Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,732,992	G→A	I394I (ATC→ATT)  I394I (ATC→ATT)	clpB ← clpB ←	chaperone protein ClpB chaperone protein ClpB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,732,992	0	G	A	97.0%	102.0 / NA	33	I394I (ATC→ATT)  I394I (ATC→ATT)	clpB clpB	chaperone protein ClpB chaperone protein ClpB
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (17/15);  minor base C (1/0);  total (18/15)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTAATGGTCTGCGTACCAGAAAGCGATGCCTTCTCTGCTTTCCACTCTTCTTCTAACTCGGAGTACTGACGTTCTTTGTCGCTCAGTTCTTCGTTGAGCATATCCAGACGTTTTTTACTGGCTTCATCAGACTCTTTCATTAACGCCTGTTGTTCCAGTTTGAGCTGGATGATACGACGATCGAGTCGGTCGAGTTCTTCTGGTTTTGAGTCAATCTGCATACGAATGCTGGATGCTGCTTCATCGATCAGGTCGATGGCTTTATCCGGCAGCTGACGGTCAGCAATGTAGCGATGAGACAACGTCGCCGCTGCAACAATTGCCGGGTCAGTAATTTGCACATGGTGGTGCAATTCGTAACGTTCTTTCAGGCCACGCAG‑AATCGCAATGGTATCTTCAACAGAAGGCTCGGCAACAAACACTTTCTGGAAACGACGTTCCAGCGCAGCATCTTTTTCAATGTACTGGCGATATTCGTCAAGCGTC  >  NC_000913/2732747‑2733232                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                  ttAATGGTCTGCGTACCAGAAAGCGATGCCTTCTCTGCTTTCCACTCTTCTTCTAACTCGGAGTACTGACGTTCTTTGTCGCTCAGTTCTTCGTTGAGCATATCCAGACGTTTTTTACTGGCTTCATCAGACTCTTTCATTAACGCCTGTTGTTCCAGTTTGAGCTGGATGATACGACGATCGAGTCGGTCGAGTTCTTCTGGTTTTGAGTCAATCTGCATACGAATGCTGGATGCTGCTTCatcaatca                                                                                                                                                                                                                                               <  1:223392/250‑1 (MQ=255)     tGGTCTGCGTACCAGAAAGCGATGCCTTCTCTGCTTTCCACTCTTCTTCTAACTCGGAGTACTGACGTTCTTTGTCGCTCAGTTCTTCGTTGAGCATATCCAGACGTTTTTTACTGGCTTCATCAGACTCTTTCATTAACGCCTGTTGTTCCAGTTTGAGCTGGATGATACGAAGATCGAGTCGGTCGAGTTCTTCTGGTTTTGAGTCAATCTGCATACGAATGCTGGATGCTGCTTCATCAATCAGGTCg                                                                                                                                                                                                                                          >  1:168924/1‑251 (MQ=255)      ggTCTGCGTACCAGAAAGCGATGCCTTCTCTGCTTTCCACTCTTCTTCTAACTCGGAGTACTGACGTTCTTTGTCGCTCAGTTCTTCGTTGAGCATATCCAGACGTTTTTTACTGGCTTCATCAGACTCTTTCATTAACGCCTGTTGTTCCAGTTTGAGCTGGATGATACGACGATCGAGTCGGTCGAGTTCTGCTGGTTTTGAGTCAATCTGCATACGAATGCTGGATGCTGCTTCATCAATCAGGTCg                                                                                                                                                                                                                                          <  1:47214/250‑1 (MQ=255)            cGTACCAGAAAGCGATGCCTTCTCTGCTTTCCACTCTTCTTCTAACTCGTAGTACTGACGTTCTTTGTCGCTCAGTTCTTCGTTGAGCATATCCAGACGTTTTTTACTGGCTTCATCAGACTCTTTCATGAACGCCTGTTGTTCCAGTTTGAGCTGGATGATACGACGATCGAGTCGGTCGAGTTCTTCTGGTTTTGAGTCAATCTGCATACGAATGCTGGATGCTGCTTCATCAATCAGGTCGATGGCtt                                                                                                                                                                                                                                   <  2:76444/251‑1 (MQ=255)               aCCAGAAAGCGATGCCTTCTCTGCTTTCCACTCTTCTTCTAACTCGGAGCACTGACGTTCTTTGTCGCTCAGTTCTTCGTTGAGCATAGCCAGACGTTTTTTACTGGCTTCATCAGACTCTTTCATTAACGCCTGTTGTTCCAGTTTGAGCTGGATGATACGACGATCGAGTCGGTCGAGTTCTTCTGGTTTTGAGTCAATCTGCATACGAATGCTGGATGCTGCTTCATCAATCAGGTCGATGGCTTTAt                                                                                                                                                                                                                                <  1:204934/251‑1 (MQ=255)                   gAAAGCGATGCCTTCTCTACGTTCCACTCTTCTTCTAACTCGGAGTACTGACGTTCTTTGTCGCTCAGTTCTTCGTTGAGCATATCCAGACGTTTTTTACTGGCTTCATCAGACTCTTTCATTAACGCCTGTTGTTCCAGTTTGAGCTGGATGATACGACGATCGAGTCGGTCGAGTTCTTCTGGTTTTGAGTCAATCTGCATACGAATGCTGGATGCTGCTTCATCAATCAGGTCGATGGCTTTATCCgg                                                                                                                                                                                                                            >  1:236287/1‑251 (MQ=255)                                                    tctAACTCGGAGGACTGACGTTCTTTGTCGCTCAGTTCTTCGTTGAGCATATCCAGACGTTTTTTACTGGATTCATCAGACTCTTTCATTAACGCCTGTTGTTCCAGTTTGAGCTGGATGATAAGACGATCGAGTCGGTCGAGTTCTTCTGGTTTTGAGTCAATCTGCATACGAATGCTGGATGCTGCTTCATCAATCAGGTCGATGGCTTTATCCGGCAGCTGCCGGTCAGCAATGTAGCGATGAGACa                                                                                                                                                                                            >  1:115011/1‑250 (MQ=255)                                                         cTCGGAGTACTGACGTTCTTTGTCGCCCAGTTCTTCGTTGAGCATATCCAGACGTTTTTTACTGGCTTCATCAGCCTCTTTCATTCCAGCCTGTTGTTCCAGTTTGAGCTGGATGATACGACGATCGCGTCGGTCGAGTTCTTCTGGTTTTGAGTCAATCTGCATACGAATGCTGGATGCTGCTTCATCAATCAGGTAGATGGCTTTATCCGGCCGCTGACGGACACCAATGTCTCGTTGAGACAACGTcg                                                                                                                                                                                      >  2:135221/1‑251 (MQ=255)                                                                       gTTCTTTGTCGCTCAGTTCTTCGTTGAGCATATCCAGACGTTTTTTACTGGCTTCATCAGACTCTTTCATTAACGCCTGTTGTTCCAGTTTGAGCTGGATGATACGACGATCGAGTCGGTCGAGTTCTTCTGGTTTTGAGTCAATCTGCATACGAATGCTGGATGCTGCTTCATCAATCAGGTCGATGGCTTTATCCGGCAGCTGACGGTCAGCACTGTAGCGATGAGACAACGTCGCCGCTGCAACAAtt                                                                                                                                                                        >  2:271995/1‑251 (MQ=255)                                                                       gTTCTTTGTCGCTCAGTTCTTCGTTGAGCATATCCAGACGTTGTTTACTGGCTTCATCAGACTCTTTCATTAACGCCTGTTGTTCCAGTTTGAGCTGGATGATACGACGATCGAGTCGGTCGAGTTCTTCTGGTTTTGAGTCAATCTGCATACGAACGCTGGATGCTGCTTCATCAATCAGGTCGATGGCTTTATCCGGCAGCTGACGGTCAGCAATGTAGCGATTAGACAACGTCGCCGCTGCAACAAtt                                                                                                                                                                        <  2:24676/251‑1 (MQ=255)                                                                       gTTCTTTGTCGCTCAGTTCTTCGTTGAGCATAGCCAGACGGTTTTTACTGGCTTCATCAGACTCTTTCATTAACGCCTGTTGTTCCAGTTTGAGCTGGATGATACGACGATCGAGTCGGTCGAGTTCTTCTGGTTTTGAGTCAATCTGCATACGAATGCTGGATGCTGCTTCATCAATCAGGTCGATGGCTTTATCCGGCATCTGACGGTCAGCAATGTAGCGATGAGACAACGTCGCCGCTGCAACAAtt                                                                                                                                                                        <  2:52083/251‑1 (MQ=255)                                                                                     aGTTCTTCGTTGAGCATATCCAGACGTTTTTTACTGGCTTCATCAGACTCTTTCATTAACGCCTGTTGTTCCAGTTTGAGCTGGATGATACGACGATCGCGTCGGTCGAGTTCTTCTGGTTTTGAGTCAATCTGCATACGAATGCTGGATGCTGCTTCATCAATCAGGTCGATGGCTTTATCCGGCAGCTGACGGTCAGCAATGTAGCGATGAGACAACGTCGCCGCTGCAACACTTGCCGGGTCAGTAAt                                                                                                                                                          >  1:74320/1‑251 (MQ=255)                                                                                        tcttcGTTGAGCATATCCAGACGTTTTTTACTGGCTTCATCAGACTCTTTCATTAACGCCTGTTGTTCCAGTTTGAGCGGGATGATACGACGATCGAGTCGGTCGAGTTCTTCTGGTTTTGAGTCAATCTTCATACGAATGCTGGATGCTGCTTCATCAATCAGGTCGAGGGCTTGATCCGGCAGCTGACGGTCATCAATGTAGCGATGATACAACGTCGCCGCTGCAACAATTGCCGGGTCAGTAAttt                                                                                                                                                        <  2:38008/250‑1 (MQ=255)                                                                                               tgcgCATATTCAGACGCATTTAACTGGCTCCATCCCACTCTTTTATTAACGCATGTTGTTCCAGTTTGAGCTGGATGATACGACGATCGAGACTGACGAGTTCTTCTGGTTTTGAGTCAATCTGCATACGAATGCTAGATGCTGCTTCATCCATCCGGTCGATGGCTTTCTCCGGCAGCTGACGGTCCGCAATGTAGCGATGAGACAACGTCGCCGCAGCAACCACTGCCTGTACATTAATTTGCACAtgg                                                                                                                                                >  2:24087/4‑251 (MQ=255)                                                                                                gAGCATATCCAGACGTTTTTTACTGGCTTCATCAGACTCTTTCATTAACGCCTGTTGTTCCAGTTTGAGCTGGATGATACGACGATCGAGTCGGTCGAGTTCTTCTGGTTTTGAGTCAATCTGCATACGAATGCTGGATGCTGCTTCATCAATCAGGTCGATGGCTTTATCCGGCAGCTGACGGTCAGCAATGTAGCGATGAGACAACGTCGCCGCTGCAACAATTGCCGGGTCAGTAATTTGCACAtggt                                                                                                                                               <  1:271995/251‑1 (MQ=255)                                                                                                                     aCTGGCTTCATCAGACTCTTCCATTAACGCCTGTTGTTCCAGTTTGAGCTGGATGATACGACGATCGAGTCGGTCGAGTTCTTCTGGTTTTGCGTCAATCTGCATACGAATGCTGGATGCTGCTTCATCAATCAGGTCGATGGCTTTATCCGGCAGCTGACGGTCAGCAATGTAGCGATGAGACAACGTCGCCGCTGCAACAATTGCCGGGTCCGTAATTTGCACATGGTGGTGCAATTCGTACCGTTCtt                                                                                                                          >  1:201900/1‑251 (MQ=255)                                                                                                                                      ctTTCATTAACGACTGACGTTCCAGCTTGAGCTGGATGATACGACGATCGAATCGGTCGAGTTCTTCTGGTTTTGGGTCAATCTGCATACGAATGCTGGATGCTGCTTCATCAATCAGGTCGATGGCTTTATCCGGCAGCTGCCGGTCAGCAATGTAGCGATGAGACAACGTCGCCGCTGCAACAATTGCCGGGTCAGTAATTTGCACATGGTGGTGCAATTCCTAACGTTCTTTCAGGCCACGCCG‑AATc                                                                                                        >  2:156664/1‑251 (MQ=255)                                                                                                                                        ttCATTAACGCCTGTTGTTCCAGTTTGAGCTGGATGATACGACGATCGAGTCGGTCGAGTTCTTCTGGTTTTGAGTCAATCTGCATACGAATGCTGGATGCTGCTTCATCAATCAGGTCGATGGCTTTATCCGGCAGCTGACGGTCAGCAATGTAGCGATGAGACAACGTCGCCGCTGCAACAATTGCCGGGTCAGTAATTTGCACATGGTGGTGCAATTCGTAACGTTCTTTCAGGCCACGCAG‑AAt                                                                                                         <  1:145947/248‑1 (MQ=255)                                                                                                                                          cATTAACCCATGTTGTTCCGGTTTGACCTGGATGATACGACGATCGAGTCGCTCGAGTTCTTCTGGATTTGAGACAATATGCCTACGAATCCTGGATGCTGCTTCATCAATCAGGTCGATGGCTTTATCCGGCAGCTGACGGTCAGCAATATAGCGATGAGACAACGTCGCCGCTGCAACAATTGCACGGTCAGTAATTTGCACATGGTGGTGCAATTCGTAACGATCTTACAGGCCACGCAG‑AATCGCa                                                                                                     >  2:49052/1‑250 (MQ=255)                                                                                                                                              aaCGCCTGTTGTTCCAGTGTGATCTGTATGATATGACGATCTAGTCTGTCGTGTTCTTCTGGGTTTGAGGCAATCTTCATACGAATGCTGTATGCTGCTTCATCAATCAGGTCTATGGCTTTATCCGGCATCTGACGGTCAGCAATGTAGCGATGAGACAACGTCGCCGCTGCAACAACTGCTGGGTCATTAATTGGCGTATGGTTGTGCAATTCGTAACGTTCTTGCAGGGCACGCAG‑AATCGCAATGGt                                                                                                <  2:20904/251‑1 (MQ=255)                                                                                                                                                       tgttCCAGTTTGAGCTGGATGATACGACGATCGAGTCGGTCGAGTTCTTCTGGTTTTGAGTCAATCTGCATACGAATGCTGGATGCTGCTTCATCAATCAGGTCGATGGCTTTATCCGGCAGCTGACGGTCAGCAATGTAGCGATGAGACAACGTCGCCGCTGCAACAATTGCCGGGTCAGTAATTTGCACATGGTGGTGCAATTCGTAACGTTCTTTCAGGCCACGCAG‑ACTCGCAATGGTATCTTCac                                                                                        >  2:169437/1‑249 (MQ=255)                                                                                                                                                               tttGAGCTGGATGATACGACGATCGAGTCGGTCGAGTTCTTCTGGATTTGAGTCAATATGCATACGAATGCTGGATGCTGCTTCCTCAATCAGGTCGATGGCTTTATCCGGCAGCTGACGGTCAGCAATGTAGCGATGCGACAACGTCGCCGCTGCAACAATTGCCGGGTCAGTAATTTGCACATGGGGGTGCAATTCGTAACGTTCTTTCAGGCCACGCAG‑AATCGCAATGGAATCTTCAACAGAAGGCt                                                                               >  2:156537/1‑251 (MQ=255)                                                                                                                                                                              acgacgATCGAGTCGGTCGAGTTCTTCTGGTTTTGAGTCAATCTGCATACGAATGCTGGATGCTGCTTCATCAATCAGGTCGATGGCTTTATCCGGCAGCTGACGGTCAGCAATGTAGCGATGAGACAACGTCGCCGCTGCAACAATTGCCGGGTCAGTAATTTGCACATGGTGGTGCAATTCGTAACGTTCTTTCAGGCCACGCAG‑AATCGCAATGGTATCTTCAACAGAAGGCTCGGCAACAAACACt                                                                 <  1:100807/250‑1 (MQ=255)                                                                                                                                                                               cgacgaTCGAGTCGGTCGAGTTCTTCTGGTTTTGAGTCAATCTGCATACGAATGCTGGATGCTGCTTCATCAATCAGGTCGATGGCTTTATCCGGCAGCTGACGGTCAGCAATGTAGCGATGAGACAACGTCGCCGCTGCAACAATTGCCGGGTCAGTAATTTGCACATGGTGGTGCAATTCGTAACGTTCTTTCAGGCCACGCAG‑AATCGCAATGGTATCTTCAACAGAAGGCTCGGCAACAAACACttt                                                               <  2:58120/251‑1 (MQ=255)                                                                                                                                                                                 aagaTCGAGTCGGTCGAGTTCTTCTGGTTTTGAGTCAATCTGCATACGAATGGTGGATGCTGCTTCATCAATCAGGTCGATGGCTTTATCCGGCAGCTGACGGTCAGCAATGTAGCGATGAGACAACGTCGCCGCTGCAACAATTGCCGGGTCCGTAATTTGCACATGGTGGTGCAATTCGTCACGTTCTTTCAGGCCACGCAG‑AATCGCAATGGCATCTTCAACAGAAGGCTCTGCCACAAACACTTTCt                                                             >  2:113674/3‑251 (MQ=255)                                                                                                                                                                                                  gTTCTTCTGGTTTTGAGTCAATCTGCATACGAATGCTGGATGCTGCTTCATCAATCAGGTCGATGGCTTTATCCGGCAGCTGACGGTCAGCAATGTAGCGATGAGACAACGTCGCCGCTGCAACAATTGCCGGGTCAGTAATTTGCACATGGTGGTGCAATTCGTAACGTTCTTTCAGGCCACGCAG‑AATCGCAATGGTATCTTCAACAGAAGGCTCGGCAACAAACACTTTCTGGAAACGACGTTCCAgc                                            >  1:25801/1‑251 (MQ=255)                                                                                                                                                                                                   ttcttcTGGTTTTGAGTCAATCTGCATACGAATGCTGGATGCTGCTTCATCAATCAGGTCGATGGCTTTATCCGGCAGCTGACGGTCAGCAATGTAGCGATGAGACAACGTCGCCGCTGCAACAATTGCCGGGTCAGTAATTTGCACATGGTGGTGCAATTCGTAACGTTCTTTCAGGCCACGCAG‑AATCGCAATGGTATCTTCAACAGAAGGCTCGGCAACAAACACTTTCTGGAAACGACGTTCCAgcg                                           >  1:225590/1‑251 (MQ=255)                                                                                                                                                                                                                gAGTCAATCTGCATACGAATGCTGGATGCTGCTTCATCAATCAGGTCGATGGCTTTATCCGGCAGCTGACGGTCAGCAATGTAGCGATGAGACAACGTCGCCGCTGCAACAATTGCCTGGTCAGTAATTTGCACATGGTGGTGCAATTCGTAACGTTCTTTCAGGCCACGCAG‑AATCGCAATGGTATCTTCAACAGAAGGCTCGGCAACAAACACTTTCTGGAAACGACGTTCCAGCGCAGCATCttttt                               <  2:236287/250‑1 (MQ=255)                                                                                                                                                                                                                    cAATCTGCATACGAATGCTGGATGCTGCTTCATCAATCAGGTCGATGGCTTTATCCGGCAGCTGACGGTCAGCAATGTAGCGATGAGACAACGTCGCCGCTGCAACAATTGCCGGGTCAGTAATTTGCACATGGTGGTGCAATTCGTAACGTTCTTTCAGGCCACGCAG‑AATCGCAATGGTATCTTCAACAGAAGGCTCGGCAACAAACACTTTCTGGAAACGACGTTCCAGCGCAGCATCTTTTTCAATg                          <  1:38075/251‑1 (MQ=255)                                                                                                                                                                                                                     aaTCTGCATACGAATGCTGGATGCTGCTTCATCAATCAGGTCGATGGCTTTATCCGGCAGCTGACGGTCAGCAATGTAGCGATGAGACAACGTCGCCGCTGCAACAATTGCCGGGTCAGTAATTTGCACATGGTGGTGCAATTCGTAACGTTCTTTCAGGCCACGCAG‑AATCGCAATGGTATCTTCAACAGAAGGCTCGGCAACAAACACTTTCTGGAAACGACGTTCCAGCGCAGCATCTTTTTCAATGt                         >  1:148848/1‑251 (MQ=255)                                                                                                                                                                                                                      atcggCATACGAATGCTGGATGCGGCTTCATCAATCAGGTCGATGGCTTTATCCGGCAGCTGACGGTCAGCAATGTAGCGATGAGACAACGTCGCCGCTGCAACAATTGCCGGGTCAGTAATTTGCACATGGTGGTGCAAGTCTTAACGTTCGTTCATGCC‑CGTGGTGATCGCAATGGTATCTTCAACAGAAGGCTCGGCAACAAACACTGGCTGGAAACGACGGTCCAGCTCAGCATCTTTGTCAATGTa                        <  2:191368/247‑1 (MQ=255)                                                                                                                                                                                                                                       ggATGCTGCTTCATCAATCAGGTCGATGGCTTTATCCGGCAGCTGACGGTCAGCAATGTAGCGATGAGACAACGTCGCCGCTGCAACAATTGCCGGGTCAGTAATTTGCACATGGTGGTGCAATTCGTAACGTTCTTTCAGGCCACGCAG‑AATCGCAATGGTATCTTCAACAGAAGGCTCGGCAACAAACACTTTCTGGAAACGACGTTCCAGCGCAGCATCTTTTTCAATGTACTGGCGATATTCGTCaa       <  1:96465/251‑1 (MQ=255)                                                                                                                                                                                                                                       ggATGCTGCTTCATCAATCAGGTCGATGGCTTTATCCGGCAGCTGACGGTCAGCAATGTAGCGATGAGACAACGTCGCCGCTGCAACAATTGCCGGGTCAGTAATTTGCACATGGTGGTGCAATTCGTAACGTTCTTTCAGGCCACGCAG‑AATCGCAATGGTATCTTCAACAGAAGGCTCGGCAACAAACACTTTCTGGAAACGACGTTCCAGCGCAGCATCTTTTTCAATGTACTGGCGATATTCGTCa        >  1:213097/1‑250 (MQ=255)                                                                                                                                                                                                                                            ctgctTCATCAATCAGGTCGATGGCTTTATCCGGCAGCTGACGGTCAGCAATGTAGCGATGAGACAACGTCGCCGCTGCAACAATTGCCGGGTCAGTAATTTGCACATGGTGGTGCAATTCGTAACGTTCTTTCAGGCCACGCAG‑AATCGCAATGGTATCTTCAACAGAAGGCTCGGCAACAAACACTTTCTGGAAACGACGTTCCAGCGCAGCATCTTTTTCAATGTACTGGCGATATTCGTCAAGcgtc  >  1:256155/1‑251 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                  TTAATGGTCTGCGTACCAGAAAGCGATGCCTTCTCTGCTTTCCACTCTTCTTCTAACTCGGAGTACTGACGTTCTTTGTCGCTCAGTTCTTCGTTGAGCATATCCAGACGTTTTTTACTGGCTTCATCAGACTCTTTCATTAACGCCTGTTGTTCCAGTTTGAGCTGGATGATACGACGATCGAGTCGGTCGAGTTCTTCTGGTTTTGAGTCAATCTGCATACGAATGCTGGATGCTGCTTCATCGATCAGGTCGATGGCTTTATCCGGCAGCTGACGGTCAGCAATGTAGCGATGAGACAACGTCGCCGCTGCAACAATTGCCGGGTCAGTAATTTGCACATGGTGGTGCAATTCGTAACGTTCTTTCAGGCCACGCAG‑AATCGCAATGGTATCTTCAACAGAAGGCTCGGCAACAAACACTTTCTGGAAACGACGTTCCAGCGCAGCATCTTTTTCAATGTACTGGCGATATTCGTCAAGCGTC  >  NC_000913/2732747‑2733232

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑