Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,636,925	G→C	R77P (CGC→CCC)	creC →	sensory histidine kinase CreC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,636,925	0	G	C	100.0%	90.7 / NA	28	R77P (CGC→CCC)	creC	sensory histidine kinase CreC
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (11/17);  total (11/17)

CCTGTAATGCGTATCGGCATGCGGTTGTTGCTGGGCTATTTTTTACTGGTGGCGGTGGCAGCCTGGTTCGTACTGGCCATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCGCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGACAGGATTATTCGCGCTGGAATGACGTCTGGCTAACGTTGCGTGGTCAGTATGGTGCGCGCAGCACGTTGCAAAATCCTGCCGATCCCGAAAGTTCTGTGATGTATGTTGCCGCACCG  >  NC_000913/4636690‑4637154                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                      ccTGTAATGCGTATCGGCATGCGGTTGTTGCTGGGCTATTTTTTACTGGTGGCGGTGGCAGCCTGGTTCGTACTGGCCATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCa                                                                                                                                                                                                                         >  1:72997/1‑250 (MQ=255)         gCGTATCGGCATGCGGTTGTTGCTGGGCTATTTTTTACTGGTGGCGGTGGCAGCCTGGTTCGTACTGGCCATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGt                                                                                                                                                                                                                 <  1:270749/250‑1 (MQ=255)             aTCGGCATGCGGTTGTTGCTGGGCTATTTTTTACTGGTGGCTGTGGCAGCCTGGTGCGTACTGGCCATTTTTGTCAAAGAAGTTAAACCGGGCGGTCGAAGAGCAACGGAGGGGACGTTGAGCGACACCGCAACGTTTCGGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTTGTGACCCAACGCATGGGCAACTGGCGCAGGGGTGTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCa                                                                                                                                                                                                             <  1:37804/250‑1 (MQ=255)                ggCATGCGGTTGTTGCTGGGCTATTTTTTACTGGTGGCGGTGGCAGCCTGGTTCGTACTGGTCATTGGTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACTTGGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCTGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTGTAATCAGCTACAACAGCCCCCGTTTCGCGCCAATATCGGTGGCATTaa                                                                                                                                                                                                         <  1:303755/251‑1 (MQ=255)                                            tACTGGTGGAGGTGGCAGCCTGGTTCGTGCTGGCCATTTTTGTCAAAGAAGTTAACCCGGGCGTGCGGAGAGCAACGGAGGGGACGTTGATCGACGCCGCAACGTTGCGGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCAAACGCATGGGCAACTGGCGCAGGAGTTTAATCAGCTACACCATCCCCCGTGTCGCGCCAATATCGGTGGCATTAACAAAGTGAGCAATGAATATCATGTCta                                                                                                                                                                              <  2:219163/250‑1 (MQ=255)                                                            aGCCTGGTTCGTACTGGCCATTTTTGTTAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGGGGCAGTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAgg                                                                                                                                                             <  1:186452/251‑1 (MQ=255)                                                                              cATTGTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGATGGGACGTTGATCGACGCCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGAtt                                                                                                                                           <  2:289016/251‑1 (MQ=255)                                                                                                  aaacGGGCGTGCGAAGAGCAACGGAGGGGACGTTGATCGACCCCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCGGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTGTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGa                                                                                                                       <  2:148300/248‑1 (MQ=255)                                                                                                     cGGGCGAGCGAAGAGCAACGGAGGGGACGTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGAc                                                                                                                      >  1:61282/1‑249 (MQ=255)                                                                                                         cGTGCGAAGAGCACCGGAGGGGACGTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGTCCCCACGCATGGGCAACTGGCGCAGGCGTTTCATCATCTACAACCTCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGACTATCATGTCTATATGACCGATGCGCCGGGCACAGTATTGTTCGATTCGGCAAATAAAGCCTTTGGACAgggtt                                                                                                                >  2:72186/1‑248 (MQ=255)                                                                                                            gCGAAGAGCACCGGAGGGGACGTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGACAGGattat                                                                                                              <  1:162395/250‑1 (MQ=255)                                                                                                               aaGAGCAACGGAGGGGACGTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGACAGGATTATTcgc                                                                                                          <  2:258527/251‑1 (MQ=255)                                                                                                                       aggAGGGGACGTTGATCGACACTGAAACGTTGCGGGCGGAGCTGGCGCGGCCCGATTGGCTCTCTGGGGACCCAACGTATGGGCAACTGGCGCAGGAGGTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATAGGGGGCATTAACAAAGTGCGCAATGAATAGCATGTCTATATGTACGATGCGCAGGGCAAAGTAGTGTTCGATTCGGCAAATAAAGCCGTTGGACAGGATTATTCGAGCTGGAAt                                                                                                  <  1:27724/250‑1 (MQ=255)                                                                                                                                   tGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCACGCTTTGCTCTCTGGGGCCCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTGTCGCGCCAATATCGGAGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGAAGGGCAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGACCGGCTTATTCGCGCTGGCATGACGTATGGCt                                                                                       >  2:268725/1‑250 (MQ=255)                                                                                                                                        gACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCGGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCGGCAAATAAATCCGTTGGACAGGATTATTCGCGCTGGAATGACGTCTGGCTAACGtt                                                                                 <  1:263397/251‑1 (MQ=255)                                                                                                                                         acgccGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGACAGGATGATTCGCGCTGGAATGACGTCTGGCTAACGTTg                                                                                <  1:268725/248‑1 (MQ=255)                                                                                                                                           acCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGACAGGATTATTCGCGCTGGAATGACGTCTGGCTAACGTTGCg                                                                              >  1:3513/1‑251 (MQ=255)                                                                                                                                           acCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGACAGGATTATTCGCGCTGGAATGACGTCTGGCTAACGTTGCg                                                                              >  2:191642/1‑251 (MQ=255)                                                                                                                                                                       gTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGACAGGATTATTCGCGCTGGAATGACGTCTGGCTAACGTTGCGTGGTCAGTATGGTGCGCGCAGCACGTTg                                                  >  1:256269/1‑251 (MQ=255)                                                                                                                                                                       gTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGACAGGATTATTCGCGCTGGAATGACGTCTGGCTAACGTTGCGTGGTCAGTATGGTGCGCGCAGCACGTTg                                                  >  1:201651/1‑251 (MQ=255)                                                                                                                                                                            gATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGAATTGTTCGATTCAGCAAATAAAGCCGTTGGACAGGATTATTCGCGCTGGAATGACGTCTGGCTAACGTTGCGTGGTCCGTATGGTGCGGGCAGCACGTTGCaaa                                              >  2:172995/1‑250 (MQ=255)                                                                                                                                                                                        ggggGCCCAACGCATTGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGGTCGATTCGGCAAATAAAGCCGTTGGACAGGATTATGCGCGCTGGAATGACGTCTGGCTAACGTTGCGTGGTCATTAAAGTGCGCGCATCACGTTGCAAAATCCTGCCGATc                                  <  2:280006/250‑1 (MQ=255)                                                                                                                                                                                         gggACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATAATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCGGCAAATAAAGCCGTGGGACAGGATTATTCGCGGGGGAATGACGTCTGGCTAACGTTGCGTGTTCAGTAGGGTGCGCGCAGCAAGTTGCAAAATCCTGCCGATccc                                <  2:15816/251‑1 (MQ=255)                                                                                                                                                                                               cAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGACAGGATTATTCGCGCTGGAATGACGTCTGGCTAACGTTGCGTGGTCAGTATGGTGCGCGCAGCACGTTGCAAAATCCTGCCGATCCCGAAAGt                          <  1:191642/251‑1 (MQ=255)                                                                                                                                                                                                               ggCGCAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGACAGGATTATTCGCGCTGGAATGACGTCTGGCTAACGTTGCGTGGTCAGTATGGTGCGCGCAGCACGTTGCAAAATCCTGCCGATCCCGAAAGTTCTGTGatgtatgt            <  1:115159/249‑1 (MQ=255)                                                                                                                                                                                                                   cAGGCGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGACAGGATTATTCGCGCTGGAATGACGTCTGGCTAACGTTGCGTGGTCAGTATGGTGCGCGCAGCACGTTGCAAAATCCTGCCGATCCCGAAAGTTCTGTGATGTATGTTGCCGc      >  2:254810/1‑251 (MQ=255)                                                                                                                                                                                                                       cGTTTAATCAGCTACAACATCCCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGAGCAGGGCAACGTATTGTTCGATTCGGCAAATAAAGACGTTGGACAGGATTATTCGCGCTGGAATGACGTCTGGCTAACGTTGCGTGGTCAGTATGGTGCGCGCAGCACGGTGCAAAATCCTGCCGATCCCGCAAGTTATGTGATGTATGTTTCCGCACCg  >  2:298998/1‑251 (MQ=255)                                                                                                                                                                                                                       agTTTAATCTGATACACCAGCCCCCTTTTCGCGTCAATATCGGTGGCATTAACAAATGGCTCAATGAATATCAGGTCTATATGACCGATGCGCAGGGCAAAGTATTTTTCGATTCGGCAAATAAAGCCGTTGGACAGGATTATTCGCGCTGGAATGACGTCGGGCTAACGTTGCGTGTGCAGTATGGTGCTCGCAGCACGTTGCAATATCCTGTCTATCCCGAAAGTTCGGTGATGTATGTTGCCGCACCg  <  2:301872/250‑1 (MQ=255)                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                      CCTGTAATGCGTATCGGCATGCGGTTGTTGCTGGGCTATTTTTTACTGGTGGCGGTGGCAGCCTGGTTCGTACTGGCCATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGAGGGGACGTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTGGGGACCCAACGCATGGGCAACTGGCGCAGGCGTTTAATCAGCTACAACATCGCCCGTTTCGCGCCAATATCGGTGGCATTAACAAAGTGCGCAATGAATATCATGTCTATATGACCGATGCGCAGGGCAAAGTATTGTTCGATTCGGCAAATAAAGCCGTTGGACAGGATTATTCGCGCTGGAATGACGTCTGGCTAACGTTGCGTGGTCAGTATGGTGCGCGCAGCACGTTGCAAAATCCTGCCGATCCCGAAAGTTCTGTGATGTATGTTGCCGCACCG  >  NC_000913/4636690‑4637154

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑