Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,429,236	0	T	A	52.9%	24.7 / 96.0	71	L63* (TTG→TAG)	stfR	putative prophage side tail fiber protein StfR
Reads supporting (aligned to +/- strand):  ref base T (22/11);  new base A (26/11);  total (48/23)
Fisher's exact test for biased strand distribution p-value = 8.00e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.75e-01
Rejected as consensus: Frequency below/above cutoff threshold.

TATGACAGCCCGCCGGTTCAGGCGGGCTTTTTTGTGGGGTGAATATGGCAGTAAAGATTTCAGGTGTACTGAAAGACGGCACAGGAAAACCGGTACAGAACTGCACAATCCAGCTGAAAGCAAAACGTAACAGCACCACGGTGGTGGTGAACACGCTGGCCTCAGAAAATCCGGATGAAGCCGGGCGTTACAGCATGGACGTTGAGTACGGTCAGTACAGCGTTATTCTGTTGGTGGAAGGATTCCCGCCGTCACATGCCGGGACCATTACCGTGTATGAAGATTCTCAACCCGGTACGCTGAATGATTTTCTCGGTGCCATGACGGAGGATGATGCCCGTCCGGAGGCACTGCGCCGTTTTGAACTGATGGTGGAAGAGGTGGCGCGTAACGCGTCCGCGGTGGCACAGAACACGGCAGCCGCGAAGAAGTCAGCCAGTGATGCCAGCACATCAGCCCGTGAGGCGGCAACCCATGC  >  NC_000913/1429005‑1429482                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                       gaTGACAGCCCGCCGGTTCAGGCGGGGTTTTTTGTGGGGTGAATATGGCAGTAAAGATTTCAGGTGTACTGAAAGACAGCACAGGAAAACCGGTACAGAACTGCACAATCCAGCTGAAAGCAAAACGTAACAGCACCACGGTGGTGTTGAACACTCTGGCCTCAGAAAATCCGGAGGAAGCCGGGCGTTACAGCATGGACGTTGAGTACGGTCAGTACAGCGTTATTCTGTTGGTGGAAGGATTCccgcc                                                                                                                                                                                                                                      <  1:323807/249‑1 (MQ=255)      cAGCCCGCCGGTTCAGGCGGGCTTTTTTGTGGGGTGAATATGGCAGTAAAGCTTTCAGGTGTACTGAAAGACGGCACAGGAAAACCGGTACAGAACTGCACAATCCAGCTGAAAGCAAACCGTAACAACACCCCGGTGGTGGTGAAAACGCTGGCCTCAGAAAATCCGGATGAAGCCGGGCGTTACAGCATGGACGTTGAGTACGGTCAGTACAGCGTTATTCTGTTGGTGGAAGGATTCCCGCCGTcac                                                                                                                                                                                                                                 >  1:323834/1‑250 (MQ=255)               ggTTCAGGCGGGCTTTTTTGTGGGGTGAATATGGCAGTAAAGATTTCAGGTGTACTGAAAGACGGCACAGGAAAACCGGTACAGAACTGCACAATCCAGCTGAAAGCAAAACGTAACAGCACCACGGTGGTGGTGAACACGCTGGCCTCAGAAAATCCGGATGAAGCCGGGCGTTACAGCATGGACGTTGAGTACGGTCAGTACAGCGTTATTCTGTTGGTGGAAGGATTCCCGCCGTCACATGCCGGGAc                                                                                                                                                                                                                       >  1:86022/1‑251 (MQ=255)                     ggCGGGCTTTTTTGTGGGGTGAATATGGCAGTAAAGATTGCAGGTGTACTGAAAGACGGCACAGGAAAACCGGTACATAACTGCACAATCCAGCTGAAAGCAAAACGTAACAGCACCACGGGGGTGGGGAACAGGCTGGCCTCAGAAAATCGGGATGAAGCCGGGCTTTATAGCATGGACGTTGAGTACGGTCATTACAGCGTTACTCTGTTGTTGGAAGGATTCCCGCCGTCACATGCCGGGACCATTAc                                                                                                                                                                                                                 <  1:212371/251‑1 (MQ=255)                          gCTTTTTTGTGGGGTAAATATGGCAGTAAAGATTTCAGGTTAACGGAAAGACGGCACAGGAAAACCGGTACAGAACGGCAAAATCCAGCTGAAAGCAAAACGTAACAGCCCCACGGTTGTGGTGAACACGCTGGCCTCATAAAATCCGGATGAAGCCGGGCGTTACATCATGGACGTTGAGTACGGTCAGTACAGCGTTATTCTGTTGGTGGAAGGATTCCCGCCGTCACATGCCGGGACCATTACCgtgt                                                                                                                                                                                                            <  1:24294/251‑1 (MQ=255)                                  gtgGGGTGAATATGGCAGTAAAGATTTCAGGAGTCCTGAAAGACGGCACAGGAAAACCGGTACAGAACTGCACCATTCAGCTGAAAGCCAGACGTAACAGCACCACGGTGGTGGTGAACACGGTGGGCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGAt                                                                                                                                                                                                    <  1:8352/251‑1 (MQ=255)                                    ggggTGAATATGGCAGTAAAGATTTCAGGTGTACTGAAAGACGGCACAGGAAACCCGGTACAGAACTGCACAATCCAGCTGAAAGCAAAACGTACCAGCACCACGGTGGTGGTGAACACGCTGGCCTCAGAAAATCCGGATGAAGCCGGGCGTTACAGCATGGACGTTGAGTACGGTCAGTACAGCGTTATTCTGTTGGTGGAAGGATTCCCGCCGTCACATGCCGGGACCATTACCGTGTATGAAGATtc                                                                                                                                                                                                  <  2:63714/251‑1 (MQ=255)                                       gTGAATATGGCAGTAAAGATTTCAGGAGTCCTGAAAGACGGCACAGGAAAACCGGTACAGAACTGCACCATTCAGCTGAAAGCCAGACGTAACAGCACCACGGTGGTGGTGAACACGGTGGGCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATtcac                                                                                                                                                                                                >  1:190936/1‑248 (MQ=255)                                           atatGGCAGTAAAGATTTCAGGAGTCCTGAAAGACGGCACAGGAAAACCGGTACAGAACTGCACCATTCAGCTGAAAGCCAGACGTAACAGCACCACGGTGGTGGTGCACACGGTGGGCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAAccg                                                                                                                                                                                           >  2:177041/1‑250 (MQ=255)                                             atGGCAGTAAAGATTTCAGGAGTCCTGAAAGACGGCACAGGAAAACCGGTACAGAACTGCACCATTCAGCTGAAAGCCAGACGTAACAGCACCACGGTGGTGGTGAACACGGTGGGCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAAccggg                                                                                                                                                                                         <  1:119242/251‑4 (MQ=255)                                                 cAGTAAAGATTTCAGGTGTACTGAAAGACGGCACAGGAAAACCGGTACAGAACTGCACAATCCAGCTGAAAGCAAAACGTAACAGCACCACGGTGGTGGTGAACACGCTGGCCTCAGAAAATCCGGATGAAGCCGGGCGTTACAGCATGGACGTTGAGTACGGTCAGTACAGCGTTATTCTGTTGGTGGAAGGATTCCCGCCGTCACATGCCGGGACCATTACCGTGTATGAAGATTCTCAACCCGGTACg                                                                                                                                                                                     >  2:31399/1‑251 (MQ=255)                                                   gTAAAGATTTCAGGTGTACTGAAAGACGGCACAGGAAAACCGGTACAGAACTGCACAATCCAGCTGAAAGCAAAACGTAACAGCACCACGGTGGTGGTGAACACGCTGGCCTCAGAAAATCCGGATGAAGCCGGGCGTTACAGCATGGACGTTGAGTACGGTCAGTACAGCGTTATTCTGTTGGTGGAAGGATTCCCGCCGTCACATGCCGGGACCATTACCGTGTATGAAGATTCTCAACCCGGTACGCt                                                                                                                                                                                   <  1:19093/251‑1 (MQ=255)                                                        gATTTCAGGTGTACTGAAAGACGGCACAGGAACACCGGTACAGTACTGCACAATCCAGCTGAAAGCAAAACGTAACAGCACCACGGTGGTGGTGAACACGCTGGCCTCAGAAAATCCGGATGAAGCCGGGCGTTACAGCATGGACGTTGAGTACGGTCAGTACAGCGTTATTCTGTTGGGGGAAGGATTACCGCCGTCACATGCCGGGCCCATTACCGTGTATGAAGTTTCTCAACCCGGTACGCTGAATg                                                                                                                                                                              >  2:259220/1‑251 (MQ=255)                                                                 agtccTGAAAGACGGCACAGGAAAACCGGTACAGAACTGCACCATTCAGCTGAAAGCCAGACGTAACAGCACCACGGTGGTGGTGAACACGGTGGGCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCGGCAGGTTGACGGTTTTCGACCATCGCACGCCGGGACCATCACCGTGTATGCAGATTCACAACAGGGGACGCTGAATGCTTTtctct                                                                                                                                                                     >  2:261102/5‑250 (MQ=255)                                                                    ccTGAAAGACGGCACAGGAAAACCGGTACAGAACTGCACCATTCAGCTGAAAGCCAGACGTAACAGCACCACGGTGGTGGTGAACACGGTGGGCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTtctctgt                                                                                                                                                                   <  1:268630/249‑4 (MQ=255)                                                                      tGAAAGACGGCACAGGAAAACCGGTACAGAACTGCACCATTCAGCTGAAAGCCAGACGTAACAGCACCACGGTGGTGGTGAACACGGTGGGCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGcc                                                                                                                                                                >  2:281113/1‑251 (MQ=255)                                                                              ggCACAGGAAAACCGGTACAGAACTGCACCATTCAGCTGAAAGCCAGACGTAACAGCACCACGGTGGTGGTGAACACGGTGGGCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACgg                                                                                                                                                         >  1:336756/1‑250 (MQ=255)                                                                               gCACAGGAAAACCGGTACAGAACTGCACAATCCAGCTGAAAGCAAAACGTAACAGCACCACGGTGGTGGTGAACACGCTGGCCTCAGAAAATCCGGATGAAGCCGGGCGTTACAGCATGGACGTTGAGTACGGTCAGTACAGCGTTATTCTGTTGGTGGAAGGATTCCCGCCGTCACATGCCGGGACCATTACCGTGTATGAAGATTCTCAACCCGGTACGCTGAATGATTTTCTCGGTGCCATGACgg                                                                                                                                                         >  2:236010/1‑249 (MQ=255)                                                                                  caGGAAAACCGGTACAGAACTGCACCATTCAGCTGAAAGCCAGACGTAACAGCACCACGGTGGTGGTGAACACGGTGGGCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGGGgat                                                                                                                                                    >  2:192830/1‑251 (MQ=255)                                                                                      aaaaCCGGTACAGAACTGCACAATCCAGCTGAAAGCAAAACGTAACAGCACCACGGTGGTGGTGAACACGCTGGCCTCAGAAAATCCGGATGAAGCCGGGCGTTACAGCATGGACGTTGAGTACGGTCAGTACAGCGTTATTCTGTTGGTGGAAGGATTCCCGCCGTCACATGCCGGGACCATTACCGTGTATGAAGATTCTCAACCCGGTACGCTGAATGATTTTCTCGGTGCCATGACGGAGGatgatg                                                                                                                                                >  2:8547/1‑251 (MQ=255)                                                                                          ccGGTACAGAACTGCACCATTCAGCTGAAAGCCAGACGTAACAGCACCACGGTGGTGGTGAACACGGTGGGCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCg                                                                                                                                            >  1:168910/1‑251 (MQ=255)                                                                                          acGGTACAGAACTGCACCATTCAGCTGAAAGCAAGACGTAACAGCACCACGGTGGTGGTGAACACGGTGGGCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGAAGGTTGACGGTTTTCCAACATCGCACGCCGGGACCATCACCGTGTATGAAGTTTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCg                                                                                                                                            >  1:168911/2‑251 (MQ=255)                                                                                           cGGTACAGAACTGCACAATCAAGCTGAAAGCAAAACGAAACCGCACCACGGTGGTGGTGAACACGCTGGCCTCAGAAAATCCGGATGACGCAGGGCGTTACAGCATGGACGTTGAGTACGGTCAGTACAGCGTTATTCTGTTGGTGGAAGGATTCCCGCCGTCACATGCCGGGACCATTACCGTGTATGAAGATTCTCAACCCGGTACGCTGAATGATTTTCTCGGTGCCATTACGGAGGATGATGCCCGt                                                                                                                                           >  2:181632/1‑251 (MQ=255)                                                                                              tACAGAACTGCACAATCCAGCTGAAAGCAAAACGTAACAGCACCACGGTGGTGGTGAACACGCTGGCCTCAGAAAATCCGGATGAAGCCGGGCGTTACAGCATGGACGTTGAGTACGGTCAGTACAGCGTTATTCTGTTGGTGGAAGGATTCCCGCCGTCACATGCCGGGACCATTACCGTGTATGAAGATTCTCAACCCGGTACGCTGAATGATTTTCTCGGTGCCATGACGGAGGATGATGCCCGTCCg                                                                                                                                        >  1:252208/1‑251 (MQ=255)                                                                                                cAGAACTGCACCATTCAGCTGAAAGCCAGACGTAACAGCACCACGGTGGTGGTGAACACGGTGGGCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCccggccgg                                                                                                                                       >  1:8018/1‑250 (MQ=255)                                                                                                  gAACTGCACCATTCAGCTGAAAGCCAGACGTAACAGCACCACGGTGGTGGTGAACACGGTGGGCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAgg                                                                                                                                    >  1:321511/1‑251 (MQ=255)                                                                                                  gAACTGCACAATCCAGCTGAAAGCAAAACGTAAAAGCACCACGGGGGTGGTGAACACGCTGGCCTCAGAAAATCCGGATGAAGCCGGGCGTTACAGCATGGACGTTGAGTACGGTCAGTACAGCGTTATTCTGTTGGTGGAAGGATTCCCGCCGTCACATGCCGGGACCATTACCGTGTATGAAGATTCTCAACCCGGTACGCTGAATGATTTTCTCGGTGCCATGACGGAGGATGATGCCCGTCCGGAgg                                                                                                                                    <  2:320419/251‑1 (MQ=255)                                                                                                   aaCTGCACCATTCAGCTGAAAGCCAGACGTAACAGCACCACGGTGGTGGTGAACACGGTGGGCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAAc                                                                                                                                                                                             >  2:351369/1‑193 (MQ=255)                                                                                                   aaCTGCACCATTCAGCTGAAAGCCAGACGTAACAGCACCACGGTGGTGGTGAACACGGTGGGCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAAc                                                                                                                                                                                             <  1:351369/193‑1 (MQ=255)                                                                                                   aaCTGCACCATTCAGCTGAAAGCCAGACGTAACAGCACCACGGTGGTGGTGAACACGGTGGGCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAggt                                                                                                                                   <  2:86532/251‑2 (MQ=255)                                                                                                       gCACAACCCAGCTGAAAGCAAAACGTAACAGCACCACGGTGGTGGTGAACACGCTGGCCTCAGAAAATCCGGATGAAGCCGGGCGTTACAGCATGGACGTTGAGTACGGTCAGTACAGCGTTATTCTGTTGGTGGAAGGATTCCCGCCGTCACATGCCGGGACCATTACCGTGTATGAAGATTCTCAACCCGGTACGCTGAATGAGTTTCTCGGTGCCATGACGGAGGATGATGCCCGTCCGGAGGCACTg                                                                                                                               <  1:157979/251‑1 (MQ=255)                                                                                                         accattcAGCTGAAAGCCAGACGTAACAGCACCACGGTGGTGGTGAACACGGTGGGCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAggtgctgcg                                                                                                                             >  1:115521/7‑244 (MQ=255)                                                                                                            attcAGCTGAAAGCCAGACGTAACAGCACCACGGTGGTGGTGAACACGGTGGGCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACACTCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGACTGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAggtgctgcgtcg                                                                                                                          >  1:129026/4‑241 (MQ=255)                                                                                                            attcAGCTGAAAGCCAGACGTAACAGCACCACGGTAGAGGTGAACACGGTGGGATCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGAGGAGTACGGTCAGTACAGTGTCATCCTGCAGGATGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAggtgctgcgtcg                                                                                                                          >  2:252336/4‑241 (MQ=255)                                                                                                            aTCCAGCTGAAAGCAAAACGTAACAGCACCACGGTGGTGGTGAACACGCTGGCCTCAGAAAATCCGGATGAAGCCGGGCGTTACAGCATGGACGTTGAGTACGGTCAGTACAGCGTTATTCTGTTGGTGGAAGGATTCCCGCCGTCACATGCCGGGACCATTACCGTGTATGAAGATTCTCAACCCGGTACGCTGAATGATTTTCTCGGTGCCATTCCGGAGGATGATGCCCGTCCGGAGGCACTGCGCCg                                                                                                                          >  2:192412/1‑251 (MQ=255)                                                                                                                aGCTGAAAGCAAAACGTAACAGCACCACGGTGGTGGTGAACACGCTGGCCTCAGAAAATCCGGATGAAGCCGGGCGTTACAGCATGGACGTTGAGTACGGTCAGTACAGCGTTATTCTGTTGGTGGAAGGATTCCCGCCGTCACATGCCGGGACCATTACCGTGTATGAAGATTCTCAACCCGGTACGCTGAATGATTTTCTCGGTGCCATGACGGAGGATGATGCCCGTCCGGAGGCACTGCGCCGtttt                                                                                                                      >  1:164849/1‑251 (MQ=255)                                                                                                                   tGAAAGCCAGACGTAACAGCACAACGGTGGTGGTGAACACGGTGGGCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCAACGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAGGTGCTGCGTCGTCTTGa                                                                                                                    >  1:129556/1‑250 (MQ=255)                                                                                                                    gAAAGCAAAACGTAACAGCACCACGGTGGTGGTGAACACGCTGGCCTCAGAAAATCCGGATGAAGCCGGGCGTTACAGCATGGACGTTGAGTACGGTCAGTACAGCGTTATTCTGTTGGTGGAAGGATTCCCGCCGTCACATGCCGGGACCATTACCGTGTATGAAGATTCTCAACCCg                                                                                                                                                                                          <  2:296333/179‑1 (MQ=255)                                                                                                                    gAAAGCAAAACGTAACAGCACCACGGTGGTGGCGAACACGCTGGCCTCAGAAAATCCGGATGAAGCCGGGCGTTACAGCATGGACGTTGAGTACGGTCAGTACAGCGTTATTCTGTTGGTGGAAGGATTCCCGCCGTCACATGCCGGGACCATTACCGTGTATGAAGATTCTCAACCCg                                                                                                                                                                                          >  1:296333/1‑179 (MQ=255)                                                                                                                                tAACAGCACCACGGTGGGGGTGAACACGGTGGTCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGCCGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGCTGCCCGGCCGGAGGTGCTGCGTCGTCTTGAACTGATGGTGGa                                                                                                        >  2:91613/1‑249 (MQ=255)                                                                                                                                        ccacGGGGGGGGTGAACCGGCTGGCCTCAGAAAATCCGGAGGAAGCGTGGCGTTACAGCAGGGACGTTGATTACGGTCAGTACAGCGTTATTCGGTGGGGGGAAGGATTCCCGCCGTCACATGCCGGGACCATTACCGTGTATGAAGATTCTCAACCCGGTACGCTGAATGATTTTCTCGGTGCCATGACGGAGGATGATGCCCGTCCGGAGGCACTGCGCCGTTTTGAACTGATGGTGGAAGAGGTGgcg                                                                                              <  2:318751/251‑1 (MQ=255)                                                                                                                                           cGGTGGTGGTGAACACGGTGGGCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGTCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAGGTGCTGCGTCGTCTTGAACTGCTGGTGGAAGAGGTGGCGCGt                                                                                           >  2:132350/1‑251 (MQ=255)                                                                                                                                            ggtggtggtGAACACGCTGGCCTCAGAAAATCCGGATGAAGCCGGGCGTTACAGCATGGACGTTGAGTACGGTCAGTACAGCGTTATTCTGTTGGTGGAAGGATTCCCGCCGTCACATGCCGGGACCATTACCGTGTATGAAGATTCTCAACCCGGTACGCTGAATGATTTTCTCGGTGCCATGACGGAGGACGATGCCCGTCCGGAGGCACTGCGCCGTTTTGAACTGATGGTGGAAGAGGTGGCGCGt                                                                                           >  2:76729/1‑250 (MQ=255)                                                                                                                                                 tggtgAACACGCTGGCATAAGACAATCCGGATGAAGCCGGGCGTTACAGCATGGACGTTGAGTACGGTCAGTACAGCGTTATTCTGTTGGTGGAAGGATTCCCGCCGTCACATGCCGGGACCATTACCGTGGATGAAGATTCTCAACCCGGCACGCTGAATGATTTTCTCGGTGCCATGACGGAGGATGATGCCCGTCCGGAGGCACTGCGCCGTTTTGAACTGATGGAGGAAGAGGTGGCGCGTAAagcg                                                                                     >  2:215578/1‑251 (MQ=255)                                                                                                                                                  ggtgAACACGGTGGGCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAGGTGCTGCGTCGTCTTGAACTGATGGTGGAAGAGGTGGCGCGTAACGCGt                                                                                    <  1:281113/251‑1 (MQ=255)                                                                                                                                                     gactacGCTGGCGTGAGAAAATCCGGAGGAGGCGGGGCGTTACAGCATGGAGGTGGAGTACGCTCAGTACAGCGTTATGCTGTTGGTGGAAGGATTCCCGCCGGCACATGCCGGGACCATTACCGTGTATGAAGATTCGCAACCCGTTACGCTGAATGATTTTCTCGGTGCCATGACGGAGTATGAGGCCCGGCCGGAGGGACTGCGCCGGTTTGAACTGATGGTGGAAGAGGGGGCGAGTAACGCGTcc                                                                                  <  2:20417/246‑1 (MQ=255)                                                                                                                                                      aaCACGGTGGGCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGTATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAGGTGCTGCGTCGTCTTGAACTGATGGTGGAAGAGGTGGCGCGTAACGCGTCcgt                                                                                <  1:233291/251‑2 (MQ=255)                                                                                                                                                      aaCACGGTGGGCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAGGTGCTGCGTCGGCTTGAACTGATGGTGGAAGAGGTGGCGCGTAACGCGTCcgt                                                                                <  2:190936/251‑2 (MQ=255)                                                                                                                                                          cggtgggcacAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGAACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGCGGATGATGCCCGGCCAGCGGTGCTGCGTCGTCTTGAACTGATGGTGGAAGAGGTGGCGCGTAACGCGTCCgtggtg                                                                            >  2:105976/10‑251 (MQ=255)                                                                                                                                                            gtgggcTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGACATCCTGCAGGATGACGGTTTTCCACCATCGCACGACGGGACCATCACCGTGTATGAAGATTCACAAACGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGAAGGTGCTGCGTCGTCTTGACCTGTTGGTGGAAGAGGTGGCGCGTAACGCGTCcgtgggggc                                                                          >  2:190062/6‑244 (MQ=255)                                                                                                                                                            cTGGCATCAGCAAATTCGGGTGAAGCCGGGCCTTACAGCATGGACGCTGAGCACGGTCAGTACCGCGTCATTCAGTTCGTGGAAGGATTCCCGCCGTCACATGCCGGGAACATTACCGTGTATGAAGATTCTCACCCCAGTACGCTGAATGATTTTCTCGGCGCCATGACGGAGGATGATGCCCGTCCGGAGGCACTGCGCCGTTTTGAACTGATGGTGGAAGAGGTGGCGCGAAACGCGTCCGCGGTGGc                                                                          >  2:55603/1‑251 (MQ=255)                                                                                                                                                             tGGCCTCAGAAAATCCGGATGAAGCCGGGCGTTACAGCATGGACGTTGAGTACGGTCAGTACAGCGTTATTCTGTTGGTGGAAGGATTCCCGCCGTCACATGCCGGGACCATTACCGTGTATGAAGATTCTCAACCCGGTACGCTGAATGATTTTCTCGGTGCCATGACGGAGGATGATGCCCGTCCGGAGGCACTGCGCCGTTTTGAACTGATGGTGGAAGAGGTGGCGCGTAACGCGTCCGCGGTGGc                                                                          >  2:8412/1‑250 (MQ=255)                                                                                                                                                                      aaaaTCCGGATGAAGCCGGGCGTTACAGCATGGACGTTGAGTACGGTCAGTACAGCGTTATTCTGTTGGTGGAAGGATTCCCGCCGTCAAATGCCGGGACCATTACCGTGTATGAAGATTCTCAACCCGGTACGCTGCATGATTTTCTCGGTGCCATGACGGAGGATGATGCCCGTCCGGAGGCACTGCGCCGTTTTGAACTGATGGAGGAAGAGGTGGCGCGTAACGCGTCCGCGGTGGCACAGAACACg                                                                >  1:148090/1‑251 (MQ=255)                                                                                                                                                                            cGGATGAAGCCGGGCGTTACAGCATGGACGTTGAGTACGGTCAGTACAGCGTTATTCTGTTGGTGGAAGGATTCCCGCCGTCACATGCCGGGACCATTACCGTGTATGAAGATTCTCAACCCGGTACGCTGAATGATTTTCTCGGTGCCATGACGGAGGATGATGCCCGTCCGGAGGCACTGCGCCGTTTTGAACTGATGGTGGAAGAGGTGGCGCGTAACGCGTCCGCGGTGGCACAGAACACGGCAGcc                                                          >  1:190553/1‑251 (MQ=255)                                                                                                                                                                            cGGATGAAGACGGGCGTTACAGCATGGATGTGGAGAACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCAACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGACTGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAGGTGCTGCGTCGTCTTGAACTGATGGTGGAAGAGGTGGCGCGTAACGCGTCCGTGGTGGCACAGAGTACGGCAgac                                                          >  2:176546/1‑249 (MQ=255)                                                                                                                                                                             ggaggAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAGGTGCTGCGTCGTCTTGAACTGATGGTGGAAGAGGTGGCGCGTAACGCGTCCGTGGTGGCACAGAGTACGGCAgacg                                                         <  2:276128/247‑4 (MQ=255)                                                                                                                                                                             ggATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAGGTGCTGCGTCGTCTTGAACTGATGGTGGAAGAGGTGGCGCGTAACGCGTCCGTGGTGGCACAGAGTACGGCAgacg                                                         >  1:106096/1‑248 (MQ=255)                                                                                                                                                                                      cGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACTGTTTTACACCATCGCACGCCGGGACCATCACCTTTTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAGGTGCTGCGTCGTCTTGAACTGATGGTGGAAGAGGTGGCGCGTAACGCGTACGTGGTGGCACAGAGTACGGCATACGCgaagaaat                                                <  1:287257/251‑3 (MQ=255)                                                                                                                                                                                         gCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAGGTGCTGCGTCGTCTTGAACTGATGGTGGAAGAGGTGGCGCGTAACGCGTCCGTGGTGGCACAGAGTACGGCAGACGCGAAGAAATCAg                                             <  2:245726/251‑1 (MQ=255)                                                                                                                                                                                             tACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAGGTGCTGCGTCGTCTTGAACTGATGGTGGAAGAGGTGGCGCGTAACGCGTCCGTGGTGGCACAGAGTACGGCAGACGCGAAGAAATCAGccgg                                         >  1:203506/1‑249 (MQ=255)                                                                                                                                                                                                   aTGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTCTGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAGGTGCTGCGTCGTCTTGAACTGATGGTGGAAGAGGTGGCGCGTAACGCGTCCGTGGTGGCACAGAGTACGGCAGACGCGAAGAAATCAGCCGGCGATGc                                   >  2:31913/1‑251 (MQ=255)                                                                                                                                                                                                    tggatgtggAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAGGTGCTGCGTCGTCTTGAACTGATGGTGGAAGAGGTGGCGCGTAACGCGTCCGTGGTGGCACAGAGTACGGCAGACGCGAAGCAATCAGCCGGCGATGcc                                  >  2:318646/9‑251 (MQ=255)                                                                                                                                                                                                      gatgtggAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAGGTGCTGCGTCGTCTTGAACTGATGGTGGAAGAGGTGGCGCGTAACGCGTCCGTGGTGGCACAGAGTACGGCAGACGCGCAGAAATCAGCCGGCGATGcc                                  >  1:326441/7‑249 (MQ=255)                                                                                                                                                                                                             aGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAGGTGCTGCGTCGTCTTGAACTGATGGTGGAAGAGGTGGCGCGTAACGCGTCCGTGGTGGCACAGAGTACGGCAGACGCGAAGAAATCAGCCGGCGATGCCAgtgcatc                          >  2:335518/1‑244 (MQ=255)                                                                                                                                                                                                              gTACGGTCAGTCCAGAGTTATTCTGTTGGTGGAAGGATTCCCGCCGTCACATGCCGGGCCCATTACCGTGTATGAAGATTCTCAACCCGGTACGCTGAATGATTTTCTCGGTGCCATGACGGAGGATGATGCCCGTCCGGAGGCACTGCGCCGTTTTGAACTGATGGAGGACGAGGTGGCGCGTACCGCGTCCGCGGTGGCACAGAACACGGCAGCCGCGAAGCAGTCAGCCAGTGATGCCAGCACATc                          >  2:216535/1‑249 (MQ=255)                                                                                                                                                                                                                  ggTCAGTACAGCGTTATTCTGTTGGTGGAAGGATTCCCGCCGTCACATGCCGGGACCATTACCGTGTATGAAGATTCTCAACCCGGTACGCTGAATGATTTTCTCGGTGCCATGACGGAGGATGATGCCCGTCCGGAGGCACTGCGCCGTTTTGAACTGATGGTGGAAGAGGTGGCGCGTAACGCGTCCGCGGGGGCACAGAACACGGCAGCCGCGAAGAAGTCAGCCAGGGATGCCAGCACATCAGCCCg                    >  2:198789/1‑251 (MQ=255)                                                                                                                                                                                                                     cAGTACAGCGTTATTCTGTTGGTGGAAGGATTCCCGCCGTCACATGCCGGGACCATTACCGTGTATGAAGATTCTCAACCCGGTACGCTGAATGATTTTCTCGGTGCCATGACGGAGGATGATGCCCGTCCGGAGGCACTGCGCCGTTTTGAACTGATGGTGGAAGAGGTGGCGCGTAACGCGTCCGCGGTGGCACAGAACACGGCAGCCGCGAAGAAGTCAGCCAGTGATGCCAGCACATCAGCCCGTGa                 <  1:291443/251‑1 (MQ=255)                                                                                                                                                                                                                         aCAGCGTTATTCTGTTGGTGGAAGGATTCCCGCCGTCACATGCCGGGACCATTACCGTGTATGAAGATTCTCAACCCGGTACGCTGAATGATTTTCTCGGTGCCATGCCGGCGGATGATGCCCGTCCGGCGGCACTGCGCCGTTTTGAACTGATGGTGGCAGAGGTGGCGCGTAACGCGTCCGCGGTGGCACAAAACACGGCAGCCGCGAAGAAGTCAGCCAGTGATGCCAGCACATCAACCCGTGAggcg             >  1:344114/1‑251 (MQ=255)                                                                                                                                                                                                                           aGCGTTATTCTGTTGGTGGAAGGATTCCCGCCGTCACATGCCGGGACCATTACCGTGTATGAAGATTCTCAACCCGGTACGCTGAATGATTTTCTCGGTGCCATGACGGAGGATGATGCCCGTCCGGAGGCCCTGCGCCGTTTTGAACTGATGGTGGAAGAGGTGGCGCGTAACGCGTCCGCGGTGGCACAGAACACGGCAGCCGCGCAGAAGTCAGCCAGTGATGCCAGCACATCAACCCGTGAggcggc           >  1:113556/1‑251 (MQ=255)                                                                                                                                                                                                                                 aTTCTGTTGGTGGAAGTATTCCCGCCGTCACATGCCGGGACCATTACCGTGTATGAAGATTCTCAACCCGGTACGCTGAATGATTTTCTCGGTGCCATGACGGAGGATGATGCCCGTCCGGAGGCACTGCGCCGTTTTGAACTGATGGTGGAAGAGGTGGCGCGTAACGCGTCCGCGGTGGCACAGAACACGGCAGCCGCGAAGAAGTCAGCCAGTGATGCCAGCACATCAGCCCGTGAGGCGGCAAccc      <  1:31399/250‑1 (MQ=255)                                                                                                                                                                                                                                    atGTTGGTGGAAGGATTCCCGCCATCACATGCCGGGACCATTACCGTGTATGAAGATTCTCAACCAGGTACGCTGAATGATTTTCTCGGTGCCATGACGGAGGATGATGCCCGTCCGGAGGCACTGCGCCGTTTTGAACTGATGGTGGACGAGGTGGCGCGTAACGCGTCCGCGGTGGCACAGCACAAGGCAGCCGCGAAGAAGTCAGCCACTGATTCCAGCAAATCAGCCCGTGAGACGGCCACCAATGc  >  2:185470/2‑251 (MQ=255)                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                       TATGACAGCCCGCCGGTTCAGGCGGGCTTTTTTGTGGGGTGAATATGGCAGTAAAGATTTCAGGTGTACTGAAAGACGGCACAGGAAAACCGGTACAGAACTGCACAATCCAGCTGAAAGCAAAACGTAACAGCACCACGGTGGTGGTGAACACGCTGGCCTCAGAAAATCCGGATGAAGCCGGGCGTTACAGCATGGACGTTGAGTACGGTCAGTACAGCGTTATTCTGTTGGTGGAAGGATTCCCGCCGTCACATGCCGGGACCATTACCGTGTATGAAGATTCTCAACCCGGTACGCTGAATGATTTTCTCGGTGCCATGACGGAGGATGATGCCCGTCCGGAGGCACTGCGCCGTTTTGAACTGATGGTGGAAGAGGTGGCGCGTAACGCGTCCGCGGTGGCACAGAACACGGCAGCCGCGAAGAAGTCAGCCAGTGATGCCAGCACATCAGCCCGTGAGGCGGCAACCCATGC  >  NC_000913/1429005‑1429482

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑