGCAAATGGCGATGGCCTGAACGTGTTGATTAAACGTGAAGTCGTCGGTTCTCGTGCCAATACGGTCAAGAAAACCGGAGAAAATCAGTACCGCGTCTGGCCCAATGAAATGCCAGCAGATTTGCACAAAATTCGTCCACATCACCCACTAAACCGTAAGCTTGATCATAACTTGCAGCAgg                                                                                                                        <  1:108727/251‑1 (MQ=255)                                                                                                                                                              aTGTTGCTGTTACCGAGCAACTGGCAAGTGGCGATGGCCTGAACGTGTTGATTAAACGTGAAGTCGTCGCGTTTCGTGCCAATACGGTCAGGAAAACAGGAGAAAATCAGTACCGCGTCTGGCCCAATGAAATGCCAGCAGATTTGCACAAAATTCGTCCACATCGCCCACTAAACCGTAATCTTGATCATAACTGGCAGCAGGCACTGACAACAACCTCCTGCGAACGTAGGGTGGCGGTAGACATTGa                                                                          >  2:14587/1‑250 (MQ=255)                                                                                                                                                               tGTTGCCGTTACCGAGCCACTGGCAAATGGCGATGGCCTGAACGTGTTGATTAAACGTGAAGTCGTCGGTTTTCGTGCCAATACGGTCAAGAAAACCGGAGAAAATCAGTACCGCGTCTGGCCCAATGAAATGCCAGCAGATTTGCACAAAATTCGTCCACATCACCCACTAAACCGTAATCTTGATCATAACTAGCAGCAGGCACTGACAAAAACCTCCAGCGAACGTCGGGTGGCGGTAGACATTGaa                                                                         >  1:133394/1‑250 (MQ=255)                                                                                                                                                                gTTGCCGTTACCGAGCCACTGGCAAATGGCGATGGCCTGAACGTGTTGATTAAACGTGAAGTCGTCGGTTTTCGTGCCAATACGGTCAAGAAAACCGGAGAAAATCAGTACCGCGTCTGGCCCAATGAAATGCCAGCAGATTTGCACAAAATTCGTCCACATCACCCACTAAACCGTAATCTTGATCATAACTGGCAGCAGGCACTGACAAAAACCTCCAGCGAACGTCGGGTGGCGGTAGACATTGAACt                                                                       >  1:180085/1‑251 (MQ=255)                                                                                                                                                                  tGCCGTTACCGAGCCACTGGCAAATGGCGATGGCCTGAACGTGTTGATTAAACGTGAAGTCATCGGTTTTCGTGCCAATACGGTCAAGAAAACCGGAGAAAATCAGTACCGCGTCTGGCCCAATGAAATGCCAGCAGATTTGCACAAAATTCGTCCACATCACCCACTAAACCGTAATCTTGATCATAACTGGCAGCAGGCACTGACAAAAACCTCCAGCGAACGTCGGGTGGCGGTAGACATTGAACTg                                                                      <  1:345230/250‑1 (MQ=255)                                                                                                                                                                             aGCCACTGGCAAATGGCGATGGCCTGAACGTGTTGATTAAACGTGAAGTCGTCGGTTTTCGTGCCAATACGGTCAAGAAAACCGGAGAAAATCAGTACCGCGTCTGGCCCAATGAAATGCCAGCAGATTTGCACAAAATTCGTCCACATCACCCACTAAACCGTAAGCT‑GATCATAACTGGCAGCAGGCACTGACAAAAACCTCCAGCGAACGTCGGGTGGCGGTAGACATTGAACTGGGCGGCTGGCAgg                                                         <  2:202551/251‑1 (MQ=255)                                                                                                                                                                               ccACTGGCACATGGCGATGGCCTGAACGTGTTGATTAAACGTGAAGTCGTCGGTTTTCGTGCCAATACGGTCAAGAAAACCGGAGAAAATCAGTACCGCGTCTGGCCCAATGAAATGCCAGCAGATTTGCACAAAATTCGTCCACATCAccc                                                                                                                                                           >  2:116846/1‑152 (MQ=255)                                                                                                                                                                               ccACTGGCAAATGGCGATGGCCTGAACGTGTTGATTAAACGTGAAGTCGTCGGTTTTCGTGCCAATACGGTCAAGAAAACCGGAGAAAATCAGTACCGCGTCTGGCCCAATGAAATGCCAGCAGATTTGCACAAAATTCGTCCACATCAccc                                                                                                                                                           <  1:116846/152‑1 (MQ=255)                                                                                                                                                                                     gCAAATGGCGATGGCCTGAACGTGTTGATTAAACGTGAAGTCGTCGGTTTTCGTGCCAATACGGTCAAGAAAACCGGAGAAAATCAGTACCGCGTCTGGCCCAATGAAATGCCAGCAGATTTGCACAAAATTCGTCCACATCACCCACTAAACCGTAATCTTGATCATAACTGGCAGCAGGCACTGACAAAAACCTCCAGCGAACGTCGGGTGGCGGTAGACATTGAACTGGGCGGCTGGCAGGAACAACt                                                  <  1:128436/251‑1 (MQ=255)                                                                                                                                                                                       aaaTGGCGATGGCCTGAACGTGTTGATTAAACGTGAAGTCGTCGGTTTTCGTGCCAATACGGTCAAGAAAACCGGAGAAAATCAGTACCGCGTCTGGCCCAATGAAATGCCAGCAGATTTGCACAAAATTCGTCCACATCACCCACTAAACCGTAATCTTGATCATAACTGGCAGCAGGCACTGACAAAAACCTCCAGCGAACGTCGGGTGGCGGTAGACATTGAACTGGGCGGCTGGCAGGAACAACTGa                                                <  1:159657/251‑1 (MQ=255)                                                                                                                                                                                                      gaacttggtGATTAAACGTGAAGTCGTCGGTTTTCGGGCCAATACGGTCAAGAAAACCGGAGAAAATCAGTACCGCGTCTGGCCCAATGAAATGCCAGCAGATTTGCACAAAATTCGTCCACATCACCCACTAAACCGTAATCTTGATCATAACTGGCAGCAGGCACTGACAAAAACCTCCAGCGAACTTCGGGTGTCGGTAGACATTGAACTGGGCGGCTGGCAGGAACAACTGATTCTGACCCTCACCa                                 <  2:137284/243‑1 (MQ=255)                                                                                                                                                                                                      gAACGTGTTGATTAAACGTGAAGTCGTCGGTTTTCGTGCCAATACGGTCAAGAAAACCGGAGAAAATCAGTACCGCGTCTGGCCCAATGAAATGCCAGCAGATTTGCACAAAATTCGTCCACATCACCCACTAAACCGTAATCTTGATCATAACTGGCAGCAGGCACTGACAAAAACCTCCATCGAACGTCGGGTGGCGGTAGACATTGAACTGGGCGGCTGGCAGGAACAACTGATTCTGACCCTCACCa                                 <  2:25413/251‑1 (MQ=255)                                                                                                                                                                                                      gAACGTGTTGATTAAACGTGAAGTCGTCGGTTATCGTGCCAATACGGTCAAGAAAACCGGAGAAAATCAGGACCGCGTCTGGCCCAATGAAATGCCAGCAGATTTGCACAAAATTCGTCCACATCACCCACTAAACCGTAATCTTGATCATAACTGGCAGCAGGCACTGACAAAAACCTCCAGCGAACGTCGGGTGGCGGTAGACATTGAACTGGGCGGCTGGCAGGAACAACTGATTCTGACCCTCAcc                                  <  1:182265/250‑1 (MQ=255)                                                                                                                                                                                                                aTTAAACGTGAAGTCGTCGGTTTTCGTGCCAATACGGTCAAGAAAACCGGAGAAAATCAGTACCGCGTCTGGCCCAATGAAATGCCAGCAGATTTGCACAAAATTCGTCCACATCACCCACTAAACCGTAATCTTGATCATAACTGGCAGCAGGCACTGACAAAAACCTCCAGCGAACGTCGGGTGGCGGTAGACATTGAACTGGGCGGCTGGCAGGAACAACTGATTCTGACCCTCACCAGTGAAGAggg                       >  1:199530/1‑251 (MQ=255)                                                                                                                                                                                                                                      ttCGTGCCAATACGGTCAAGAAAACCGGAGAAAATCAGTACCGCGTCTGGCCCAATGAAATGCCAGCAGATTTGCTCAAAATTCGTCCACATCACCCACTAAACCGTAATCTTGATCATAACGGGCATCAGGCACTGACAAAAACCTCCAGCGAACGTCGGGTGGCGGTAGACATTGAACTGGGCGGCTGGCAGGAACAACTGATTCTGACCCTCACCAGTGAAGAGGGTGCCAGCATCACGCATACGCt  <  1:236461/250‑1 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                         GAACATTTCTTTGTTCCATCGACGGAAAAGACTTTCCACCGTGGTAGCACAGATTATTTTGTGAATGCCCGTAAAGGCGATATTGGCGCGTTCGATTCGCCGAAATTTATCGGCCTGCCGGTAGGCGAAGTAGTGAAAGTGGCGAAAGATCATCTCGATGTTGCCGTTACCGAGCCACTGGCAAATGGCGATGGCCTGAACGTGTTGATTAAACGTGAAGTCGTCGGTTTTCGTGCCAATACGGTCGAGAAAACCGGAGAAAATCAGTACCGCGTCTGGCCCAATGAAATGCCAGCAGATTTGCACAAAATTCGTCCACATCACCCACTAAACCGTAATCTTGATCATAACTGGCAGCAGGCACTGACAAAAACCTCCAGCGAACGTCGGGTGGCGGTAGACATTGAACTGGGCGGCTGGCAGGAACAACTGATTCTGACCCTCACCAGTGAAGAGGGTGTCAGCATCACGCATACGCT  >  NC_000913/1507621‑1508099

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑