Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,510,609	C→T	P327L (CCG→CTG)	yfeO →	putative transport protein YfeO

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,510,609	0	C	T	100.0%	53.6 / NA	21	P327L (CCG→CTG)	yfeO	putative transport protein YfeO
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (16/5);  total (16/5)

GGTTGCACGCGATGATGCATCAAATGAAAAATCCGGTGCTCGTGCTGGGTATTGGCGGATTTATTCTCGGTATTCTGGGGGTTATTGGTGGACCAGTTTCGCTGTTTAAAGGGCTGGATGAGATGCAGCAGATGGTGGCAAATCAGGCTTTCAGCACCAGCGATTACTTTTTGCTGGCGGTAATTAAACTTGCCGCCCTGGTCGTTGCTGCCGCCAGTGGCTTTCGCGGTGGGCGAATCTTCCC‑GGCAGTGTTTGTCGGCGTGGCATTAGGGTTGATGCTGCATGAGCACGTTCCCGCCGTACCAGCGGCAATAACCGTTTCTTGCGCTATTCTCGGCATCGTGCTGGTGGTAACACGCGATGGCTGGTTAAGTCTTTTTATGGCGGCAGTCGTTGTACCCAATACCACATTGCTACCGCTGCTCTGTATCGTCATGCTTCCGGCATGGCTGTTATTAG  >  NC_000913/2510366‑2510824                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                         ggTTGCACGCGATGATGCATCAAATGAAAAATCCGGTGCTCGTGCTGGGTATTGGCGGATTTATTCTCGGTATTCTGGGGGTTATTGGGGGACCAGTTTCGCTGTTTAAAGGGCTGGATGAGATGCAGCAGATGGTGGCAAATCAGGCTTTCAGCACCAGCGATTACTTTTTGCTGGCGGTAATTAAACTTGCCGCCCTGGTCGTTGCTGCCGCCAGTGGCTTTCGCGGTGGGCGAATCTTCCT‑GGCAgtg                                                                                                                                                                                                                  <  2:225190/251‑1 (MQ=255)         gcgATGATGCATCAAATGAAAAATCCGGTGCTCGTGCTGGGTATTGGCGGATTTATTCTCGGTATTCTGGGGGTTATTGGTGGACCAGTTTCGCTGTTTAAAGGGCTGGATGAGATGCAGCAGATGGTGGCAAATCAGGCTTTCAGCACCAGCGATTACTTTTTGCTGGCGGGAATTTAACTTGCCCCCCTGGTCGTTTCTGCCGCCAGTGGCTTTTGCGGGGGGCGGATCTTCCT‑TGCAGGGTTTTTCgg                                                                                                                                                                                                          >  1:225331/1‑251 (MQ=255)                   aTCAAATGAAAAAGCCGGTGCTCGTGCTGGGTATTGGCGGATTTATTCTCGGTATTCTGGGGGTTATTGGTGGACCAGTTTCGCTGTTTAAAGGGCTGGATGAGATGCAGCAGATGGTGGCAAATCAGGCTTTCAGCACCAGCGATTACTTTTTGCTGGCGGTAATTAAACTTGCCGCCCTGGTCGTTGCTGCCGCCAGTGGCTTTCGCGGTGGGCGAATCTTCCT‑GGCAGTGTTTGTCGGCGTGGCATTa                                                                                                                                                                                                <  1:307124/251‑1 (MQ=255)                     aaaaTGAGAAAGCCGGTGCTCGTGCTGGGTATTGGCGGTGTTATTCACGGTATTCTGGGGGTTATTGGTGGAATAGTTTGGCTGTTTAAAGGTCTGGTTGAGATGCAGCAGTTGGTGGTAAAGCAGGCTTTGAGCACCAGCGATTTCGTTTTGCGGGCGGTAATTAAGCTTGCCGCCCTGGTCGTTGCGGCCGCCAGTGGCTTTCGCGGTGGGCGAATCTTCCT‑GGCAGTGTTTGTCGGCGTGGCATTAgt                                                                                                                                                                                              <  1:305392/250‑2 (MQ=255)                          gAAAAATCCGGTGCTCGTGCTGGGTATTGGCGGATTTATTCTCGGTATTCTGGGGGTTATTGGTGGACCAGTTTCGCTGTTTAAAGGGCTGGATGAGATGCAGCAGATGGTGGCAAATCAGGCTTTCAGCACCAGCGATTACTTTTTGCTGGCGGTAATTAAACTTGCCGCCCTGGTCGTTGCTGCCGCCAGTGGCTTTCGCGGTGGGCGAATCTTCCT‑GGCAGTGTTTGTCGGCGTGGCATTAGGGTTGa                                                                                                                                                                                         <  1:130372/251‑1 (MQ=255)                           aaaaaTCCGGTGCTCGTGCTGGGTATTGGCGGATTTATTCTCGGTATTCTGGGGGTTATTGGTGGACCAGTTTCGCTGTTTAAAGGGCTGGATGAGATGCAGCAGATGGTGGCAAATCAGGATTTCAGCACCAGCGATTACTTTTTGCTGGCGGTAATTAAACTTGCCGCCCTGGGCGTTGCTGCCGCCAATGGGTTTCGCGGGGGGCGCATCTTTCT‑GGCAGTGTTTGTCGGCGTGGCGTTATGGGTGAt                                                                                                                                                                                        >  1:80722/1‑251 (MQ=255)                                         cGTGCTGGGTATTGGCGGATTTATTCTCGGTATTCTGGGGGTTATTGGTGGACCAGTTTCGCTGTTTAAAGGGCTGGATGAGATGCAGCAGATGGTGGCAAATCAGGCTTTCAGCACCAGCGATTACTTTTTGCTGGCGGTAATTAAACTTGCCGCCCTGGTCGTTGCTGCCGCCAGTGGCTTTCGCGGTGGGCGAATCTTCCT‑GGCAGTGTTTGTCGGCGTGGCATTAGGGTTGATGCTGCATGAGCAc                                                                                                                                                                           <  1:231724/250‑1 (MQ=255)                                                               aTTCTCGGAATTCTGGGGGTTATTGGTGGACCAGGTTCGCTGTTTAAAGGGCTGGATGAGATGCAGCAGATGGTGGCAAATCAGGCTTTCAGCACCAGCGATTACTTTTTGCTGGCGGTAATTAAACTTACCGCCCAGGTCGTTGCTGCCGTCAGTGGCCTTCGCGGGGGGCGCATATTCCT‑GGCAGGGTTTGTCGGGGTGGAATTAAGGTTGATACCGCATGAGCAAGCTACCGCAGTACCAGCGGAAAt                                                                                                                                                    >  1:309020/1‑251 (MQ=255)                                                                  ctcGGTATTCTGGGGGTTATTGGTGGACCAGTTTCGCTGTTTAAAGGGCTGGATGAGATGCAGCAGATGGTGGCAAATCAGGCTTTCAGCACCAGCGATTACTTTTTGCTGGCGGTAATTAAACTTTCCGCCCTGGTCGTTGGTGCCGCCAGGGGCTTTCGCGGTGGGCGACAATTCCTTGTCGGTGTT‑GTCGGCGTGGCATTAGGGTTGATGCTGTCTGCGCACGTTTCCTCCGTACCAGCGGCAATAAc                                                                                                                                                 >  1:269241/1‑251 (MQ=255)                                                                                        gtggACCAGTTTCGCTGTTTCAAGGGCTGGATGAGATGCAGCAGATGGTGGCAAATCAGGCTTTCAGCACCAGCGATTACTTTTTGCTGGCGGTAATTAAACTTGCCGCCCTGGTCGTTGCTGCCGCCCGTGGCTTTCGCGGGGGGCGAATCTTCCT‑GGCCGGGTTTGTTGGCGTGGCATTAGGGGTGATGCTGCATGAGCACGTTCCCGCCGTACCAGCGGCAAAAACCGTTTCTTGCTCTATTCCCGGc                                                                                                                           >  2:37504/1‑251 (MQ=255)                                                                                          ggACCAGTTTCGCTGTTTAAAGGGATGGATGAGATGCAGCAGATGGTGGCAAATCAGGCTTTCAGCACCAGCGATTACTTTTTGCTGGCGGTAATTAAACTTGCCGCCCTGGTCGTTGCTGCCGCCAGTGGCTTTCGCGGAGGGCGAATCTTCCT‑GGCAGTGTTTGTCGGCGTGGCATTAGGGTTGATGCTGCATGAGCGCGTTCCCGCCGTCACGGCGGCACTAACCGTTTCTTTCGCTCTTTTCGGCac                                                                                                                         >  2:33458/1‑250 (MQ=255)                                                                                             ccAGTTTCGCTGTTTAAAGGGCTGGATGAGATGCAGCAGATGGTGGCAAATCAGGCTTTCAGCACCAGCGATTACTTTTTGCTGGCGGTAATTAAACTTGCCGCCCTGGTCGTTGCTGCCGCCAGTGGCTTTCGCGGTGGGCGAATCTTCCT‑GGCAGTGTTTGTCGGCGTGGCATTAGGGGTGATGCTGCATGGGCCCGTTCCCGCCGTACCAGCGGCAATAACCGTTTCTTGCGCTATTCTCGGCATCGt                                                                                                                      >  1:54190/1‑251 (MQ=255)                                                                                                       tGTTTAAGGGTCTGGATGAGATGCAGCAGATGGTGGCAAATCAGGCTTTCAGCACAAGCGATTACTTTTTGATGGCGGTAATTAAACTTTCCGCCCTGGTCGCTGATGCCGCCAGTGGCTTTCGCGGTGGGCGAATCTTCCT‑GGCAGTGTTTGTCGGCGTGGCATTAGGGTTGTTGCTGCATGAGCAAGTTCCCGCCGCACCAGCGGCAATAACCGTTTCTTGCGCTATTCTCGGCCTCGTGCtggtggg                                                                                                             >  2:205394/1‑249 (MQ=255)                                                                                                                   tGGATGAGATGCAGCAGATGGTGGCAAATCAGGCTTTCAGCACCAGCGATTACTTTTTGCTGGCGGTAATTAAACTTGCCGCCCTGGTCGTTGCTGCCGCCAGTGGCTTTCGCGGTGGGCGAATCTTCCT‑GGCAGTGTTTGTCGGCGTGGCATTAGGGTTGATGCTGCATGAGCACGTTCCCGCCGTACCAGCGGCAATAACCGTTTCTTGCGCTATTCTCGGCATCGTGCTGGTGGTAACACGCGATGGc                                                                                                >  1:47519/1‑251 (MQ=255)                                                                                                                          gaTGCAGCAGATGGTGGCAAATCAGGCTTTCAGCACCAGCGATTACTTTTTGCTGGCGGAAATTAAACTTTCCGCCCTGGTCGTTTCTGCCGCCCGTGGCTTTAGCGGTGGGGGAATCTTCCT‑GGGAGTGTTTGTCGGCGTGGCAATTGGGTTGATGCTGCATGAGCCAGTTTCCCCCGTAGCAGCGGCCATAACCGTTTCTTGCGCCATTCTCCGCATCGTGCTGGGGGTGAAACGACATGGCTGGttt                                                                                          >  2:177260/1‑249 (MQ=255)                                                                                                                           aTGCAGCAGATGGTGGCAAATCAGGCTTTAAGCACCAGCGATTACTTTTTGCTGGCGGTAATTAAACTTGCCGCCCTGGGCGTTGCTGCAGCCCGGGGCTTTCGCGGGGGGGGGATCTTCCT‑TGCAGTGTTTGTCGGCGTGGCATTTGGGGTGGTGGTGGCTGAGCACGTTCCCGCCGTACCAGCGGCAATAACCGTTTCTTGCGCTATTCTCGGCATCGTGCTGGGGGGAacac                                                                                                        >  2:157008/1‑235 (MQ=255)                                                                                                                                 cagATGGTGGCAAATCAGGCTTTCAGCACCAGCGATTACTTTTTGCTGGCGGTAATTAAACTTGCCGCCCTGGGCGTTGCTGCCGCCAGTGGCTTTCGCGGTGGGCGAATCTTCCT‑GGCAGTGTTTGTCGGCGTGGCATTAGGGTTGGTGCTGCATGAGCACGTTCCCGCCGTACCAGCGGCCATAAACGTTTCTTGCGCt                                                                                                                                    >  2:263066/1‑201 (MQ=255)                                                                                                                                           cAAATCAGGCTTTCAGCACCAGCGATTACTTTTTGCTGGCGGTCATTAAACTTGCCGCCCTGGTCGTTGCTGCCGCCCGTGGCTTTCGCGGTGGGCGAATCTTCCT‑GGGAGTGTTTGTCGGCGGGGCATTTGGGGTGGTGCTGCATGAGCACGTTCCCGCCGTACCCGCGGCAATAACCGTTTCTTGCGCTATTCTCGGCCTCGTGCTGGTGGTAACACGCGATGGCTGGTTAAGTCTTTTTATggcgggg                                                                        >  2:152157/1‑249 (MQ=255)                                                                                                                                                                                     tAATTAAACTTGCCGCCCTGGTCGTTGCTGCCGCCAGTGGCTTTCGCGGTGGGCGAATCTTCCT‑GGCAGTGTTTGTCGGCGTGGCATTAGGGTTGATGCTGCATGAGCACGTTCCCGCCGTACCAGCGGCAATAACCGTTTCTTGCGCTATTCTCGGCATCGTGCTGGTGGTAACACGCGATGGCTGGTTAAGTCTTTTTATGGCGGCAGTCGTTGTACCCAATACCACATTGCTACCGCTGCTCTGTATc                              >  1:298399/1‑251 (MQ=255)                                                                                                                                                                                                                 tgcCGCCAGTGGCTTTCGCGGTGGGCGAATCTTCCT‑GGCAGTGTTTGTCGGCGTGGCATTAGGGTTGATGCTGCATGAGCACGTTCCCGCCGTACCAGCGGCAATAACCGTTTCTTGCGCTATTCTCGGCATCGTGCTGGTGGTAACACGCGATGGCTGGTTAAGTCTTTTTATGGCGGCAGTCGTTGTACCCAATACCACATTGCTACCGCTGCTCTGTATCGTCATGCTTCCGGCATGGCTGttatttg  >  1:144834/1‑249 (MQ=255)                                                                                                                                                                                                                          tGGCTTTCGCGGTGGGCGAATCTTCCT‑GGCAGTGTTTGTCGGCGTGGCATTAGGGTTGATGCTGCATGAGCACGTTCCCGCCGTACCAGCGGCAATAACCGTTTCTTGCGCTATTCTCGGCATCGTGCTGGTGGTAACACGCGATGGCTGGtt                                                                                           >  1:275916/1‑153 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                         GGTTGCACGCGATGATGCATCAAATGAAAAATCCGGTGCTCGTGCTGGGTATTGGCGGATTTATTCTCGGTATTCTGGGGGTTATTGGTGGACCAGTTTCGCTGTTTAAAGGGCTGGATGAGATGCAGCAGATGGTGGCAAATCAGGCTTTCAGCACCAGCGATTACTTTTTGCTGGCGGTAATTAAACTTGCCGCCCTGGTCGTTGCTGCCGCCAGTGGCTTTCGCGGTGGGCGAATCTTCCC‑GGCAGTGTTTGTCGGCGTGGCATTAGGGTTGATGCTGCATGAGCACGTTCCCGCCGTACCAGCGGCAATAACCGTTTCTTGCGCTATTCTCGGCATCGTGCTGGTGGTAACACGCGATGGCTGGTTAAGTCTTTTTATGGCGGCAGTCGTTGTACCCAATACCACATTGCTACCGCTGCTCTGTATCGTCATGCTTCCGGCATGGCTGTTATTAG  >  NC_000913/2510366‑2510824

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑