Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,604,517	C→T	L120L (CTG→TTG)	bglJ →	DNA‑binding transcriptional regulator BglJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,604,517	0	C	T	100.0%	66.6 / NA	23	L120L (CTG→TTG)	bglJ	DNA‑binding transcriptional regulator BglJ
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (14/9);  total (14/9)

CATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCGCTGGACGGTGTATTAAGTAAAGCGTCAACGCTGGAGATTTTTCATCAGGAACTCTTTTTGTCATTAAATGGTGTACGTCAGGCGACCGACCGACTGAACAATCAGTGGTACATTAACCAAAGCCGGACGTTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGCACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTC  >  NC_000913/4604296‑4604755                                                                                                                                                                                                                              |                                                                                                                                                                                                                                               cATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCGCTGGACGGTGTATTAAGTAAAGCGTCAACGTTGGAGATTTTTCATCAGGAACTCTTTTTg                                                                                                                                                                                                                   <  2:264673/251‑1 (MQ=255)                                              tttctttttctGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCGCTGGACGGTGTATTAAGTAAAGCGTCAACGTTGGAGATTTTTCATCAGGAACTCTTTTTGTCATTAAATGGTGTACGTCAGGCGACCGACCGACTGAACAATCa                                                                                                                                                                       <  1:216248/250‑1 (MQ=255)                                                   ttttctGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCGCTGGACGGTGTATTAAGTAAAGCGTCAACGTTGGAGATTTTTCATCAGGAACTCTTTTTGTCATTAAATGGTGTACGTCAGGCGACCGACCGACTGAACAATCAGTgg                                                                                                                                                                   <  1:224585/249‑1 (MQ=255)                                                    tttctGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCGCTGGACGGTGTATTAAGTAAAGCGTCAACGTTGGAGATTTTTCATCAGGAACTCTTTTTGTCATTAAATGGTGTACGTCAGGCGACCGACCGACTGAACAATCAGTGGTAc                                                                                                                                                                >  2:194058/1‑251 (MQ=255)                                                                                 ggATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCGCTGGACGGTGTATTAAGTAAAGCGTCAACGTTGGAGATTTTTCATCAGGAACTCTTTTTGTCATTAAATGGTGTACGTCAGGCGACCGACCGACTGAACAATCAGTGGTACATTAACCAAAGCCGGACGTTAAGCCCGAc                                                                                                                                   <  1:26502/251‑1 (MQ=255)                                                                                  gATTATCTTGCCTGACTGAACTGGAGATTAAGTTGCCGAGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGGTTGGTTCCTTGTCGCCATCACCGCTGGAAGGTGTATTAAGGAACGCGTCAACGTTGGAGATTTTTCATCAGGAACTCTTTTTGTCATTAAATGGTGTACGTCAGGCGACCGACCGACTGAACAATCAGTGGTACATTAACCCACGCCGGACGTTAAGCCCGACg                                                                                                                                  >  2:137005/1‑251 (MQ=255)                                                                                     tATCTTGCCTGACTGAACTGGCGATTACGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCGCTGGACGGAGTATTAAGTAAAGCGTCAACGTTGGAGATTTTTCATCAGGAACTCTTTTTGTCATTAAATGGTGTACGTCAGGCGACCGACCGACTGAACACTCCGTGGTACATTAACCAACGCCGGACGTTAAGCCCGACgggg                                                                                                                               >  1:199401/1‑249 (MQ=255)                                                                                          tGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGATATTGCGGATGCTGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCGCTGGACGGTGTATTAAGTAAAGCGTCAACGTTGGAGATTTTTCATCAGGAACTCTTTTTGTCATTAAATGGTGTACGTCAGGCGACCGACCGACTGAACAATCAGTGGTACATTAACCAAAGCCGGCCGTTAAGCCCGACGgagagggc                                                                                                                          >  2:265163/1‑248 (MQ=255)                                                                                                                   ttCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCGCTGGACGGTGTATTAAGTAAAGCGTCAACGTTGGAGATTTTTCATCAGGAACTCTTTTTGTCATTAAATGGTGTACGTCAGGCGACCGACCGACTGAACAATCAGTGGTACATTAACCAAAGCCGGACGTTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTgg                                                                                                  <  1:265163/250‑1 (MQ=255)                                                                                                                                gCGTTTAGCTATTGCGGATGATGAGATTGAAGTTCGCCTGCTTGGTTTATTGTCGCCATCAACGCTGGACGGTGCATTAAGTACAGCGTCAACGTTGGAGATTTTTCATCAGGAACTCTTTTTGTCCTTAAATGGTGTACGTCAGGCGACCGACAGACTGAACAATAAGTGGTACATTAACCAAAGCCGGACTTTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACtacatgccac                                                                                    >  2:189508/1‑242 (MQ=255)                                                                                                                                  gTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCGCTGGACGGTGTATTAAGTAAAGCGTCAACGTTGGAGATTTTTCATCAGGAACTCTTTTTGTCATTAAATGGTGTACGTCAGGCGACCGACCGACTGAACAATCAGTGGTACATTAACCAAAGCCGGACGTTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGAcaca                                                                                   >  1:205582/1‑250 (MQ=255)                                                                                                                                        ttATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCGCTGGACGGTGTATTAAGTAAAGCGTCAACGTTGGAGATTTTTCATCAGGAACTCTTTTTGTCATTAAATGGTGTACGTCAGGCGACCGACCGACTGAACAATCAGTGGTACATTAACCAAAGCCGGACGTTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTGcc                                                                            >  1:77862/1‑251 (MQ=255)                                                                                                                                              cGGATGATGAGATTGAAGCTCGGCTGTTTGGTTCATTGTCGCTATCACCGCTGGACGGTGGATTAAGTAAAGCGTCAACGTTGAAGATTTTTCATCAGGAACTCTTTTTGTCATTAAATGGTGTACGTCAGGCGACCGACCGACTGAACAATCAGTGGTACATTAGCCAAAGCCTGACGATAAGCCCGCCGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGATACCCAATGACACAAATTGCCGagcag                                                                      <  2:112631/251‑1 (MQ=255)                                                                                                                                                  tgatgatATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCGCTGGACGGTGTATTAAGTAATGCGTCAACGTTGGAGATTTTTCATCAGGAACTCTTTTTGTCATTAAATGGTGTACGTCAGGCGACCGACCGACTGAACAATCAGTGGTACATTAACCAAAGCCGGACGTTAAGCCCGACGGCGAGAGCAATATTTCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTGCTGCGCAGCtt                                                                   >  2:97812/1‑250 (MQ=255)                                                                                                                                                   gatgatATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCTCCGCTTGACGGTGTATTAAGTAAAGCGTCAACGTTGGAGATGTTTCATCAGGAACTCTTTTTGTCATTAAATGGTGTACGTCAGGCGACCGACCGACTGAACAATCAGTGGTACATTAACCAAAGCCGGACGTTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTa                                                                  <  2:196885/250‑1 (MQ=255)                                                                                                                                                                  cGGCTGATTGGTTCATTGTCGCCATCACCGCTGGACGGTGTATTAAGTAAAGCGTCAACGTTGGAGATTTTTCATCAGGAACTCTTTTTGTCATTAAATGGTGTACGTCAGGCGACCGACCGACTGAACAATCAGTGGTACATTAACCAAAGCCGGACGTTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCaaaa                                                   <  1:194058/250‑1 (MQ=255)                                                                                                                                                                       gATTGGTTCATTGTAGCCATCACCGCTGGACGGTGTATTAAGTAAAGCGTCAACGTTGGAGATTTTTCATCAGGAACTCTTTTTGTCATTAAATGGTGTACGTCAGGCGACCGTCCGACTGAACAATCAGTGGTACATTAACCAAAGCCGGACGTTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTGCAGAGCAGCTTAAACGCAATATCAAAACGATcc                                             >  1:55419/1‑251 (MQ=255)                                                                                                                                                                        aTTGGTTCATTGTCGCCATCACCGCTGGACGGTGTATTAAGTAAAGCGTCAACGTTGGAGATTTTTCATCAGGAACTCTTTTTGTCATTAAATGGTGTACGTCAGGCGACCGACCGACTGAACAATCAGTGGTACATTAACCAAAGCCGGACGTTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAATCGATCCg                                            >  2:78119/1‑251 (MQ=255)                                                                                                                                                                           ggctCATTGTCGCCATCCCCGCTGGACGGTGTATTAAGCAAAGCGTCAACGTTGGAGATTTTTCATCAGGAACTCTTTTTGTCATTAAATGGTGTACGTCAGGCGACCGACCGACTGAACAATCAGTGGCACATTAACCAAAGCCGGACGTTAACCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTACACGCCATATCAAAACGATCCgctc                                         >  2:249991/4‑248 (MQ=255)                                                                                                                                                                                 ttGTCGCCATCACAGCTGGACGGTGTATTAAGTAAAGCGTCAACGTTGGTGGTTTTTCATCAGGGACTCTTTTTGTCATTAAATGGTGTACGTCAGGCGACCGACCGACTGCACAATCAGAGGTACATTCACCAAAGCCGGACGTTAAGCCCGACGGAGAGAGAACTATTGCGCTTAATGACGCGTGGCTACACAATGACACAAATTGGCGAGCAGATAAAAGGCAATCTCAAAACGaaccataaccata                                    >  2:296468/1‑238 (MQ=255)                                                                                                                                                                                     cGCCATCACCGCTGGACGGTGTATTAAGTAAAGCGTCAACGTTGGAGATTTTTCATCAGGAACTCTTTTTGTCATTAAATGGTGTACGTCAGGCGACCGACCGACTGAACAATCAGTGGTACATTAACCAAAGCCGGACGTTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGCACATAAAttt                               >  1:227864/1‑251 (MQ=255)                                                                                                                                                                                                             tAAGTAAAGCGTCAACGTTGGAGATTTTTCATCAGGAACTCTTTTTGTCATTAAATGGTGTACGTCAGGCGACCGACCGACTGAACAATCAGTGGTACATTAACCAAAGCCGGACGTTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGACCAGTGCACATAAATTTAATGTGATGTCGAAAATGGGCGTc       >  2:271112/1‑251 (MQ=255)                                                                                                                                                                                                                  aaaGGGTCAACGTTGGAGATTTTTCAGCAGGAACTCTTTTTGTCATTAAATGGTGTTCGGCAGGCGACCGACCGACTGAACAATCAGTGGTACATTAACCAAAGCCGGACGTTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGCACATAAATTTAATGTGATGGCGAAACTGGGCGGCAGTTc  <  2:190063/251‑1 (MQ=255)                                                                                                                                                                                                                              |                                                                                                                                                                                                                                               CATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCGCTGGACGGTGTATTAAGTAAAGCGTCAACGCTGGAGATTTTTCATCAGGAACTCTTTTTGTCATTAAATGGTGTACGTCAGGCGACCGACCGACTGAACAATCAGTGGTACATTAACCAAAGCCGGACGTTAAGCCCGACGGAGAGAGAAATATTGCGCTTTATGTCGCGTGGCTACTCAATGACACAAATTGCCGAGCAGCTTAAACGCAATATCAAAACGATCCGTGCACATAAATTTAATGTGATGTCGAAACTGGGCGTCAGTTC  >  NC_000913/4604296‑4604755

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑