Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,587,365	C→T	S139S (AGC→AGT)	mrr →	Type IV methyl‑directed restriction enzyme EcoKMrr

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,587,365	0	C	T	93.1%	74.1 / NA	29	S139S (AGC→AGT)	mrr	Type IV methyl‑directed restriction enzyme EcoKMrr
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (7/20);  minor base G (0/2);  total (7/22)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGGCGCATGACCGTTTAAAACGTGCCGGGTTGTCGCAAAGTTTGTCGCGTGGCAAATGGTGCCTGACTCCTGCGGGTTTTGACTGGGTTGCGTCTCATCCCCAGCCAATGACGGAGCAGGAGACGAACCATCTGGCCTTCGCTTTTGTGAATGTCAAACTTAAGTCACGGCCGGATGCCGTCGATTTAGATCCGAAAGCCGACTCTCCCGATCATGAAGAACTTGCAAAGAGCAGCCCGGACGATCGGTTAGATCAGGCGCTAAAAGAGCTTCGTGATGCGGTGGCTGATGAGGTTCTGGAAAACTTATTGCAGGTTTCTCCTTCGCGCTTTGAAGTCATTGTTCTGGATGTTTTGCATCGCCTGGGGTATGGCGGCCACCGTGATGATTTGCAGCGTGTTGGCGGTACTGGAGATGGTGGCATCGATGGTGTGATATCGCTTGATAAACTTGGCCTGGAGAAAGTTTAT  >  NC_000913/4587130‑4587599                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                           ggtctcATGACCGTTTAAATCGTGACGGGTTTTTGCAAAGTTTGTCGCGTGGCAAATGGTGACGGACTCCGGCGGGTTTTGACTGGGTTGCGTCTCATCCCCAGCCAATGACGGAGCAGGAGACGAACCATCTGGCCTTCACTTTTGTGAATGTCAAACTTAAGTCACGGCCGGATGCCGTCGATTGAGATCCGAAAGCCGACTCTCCCGATCATGAAGAACTTGCAAAGAGCAGTCCGGACGATCGGtt                                                                                                                                                                                                                              <  2:164636/245‑1 (MQ=255) gggCGCATGACCGTTTAAAACGTGCCGGGTTGTCGCAAAGTTTGTCGCGTGGCAAATGGTGCCTGACTCCTGCGGGTTTTGACTGGGTTGCGTCTCATCCCCAGCCAATGACGGAGCAGGAGACGAACCATCTGGCCTTCGCTTTTGTGAATGTCAAACTTAAGTCACGGCCGGATGCCGTCGATTTAGATCCGAAAGCCGACTCTCCCGATCATGAAGAACTTGCAAAGAGCAGTCCGGACGATCGGt                                                                                                                                                                                                                               <  1:189769/249‑1 (MQ=255)  ggCGCATGACCGTTTAAAACGTGCCGGGTTGTCGCAAAGTTTGTCGCGTGGCAAATGGTGCCTGACTCCTGCGGGTTTTGACTGGGTTGCGTCTCATCCCCAGCCAATGACGGAGCAGGAGACGAACCATCTGGCCTTCGCTTTTGTGAATGTCAAACTTAAGTCACGGCCGGATGCCGTCGATTTAGATCCGAAAGCCGACTCTCCCGATCATGATGAACTTGCAAAGAGCAGTCAGGACGATCGGtt                                                                                                                                                                                                                              >  2:158545/1‑249 (MQ=255)         gACCGTTTAAAACGTGCCGGGTTGTCGCAAAGTTTGTCGCGTGGCAAATGGTGCCTGACTCCTGCGGGTTTTGACTGGGTTGCGTCTCATCCCCAGCCAATGACGGAGCAGGAGACGAACCATCTGGCCTTCGCTTTTGTGAATGTCAAACTTAAGTCACGGCCGGATGCCGTCGATTTAGATCCGAAAGCCGACTCTCCCGATCATGAAGAACTTGCAAAGAGCAGTCCGGACGATCGGTTAGATCAGgc                                                                                                                                                                                                                     >  2:2937/1‑251 (MQ=255)                tAAAACGTGCCGGGTTGGCGCAAAGTTTGTCGCGTGGCAAATGGTGCCTGACTCCTGCGGGTTTTGACTGTGTTGCGTCTCATCCCCAGCCAATGACGGAGCAGGAGACGAACCATCTGGCCTTCGCTTTTGTGAATGTCAAACTTAAGTCACGGCCGGATGCCGTCGATTTAGATCCGAAAGCCGACTCTCCCGATCATGAAGAACTTGCAAAGAGCAGTCCGGACGATCGGTTAGATCAGGCGCTaaa                                                                                                                                                                                                               <  1:19116/250‑1 (MQ=255)                                            gTCGCGTGGCAAATGGTGCCTGACTCCTGCGGGTTTGGACTGGGTTGCGTCTCATCCCCAGCCAATGACGGAGCAGGAGACGAACCATCTGGCCTTCGCTTTTGTGAATGTCAAACTTAAGTCACGGCCGGATGCCGTCGATTTAGATCCGAAAGCCGACTCTCCCGATCATGAAGAACTTGCAAAGAGCAGTCCGGACGATCGGTTAGATCAGGCGCTAAAAGAGCTTCGTGATGCGGTGGCTGATGAg                                                                                                                                                                                   <  1:209247/250‑1 (MQ=255)                                                    gCAAATGGTGCCTGACTCCTGCGGGTTTTGACTGGGTTGCGTCTCATCCCCAGCCAATGACGGAGCAGGAGACGAACCATCTGGCCTTCGCTTTTGTGAATGTCAAACTTAAGTCACGGCCGGATGCCGTCGATTTAGATCCGAAAGCCGACTCTCCCGATCATGAAGAACTTGCAAAGAGCAGTCCGGACGATCGGTTAGATCAGGCGCTAAAAGAGCTTCGTGATGCGGTGGCTGATGAGGTTCTGGaa                                                                                                                                                                          <  2:101555/251‑1 (MQ=255)                                                           gTGCCTGACTCCTGCGGGTTTTGAGTGGGTTGCGTCTCATTCCCAGCCAATGACGGAGCAGGAGACGAACCATCTGGCCTTCGCTTTTGTGAATGTCAAACTTAAGTCACGGCCGGATGCCGTCGATTTAGATCCGAAAGCCGACTCTCCCGATCATGAAGAACTTGCAAAGAGCAGTCCGGACGATCGGTTAGATCAGGCGCTAAAAGAGCTTCGTGATGCGGTGGCTGATGAGGTTCTGGAAAACTTAt                                                                                                                                                                   <  1:1599/251‑1 (MQ=255)                                                                 gACTCCTGCGGGTTTTGACTGGGTTGCGTCTCATCCCCAGCCAATGACGGAGCAGGAGACGAACCATCTGGCCTTCGCTTTTGTGAATGTCAAACTTAAGTCACGGCCGGATGCCGTCGATTTAGATCCGAAAGCCGACTCTCCCGATCATGAAGAACTTGCAAAGAGCAGTCCGGACGATCGGTTAGATCAGGCGCTAAAAGAGCTTCGTGATGCGGTGGCTGATGAGGTTCTGGAAAACTTATTGCAgg                                                                                                                                                             <  1:93599/251‑1 (MQ=255)                                                                      cggCGGGTTGTGACTGGTTTGCGTCTCATCCCCAGCCAATGACGGAGCAGGAGACGAACCATCTGGCCTTCGCTTTTGTGAATGGCAAACTTAAGTCACGGCCGGATGCCGTCGATTTAGATCCGAAAGCCGACTCTCCCGATCATGAAGAACTTGCAAAGAGCATTCCGGACGATCGGTTAGATCACGCGCTAAAAGATCTTCCTGATGCGGTGGCTGATGAGGTGCTGGAAAACTTATTGCAGGttgct                                                                                                                                                        <  1:198804/249‑4 (MQ=255)                                                                         cGGGTTTTGACTGGGTTGCGTCTCATCCCCAGCCAATGACGGAGCAGGAGACGAACCATCTGGCCTTCGCTTTTGTGAATGTCAAACTTAAGTCACGGCCGGATGCCGTCGATTTAGATCCGAAAGCCGACTCTCCCGATCATGAAGAACTTGCAAAGAGCAGTCCGGACGATCGGTTAGATCAGGCGCTAAAAGAGCTGCGTGATGCGGTGGCTGATGAGGTTCTGGAAAACTTATTGCAGGTTTCTCCt                                                                                                                                                     <  1:178609/251‑1 (MQ=255)                                                                               ttGACTGTGTGGCGGCTCATCCCCAGCCGATGACGGAGCAGGAGACGAACCAGCTGGCCTTCGCTTTTGTGAATGTCAAACTTAAGTCACGGCCGGATGCCGTCGATTTAGATCCGAAAGCCGACTCTCCCGATCATGAAGAACTTGCAAAGAGCAGTCCGGACGATCGGTTAGATCAGGCTCTAAAAGAGCTTCGTGATGCGGTGGCTGATGAGGTTCTGGAAAACTTATTGCAGGTTTCTCCTTcgcg                                                                                                                                                <  1:185739/250‑1 (MQ=255)                                                                                     gggTTGCGTCTCATCCCCAGCCAATGACGGAGCAGGAGACGAACCATCTGGCCTTCGCTTTTGTGAATGTCAAACTTAAGTCACGGCCGGATGCCGTCGATTTAGATCCGAAAGCCGACTCTCCCGATCATGAAGAACTTGCAAAGAGCAGGCCGGACGATCGGTTAGATCAGGCGCTAAAAGAGCTTCGTGATGCGGTGGCTGATGAGGTTCTGGAAAACTTATTGCAGGTTTCTCCTTCGCGCTTTGaa                                                                                                                                         <  2:288409/251‑1 (MQ=255)                                                                                                cATCCCCAGCCAATGACGGAGCAGGAGACGAACCATCTGGCCTTCGCTTTTGTGAATGTCAAACTTAAGTCACGGCCGGATGCCGTCGATTTAGATCCGAAAGCCGACTCTCCCGATCATGAAGAACTTGCAAAGAGCAGTCCGGACGATCGGTTAGATCAGGCGCTAAAAGAGCTTCGTGATGCGGTGGCTGATGAGGTTCTGGAAAACTTATTGCAGGTTTCTCCTTCGCGCTTTGAAGTCATTGt                                                                                                                                 <  1:245878/248‑1 (MQ=255)                                                                                                cATCCCCAGACAATGACGGAGCAGGAGACGAACCATCTGGCCTTCGCTTTTGGGAATGGCAAACTTAAGTCACGGCCGGATGCCGTCGAGTTAGATCCGAAAGCCGACTCTCCCGATCATGAAGAACTTGCAAAGAGCAGTCCGGACGATCGGTTAGATCAGGCGCTAAAAGAGCTTCGTGATGCGGTGGCTGATGAGGTTCTGGAAAACTTATTGCAGGTTTCTCCTTCGCGCTTTGAAGTCATTGTTCt                                                                                                                              <  2:286062/251‑1 (MQ=255)                                                                                                   ccccAGCCAATGACGGAGCAGGAGACGAACCATCTGGCCTTCGCTTTTTTGAATGTCAAACTTAAGTCACGGCCGGATGCCGTCGATTTAGATCCGAAAGCCGACTCTCCCGATCATGAAGAACTTGCAAAGAGCAGTCCGGACGATCGGTTAGATCAGGCGCTAAAAGAGCTTCGTGATGCGGTGGCTGATGAGGTTCTGGAAAACTTATTGCAGGTTTCTCCTTCGCGCTTTGAAGTCATTGTTCTg                                                                                                                             <  2:172712/249‑1 (MQ=255)                                                                                                        gCCAATGACGGAGCAGGAGACGAACCATCTGGCCTTCGCTTTTGTGAATGTCAAACTTAAGTCACGGCCGGATGCCGTCGATTTAGATCCGAAAGCCGACTCTCCCGATCATGAAGAACTTGCAAAGAGCAGTCCGGACGATCGGTTAGATCAGGCGCTAAAAGAGCTTCGTGATGCGGTGGCTGATGAGGTTCTGGAAAACTTATTGCAGGTTTCTCCTTCGCGCTTTGAAGTc                                                                                                                                      >  1:294233/1‑235 (MQ=255)                                                                                                        gCCAATGACGGAGCAGGAGACGAACCATCTGGCCTTCGCTTTTGTGAATGTCAAACTTAAGTCACGGCCGGATGCCGTCGATTTAGATCCGAAAGCCGACTCTCCCGATCATGAAGAACTTGCAAAGAGCAGTCCGGACGATCGGTTAGATCAGGCGCTAAAAGAGCTTCGTGATGCGGTGGCTGATGAGGTTCTGGAAAACTTATTGCAGGTTTCTCCTTCGCGCTTTGAAGTc                                                                                                                                      <  2:294233/235‑1 (MQ=255)                                                                                                                 ggAGCAGGAGACGAACCATCTGGCCTTCGCTTTTGTGAATGTCAAACTTAAGTCACGGCCGGATGCCGTCGATTTAGTTCCGAAAGCCGACTCTCCCGATCATGACGAACTTGCAAAGAGCCGTCCGGACGATCGGTTAGATCAGGCGCTACAAGAGCTTCGTGATGCGGTGGCt                                                                                                                                                                                         >  2:15599/1‑175 (MQ=255)                                                                                                                 ggAGCAGGAGACGAACCATCTGGCCTTCGCTTTTGTGAATGTCAAACTTAAGTCACGGCCGGATGCCGTCGATTTAGATCCGAAAGCCGACTCTCCCGATCATGAAGAACTTGCAAAGAGCAGTCCGGACGATCGGTTAGATCAGGCGCTAAAAGAGCTTCGTGATGCGGTGGCt                                                                                                                                                                                         <  1:15599/175‑1 (MQ=255)                                                                                                                              aaCCATCTGGCCTTCGCTTTTGTGAATGTCAAACTTAAGTCACGGCCGGATGCCGTCGATTTAGATCCGAAAGCCGACTCTCCCGATCATGAAGAACTTGCAAAGAGCAGTCCGGACGATCGGTTAGATCCGGCGCTAAAAGAGCTTCGTGATGCGGTGGCTGATGAGGTTCTGGAAAACTTATTGCAGGTTTCTCCTTCGCGCTTTGAAGTCATTGTTCTGGATGTTTTGCATCGACTGGGGTATgtagg                                                                                                >  2:243195/1‑247 (MQ=255)                                                                                                                                 cATCTGGCCTTCGCTTTTGTGAATGTCAAACTTAAGTCACGGCCGGCTGCCGTCGATTTAGATCCGAAAGCCGACTCTCCCGATCATGAAGAACTTGCAAAGAGCAGTCCGGACGATCGGTTAGATCAGGCGCTAAAAGAGCTTCGTGATGCGGTGGCTGATGAGGTTCTGGAAAACTTATTGCAgg                                                                                                                                                             >  1:280255/1‑187 (MQ=255)                                                                                                                                 cATCTGGCCTTCGCTTTTGTGAATGTCAAACTTAAGTCACGGCCGGATGCCGTCGATTTAGATCCGAAAGCCGACTCTCCCGATCATGAAGAACTTGCAAAGAGCAGTCCGGACGATCGGTTAGATCAGGCGCTAAAAGAGCTTCGTGAGGCGGTGGCTGATGAGGTTCTGGAAAACTTATTGCAgg                                                                                                                                                             <  2:280255/187‑1 (MQ=255)                                                                                                                                                       aTGTCAAACTTAAGTCACGGCCGGATGCCGTCGATTTAGATCCGAAAGCCGACTCTCCCGATCATGAAGAACTTGCAAAGAGCAGTCCGGACGATCGGTTAGATCAGGCGCTAAAAGAGCTTCGTGATGCGGTGGCTGATGAGGTTCTGGAAAACTTATTGCAGGTTTCTCCTTCGCGCTTTGAAGTCATTGTTCTGGATGTTTTGCATCGCCTGGGGTATGGCGGCCACCGTGATGATTTGCAGCGTGtt                                                                       <  2:219444/251‑1 (MQ=255)                                                                                                                                                        tGTCAAACTTAAGTCACGGCCGGATGCCGTCGATTTAGATCCGAAAGCCGACTCTCCCGATCATGAAGAACTTGCAAAGAGCAGTCCGGACGATCGGTTAGATCAGGCGCTAAAAGAGCTTCGTGATGCGGTGGCtga                                                                                                                                                                                       >  1:222073/1‑138 (MQ=255)                                                                                                                                                        tGTCAAACTTAAGGCACGGCCGGATGCCGTCTATTTAGATCCGAAAGCCGACTCTCCCGATCATGAAGAACTTGCAAAGAGCAGTCCGGACGATCGGTTAGATCAGGCGCTAAAAGAGCTTCGTGATGCGGTGGCtga                                                                                                                                                                                       <  2:222073/138‑1 (MQ=255)                                                                                                                                                                                      cgattcagagtcGAAAGCCGACTGCCCCGAACATGAAGAACTTGCAAAGAGCAGTCCGGACGATCGGTTAGAGCAGGCGCTAAAAGAGCTTCGTGATGCGGTGGCTGAGGAGGGTCTGGTAACCTTATTGCATGTTTCTCCTTCGCGTTTTGAAGTGTTTGGTCTTGATGTGTTGGATCGTTTTGGGTATGGCGGCCACCTTGATGATTTGGCGCTTGTCTGCGGTACTGGAGATGGGGGCATCTATGgt                                         <  2:288256/239‑1 (MQ=255)                                                                                                                                                                                                    aaGCCGACTCTCCCGATCATGAAGAACTTGCAAAGAGCAGTCCGGACGATCGGTTAGATCAGGCGCTAAAAGAGCTTCGTGATGCGGTGGCTGATGAGGTTCTGGAAAACTTATTGCAGGTTTCTCCTTCGCGCTTTGAAGTCATTGTTCTGGATGTTTTGCATCGCCTGGGGTATGGCGGCCACCGTGATGATTTGCAGCGTGTTGGCGGTACTGGAGATGGTGGCATCGATGGTGTGATATCGCTTGAt                          <  1:2937/251‑1 (MQ=255)                                                                                                                                                                                                                            aaCTTGCAAAGAGCAGGCCGGACGATCGGTTAGAGCAGGCGCTAAAAGAGCTTCGTGATGCGGTGGCTGATGAGGTTCTGGAAAACTTATTGCAGGTTTCTCCGTCGCGCTTTGAAGTCATTGTTCTGGATGTGTTGCATCGCCTGGTGTATGGCGGCCACCGTGATGATTTGCAGCGTGTTGGCGGTACTGGAGAGTGTGGCATCGATGGTGTGATATCGCTTGATAAACTTGTCCTGGAGAAATTTTAt  <  2:257231/251‑1 (MQ=255)                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                           GGGCGCATGACCGTTTAAAACGTGCCGGGTTGTCGCAAAGTTTGTCGCGTGGCAAATGGTGCCTGACTCCTGCGGGTTTTGACTGGGTTGCGTCTCATCCCCAGCCAATGACGGAGCAGGAGACGAACCATCTGGCCTTCGCTTTTGTGAATGTCAAACTTAAGTCACGGCCGGATGCCGTCGATTTAGATCCGAAAGCCGACTCTCCCGATCATGAAGAACTTGCAAAGAGCAGCCCGGACGATCGGTTAGATCAGGCGCTAAAAGAGCTTCGTGATGCGGTGGCTGATGAGGTTCTGGAAAACTTATTGCAGGTTTCTCCTTCGCGCTTTGAAGTCATTGTTCTGGATGTTTTGCATCGCCTGGGGTATGGCGGCCACCGTGATGATTTGCAGCGTGTTGGCGGTACTGGAGATGGTGGCATCGATGGTGTGATATCGCTTGATAAACTTGGCCTGGAGAAAGTTTAT  >  NC_000913/4587130‑4587599

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑