Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,570,550	C→T	S342N (AGC→AAC)	yjiR ←	fused putative DNA‑binding transcriptional regulator/putative aminotransferase YjiR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,570,550	0	C	T	100.0%	88.9 / NA	30	S342N (AGC→AAC)	yjiR	fused putative DNA‑binding transcriptional regulator/putative aminotransferase YjiR
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (15/15);  total (15/15)

TTCATGCGGCACAGCTGCCGCGCGACGCAGACCATATCGACCTGTTCAGGCAATTCTATCCACAGTAAAAATCCGCCTTTCGGGCGCGTAATACAGATTTCGCAGGGAAAATATTCCCGTATCCAGCAGGTATAAAGCGCCAAATTGCGCTGATAGATCTGCCGCATCCGCCGGATATGGCGATGATAGTGACCTTCCAGCACAAACGTTGCCGCCGCCATTTGCGTGGACGGCACATTAAAGCTGCTGATGGCGTATTTCATATGCATCAGTTTATCGTGATAACGCCCCGGTGCGACCCAACCCACGCGCAGGCCTGGTGCAATACTTTTACTGAACGAGCTGCACAACAGCACTCGCCCGTCGATATCCCAGGAATGAATGGTCCGCGGGCGCGGATACTCCGTCGCCAGTTCGCCATAGACATCATCTTCAAAAATCACAATATCATGACGCTGAGCGAG  >  NC_000913/4570307‑4570770                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                             ttCATGCGGCTCAGCGGCCGCGCGACGCAGACCATATCGACCTGTTCAGGCAACTCTATCCACAGTAAAAATCCGCCTTTCGGGCGCGTAATACAGATTTCGCAGGGAAAATATTCCCGTATCCAGCAGGTATAAAGCGCCAAATTGCGCTGATAGATCTGCCGCATCCGCCGGATATGGCGATGATAGTGACCTTCCAGCACAAACGTTGCCGCCGCCATTTGCGTGGACGGCACATTAAAGTTGCTGa                                                                                                                                                                                                                        <  1:2717/250‑1 (MQ=255)                        gACGCAGACCATATCGACCTGTTCAGGCAATTCTATCCACAGTCAAAATCCGCCTTTCGGGCGCGTCATACAGATTTCGCAGGGAAAATATTCCCGTATCCAGCAGGTATAAAGCGCCAAATTGCGCTGATAGATCTGCCGCATCCGCCGGATATGGCGATGATAGTGACCTTCCAGCACAAACGTTGCCGCCGCCATTTGCGTGGACGGCACATTAAAGTTGCTGATGGCGTATTTCATATGCATCAGtt                                                                                                                                                                                                >  1:270472/1‑251 (MQ=255)                           gCAGACCATATCGACCTTTTCAGGCAATTCTATCCACAGTAAAAACCCGCCTTTCGGGCGCGTAATACAGATTTCGCAGGGAAAATATTCCCGTATCCAGCAGGTATAAAGCGCCAAATTGCGCTGATAGATCTGCCGCATCCGCCGGATATGGCGATGATAGTGACCTTCCAGCACAAACGTTGCCGCCGCCATTTGCGTGGACGGCACATTAAAGTTGCTGATGGCGTATTTCATATGCATCAGTTTAt                                                                                                                                                                                             <  2:172398/251‑1 (MQ=255)                                ccATATCGACCTGTTCAGGCAATTCTATCCACAGTAAAAATCCGCCTTTCGGGCGCGTAATACAGATTTCGCAGGGAAAATATTCCCGTATCCAGCAGGTATAAAGCGCCAAATTGCGCTGATAGATCTGCCGCATCCGCCGGATATGGCGATGATAGTGACCTTCCAGCACAAACGTGGCCGCCGCCATTTGCGTGGACGGCACATTAAAGTTGCTGATGGCGTATTTCATATGCATCAGTTTATCGTGa                                                                                                                                                                                        <  2:158962/251‑1 (MQ=255)                                       gACCTGTTCAGGCAATTCTATCCACAGTAAAAATCCGCCTTTCGGGCGCGTAATACAGATTTCGCAGGGAAAATATTCCCGTCTCCAGCAGGTATAAAGCGCCAAATTGCGCTGATAGATCTGCCGCATCCGCCGGATATGGCGATGATAGTGACCTTCCAGCACAAACGTTGCCGCCGCCATTTGCGTGGACGGCACATTAAAGTTGCTGATGGCGTATTTCATATGCATCAGTTTATCGTGATAACGcc                                                                                                                                                                                 >  1:204500/1‑251 (MQ=255)                                         ccTGTTCAGGCAATTCTATCCACAGTAAAAATCCGCCTTTCGGGCGCGTAATACAGATTTCGCAGGGAAAATATTCCCGTATCCAGCAGGTATAAAGCGCCAAATTGCGCTGATAGATCTGCCGCATCCGCCGGATATGGCGATGATAGTGACCTTCCAGCACAAACGTTGCCGCCGCCATTTGCGTGGACGGCACATTAAAGTTGCTGATGGCGTATTTCATATGCATCAGTTTATCGTGATAACGcccc                                                                                                                                                                               <  1:202508/251‑1 (MQ=255)                                                    aaTTCTATCCACAGTAAAAATCCGCCTTTCGGGCGCGTAATACAGATTTCGCAGGGAAAATATTCCCGTATCCAGCAGGCATAAAGCCCCACATTGCGCTGATAGATCTGCCGCATCCGACGGCTATGGCGATGATAGTGACCTTCCAGCACAAACGTTGCCGCCGCCATTTGCGCGGCCGGCACATTAAAGTTGCTGATGGCGAATTTCATATGCATCAGTTTATCGTGATAACGCCCCGGTGCGaccc                                                                                                                                                                     >  1:195774/1‑250 (MQ=255)                                                                   aaaaaGCCGCCTTTCGGGCGCGTAATAAAGATTTCGCAGGGAAAATATTCCCGTATCCAGCAGGTATAAAGCGCCAAATTGCGCTGATAGATCTGCCGCATCCGCCGGATATGGCGATGATAGTGACCTTCCAGCACAAACGTTGCCGCCGCCATTTGCGTGGACGGCACATTAAAGTTGCTGATGGCGTATTTCATAGGCATCAGTTTATCGTGATAACGCCCCGGTGCGACCCAACCCACGCGCAGGcc                                                                                                                                                     <  2:270472/251‑1 (MQ=255)                                                                                   ggCGCGTAATACAGATTTCGCAGGGAAAATATTCACGTATCCAGCAGGTATAAAGCGCCAAATTGCGCTGATAGATCTGCCGCATCCGCCGGATATGGCGATGATAGTGACCTTCCAGCACAAACGTTGCCGCCGCCATTTGCGTGGACGGCACATTAAAGTTGCTGATGGCGTATTTCATATGCATCAGTTTATCGTGATAACGCCCCGGTGCGACCCAACCCACGCGCAGGCCTGGTGCAATACtttt                                                                                                                                      >  2:59882/1‑250 (MQ=255)                                                                                               aGATTTCGCAGGGAAAATATTCCCGTATCCAGCAGGTATAAAGCGCCAAATTGCGCTGATAGATCTGCCGCATCCGCCGGATATGGCGATGATAGTGACCTTCCAGCACAAACGTTGCCGCCGCCATTTGCGTGGACGGCACATTAAAGTTGCTGATGGCGTATTTCATATGCATCAGTTTATCGTGATAACGCCCCGGTGCGACCCAACCCACGCGCAGGCCTGGTGCAATACTTTTACTGAACGAGCTg                                                                                                                         >  1:27273/1‑251 (MQ=255)                                                                                                     cGCAGGGAAAATATTCCCGTATCCAGCAGGTATAAAGCGCCAAATTGCGCTGATAGATCTGCCGCATCCGCCGGATATGGCGATGATAGTGACCTTCCAGCACAAACGTTGCCGCCGCCATTTGCGTGGACGGCACATTAAAGTTGCTGATGGCGTATTTCATATGCATCAGTTTATCGTGATAACGCCCCGGTGCGACCCAACCCACGCGCAGGCCTGGTGCAATACTTTTACTGAACGAGCTGCacaac                                                                                                                   <  1:185461/251‑1 (MQ=255)                                                                                                       cAGGGAAAATATTCCCGTATCCAGCAGGTATAAAGCGCCAAATTGCGCTGATAGATCTGCCGCATCCGCCGGATATGGCGATGATAGTGACCTTCCAGCACAAACGTTGCCGCCGCCATTTGCGTGGACGGCACATTAAAGTTGCTGATGGCGTATTTCATATGCATCAGATTATCGTGATAACGCCCCGGGGCGACCCAACCCACGCGCAGGCCTGGTGCCATACTTTTACTGAACGAGATGCacaac                                                                                                                   >  1:172602/1‑249 (MQ=255)                                                                                                        aGGGAAAATATTCCCGTATCCAGCAGGTATAAAGCGCCAAATTGCGCTGATAGATCTGCCGCATCCGCCGGATATGGCGATGATAGTGACCTTCCAGCACAAACGTTGCCGCCGCCATTTGCGTGGACGGCACATTAAAGTTGCTGATGGCGTATTTCATATGCATCAGTTTATCGTGATAACGCCCCGGTGCGACCCAACCCACGCGCAGGCCTGGTGCAATACTTTTACTGAACGAGCTGCCCAACAGc                                                                                                                >  2:219979/1‑251 (MQ=255)                                                                                                            aaaaTATTCCCGTTTCCAGCAGTTATAAATCGCCAAATTGCGCTGTGAGCTCTGACGCATCCGCCGTATATGGCGATGATAGTGCCCTTCCAGCACAAACGTTGCCGCCGCCATTTGCGTGGACGGCACATTAAAGTTGCTGATGGCGTATTTCATATGCATCAGTTGATCGTGATAACGCCCCGGTGCGACCCAACCCACGCGCAGGCCTGGTGCAATACTCTTACTGCACGAGCTGCTCAACAGCACTc                                                                                                            <  2:236459/251‑1 (MQ=255)                                                                                                               atatTCCCGTATACAGAAGGCATAAAGCGCCAAATTCCGCTGATAGATCTGCCGCATCCCCCGGATATGGCGATGATAGTCACCTTCCAGCACAAACGTTCCCGCCGCCATTAGCGTGGACGGCACATTAAAGTTGCTGCTGGCGTATTTCCTATGCATCAGTTTATCGTGATAACACCCCGGTGCGACCCAACCCACGCGCCGGCCTGTTGCAATACTTTTACTGAACGAGCTGCACAACCGCACtgccc                                                                                                         >  2:112414/1‑247 (MQ=255)                                                                                                                tatTCCCGTATCCAGCAGGTATAAAGCGCCAAATTGCGCTGATAGATCTGCCGCATCCGCCGGATATGGCGATGATAGTGACCTTCCAGCACAAACGTTGCCGCCGCCATTTGCGTGGACGGCACATTAAAGTTGCTGATGGCGTATTTCATATGCATCAGTTTATCGTGATAACGCCCCGGTGCGACCCAACCCACGCGCAGGCCTGGTGCAATACTTTTACTGAACGAGCTGCACAACAGCACTCGccc                                                                                                        >  1:307306/1‑251 (MQ=255)                                                                                                                                      aaaGCGCCAAATTGCGCTGATAGATCTGCCGCATCCGCCGGATATGGCGATGATAGTGACCTTCCAGCACAAACGTTGCCGCCGCCATTTGCGTGGACGGCACATTAAAGTTGCTGATGGCGTATTTCATATGCATCAGTTTATCGTGATAACGCCCCGGTGCGACCCAACCCACGCGCAGGCCTGGTGCAATACTTTTACTGAACGAGCTGCACAACAGCACTCGCCCGTCGATATCCCAGGaatgaatg                                                                                  >  1:169816/1‑251 (MQ=255)                                                                                                                                                           agagcTGCCGCATCCGCCGGATATGGCGATGATAGTGACCTTCCAGCACAAACGGTGCCGCCGCCATTTGCGTGGACGGCACATTAAAGTTGCTGATGGCGTATTTCATATGCATCAGTTTATCGTGATAACGCCCCGGTGCGACCCAACCCACGCGCAGGCCTGGTGCAATACTTTTACTGAACGAGCTGCACAACAGCACTCGCCCGTCGATATCCCAGGAATGAATGGTCCGCGGGCGCGGATACTcc                                                             <  2:71259/247‑1 (MQ=255)                                                                                                                                                           aGATCTGCCGCATCCGCCGGATATGGCGATGATAGTGACCTTCCAGCACAAACGTTGCCGCCGCCATTTGCGTGGACGGCACATTAAAGTTGCTGATGGCGTATTTCATATGCATCAGTTTATCGTGATAACGCCCCGGTGCGACCCAACCCACGCGCAGGCCTGGTGCAATACTTTTACTGAACGAGCTGCACAACAGCACTCGCCCGTCGATATCCCAGGAATGAATGGTCCGCGGGCGCGGATACTcc                                                             <  1:10937/251‑1 (MQ=255)                                                                                                                                                                      cactatcatcgccggCGATGATAGTGACCTTCCAGCACAAACGTTGCCGCCGCCATTTGCGTGGACGGCACATTAAAGTTGCTGATGGCGTATTTCATATGCATCAGTTTATCGTGATAACGCCCCGGTGCGACCCAACCCACGCGCAGGCCTGGTGCAATACTTTTACTGAACGAGCTGCACAACAGCACt                                                                                                             <  1:71102/179‑1 (MQ=255)                                                                                                                                                                      cactatcatcgccggCGATGATAGTGACCTTCCAGCACAAACGTTGCCGCCGCCATTTGCGTGGACGGCACATTAAAGTTGCTGATGGCGTATTTCATATGCATCAGTTTATCGTGATAACGCCCCGGTGCGACCCAACCCACGCGCAGGCCTGGTGCAATACTTTTACTGAACGAGCTGCACAACAGCACt                                                                                                             >  2:71102/14‑192 (MQ=255)                                                                                                                                                                                   ggCGATGATAGTGACCTTCCAGCACAAACGTTGCCGCCGCCATTTGCGTGGACGGCACATTAAAGTTGCTGATGGCGTATTTCATATGCATCAGTTTATCGTGATAACGCCCCGGTGCGACCCAACCCACGCGCAGGCCTGGTGCAATACTTTTACTGAACGAGCTGCACAACAGCACTCGCCCGTCGATATCCCAGGAATGAATGGTCCGCGGGCGCGGATACTCCGTCGCCAGTTCGCCATAGAcatc                                      <  2:307306/250‑1 (MQ=255)                                                                                                                                                                                             gTGACCTTCCAGCACAAACGTTGCCGCCGCCATTTGCGTGGACGGCACATTAAAGTTGCTGATGGCGTATTTCATATGCATCAGTTTATCGTGATAACGCCCCGGTGCGACCCAACCCACGCGCAGGCCTGGTGCAATACTTTTACTGAACGAGCTGCACAACAGCACTCGCCCGTCGATATCCCAGGAATGAATGGTCCGCGGGCGCGGATACTCCGTCGCCAGTTCGCCATAGACATCATCTTCaaaaa                           <  1:80166/251‑1 (MQ=255)                                                                                                                                                                                              tGACCTTCCAGCACAAACGTTGCCGCCGCCATTTGCGTGGACGGCACATTAAAGTTGCTGATGGCGTATTTCATATGCATCAGTTTATCGTGATAACGCCCCGGTGCGACCCAACCCACGCGCAGGCCTGGTGCAATACTTTTACTGAACGAGCTGCACAACAGCACTCGCCCGTCGATATCCCAGGTATGAATGGTCCGCGGGCGCGGATACTCCGTCGCCAGTTCGCCATAGACATCATCTTCAAAAAt                          >  1:192413/1‑251 (MQ=255)                                                                                                                                                                                                  cTTCCAGCAAAAACGTTGCAGCCGCCATTTGCGTGGACGGCACATTAAAGTTGATGATGGCGTCTTTCATATGCATCAGTTTATCGTGATAAAGCCCCGGTGCGACCTCACCCACGCCCAGGCCTGGTGCAATACTTTTACTGAACGAGCTGCACAACAGCACTCGCCCGTCGATATCCCAGGAATGAATGGTCCGCGCGCGCGGATACTCCGTCGCCAGTTCGCCATAGACATCATCTTCAAACATcac                       >  2:45441/1‑250 (MQ=255)                                                                                                                                                                                                     ccAGCGCAAACGTGGCCGCCGCCATTTGCGTGGACGTCACATTAAGGTTGCTGATGGCGTATTTCATATGCAGCAGTTTATCGTGAAAACGCCCCGGTGCGACCCACCCCACGAGCAGGCCAAGTGCAATACTTTTACTGAACGATCTGCACAACAGCACGCGCCCGTCGATATCCCAGGAATGAATGGGCCCCGGGCACGGATACACAGGCGCCAGGTCGCCATAGACATCATCTTCAAAAATCACAata                   <  2:143647/251‑1 (MQ=255)                                                                                                                                                                                                        gCACAAACGTTGCCGCCGCCATTTGCGTGGACGGCACATTAAAGTTGCTGATGGCGTATTTCATATGCATCAGTTTATCGTGATAACGCCCCGGTGCGACCCAACCCACGCGCAGGCCTGGTGCAATACTTTTACTGAACGAGCTGCACAACAGCACTCGCCCGTCGATATCCCAGGAATGAATGGTCCGCGGGCGCGGATACTCCGTCGCCAGTTCGCCATAGACATCATCTTCAAAAATCACAATATc                 >  1:220028/1‑250 (MQ=255)                                                                                                                                                                                                             aaCGTTGCGGCCGTCATTTGCGTGGACGTCACATTAAAGTGGCTGATGGCGTATTTCATATGCAACAGTTTATCGTGATAACGCCCCGGTGCGACCCAACCCTCGCGCAGGCCTGGTGCAATACTTTTACTGAACGAGCTGCACAACAGCACTCGGCCGTCGATCTCCCAGGAATGAATGGGCCGCGGGCGCGGATAATCCGTCGCCAGGTCGCCATAGTCATCATCTTCAAAAATCACAATATCATgccg           <  1:98633/251‑4 (MQ=255)                                                                                                                                                                                                             aaCGTTGCCGCCGCCATTTGCGTGGACGGCACATTAAAGTTGCTGATGGCGTATTTCATATGCATCAGTTTATCGTGATAACGCCCCGGTGCGACCCAACCCACGCGCAGGCCTGGTGCAATACTTTTACTGAACGAGCTGCACAACAGCACTCGCCCGTCGATATCCCAGGAATGAATGGTCCGCGGGCGCGGATACTCCGTCGCCAGTTCGCCATAGACATCATCTTCAAAAATCACAATAGCATGACg           <  1:230229/251‑1 (MQ=255)                                                                                                                                                                                                                        cgccATTTGCGTGGACGGCACATTAAAGTTGCTGATGGCGTATTTCATATGCATCAGTTTATCGTGATAACGCCCCGGTGCGACCCAACCCACGCGCAGGCCTGGTGCAATACTTTTACTGAACGAGCGGCACAACAGCACTCGCCCGTCGATATCCCAGGAATGAATGGTCCGCGGGCGCGGATACTCCGTCGCCAGTTCGCCATAGACATCATCTTCAAAAATCACAATATCATGACGCTGAGCgag  >  2:240465/1‑249 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                             TTCATGCGGCACAGCTGCCGCGCGACGCAGACCATATCGACCTGTTCAGGCAATTCTATCCACAGTAAAAATCCGCCTTTCGGGCGCGTAATACAGATTTCGCAGGGAAAATATTCCCGTATCCAGCAGGTATAAAGCGCCAAATTGCGCTGATAGATCTGCCGCATCCGCCGGATATGGCGATGATAGTGACCTTCCAGCACAAACGTTGCCGCCGCCATTTGCGTGGACGGCACATTAAAGCTGCTGATGGCGTATTTCATATGCATCAGTTTATCGTGATAACGCCCCGGTGCGACCCAACCCACGCGCAGGCCTGGTGCAATACTTTTACTGAACGAGCTGCACAACAGCACTCGCCCGTCGATATCCCAGGAATGAATGGTCCGCGGGCGCGGATACTCCGTCGCCAGTTCGCCATAGACATCATCTTCAAAAATCACAATATCATGACGCTGAGCGAG  >  NC_000913/4570307‑4570770

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑