Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,871,153	T→G	N51T (AAC→ACC)	truD ←	tRNA pseudouridine(13) synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,871,153	0	T	G	100.0%	131.7 / NA	35	N51T (AAC→ACC)	truD	tRNA pseudouridine(13) synthase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (20/15);  total (20/15)

AAAGGCGTTACCTTTCAGCGCGCCTAAACGCAGCTTGCGCTTGTGCCGCGCATACTCCAGCACCTGGCAGCCTTCCAGTTGAAAGGCGCTCAGATCGGGCATTTCCTTGCCCGGCACGCGAGCGCATAACCACTGTTCC‑GTAACAGCATGTTTGTCTTTTTGCCCAGCGAAGC‑TGACTTCACGGGCATGAA‑TTTTCAGGAATTTCGCCAGTGCATCCGCCACAAAACGGGTATTGCAGCCGTTTTTGAGGATTCTAACCAGAATATGCTCACCTTCACCATCAGGCTCAAAGCCCAAATCTTCCACCACCACAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCGGTTTACCGTGGAGGTAAGTGAGATTATCAAACTCAATCATTTTGTTGCCTTAATGAGTAGCGCCACCGCTTCACAGGCAATCCCTTCCCCACGTCCGGTAAATCCCAGTTTTTCCGTAGTAGTGGCTTTC  >  NC_000913/2870912‑2871394                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                  aaaGGCGTTACCTTTCAGCGCGCCTAAACGCAGCTTGCGCTTGTGCCGCGCATACTCCAGCACCTGGCAGCCTTCCAGTTGAAAGGCGCTCAGATCGGGCATTTCCTTGCCCGGCACGCGAGCGCATAACCACTGTTCC‑GTAACAGCATGTTTGTCTTTTTGCCCAGCGAAGC‑TGACTTCACGGGCATGAA‑TTTTCAGGAATTTCGCCAGTGCATCCGCCACAAAACGGGTATTGCAGCCGGTTTTGAgg                                                                                                                                                                                                                                           >  1:209610/1‑250 (MQ=255)    ggCGTTACCTTTCAGCGCGCCTAAACGCAGCTTGCGCTTGTGCCGCGCATACTCCAGCACCTGGCAGCCTTCCAGTTGAAAGGCGCTCAGATCGGGCATTTCCTTGCCCGGCACGCGAGCGCATAACCACTGTTCC‑GTAACAGCATGTTTGTCTTTTTGCCCAACGAAGC‑TGACTTCACGGGCATGAA‑TTTTCAGGAATTTCGCCAGTGCATCCGCCACAAAACGGGTATTGCAGCCGGTTTTGAGGATTc                                                                                                                                                                                                                                       >  2:89414/1‑251 (MQ=255)                cAGCGCGCCTAAACGCAGCTTGCGCTTGTGCCGCGCATACTCCAGACCCTGGCAGCCTTCCAGTTGACATCCGCTCAGATCGCGCCTTTCCTTGCCCGGCACGCGAGCGCATAACCACTGTTCC‑GTACCAGCATGTTTGTCTTTTTGCCCAGCGAAGC‑TGACTTCACGAGCATGAC‑TTTTCAGGAATTTCGCCCGTGCATCCGCCACAAACCGGGGATTGCAGCCGGTTTTGAGGATTCTAACCAGAatat                                                                                                                                                                                                                           >  1:121976/1‑251 (MQ=255)                             cGCAGCTTGCGCTTGTGCCGCGCATACTCCAGCACCTGGCAGCCTTCCAGTTGAAAGGCGCTCAGATCGGGCATTTCCTTGCCCGGCACGCGAGCGCATAACCACTGTTCC‑GTAACAGCATGTTTGTCTTTTTGCCCAGCGACGC‑TGACTTCACGGGCATGAA‑TTTTCAGGAATTTCGCCAGTGCATCCGCCACAAAACGGGTATTGCAGCCGGTTTTGAGGATTCTAACCCGAATATGCTCACCTTCAcc                                                                                                                                                                                                              >  2:57590/1‑251 (MQ=255)                                aGCTTGCGCTTGTGCCGCGCATACTCCAGCACTTGGCAGCCTCCCAGTTAAAAGGCGCTCATATCGGGCATTTCCGTGCCCGGCACGCGAGCTCATAACCCCTGTTCC‑GTAACAGCATGTTGGTCTTTTGGCCCAGCGAAGC‑TGACTTCGCGGGCATGAA‑TTTTCAGGAATTTGGCCAGTGCATCCGCCACAAAACGGGTATTGCAGCCGGTTTTGAGGATTCTAACCAGAATATGCTCACCTTCACCATc                                                                                                                                                                                                           <  1:47291/251‑1 (MQ=255)                                                      aCTCCAGCACCTGGCAGCCTTCCAGTTGAAAGGCGCTCAGCTCGGGCATTTCCTTGCCCGGCACGCGAGCGCATAACCACTGTTCC‑GTAACAGCATGTTTGTCTTTTTGCCCAGCGAAGC‑TGACTTCACGGGCATGAA‑TTTTCAGGAATTTCGCCAGTGCATCCGCCACAAAACGGGTATTGCAGCCGGTTTTGAGGATTCTAACCAGAATATGCTCACCTTCACCATCAGGCTCAAAGCCCAAATCTTcc                                                                                                                                                                                     >  2:142887/1‑251 (MQ=255)                                                              aCCTGGCCGCCGTCCAGTAGAAAGGCCCTAAGATCGGGCATTTCCTTGCCCGGCACGCGAGCGCATACCCACTGTTCC‑GTAACAGCATGTTTGTCTTTTTGCCCAGCGAAGC‑TGACTTCACGGGCATGAA‑TTTTCAGGAATTTCGCCAGTGCATCCGCCACAAAACGGGTATTGCAGCCGGTTTTGTGGATTCTAACCAGAATATGCTCACCTTCACCATCAGGCTCAACGCCCAAATCTTCaaccaccac                                                                                                                                                                             >  2:42574/1‑251 (MQ=255)                                                               ccTGGCAGCCTTCCAGTTGAAAGGCGCTCAGATAGGGCATTTCCTTGCCAGGCACGCGAGCGCATAACAACTGTTCC‑GTAACAGCCAGTTTGTCTTTTTGCCCAGCGAAGC‑TGACTTCACGGGCATGAA‑TTTTCAGGAATTTCGCCAGTGCATCCGCCACAAAACGGGTATTGCAGCCGGTTTTGAGGATTCTAACCAGCATATGCTCACCTTCACCATCAGGCTCAAAGCCCAAATCTTCcaccaccac                                                                                                                                                                             >  1:283058/1‑250 (MQ=255)                                                                   gcatcctgccAGTTGAAAGGCGCTCAGTGCGGGATTTTTCTTGCCCGGCACGCTAGCGAATAACCTCTGTCCC‑GTAACAGCATGTTTGTCTTTTTCCCCAGCGAAGC‑TGACTTCAGGGGCATAAA‑TTTTCAGGAATTTCGCCGTTGCATCCGCCACAAAACGGGTATTGCAGCCGGTTTTGAGGATTCTAACCAGAATATGCTCACCTTCACCATCAGGCTCAAAGCCCAAATCTTCCACCACCACAAAGt                                                                                                                                                                        <  1:269008/243‑1 (MQ=255)                                                                          tccatttaaaaGGCCCTCAGATCGGGCTTTTCCTTGCCCGGCCCGCGAGCGCATACCCTCTTTTCC‑TAAACCTCATTTTTGTCTTTTTCCCCAGCGAAGC‑TGCCTTCCCGGGCATGAA‑TTTTCAGAAATTTCGCCCTTGCATCCGCCACAAAACGGGTATTGCAGCCGGTTTTGAGGATTCTAACCAGAATATGCTCACCTTCACCAACAGGCTCAAAGCCCAAATCTTCCACCACCACAAAGTCTTCCg                                                                                                                                                                  <  1:236331/242‑1 (MQ=255)                                                                                        ccTCAGACCGGGCATTTCCTTGCCCGGCACGCGAGCGCATAACCATTGTTCC‑GTAACAGCATGTTTGTCTTTTTGCCCAGCGAAGC‑TGACTTCACGGGCTTGAA‑TTTGCAGGAATTTCGCCAGTGCATCCGCCACAAAACGGGTATTGCAGCCGGTTTTGAGGATTCTAACCAGAATATGCTCACCTTCACCATCAGGCTCAAAGCCCAAATCTTCCACCACCACAAAGTCTTCCGGATTGGCTTTcagca                                                                                                                                                   <  2:219942/250‑1 (MQ=255)                                                                                            aGATCGGGCATTTCCTTGCCCGGCACGCGAGCGCATAACCACTGTTCC‑GTAACAGCATGTTTGTCTTTTTGCCCAGCGAAGC‑TGACTTCACGGGCATGAA‑TTTTCAGGAATTTCGCCAGTGCATCCGCCACAAAACGGGTATTGCAGCCGGTTTTGAGGATTCTAACCAGAATATGCTCACCTTCACCATCAGGCTCAAAGCCCAAATCTTCCACCACCACAAAGTCTTCCGGATTGGCTTTCAGCAGccc                                                                                                                                               >  1:182676/1‑251 (MQ=255)                                                                                                      tttCCTTGCCCGGCACGCGAGCGCATAACCACTGTTCC‑GTAACAGCATGTTTGTCTTTTTGCCCAGCGAAGC‑TGACTTCACGGGCATGAA‑TTTTCAGGAATTTCGCCAGTGCATCCGCCACAAAACGGGTATTGCAGCCGGTTTTGAGGATTCTAACCAGAATATGCTCACCTTCACCATCAGGCTCAAAGCCCAAATCTTCCACCACCACAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGc                                                                                                                                     >  1:47619/1‑251 (MQ=255)                                                                                                          cTTGCCCGGCACGCGAGCGCATAACCACTGTTCC‑GTAACAGCATGTTTGTCTTTTTGCCCAGCGAAGC‑TGACTTCACGGGCATGAA‑TTTTCAGGAATTTCGCCAGTGCATCCGCCACAAAACGGGTATTGCAGCCGGTTTTGAGGATTCTAACCAGAATATGCTCACCTTCACCATCAGGCTCAAAGCCCAAATCTTCCACCACCACAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCGGtt                                                                                                                                 >  2:231684/1‑251 (MQ=255)                                                                                                              cccGGCACGCGAGCGAATAACCACGGTTCC‑GTAACAGCATGTTTGTCTTTTTGCCCAACGAAGC‑TGACTTCACGGGCATGAA‑TTTTCAGGAATTTCGCCAGTGCATCCGCCACAAAACGGGTATTGCAGCCGGTTTTGAGGATTCTAACCAGAATATGCTCACCTTCACCATCAGGCTCAAAGCCCAAATCTTCCACCACCACAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCGGTTTAcc                                                                                                                             <  1:89414/251‑1 (MQ=255)                                                                                                                        gAGCGCATAACCAC‑GTTCCCGTAACAGCATGTTTGTCTTTGTGCCCAGCGACGC‑TGCCTTCACGGGCATGAA‑TTTTCAGGAATTCCGCCAGTGCATCCGCCACAAAACGGGTATTGCAGCCGGTTTTGAGGATTCTAACCAGAATATGCTCACCTTCACCATCAGGCTCAAAGCCCAAATCTGCCACCACCACAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCGGTTTACCGTGGAGGTaa                                                                                                                   <  1:142887/251‑1 (MQ=255)                                                                                                                               tAACCACTGTTCC‑GTAACAGCATGTTTGTCTTTTTGCCCAGCGAAGC‑TGACTTCACGGGCATGAA‑TTTTCAGGAATTTCGCCAGTGCATCCGCCACAAAACGGGTATTGCAGCCGGTTTTGAGGATTCTAACCAGAATATGCTCACCTTCACCATCAGGCTCAAAGCCCAAATCTTCCACCACCACAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCGGTTTACCGTGGAGGTAAGTgagct                                                                                                            >  1:176223/1‑249 (MQ=255)                                                                                                                                     acttttcctaaaCAGCAGGTTTGTCTATGTCCCCAGC‑AAGCCTGACTCCGCGGGCTTGAA‑TTTCCAGAAATTTCGTCAGTCAATCCGCCACAAAACGGGTATTGCAGCCGGTTTTGAGGATTCTAACCAGAATATGCTCACCTTCACCATCAGCCTCAAAGCCCAAATCTTCCTCCACCACAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCGGTTTACCGTGGAGGTAAGTGAGATTATCa                                                                                                       <  1:118440/241‑1 (MQ=255)                                                                                                                                        ttCC‑GTAACAGCATGTTTGTCTTTTTGCCCAGCGAAGC‑TGACTTCACGGGCATGAA‑TTTTCAGGAATTTCGCCAGTGCATCCGCCACAAAACGGGTATTGCAGCCGGTTTTGAGGCTTCTAACCAGAATATGCTCACCTTCACCATCAGGCTCAAAGCCCAAATCTTCCACCACCACAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCGGTTTACCGTGGAGGTAAGTGCGCTTATCAAACt                                                                                                   >  2:94698/1‑251 (MQ=255)                                                                                                                                                 cAGCATGTTTGTCTTTTTGCCCAGCGAAGC‑TGACTTCACGGGAATGAA‑TTTTCAGGAATTTCGCCAGTGCATCCGCCACAAAACGGGTATTGCAGCCGGTTTTGAGGATTCTAACCAGAATATGCTCACCTTCACCATCAGGCTCAAAGCCCAAATCTTCCACCACCACAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCGGTTTACCGTGGAGGTAAGTGAGATTATCAAACTCAATCAtt                                                                                           <  1:164430/251‑1 (MQ=255)                                                                                                                                                         ttGTTTTTTTGCCCAGCGAGGC‑GGACTTCGGGGGTATGAA‑TTTTCAGGATTTTCGCCAGTGCATCCGCCACAAAACGGGTATTGCAGCCGGTTTTGAGGATTCTAACCAGAATATGCTCACCTTCACCATCAGGCTCAAAGCCCAAATCTTCCACCACCACAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCGGTTTACCGTGGAGGTAAGTGAGATTATCAAACTCAATCATTTTGTTGc                                                                                    <  2:87338/250‑1 (MQ=255)                                                                                                                                                          tGTCTTTTTGCCCAGGGACGC‑TGACTTCTCGGGTAGTAA‑TTTTCAGGAATTCCGCGAGGACATCCGCCACAAAACGGGTATTGCAGCCGGTTTTGAGGATTCTAACCAGAATATGCTCACCTTCACCATCAGGCTCAAAGCCCAAATCTTCCACCACCACAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCGGTTTACCGTGGAGGTAAGTGAGATTATCAAACGCAATCATTTTGTTGcc                                                                                   <  1:209701/250‑1 (MQ=255)                                                                                                                                                           ttgtttttCCCCAGGGAAGC‑TGTCTTGACGGG‑TTGAAATTTACAGAATTTTCCCCATTGCACCCGCCACAAAACGGGTATTGCAGCCGGTTTTGAGGATTCTAACCAGAATATGCTCACCTTCACCATCAGGCTCAAAGCCCAAATCGTCCACCACCACAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCGGTTTACCGTGGAGGTAAGTGAGATTATCAAACTCAATCATTTTGTTGCCt                                                                                  <  2:281943/247‑1 (MQ=255)                                                                                                                                                                                  aCTTCACGGGCATGAA‑TTTTCAGGAATTTCGCCAGTGCATCCGCCACAAAACGGGTATTGCAGCCGGTTTTGAGGATTCTAACCAGAATATGCTCACCTTCACCATCAGGCTCAAAGCCCAAATCTTCCACCACCACAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCGGTTTACCGTGGAGGTAAGTGAGATTATCAAACTCAATCATTTTGTTGCCTTAATGAGTAGCGCCACCGCTTcc                                                           >  1:148918/1‑250 (MQ=255)                                                                                                                                                                                                      tCAGGAATTTCGCCAGTGCATCCGCCACAAAACGGGTATTGCAGCCGGTTTTGAGGATTCTAACCAGAATATGCTCACCTTCACCATCAGGCTCAAAGCCCAAATCTTCCACCACCACAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCGGTTTACCGTGGAGGTAAGTGAGATTATCAAACTCAATCATTTTGTTGCCTTAATGAGTAGCGCCACCGCTTCACAGGCAATCCCTTCCCCAc                                        >  1:19921/1‑251 (MQ=255)                                                                                                                                                                                                            aTTTCGCCAGTGCATCCGCCACAAAACGGGTATTGCAGCCGGTTTTGAGGATTCTAACCAGAATATGCTCACCTTCACCATCAGGCTCAAAGCCCAAATCTTCCACCACCACAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCGGTTTACCGTGGAGGTAAGTGAGATTATCAAACTCAATCATTTTGTTGCCTTAATGTGTAGCGCCACCGCTTCACAGGCAATCCCTTCCCCACGTCCgg                                  >  1:258838/1‑251 (MQ=255)                                                                                                                                                                                                                cGCCAGTGCATCCGCCACAAAACGGGTATTGCAGCCGGTTTTGAGGATTCTAACCAGAATATGCTCACCTTCACCATCAGGCTCAAAGCCCAAATCTTCCACCACCACAAAGTCTTCCGGCTTGGCTTTCAGCAGCCCGGTGCCTTGCGGTTTACCGTGGAGGTAAGTGAGATTATCAAACTCAATCATTTTGTTGCCTTAATGAGTAGCGCCACCGCTTCACAGGCAATCCCTTCCCCACGTCCGGTaa                               >  1:261646/1‑250 (MQ=255)                                                                                                                                                                                                                   cAGTGCATCCGCCACAAAACGGGTATTGCAGCCGGTTTTGAGGATTCTAACCAGAATATGCTCACCTTCACCATCAGGCTCAAAGCCCAAATCTTCCACCACCACAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCGGTTTACCGTGGAGGTAAGTGAGATTATCAAACTCAATCATTTTGTTGCCTTAATGAGTAGCGCCACCGCTTCACAGGCAATCCCTTCCCCACGTCCGGTAAATcc                           >  2:92372/1‑251 (MQ=255)                                                                                                                                                                                                                    aGTGCATCCGCCACAAAACGTGTATTGCAGCCGGTTTTGAGGATTCTAACCAGAATATGCTCACCTTCACCATCAGGCTCAAAGCCCAAATCTTCCACCACCACAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCGGTTTACCGTGGAGGTAAGTGAGATTATCAAACTCAATCATTTTGTTGCCTTAATGAGTAGCGCCACCGCTTCACAGGCAATCCCTTCCCCACGTCCGGTAAATccc                          >  2:97528/1‑251 (MQ=255)                                                                                                                                                                                                                      cccATCCGCCACAAAACGGGTATTGCAGCCGGTTTTGAGGATTCTAACCAGAATATGCTCACCTTCACCATCAGGCTCAAAGCCCAAATCTTCCACCACCACAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCGGTTTACCGTGGAGGTAAGTGAGATTATCAAACTCAATCATTTTGTTGCCTTAATGAGTAGCGCCACCGCTTCACAGGCAATCCCTTCCCCACGTCCGGTAAATCCCAg                        <  2:47619/249‑1 (MQ=255)                                                                                                                                                                                                                                    aaCGGGTATTGCAGCCGGTTTTGAGGATTCTAACCAGAATATGCTCACCTTCACCATCAGGCTCAAAGCCCAAATCTTCCACCACCACAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCGGTTTACCGTGGAGGTAAGTGAGATTATCAAACTCAATCATTTTGTTGCCTTAATGAGTAGCGCCACCGCTTCACAGGCAATCCCTTCCCCACGTCCGGTAAATCCCAGttttt                   >  2:165737/1‑242 (MQ=255)                                                                                                                                                                                                                                    aaCGGGTATTGCAGCCGGTTTTGAGGATTCTAACCAGAATATGCTCACCTTCACCATCAGGCTCAAAGCCCAAATCTTCCACCACCACAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCGGTTTACCGTGGAGGTAAGTGAGATTATCAAACTCAATCATTTTGTTGCCTTAATGAGTAGCGCCACCGCTTCACAGGCAATCCCTTCCCCACGTCCGGTAAATCCCAGttttt                   <  1:165737/242‑1 (MQ=255)                                                                                                                                                                                                                                         gTATTGCAGCCGGTTTTGAGGATTCTAACCAGAATATGCTCACCTTCACCATCAGGCTCAAAGCCCAAATCTTCCACCACCACAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCGGTTTACCGTGGAGGTAAGTGAGATTATCAAACTCAATCATTTTGTTGCCTTAATGAGTAGCGCCACCGCTTCACAGGCAATCCCTTCCCCACGTCCGGTAAATCCCAGTTTTTCCGTAGTAGTGGCt     <  1:294604/251‑1 (MQ=255)                                                                                                                                                                                                                                             tGCAGCCGGTTTTGAGGATTCTAACCAGAATATGCTCACCTTCACCATCAGGCTCAAAGCCCAAATCTTCCACCACCACAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCGGTTTACCGTGGAGGTAAGTGAGATTATCAAACTCAATCATTTTGTTGCCTTAATGAGTAGCGCCACCGCTTCACAGGCAATCCCTTCCCCACGTCCGGTAAATCCCAGTTTTTCCGTAGTAGTGGCTTTc  >  1:20795/1‑250 (MQ=255)                                                                                                                                                                                                                                             tGCAGCCGGTTTTGAGGATTCTAACCAGAATATGCTCACCTTCACCATCAGGCTCAAAGCCCAAATCGTCCTCCACCACAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCGGTTTACCGTGGAGGTAAGTGAGATTATCAAACTCAAGCATTTTGTTGCCTTAATGAGTAGCGCCACCGCTTCACAGGCAATCCCTTCCCCACGTCCGGTAAATCCCAGTTTTTCCGTAGAAGTGGCTTTc  <  2:123396/250‑1 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                  AAAGGCGTTACCTTTCAGCGCGCCTAAACGCAGCTTGCGCTTGTGCCGCGCATACTCCAGCACCTGGCAGCCTTCCAGTTGAAAGGCGCTCAGATCGGGCATTTCCTTGCCCGGCACGCGAGCGCATAACCACTGTTCC‑GTAACAGCATGTTTGTCTTTTTGCCCAGCGAAGC‑TGACTTCACGGGCATGAA‑TTTTCAGGAATTTCGCCAGTGCATCCGCCACAAAACGGGTATTGCAGCCGTTTTTGAGGATTCTAACCAGAATATGCTCACCTTCACCATCAGGCTCAAAGCCCAAATCTTCCACCACCACAAAGTCTTCCGGATTGGCTTTCAGCAGCCCGGTGCCTTGCGGTTTACCGTGGAGGTAAGTGAGATTATCAAACTCAATCATTTTGTTGCCTTAATGAGTAGCGCCACCGCTTCACAGGCAATCCCTTCCCCACGTCCGGTAAATCCCAGTTTTTCCGTAGTAGTGGCTTTC  >  NC_000913/2870912‑2871394

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑