Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,571,280 C→T E99K (GAG→AAG)  yjiR ← fused putative DNA‑binding transcriptional regulator/putative aminotransferase YjiR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,571,2800CT96.2% 81.0 / NA 26E99K (GAG→AAG) yjiRfused putative DNA‑binding transcriptional regulator/putative aminotransferase YjiR
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (10/15);  minor base G (0/1);  total (10/16)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TCGAGTTATGGCAGCCGCTGGTGATGATGATGTCATCGGCGGTGACCACCGAGCCGCTGTCGAGCATCAGGCGGGCAATCTGCTCTCGCAATACTCGCTGACCGGCTAACAAGTCATAACCGAGAACGGTTTGCAGATTATGCTGCACCACCCGGCTTAGCTCCCGCCACAGCGG‑TTTCAGGCTGGGCGCTTCGACATCCGGTGTGCTTTTGCTTAACGG‑AACAATGGAACTGTCGCTATGCGCCTCCAGCATATCCAGCACCTGATCCCACTGGGTAATTTCCACCGGGCGCTGCACCGGACGCGTCATCGGCGGTACTGGCGGCTGGGCTTTACGTTGTGCG‑ACAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTGCTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAATTTCTCCCCGTGACGATACAGCCCTT  >  NC_000913/4571034‑4571525
                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                      
ccgtgttagggcggccgcgggggttgatgatgTCATCGGCGGTGCCCACCGAGCCGCGGTCGAGCATCAGGCGGCAAATCTGCTCTCGCAATACTCGCTGTCCGGCTAACAAGTCATACCCGAGATCGGTTTGCAGATTATGCTGCCCCCCCCGGCTTAGCTCCCGCCACAGCGG‑TTTCAGGCTGGGCGCTTCGACATCCGGTGTGCTTTTGCTTAACGG‑AACAATGGAACTGTCGCTATGCTCCTTCAGc                                                                                                                                                                                                                                                    <  2:288279/227‑1 (MQ=255)
 cgagtgagggcggtcGCTGGTGATGATTATGTCATCGTCGGTGACCACCGAGCCGCTGTCGAGCAGCAGGCGGGCAATCTGCTCTCGCAATACTCGCTGACCGGCTAACAAGTCATAACCGTGACCGGTTTGCAGATTATGCTGCACCCCCCGGCTTGGCTCCCGCCACAGCGG‑TTTCAGGCTGGGCGCGTCGACATCCGGTGTGCTTTTGCTTAACGG‑AACAATGGAACGGTCGCTATGCGCCTTCAGCa                                                                                                                                                                                                                                                   <  2:180866/237‑1 (MQ=255)
     ttATGGCAGCCGCTGGTGATGATGATGTCATCGGCGGTGACCACCGCGGCGCAGTCGAGGATCAGGAGGGGAACCTGCACCAACAATACTCGCAGAGCGGCGAAAAAGACATAACCCGGAACGGTTTGCAGACTATTGTGCCCCAAACGGCTTAGCCCCCGCCACCGCGGGTTTC‑GGCTGGGCGCCTTGTCATTCGCTGTTCTTTTTCTTGACGGGAACAA‑GGAAACGTCGCTATGCGCCCTCAGCATATc                                                                                                                                                                                                                                               >  1:292863/1‑251 (MQ=255)
           cAGCCGCGGGTGTTGAGAATGTCCTCGGCGGTACCCCCCGCGCCGCTGTCGAGCATCAGGCGGGCAATCTGCTCTCGCAATACTCGCTGCCCGTCTAACAAGTCATAACCGAGACCGGTTTGCAGATTATGCTGCCCCCCCCGGCTTAGCTCCCGCCACAGCGG‑TTTCAGGCTGGGCGCTTCGACATCCGGTGTGCTTTTGCTTAACGG‑AACAATGGAACTGTCGCTATGCGCCTTCAGCATATCCAGCAc                                                                                                                                                                                                                                         <  1:64410/251‑1 (MQ=255)
                               gTCATCGGCGGTGCCCACCGAGCCGCTGTCGAGCATCAGGCGGGCAATCTGCTCTCGCAATACTCGCTGACCGGCTAACAAGTCATAACCGAGAACGGTTTGCAGATTATGCTGCCCCACCCGGCTTAGCTCCCGCCACAGCGG‑TTTCAGGCTGGGCGCTTCGACATCCGGTGTGCTTTTGCTTAACGG‑AACAATGGAACTGTCGCTATGCGCCTTCAGCATATCCAGCACCTGATCCCACTGGGTAAttt                                                                                                                                                                                                                     <  1:106635/251‑1 (MQ=255)
                                       cggTGCCCACCGGGCCGCTGCCGAGCATCAGGCGGGCAATCTGCTCTCGCAAGACTCGCTGACCGGCTAACAAGTCATAACCGAGAACGGTTTGCAGATTATGCGGCACCACCCGGCTTAGCTCCCGCCACAGCGG‑TTTCAGGCTGGGCGCTTCGACATCCGGTGTGCTTGTGCTTAACGG‑AACAATGGAACTGTCGCTATGCGCCTTCAGCATATCCAGCACCTGATCCCACTGGGTAATTTCCACCggg                                                                                                                                                                                                             <  1:191154/251‑1 (MQ=255)
                                         gtgccccccacgCCGCTGCCGAGCATCAGGCGGGCAATCTGCTCTCTCAAAACTCGCTGCCCGGCTAACAAGTCATAACCGAGAACGGTTTGCAGATTATGCGGCACCACCCGGCTTAGCTCCCGCCACAGCGG‑TTTCAGGCTGGGCGCTTCGACATCCGGTGTGCTTTTGCTTAACGG‑AACAATGGAACTGTCGCTATGCGCCTTCAGCATATCCAGCACCTGATCCCACTGGGTAATTTCCACCGGgcg                                                                                                                                                                                                           <  2:268275/240‑1 (MQ=255)
                                         gTGACCACCGAGCCGCTGTCGAGCATCAGGCGGGCAATCTGCTCTCGCAATACTCGCTGACCGGCTAACAAGTCATAACCGAGAACGGTTTGCAGATTATGCTGCACCACCCGGCTTAGCTCCCGCCACAGCGG‑TTTCAGGCTGGGCGCTTCGACATCCGGTGTGCTTTTGCTTAACGG‑AACAATGGAACTGTCGCTATGCGCCTTCAGCATATCCAGCACCTGATCCCACTGGGTAATTTCCACCGGgcg                                                                                                                                                                                                           >  1:44143/1‑251 (MQ=255)
                                                            gtaGCATCAGGCGGGCAATCTGCTCTCGCAATACTCGCTGACCGGCTAACAAGTCATAACCGAGAACGGTTTGCAGATTATGCTGCACCACCCGGCTTAGCTCCCGCCACAGCGG‑TTTCAGGCTGGGCGCTTCGACATCCGGTGTGCTTTTGCTTAACGG‑AACAATGGAACTGTCGCTATGCGCCTTCAGCATATCCAGCACCTGATCCCACTGGGTAATTTCCACCGGGCGCTGCACCGGACGCGTCATc                                                                                                                                                                                        <  1:164713/249‑1 (MQ=255)
                                                             gAGCATCAGGCGGGCAATCTGCTCTCGCAATACTCGCTGACCGGCTAACAAGTCATAACCGAGAACGGTTTGCAGATTATGCTGCACCACCCGGCTTAGCTCCCGCCACAGCGG‑TTTCAGGCTGGGCGCTTCGACATCCGGTGTGCTTTTGCTTAACGG‑AACAATGGAACTGTCGCTATGCGCCTTCAGCATATCCAGCACCTGATCCCACTGGGTAATTTCCACCGGTCGCTGCACCGGCCGCGTCATcg                                                                                                                                                                                       >  2:167716/1‑251 (MQ=255)
                                                                    aGGCGGGCAATCTGCTCTCGCAATACTCGCTGACCGGCTAACTAGTCATAACCGAGAACGGTTTGCAGATTATGCTGCACCACCCGGCTTAGCTCCCGCCACAGCGG‑TTTCAGGCTGGGCGCTTCGACATCCGGTGTGCTTTTGCTTAACGG‑AACAATGGAACTGTCGCTATGCGCCTTCAGCATATCCAGCACCTGATCCCACTGGGTAATTTCCACCGGGCGCTGCACCGGACGCGTCATCGGCGGTAc                                                                                                                                                                                >  1:73205/1‑251 (MQ=255)
                                                                      gCGGGCAATCTGCTCGCGCAATACTCGCTGACCGGCTAACAAGTCATACCCGAGAACGGTTTGCAGATTAGGCTGCCCCACCCGGCTTAGCTCCCGCCACAGCGG‑TTTCAGGCTGGGCGCTTCGACATCCGGTGTGCTTTTGCTTAACGG‑AACAATGGAACTGTCGCTATGCGCCTTCAGCATATCCAGCACCTGATCCCACTGGGTAATTTCCACCGGGCGCTGCACCGGACGCGTCATCGGCGGTACTg                                                                                                                                                                              <  1:30588/251‑1 (MQ=255)
                                                                       cGGGCAATCTGCTCTCGCAATACTCGCTGACCGGCTAACAAGTCATAACCGAGAACGGTTTGCAGATTATGCTGCACCACCCGGCTTAGCTCCCGCCACAGCGG‑TTTCAGGCTGGGCGCTTCGACATCCGGTGTGCTTTTGCTTAACGG‑AACAATGGAACTGTCGCTATGCGCCTTCAGCATATCCAGCACCTGATCCCACTGGGTAATTTCCACCGGGCGCTGCACCGGACGCGTCATCGGCGGTACTgg                                                                                                                                                                             >  2:95013/1‑251 (MQ=255)
                                                                             aTCTGCTCTCGCAATACTCGCTGACCGGCTAACAAGTCATAACCGAGAACGGTTTGCAGATTATGCTGCACCACCCGGCTTAGCTCCCGCCACAGCGG‑TTTCAGGCTGGGCGCTTCGACATCCGGTGTGCTTTTGCTTAACGG‑AACAATGGAACTGTCGCTATGCGCCTTCAGCATATCCAGCACCTGATCCCACTGGGTAATTTCCACCGGGCGCTGCACCGGACGCGTCATCGGCGGCACAggcggcgg                                                                                                                                                                       >  1:294879/1‑249 (MQ=255)
                                                                                       gCAATACTCGCTGACCGGCTAACAAGTCATAACCGAGAACGGTTTGCAGATTATGCTGCCCCCCCCGGCTTAGCCCCCGCCACAGCGG‑TTTCAGGCTGGGCGCTTCGACATCCGGTGTGCTTTTGCTTAACGG‑AACAATGGAACTGTCGCTATGCGCCTTCAGCATATCCAGCACCTGATCCCACTGGGTAATTTCCACCGGGCGCTGCACCGGACGCGTCATCGGCGGTACTGGCGGCTGGGCTTTACGt                                                                                                                                                             <  1:95013/251‑1 (MQ=255)
                                                                                        cAATACTCGCTGACCGGCTAACAAGTCATAACCGAGAACGGTTTGCAGATTATGCTGCACCACCCGGCTTAGCTCCCGCCACAGCGG‑TTTCAGGCTGGGCGCTTCGACATCCGGTGTGCTTTTGCTTAACGG‑AACAATGGAACTGTCGCTATGCGCCTTCAGCATATCCAGCACCTGATCCCACTGGGTAATTTCCACCGGGCGCTGCACCGGACGCGTCATCGGCGGTACTGGCGGCTGGGGttttagtt                                                                                                                                                            >  1:93717/1‑246 (MQ=255)
                                                                                                                              cGGTTTGCAGATTATGCTGCACCCCCCGGCTTAGCTCCCGCCACAGCGG‑TTTCAGGCTGGGCGCTTCGACATCCGGTGTGCTTTTGCTTAACGG‑AACAATGGAACTGTCGCTATGCGCCTTCAGCATATCCAGCACCTGATCCCACTGGGTAATTTCCACCGGGCGCTGCACCGGACGCGTCATCGGCGGTACTGGCGGCTGGGCTTTACGTTGTGCG‑ACAAAATAACCCGAACGCGGCTGCGGAGtgatg                                                                                                                     <  1:219435/251‑1 (MQ=255)
                                                                                                                                        aTTATGCGGCCCCCCCCGGCTTACCCCCCGCCACAGCGG‑TTTCAGGCTGGGCGCTTCGACATCCGGTGTGCTTTGGCTTAACGG‑AACAATGGAACGGTCGCTATGCGCCTTCAGCATATCCAGCACCTGATCCCACTGGGTAATTTCCACCGGGCGCTGCACCGGACGCGTCATCGGCGGTACTGGCGGCTGGGCTTTACGTTGTGCG‑ACAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCg                                                                                                           <  2:249985/251‑1 (MQ=255)
                                                                                                                                                gcACCCCCCGGCTTGGCTCCCGCCACAGCGG‑TTTCAGGCTGGGCGCTTCGACATCCGGTGTGCTTTTGCTTAACGG‑AACAATGGAACTGTCGCTATGCGCCTTCAGCATATCCAGCACCTGATCCCACTGGGTAATTTCCACCGGGCGCTGCACCGGACGCGTCATCGGCGGTACTGGCGGCTGGGCTTTACGTTGTGCG‑ACAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCa                                                                                                     <  2:73205/249‑1 (MQ=255)
                                                                                                                                                                                      gCTGGGCGCTTCGACATCCGGTGTGCTTTTGCTTAACGG‑AACAATGGAACTGTCGCTATGCGCCTTCAGCATATCCAGCACCTGATCCCACTGGGTAATTTCCACCGGGCGCTGCACCGGACGCGTCATCGGCGGTACTGGCGGCTGGGCTTTACGTTGTGCG‑ACAAAAAAAACCGAACGCGGCTGCGGGGTGGTGGGGTTTATCGGCTCCACCGTCTGGAACGCctgcggccaggggcgggtaatggagc                                                              >  1:11687/1‑229 (MQ=255)
                                                                                                                                                                                                  gACATCCGGTGTGCTTTTGCTTAACGG‑AACAATGGAACTGTCGCTATGCGCCTTCAGCATATCCAGCACCTGATCCCACTGGGTAATTTCCACCGGGCGCTGCACCGGACGCGTCATCGGCGGTACTGGCGGCTGGGGGTTTCGGTGTGCG‑GCCAAATAACCCCCACACGGCCGCGGGGGGGTGAGCTTCCTCATCTCCCCCGTCTTATACGCCCCCTGCACGGGGGTGGTAATGACGccccgtcccccgc                                                  >  1:2167/1‑240 (MQ=255)
                                                                                                                                                                                                         ggTGTGCTTTTGCTTAACGG‑AACAATGGAACTGTCGCTATGCGCCTTCAGCATATCCAGCACCTGATCCCACTGGGTAATTTCCACCGGGCGCTGCACCGGACGCGTCATCGGCGGTACTGGCGGCTGGGCTTTTCGTTGTGGGGCCAAAAAAACCC‑AAAGCGGCCGCGGGGTGGTGGGCTTCCTCGTCTCCAGCGGCTGGTACGCCTGCTTCACGGGGCTGGTACTGACGCCGTGCTcccgtctttaggt                                           >  1:9723/1‑240 (MQ=255)
                                                                                                                                                                                                          gtgtGCTTTTGCTTAACGG‑AACAATGGAACTGTCGCTATGCGCCTTCAGCATATCCAGCACCTGATCCCACTGGGTAATTTCCACCGGGCGCTGCACCGGACGCGTCATCGGCGGTACTGGCGGCTGGGCTTTACGTTGTGCG‑ACAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTGCTGATACTGACGCCGTGCTCCTGACTTAAGCTg                                          <  1:204436/251‑1 (MQ=255)
                                                                                                                                                                                                                        aaCGG‑AACAATGGAACTGGCGCTATGCGCCTGCAGCATATCCAGCACCTGATCCCTCTGGGTAATTTCCACCGGGCGCTGCACCGGACGCGTCATCGGCGGTACTGGCGGCTGGGCTTTACGTTGTGCG‑ACAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTGCTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCa                             <  2:237116/250‑1 (MQ=255)
                                                                                                                                                                                                                                                aTGCGCCTTCAGCATATCCAGCACCTGATCCCACTGGGTAATTTCCACCGGGCGCTGCACCGGACGCGTCATCGGCGGTACTGGCGGCTGGGCTTTACGTTGTGCG‑ACAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTGCTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAATTTCTCCCCGTGACGATACAg       <  1:159828/249‑1 (MQ=255)
                                                                                                                                                                                                                                                   cgcCTTCAGCATAACCAGCCACTGAACCCACTGGGTAATTTCCACCGGGCGCTGCTCCGGACGCGTCATCGGCGGTACTGGCGGCTGGGCTTTACGTTGTGCG‑ACAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTGCTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAATTTCTCCCCGTGACGATACAGCCCtt  <  2:166310/251‑1 (MQ=255)
                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                      
TCGAGTTATGGCAGCCGCTGGTGATGATGATGTCATCGGCGGTGACCACCGAGCCGCTGTCGAGCATCAGGCGGGCAATCTGCTCTCGCAATACTCGCTGACCGGCTAACAAGTCATAACCGAGAACGGTTTGCAGATTATGCTGCACCACCCGGCTTAGCTCCCGCCACAGCGG‑TTTCAGGCTGGGCGCTTCGACATCCGGTGTGCTTTTGCTTAACGG‑AACAATGGAACTGTCGCTATGCGCCTCCAGCATATCCAGCACCTGATCCCACTGGGTAATTTCCACCGGGCGCTGCACCGGACGCGTCATCGGCGGTACTGGCGGCTGGGCTTTACGTTGTGCG‑ACAAAATAACCCGAACGCGGCTGCGGAGTGATGAGCTTCATCGTCTCCAGCGTCTGATACGCCTGCTGCACGGTGCTGATACTGACGCCGTGCTCCTGACTTAAGCTGCGCACCGACGGCAATTTCTCCCCGTGACGATACAGCCCTT  >  NC_000913/4571034‑4571525

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑