Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,484,827	C→T	L152F (CTT→TTT)	evgS →	sensor histidine kinase EvgS

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,484,827	0	C	T	100.0%	41.5 / NA	15	L152F (CTT→TTT)	evgS	sensor histidine kinase EvgS
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (6/9);  total (6/9)

CGCAGCAACGGGTTCGTGGTATTAATGCTGATTATTTAAATCTTTTAAAAAGAGCGTTAAATATCAAATTAACACTCCGGGAATACGCAGATCATCAAAAAGCAATGGACGCGCTTGCAGAAGGTGAAGTCGATATAGTGTTATCACATTTAGTTACTTCGCCGCCTCTTAATAATGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTGGTAGTAACATCATTACCAGCAGTATGATTTCCCGCTATTTCACTCACTCCTTAAATGTAGTGAAATATT  >  NC_000913/2484597‑2485073                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                       cGCAGCAACGGGTTCGTGGTATTAATGCTGATTATTTAAATCTTTTAAAAAGAGCGTTAAATATCAAATTAACACTCCGGGAATACGCAGATCATCAAAAAGCAATGGACGCGCTTGCGGAAGGTGAAGTCGATATAGTGTTATCACATTTAGTTACTTCGCCGCCTCTTAATAATGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCTTTCACGACTCAATGCGACCg                                                                                                                                                                                                                                    >  1:216543/1‑251 (MQ=255)           ggTTCGTGGTATTAATGCTGATTATTTAAATCTTTTAAAAAGAGCGTTAAATATCAAATTAACACTCCGGGAATACGCAGATCATCAAAAAGCAATGGACGCGCTTGCAGAAGGTGAAGTCGATATAGTGTTATCACATTTAGTTACTTCGCCGCCTCTTAATAATGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCTTTCACGACTCAATGCGACCGCTTACCTCAc                                                                                                                                                                                                                          <  1:124949/251‑1 (MQ=255)                gTGGTATTAATGCTGATTATTTAAATCTTTTAAAAAGAGCGTTAAATATCAAATTAACACTCCGGGAATACGCAGATCATCAAAAAGCAATGGACGCGCTTGCAGAAGGTGAAGTCGATATAGTGTTATCACATTTAGTTACTTCGCCGCCTCTTAATAATGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCTTTCACGACTCAATGCGACCGCTTACCTCACCaaaa                                                                                                                                                                                                                     <  2:5823/251‑1 (MQ=255)                                      aaaTCTTTTAAAAAGAGCGTTAAATATCAAATTAACACTCCGGGAATACGCAGATCACCAAAAAGCAATGGACGCGCTTGCAGAAGGTGAAGTCGATATAGTGTTATCACATTTAGTTACATCGCCGCCTCTTAATAATGACATTGCGGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCTTTCACGACTCAATGCGACCGCTTTCCTCACCAAAACCAGTAAATATTGCTCGGGTa                                                                                                                                                                                                <  1:270819/250‑1 (MQ=255)                                              taataaGAGCGTTAAATATCAACTTAACACTCCGGGAATACTCAGATCATCAAAAAGCAATGGACGCGCTTGCAGAAGGTGAAGTCGATATAGTGTTATCACATTTAGTTACTTCGCCGCCTCTTAATAATGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCTTTCACGACTCAATGCGACCGCTTACCTCACCAAACACAGTAAATATTGCTCGGGTAGCAACTTAc                                                                                                                                                                                       >  2:54028/5‑251 (MQ=255)                                                 gaattgCGTTAAATATCAAATTAACACTCCGGGAATACACATATCATCAAAAAGCAATGGACGCGCTTGCAGAAGGTGAAGACGATATAGTGTTATCACATTTAGTTCCTTCGCCGCCTCTTAATAATGACATTGCTGCCACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCTTTCACGACTCAATGCGACCGCTTACCTCACCACAACCAGCAAATATTGCTCGGGTAGCAAATTAcccc                                                                                                                                                                                    >  2:121328/6‑251 (MQ=255)                                                           aaaTATCAAATTAACACTCCGGGAATACGCAGATCATCAAAAAGCAATGGACGCGCTTGCAGAAGGTGAAGTCGATAGAGTGTTATCACATTGAGTTTCTTCGCCGCCTCGTAATAATGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTTGTAACCACCTTTCACGACTCAATGCGACCGCTTACCTCACCAAATCCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCAGACGAgg                                                                                                                                                                          <  2:66120/251‑1 (MQ=255)                                                                          acTCCGGGAATACGCAGAGCATCAAAAAGCAATGGACGCGCTTGCAGAAGGTGAAGTCGATATAGTGTTATCACATTTAGTTACTTCGCCGCCTCTTAATAATGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCTTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCAGACGAGGTAATTCATCAATCAt                                                                                                                                                           <  2:210549/251‑1 (MQ=255)                                                                             ccGGGAATACGCAGATCATCAAAAAGCAATGGACGCGCTTGCAGAAGGTGAAGTCGATATAGTGTTATCACATTTAGTTACTTCGCCGCCTCTTAATAATGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCTTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCAGACGAGGTAATTCATCAATCAttt                                                                                                                                                         <  1:3631/250‑1 (MQ=255)                                                                               gggAATACGCAGATCATCAAAAAGCAATGGACGCGCTTGCAGAAGGTGAAGTCGATATAGTGTTATCACATTTAGTTACTTCGCCGCCTCTTAATAATGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCTTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGGAGCAAATTACCCCCCAGACGAGGTAATTCATCCATCATTTcc                                                                                                                                                       >  1:101907/1‑250 (MQ=255)                                                                                                             aCGCGCGTGCAGAAGGTGAAGTCGATATAGTGTTATCACATTTAGTTACTTCGCCGCCTCTTAATAATGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCTGCGCTGGTAACCACCTTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATGGCTCGGTTAGCAAATTACCCCCCAGACGAGGTAATTCATCAATCATTGCCAAAAGCGACAATTATCTCTTTTACAAATTta                                                                                                                        <  2:306224/251‑1 (MQ=255)                                                                                                                         aaGGTGAAGTCGATATAGTGTTATCACATTTAGTTACTTCGCCGCCTCTTAATAATGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCTTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCAtt                                                                                                             >  1:82318/1‑250 (MQ=255)                                                                                                                                                            aCTTCGCCGCCTCTTAATAATGACAGTGCTGCAACCAAACCATTGATAATTTCCTTTCCGGCGCTGGTAACCACCTTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCAGTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTa                                                                          <  2:38164/250‑1 (MQ=255)                                                                                                                                                                                                                       ggCGCTGGTAACCACCTTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTGGTAGTAACATCATTACCAGCAGTATGATTTCCCGCTATTTCACTCACTCCTTAAATg              <  2:82318/251‑1 (MQ=255)                                                                                                                                                                                                                                   caccTTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTGGTAGTAACATCATTACCAGCAGTATGATTTCCCGCTATTTCACTCACTCCTTAAATGTAGTGAAAtatt  >  1:166557/1‑251 (MQ=255)                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                       CGCAGCAACGGGTTCGTGGTATTAATGCTGATTATTTAAATCTTTTAAAAAGAGCGTTAAATATCAAATTAACACTCCGGGAATACGCAGATCATCAAAAAGCAATGGACGCGCTTGCAGAAGGTGAAGTCGATATAGTGTTATCACATTTAGTTACTTCGCCGCCTCTTAATAATGACATTGCTGCAACCAAACCATTGATAATTACCTTTCCGGCGCTGGTAACCACCCTTCACGACTCAATGCGACCGCTTACCTCACCAAAACCAGTAAATATTGCTCGGGTAGCAAATTACCCCCCAGACGAGGTAATTCATCAATCATTTCCAAAAGCAACAATTATCTCTTTTACAAATTTATATCAGGCATTAGCATCCGTCTCAGCTGGGCACAATGATTACTTTATTGGTAGTAACATCATTACCAGCAGTATGATTTCCCGCTATTTCACTCACTCCTTAAATGTAGTGAAATATT  >  NC_000913/2484597‑2485073

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑