Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,429,2070TG56.7% 45.7 / 73.0 67V53V (GTT→GTG) stfRputative prophage side tail fiber protein StfR
Reads supporting (aligned to +/- strand):  ref base T (19/10);  new base G (24/14);  total (43/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

TGTCGGTTATAAGTTCTGATTAGCCAGGTAACACAGTGTTATGACAGCCCGCCGGTTCAGGCGGGCTTTTTTGTGGGGTGAATATGGCAGTAAAGATTTCAGGTGTACTGAAAGACGGCACAGGAAAACCGGTACAGAACTGCACAATCCAGCTGAAAGCAAAACGTAACAGCACCACGGTGGTGGTGAACACGCTGGCCTCAGAAAATCCGGATGAAGCCGGGCGTTACAGCATGGACGTTGAGTACGGTCAGTACAGCGTTATTCTGTTGGTGGAAGGATTCCCGCCGTCACATGCCGGGACCATTACCGTGTATGAAGATTCTCAACCCGGTACGCTGAATGATTTTCTCGGTGCCATGACGGAGGATGATGCCCGTCCGGAGGCACTGCGCCGTTTTGAACTGATGGTGGAAGAGGTGGCGCGTAACGCGTCCGCGGTGGCACAGAACACGGCAGCCGCGAAGAAGTCAGCCAGTGATGC  >  NC_000913/1428966‑1429449
                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                  
tgtCGGTTATAAGGTCTGATTAGCCAGGTAACACAGTGTTATGACAGCCCGCCGGTTAAGGCGGGCTTTTTTGTGGGGTGAATATGGCAGAAAAGATTTCAGGTGTACTGAAAGACGGCCCAGGAAAACCGGTACAGAACTGCACAATCCAGCTGAAAGCAAAACGTAACAGCACCACGGTGGTGGTGAACACGCTGGCCTCAGAAAATCCGGATGAAACCGGGCGTTACAGCATGGACGTTGAGTACGGt                                                                                                                                                                                                                                           >  2:139766/1‑251 (MQ=255)
       tataAATTCTGATTAGCCAGGTAACACAGTGTTATGACAGCCCGCCGGAACCGGTGGGCTTTTTTGTGGGGTGGATATGGCAGTAAAGATTTCAGGAGTCCTGAAAGACGGCACAGGAAAACCGGTACAGAACTGCACCATTCAGCTGAAAGCCAGACGTAACAGCACCACGGTGGTGGTGAACACGGTGGGCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTAc                                                                                                                                                                                                                                     <  1:209358/250‑1 (MQ=255)
        ataAGTGCTGATTAGCCAGGTAACACGGTGTTATGACAGCCCGCCGGTTCAGGCGGGCTTTTTTGTGGGGTGAATATGGCAGTAAAGATTTCAGGTGTACTGAAAGACGGCACAGGAAAACCGGTACAGAACTGCACAATCCAGCTGAAAGCAAAACGTAACAGCACCACGGTGGTGGTGAACACGCTGGCCTCAGAAAATCCGGATGAAGCCGGGCGTTACAGCATGGACGTTGAGTACGGTCAGTACAg                                                                                                                                                                                                                                   <  2:285102/251‑1 (MQ=255)
                tGATTAGCCAGGTAACACAGTGTTATGACAGCCCGCCGGTTCAGGCGGGCTTTTTTGTGGGGTGAATATGGCAGTAAAGATTTCAGGTGTACTGAAAGACGGCACAGGAAAACCGGTACAGAACTGCACAATCCAGCTGAAAGCAAAACGTAACAGCACCACGGTGGTGGTGAACACGCTGGCCTCAGAAAATCCGGATGAAGCCGGGCGGTACAGCATGGACGTTGAGAACGGTCAGTACAGCGTTATTc                                                                                                                                                                                                                           >  2:255003/1‑251 (MQ=255)
                             aaCACAGTGTTATGACAGCCCGCCGGAACCGGTGGGCTTTTTTGTGGGGTGAAGAGGGCAGTAAAGATTTCAGGAGATCTGAAAGACTGCACATGAAAACCGGTTCAGAACTGCACCATTCAGCTGAAAGCCAGACGTAACAGCACCACGGTGGTGGTGAACACTGTGGGCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGACGTGGAGTACGGTCAGTACAgtgtcatcctgcaggttgacgg                                                                                                                                                                                                              <  1:319908/251‑22 (MQ=255)
                              acacAGTGTTATGACAGCCCGCCGGAACCGGTGGGCTTTTTTGTGGGGTGAATATGGCAGTAAAGATTTCAGGAGTCCTGAAAGACGGCACAGGAAAACCGGTACAGAACTGCACCATTCAGCTGAAAGCCAGACGTAACAGCACCACGGTGGTGGTGAACACGGTGGGCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAgtgtcatcctgcaggttgacggt                                                                                                                                                                                                             >  1:245726/1‑229 (MQ=255)
                                       gaTGACAGCCCGCCGGTTCAGGCGGGGTTTTTTGTGGGGTGAATATGGCAGTAAAGATTTCAGGTGTACTGAAAGACAGCACAGGAAAACCGGTACAGAACTGCACAATCCAGCTGAAAGCAAAACGTAACAGCACCACGGTGGTGTTGAACACTCTGGCCTCAGAAAATCCGGAGGAAGCCGGGCGTTACAGCATGGACGTTGAGTACGGTCAGTACAGCGTTATTCTGTTGGTGGAAGGATTCccgcc                                                                                                                                                                                                     <  1:323807/249‑1 (MQ=255)
                                            cAGCCCGCCGGTTCAGGCGGGCTTTTTTGTGGGGTGAATATGGCAGTAAAGCTTTCAGGTGTACTGAAAGACGGCACAGGAAAACCGGTACAGAACTGCACAATCCAGCTGAAAGCAAACCGTAACAACACCCCGGTGGTGGTGAAAACGCTGGCCTCAGAAAATCCGGATGAAGCCGGGCGTTACAGCATGGACGTTGAGTACGGTCAGTACAGCGTTATTCTGTTGGTGGAAGGATTCCCGCCGTcac                                                                                                                                                                                                >  1:323834/1‑250 (MQ=255)
                                                     ggTTCAGGCGGGCTTTTTTGTGGGGTGAATATGGCAGTAAAGATTTCAGGTGTACTGAAAGACGGCACAGGAAAACCGGTACAGAACTGCACAATCCAGCTGAAAGCAAAACGTAACAGCACCACGGTGGTGGTGAACACGCTGGCCTCAGAAAATCCGGATGAAGCCGGGCGTTACAGCATGGACGTTGAGTACGGTCAGTACAGCGTTATTCTGTTGGTGGAAGGATTCCCGCCGTCACATGCCGGGAc                                                                                                                                                                                      >  1:86022/1‑251 (MQ=255)
                                                           ggCGGGCTTTTTTGTGGGGTGAATATGGCAGTAAAGATTGCAGGTGTACTGAAAGACGGCACAGGAAAACCGGTACATAACTGCACAATCCAGCTGAAAGCAAAACGTAACAGCACCACGGGGGTGGGGAACAGGCTGGCCTCAGAAAATCGGGATGAAGCCGGGCTTTATAGCATGGACGTTGAGTACGGTCATTACAGCGTTACTCTGTTGTTGGAAGGATTCCCGCCGTCACATGCCGGGACCATTAc                                                                                                                                                                                <  1:212371/251‑1 (MQ=255)
                                                                gCTTTTTTGTGGGGTAAATATGGCAGTAAAGATTTCAGGTTAACGGAAAGACGGCACAGGAAAACCGGTACAGAACGGCAAAATCCAGCTGAAAGCAAAACGTAACAGCCCCACGGTTGTGGTGAACACGCTGGCCTCATAAAATCCGGATGAAGCCGGGCGTTACATCATGGACGTTGAGTACGGTCAGTACAGCGTTATTCTGTTGGTGGAAGGATTCCCGCCGTCACATGCCGGGACCATTACCgtgt                                                                                                                                                                           <  1:24294/251‑1 (MQ=255)
                                                                        gtgGGGTGAATATGGCAGTAAAGATTTCAGGAGTCCTGAAAGACGGCACAGGAAAACCGGTACAGAACTGCACCATTCAGCTGAAAGCCAGACGTAACAGCACCACGGTGGTGGTGAACACGGTGGGCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGAt                                                                                                                                                                   <  1:8352/251‑1 (MQ=255)
                                                                          ggggTGAATATGGCAGTAAAGATTTCAGGTGTACTGAAAGACGGCACAGGAAACCCGGTACAGAACTGCACAATCCAGCTGAAAGCAAAACGTACCAGCACCACGGTGGTGGTGAACACGCTGGCCTCAGAAAATCCGGATGAAGCCGGGCGTTACAGCATGGACGTTGAGTACGGTCAGTACAGCGTTATTCTGTTGGTGGAAGGATTCCCGCCGTCACATGCCGGGACCATTACCGTGTATGAAGATtc                                                                                                                                                                 <  2:63714/251‑1 (MQ=255)
                                                                             gTGAATATGGCAGTAAAGATTTCAGGAGTCCTGAAAGACGGCACAGGAAAACCGGTACAGAACTGCACCATTCAGCTGAAAGCCAGACGTAACAGCACCACGGTGGTGGTGAACACGGTGGGCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATtcac                                                                                                                                                               >  1:190936/1‑248 (MQ=255)
                                                                                 atatGGCAGTAAAGATTTCAGGAGTCCTGAAAGACGGCACAGGAAAACCGGTACAGAACTGCACCATTCAGCTGAAAGCCAGACGTAACAGCACCACGGTGGTGGTGCACACGGTGGGCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAAccg                                                                                                                                                          >  2:177041/1‑250 (MQ=255)
                                                                                   atGGCAGTAAAGATTTCAGGAGTCCTGAAAGACGGCACAGGAAAACCGGTACAGAACTGCACCATTCAGCTGAAAGCCAGACGTAACAGCACCACGGTGGTGGTGAACACGGTGGGCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAAccggg                                                                                                                                                        <  1:119242/251‑4 (MQ=255)
                                                                                       cAGTAAAGATTTCAGGTGTACTGAAAGACGGCACAGGAAAACCGGTACAGAACTGCACAATCCAGCTGAAAGCAAAACGTAACAGCACCACGGTGGTGGTGAACACGCTGGCCTCAGAAAATCCGGATGAAGCCGGGCGTTACAGCATGGACGTTGAGTACGGTCAGTACAGCGTTATTCTGTTGGTGGAAGGATTCCCGCCGTCACATGCCGGGACCATTACCGTGTATGAAGATTCTCAACCCGGTACg                                                                                                                                                    >  2:31399/1‑251 (MQ=255)
                                                                                         gTAAAGATTTCAGGTGTACTGAAAGACGGCACAGGAAAACCGGTACAGAACTGCACAATCCAGCTGAAAGCAAAACGTAACAGCACCACGGTGGTGGTGAACACGCTGGCCTCAGAAAATCCGGATGAAGCCGGGCGTTACAGCATGGACGTTGAGTACGGTCAGTACAGCGTTATTCTGTTGGTGGAAGGATTCCCGCCGTCACATGCCGGGACCATTACCGTGTATGAAGATTCTCAACCCGGTACGCt                                                                                                                                                  <  1:19093/251‑1 (MQ=255)
                                                                                              gATTTCAGGTGTACTGAAAGACGGCACAGGAACACCGGTACAGTACTGCACAATCCAGCTGAAAGCAAAACGTAACAGCACCACGGTGGTGGTGAACACGCTGGCCTCAGAAAATCCGGATGAAGCCGGGCGTTACAGCATGGACGTTGAGTACGGTCAGTACAGCGTTATTCTGTTGGGGGAAGGATTACCGCCGTCACATGCCGGGCCCATTACCGTGTATGAAGTTTCTCAACCCGGTACGCTGAATg                                                                                                                                             >  2:259220/1‑251 (MQ=255)
                                                                                                       agtccTGAAAGACGGCACAGGAAAACCGGTACAGAACTGCACCATTCAGCTGAAAGCCAGACGTAACAGCACCACGGTGGTGGTGAACACGGTGGGCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCGGCAGGTTGACGGTTTTCGACCATCGCACGCCGGGACCATCACCGTGTATGCAGATTCACAACAGGGGACGCTGAATGCTTTtctct                                                                                                                                    >  2:261102/5‑250 (MQ=255)
                                                                                                          ccTGAAAGACGGCACAGGAAAACCGGTACAGAACTGCACCATTCAGCTGAAAGCCAGACGTAACAGCACCACGGTGGTGGTGAACACGGTGGGCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTtctctgt                                                                                                                                  <  1:268630/249‑4 (MQ=255)
                                                                                                            tGAAAGACGGCACAGGAAAACCGGTACAGAACTGCACCATTCAGCTGAAAGCCAGACGTAACAGCACCACGGTGGTGGTGAACACGGTGGGCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGcc                                                                                                                               >  2:281113/1‑251 (MQ=255)
                                                                                                                    ggCACAGGAAAACCGGTACAGAACTGCACCATTCAGCTGAAAGCCAGACGTAACAGCACCACGGTGGTGGTGAACACGGTGGGCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACgg                                                                                                                        >  1:336756/1‑250 (MQ=255)
                                                                                                                     gCACAGGAAAACCGGTACAGAACTGCACAATCCAGCTGAAAGCAAAACGTAACAGCACCACGGTGGTGGTGAACACGCTGGCCTCAGAAAATCCGGATGAAGCCGGGCGTTACAGCATGGACGTTGAGTACGGTCAGTACAGCGTTATTCTGTTGGTGGAAGGATTCCCGCCGTCACATGCCGGGACCATTACCGTGTATGAAGATTCTCAACCCGGTACGCTGAATGATTTTCTCGGTGCCATGACgg                                                                                                                        >  2:236010/1‑249 (MQ=255)
                                                                                                                        caGGAAAACCGGTACAGAACTGCACCATTCAGCTGAAAGCCAGACGTAACAGCACCACGGTGGTGGTGAACACGGTGGGCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGGGgat                                                                                                                   >  2:192830/1‑251 (MQ=255)
                                                                                                                            aaaaCCGGTACAGAACTGCACAATCCAGCTGAAAGCAAAACGTAACAGCACCACGGTGGTGGTGAACACGCTGGCCTCAGAAAATCCGGATGAAGCCGGGCGTTACAGCATGGACGTTGAGTACGGTCAGTACAGCGTTATTCTGTTGGTGGAAGGATTCCCGCCGTCACATGCCGGGACCATTACCGTGTATGAAGATTCTCAACCCGGTACGCTGAATGATTTTCTCGGTGCCATGACGGAGGatgatg                                                                                                               >  2:8547/1‑251 (MQ=255)
                                                                                                                                ccGGTACAGAACTGCACCATTCAGCTGAAAGCCAGACGTAACAGCACCACGGTGGTGGTGAACACGGTGGGCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCg                                                                                                           >  1:168910/1‑251 (MQ=255)
                                                                                                                                acGGTACAGAACTGCACCATTCAGCTGAAAGCAAGACGTAACAGCACCACGGTGGTGGTGAACACGGTGGGCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGAAGGTTGACGGTTTTCCAACATCGCACGCCGGGACCATCACCGTGTATGAAGTTTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCg                                                                                                           >  1:168911/2‑251 (MQ=255)
                                                                                                                                 cGGTACAGAACTGCACAATCAAGCTGAAAGCAAAACGAAACCGCACCACGGTGGTGGTGAACACGCTGGCCTCAGAAAATCCGGATGACGCAGGGCGTTACAGCATGGACGTTGAGTACGGTCAGTACAGCGTTATTCTGTTGGTGGAAGGATTCCCGCCGTCACATGCCGGGACCATTACCGTGTATGAAGATTCTCAACCCGGTACGCTGAATGATTTTCTCGGTGCCATTACGGAGGATGATGCCCGt                                                                                                          >  2:181632/1‑251 (MQ=255)
                                                                                                                                    tACAGAACTGCACAATCCAGCTGAAAGCAAAACGTAACAGCACCACGGTGGTGGTGAACACGCTGGCCTCAGAAAATCCGGATGAAGCCGGGCGTTACAGCATGGACGTTGAGTACGGTCAGTACAGCGTTATTCTGTTGGTGGAAGGATTCCCGCCGTCACATGCCGGGACCATTACCGTGTATGAAGATTCTCAACCCGGTACGCTGAATGATTTTCTCGGTGCCATGACGGAGGATGATGCCCGTCCg                                                                                                       >  1:252208/1‑251 (MQ=255)
                                                                                                                                      cAGAACTGCACCATTCAGCTGAAAGCCAGACGTAACAGCACCACGGTGGTGGTGAACACGGTGGGCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCccggccgg                                                                                                      >  1:8018/1‑250 (MQ=255)
                                                                                                                                        gAACTGCACCATTCAGCTGAAAGCCAGACGTAACAGCACCACGGTGGTGGTGAACACGGTGGGCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAgg                                                                                                   >  1:321511/1‑251 (MQ=255)
                                                                                                                                        gAACTGCACAATCCAGCTGAAAGCAAAACGTAAAAGCACCACGGGGGTGGTGAACACGCTGGCCTCAGAAAATCCGGATGAAGCCGGGCGTTACAGCATGGACGTTGAGTACGGTCAGTACAGCGTTATTCTGTTGGTGGAAGGATTCCCGCCGTCACATGCCGGGACCATTACCGTGTATGAAGATTCTCAACCCGGTACGCTGAATGATTTTCTCGGTGCCATGACGGAGGATGATGCCCGTCCGGAgg                                                                                                   <  2:320419/251‑1 (MQ=255)
                                                                                                                                         aaCTGCACCATTCAGCTGAAAGCCAGACGTAACAGCACCACGGTGGTGGTGAACACGGTGGGCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAAc                                                                                                                                                            >  2:351369/1‑193 (MQ=255)
                                                                                                                                         aaCTGCACCATTCAGCTGAAAGCCAGACGTAACAGCACCACGGTGGTGGTGAACACGGTGGGCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAAc                                                                                                                                                            <  1:351369/193‑1 (MQ=255)
                                                                                                                                         aaCTGCACCATTCAGCTGAAAGCCAGACGTAACAGCACCACGGTGGTGGTGAACACGGTGGGCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAggt                                                                                                  <  2:86532/251‑2 (MQ=255)
                                                                                                                                             gCACAACCCAGCTGAAAGCAAAACGTAACAGCACCACGGTGGTGGTGAACACGCTGGCCTCAGAAAATCCGGATGAAGCCGGGCGTTACAGCATGGACGTTGAGTACGGTCAGTACAGCGTTATTCTGTTGGTGGAAGGATTCCCGCCGTCACATGCCGGGACCATTACCGTGTATGAAGATTCTCAACCCGGTACGCTGAATGAGTTTCTCGGTGCCATGACGGAGGATGATGCCCGTCCGGAGGCACTg                                                                                              <  1:157979/251‑1 (MQ=255)
                                                                                                                                               accattcAGCTGAAAGCCAGACGTAACAGCACCACGGTGGTGGTGAACACGGTGGGCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAggtgctgcg                                                                                            >  1:115521/7‑244 (MQ=255)
                                                                                                                                                  attcAGCTGAAAGCCAGACGTAACAGCACCACGGTGGTGGTGAACACGGTGGGCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACACTCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGACTGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAggtgctgcgtcg                                                                                         >  1:129026/4‑241 (MQ=255)
                                                                                                                                                  attcAGCTGAAAGCCAGACGTAACAGCACCACGGTAGAGGTGAACACGGTGGGATCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGAGGAGTACGGTCAGTACAGTGTCATCCTGCAGGATGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAggtgctgcgtcg                                                                                         >  2:252336/4‑241 (MQ=255)
                                                                                                                                                  aTCCAGCTGAAAGCAAAACGTAACAGCACCACGGTGGTGGTGAACACGCTGGCCTCAGAAAATCCGGATGAAGCCGGGCGTTACAGCATGGACGTTGAGTACGGTCAGTACAGCGTTATTCTGTTGGTGGAAGGATTCCCGCCGTCACATGCCGGGACCATTACCGTGTATGAAGATTCTCAACCCGGTACGCTGAATGATTTTCTCGGTGCCATTCCGGAGGATGATGCCCGTCCGGAGGCACTGCGCCg                                                                                         >  2:192412/1‑251 (MQ=255)
                                                                                                                                                      aGCTGAAAGCAAAACGTAACAGCACCACGGTGGTGGTGAACACGCTGGCCTCAGAAAATCCGGATGAAGCCGGGCGTTACAGCATGGACGTTGAGTACGGTCAGTACAGCGTTATTCTGTTGGTGGAAGGATTCCCGCCGTCACATGCCGGGACCATTACCGTGTATGAAGATTCTCAACCCGGTACGCTGAATGATTTTCTCGGTGCCATGACGGAGGATGATGCCCGTCCGGAGGCACTGCGCCGtttt                                                                                     >  1:164849/1‑251 (MQ=255)
                                                                                                                                                         tGAAAGCCAGACGTAACAGCACAACGGTGGTGGTGAACACGGTGGGCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCAACGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAGGTGCTGCGTCGTCTTGa                                                                                   >  1:129556/1‑250 (MQ=255)
                                                                                                                                                          gAAAGCAAAACGTAACAGCACCACGGTGGTGGTGAACACGCTGGCCTCAGAAAATCCGGATGAAGCCGGGCGTTACAGCATGGACGTTGAGTACGGTCAGTACAGCGTTATTCTGTTGGTGGAAGGATTCCCGCCGTCACATGCCGGGACCATTACCGTGTATGAAGATTCTCAACCCg                                                                                                                                                         <  2:296333/179‑1 (MQ=255)
                                                                                                                                                          gAAAGCAAAACGTAACAGCACCACGGTGGTGGCGAACACGCTGGCCTCAGAAAATCCGGATGAAGCCGGGCGTTACAGCATGGACGTTGAGTACGGTCAGTACAGCGTTATTCTGTTGGTGGAAGGATTCCCGCCGTCACATGCCGGGACCATTACCGTGTATGAAGATTCTCAACCCg                                                                                                                                                         >  1:296333/1‑179 (MQ=255)
                                                                                                                                                                      tAACAGCACCACGGTGGGGGTGAACACGGTGGTCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGCCGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGCTGCCCGGCCGGAGGTGCTGCGTCGTCTTGAACTGATGGTGGa                                                                       >  2:91613/1‑249 (MQ=255)
                                                                                                                                                                              ccacGGGGGGGGTGAACCGGCTGGCCTCAGAAAATCCGGAGGAAGCGTGGCGTTACAGCAGGGACGTTGATTACGGTCAGTACAGCGTTATTCGGTGGGGGGAAGGATTCCCGCCGTCACATGCCGGGACCATTACCGTGTATGAAGATTCTCAACCCGGTACGCTGAATGATTTTCTCGGTGCCATGACGGAGGATGATGCCCGTCCGGAGGCACTGCGCCGTTTTGAACTGATGGTGGAAGAGGTGgcg                                                             <  2:318751/251‑1 (MQ=255)
                                                                                                                                                                                 cGGTGGTGGTGAACACGGTGGGCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGTCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAGGTGCTGCGTCGTCTTGAACTGCTGGTGGAAGAGGTGGCGCGt                                                          >  2:132350/1‑251 (MQ=255)
                                                                                                                                                                                  ggtggtggtGAACACGCTGGCCTCAGAAAATCCGGATGAAGCCGGGCGTTACAGCATGGACGTTGAGTACGGTCAGTACAGCGTTATTCTGTTGGTGGAAGGATTCCCGCCGTCACATGCCGGGACCATTACCGTGTATGAAGATTCTCAACCCGGTACGCTGAATGATTTTCTCGGTGCCATGACGGAGGACGATGCCCGTCCGGAGGCACTGCGCCGTTTTGAACTGATGGTGGAAGAGGTGGCGCGt                                                          >  2:76729/1‑250 (MQ=255)
                                                                                                                                                                                       tggtgAACACGCTGGCATAAGACAATCCGGATGAAGCCGGGCGTTACAGCATGGACGTTGAGTACGGTCAGTACAGCGTTATTCTGTTGGTGGAAGGATTCCCGCCGTCACATGCCGGGACCATTACCGTGGATGAAGATTCTCAACCCGGCACGCTGAATGATTTTCTCGGTGCCATGACGGAGGATGATGCCCGTCCGGAGGCACTGCGCCGTTTTGAACTGATGGAGGAAGAGGTGGCGCGTAAagcg                                                    >  2:215578/1‑251 (MQ=255)
                                                                                                                                                                                        ggtgAACACGGTGGGCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAGGTGCTGCGTCGTCTTGAACTGATGGTGGAAGAGGTGGCGCGTAACGCGt                                                   <  1:281113/251‑1 (MQ=255)
                                                                                                                                                                                           gactacGCTGGCGTGAGAAAATCCGGAGGAGGCGGGGCGTTACAGCATGGAGGTGGAGTACGCTCAGTACAGCGTTATGCTGTTGGTGGAAGGATTCCCGCCGGCACATGCCGGGACCATTACCGTGTATGAAGATTCGCAACCCGTTACGCTGAATGATTTTCTCGGTGCCATGACGGAGTATGAGGCCCGGCCGGAGGGACTGCGCCGGTTTGAACTGATGGTGGAAGAGGGGGCGAGTAACGCGTcc                                                 <  2:20417/246‑1 (MQ=255)
                                                                                                                                                                                            aaCACGGTGGGCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGTATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAGGTGCTGCGTCGTCTTGAACTGATGGTGGAAGAGGTGGCGCGTAACGCGTCcgt                                               <  1:233291/251‑2 (MQ=255)
                                                                                                                                                                                            aaCACGGTGGGCTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAGGTGCTGCGTCGGCTTGAACTGATGGTGGAAGAGGTGGCGCGTAACGCGTCcgt                                               <  2:190936/251‑2 (MQ=255)
                                                                                                                                                                                                cggtgggcacAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGAACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGCGGATGATGCCCGGCCAGCGGTGCTGCGTCGTCTTGAACTGATGGTGGAAGAGGTGGCGCGTAACGCGTCCgtggtg                                           >  2:105976/10‑251 (MQ=255)
                                                                                                                                                                                                  gtgggcTCAGAGAATCCGGATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGACATCCTGCAGGATGACGGTTTTCCACCATCGCACGACGGGACCATCACCGTGTATGAAGATTCACAAACGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGAAGGTGCTGCGTCGTCTTGACCTGTTGGTGGAAGAGGTGGCGCGTAACGCGTCcgtgggggc                                         >  2:190062/6‑244 (MQ=255)
                                                                                                                                                                                                  cTGGCATCAGCAAATTCGGGTGAAGCCGGGCCTTACAGCATGGACGCTGAGCACGGTCAGTACCGCGTCATTCAGTTCGTGGAAGGATTCCCGCCGTCACATGCCGGGAACATTACCGTGTATGAAGATTCTCACCCCAGTACGCTGAATGATTTTCTCGGCGCCATGACGGAGGATGATGCCCGTCCGGAGGCACTGCGCCGTTTTGAACTGATGGTGGAAGAGGTGGCGCGAAACGCGTCCGCGGTGGc                                         >  2:55603/1‑251 (MQ=255)
                                                                                                                                                                                                   tGGCCTCAGAAAATCCGGATGAAGCCGGGCGTTACAGCATGGACGTTGAGTACGGTCAGTACAGCGTTATTCTGTTGGTGGAAGGATTCCCGCCGTCACATGCCGGGACCATTACCGTGTATGAAGATTCTCAACCCGGTACGCTGAATGATTTTCTCGGTGCCATGACGGAGGATGATGCCCGTCCGGAGGCACTGCGCCGTTTTGAACTGATGGTGGAAGAGGTGGCGCGTAACGCGTCCGCGGTGGc                                         >  2:8412/1‑250 (MQ=255)
                                                                                                                                                                                                            aaaaTCCGGATGAAGCCGGGCGTTACAGCATGGACGTTGAGTACGGTCAGTACAGCGTTATTCTGTTGGTGGAAGGATTCCCGCCGTCAAATGCCGGGACCATTACCGTGTATGAAGATTCTCAACCCGGTACGCTGCATGATTTTCTCGGTGCCATGACGGAGGATGATGCCCGTCCGGAGGCACTGCGCCGTTTTGAACTGATGGAGGAAGAGGTGGCGCGTAACGCGTCCGCGGTGGCACAGAACACg                               >  1:148090/1‑251 (MQ=255)
                                                                                                                                                                                                                  cGGATGAAGCCGGGCGTTACAGCATGGACGTTGAGTACGGTCAGTACAGCGTTATTCTGTTGGTGGAAGGATTCCCGCCGTCACATGCCGGGACCATTACCGTGTATGAAGATTCTCAACCCGGTACGCTGAATGATTTTCTCGGTGCCATGACGGAGGATGATGCCCGTCCGGAGGCACTGCGCCGTTTTGAACTGATGGTGGAAGAGGTGGCGCGTAACGCGTCCGCGGTGGCACAGAACACGGCAGcc                         >  1:190553/1‑251 (MQ=255)
                                                                                                                                                                                                                  cGGATGAAGACGGGCGTTACAGCATGGATGTGGAGAACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCAACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGACTGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAGGTGCTGCGTCGTCTTGAACTGATGGTGGAAGAGGTGGCGCGTAACGCGTCCGTGGTGGCACAGAGTACGGCAgac                         >  2:176546/1‑249 (MQ=255)
                                                                                                                                                                                                                   ggaggAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAGGTGCTGCGTCGTCTTGAACTGATGGTGGAAGAGGTGGCGCGTAACGCGTCCGTGGTGGCACAGAGTACGGCAgacg                        <  2:276128/247‑4 (MQ=255)
                                                                                                                                                                                                                   ggATGAAGCCGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAGGTGCTGCGTCGTCTTGAACTGATGGTGGAAGAGGTGGCGCGTAACGCGTCCGTGGTGGCACAGAGTACGGCAgacg                        >  1:106096/1‑248 (MQ=255)
                                                                                                                                                                                                                            cGGGCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACTGTTTTACACCATCGCACGCCGGGACCATCACCTTTTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAGGTGCTGCGTCGTCTTGAACTGATGGTGGAAGAGGTGGCGCGTAACGCGTACGTGGTGGCACAGAGTACGGCATACGCgaagaaat               <  1:287257/251‑3 (MQ=255)
                                                                                                                                                                                                                               gCGTTACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAGGTGCTGCGTCGTCTTGAACTGATGGTGGAAGAGGTGGCGCGTAACGCGTCCGTGGTGGCACAGAGTACGGCAGACGCGAAGAAATCAg            <  2:245726/251‑1 (MQ=255)
                                                                                                                                                                                                                                   tACAGCATGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTATGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAGGTGCTGCGTCGTCTTGAACTGATGGTGGAAGAGGTGGCGCGTAACGCGTCCGTGGTGGCACAGAGTACGGCAGACGCGAAGAAATCAGccgg        >  1:203506/1‑249 (MQ=255)
                                                                                                                                                                                                                                         aTGGATGTGGAGTACGGTCAGTACAGTGTCATCCTGCAGGTTGACGGTTTTCCACCATCGCACGCCGGGACCATCACCGTGTCTGAAGATTCACAACCGGGGACGCTGAATGATTTTCTCTGTGCCATGACGGAGGATGATGCCCGGCCGGAGGTGCTGCGTCGTCTTGAACTGATGGTGGAAGAGGTGGCGCGTAACGCGTCCGTGGTGGCACAGAGTACGGCAGACGCGAAGAAATCAGCCGGCGATGc  >  2:31913/1‑251 (MQ=255)
                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                  
TGTCGGTTATAAGTTCTGATTAGCCAGGTAACACAGTGTTATGACAGCCCGCCGGTTCAGGCGGGCTTTTTTGTGGGGTGAATATGGCAGTAAAGATTTCAGGTGTACTGAAAGACGGCACAGGAAAACCGGTACAGAACTGCACAATCCAGCTGAAAGCAAAACGTAACAGCACCACGGTGGTGGTGAACACGCTGGCCTCAGAAAATCCGGATGAAGCCGGGCGTTACAGCATGGACGTTGAGTACGGTCAGTACAGCGTTATTCTGTTGGTGGAAGGATTCCCGCCGTCACATGCCGGGACCATTACCGTGTATGAAGATTCTCAACCCGGTACGCTGAATGATTTTCTCGGTGCCATGACGGAGGATGATGCCCGTCCGGAGGCACTGCGCCGTTTTGAACTGATGGTGGAAGAGGTGGCGCGTAACGCGTCCGCGGTGGCACAGAACACGGCAGCCGCGAAGAAGTCAGCCAGTGATGC  >  NC_000913/1428966‑1429449

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑