Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	10,696	C→T	A221T (GCC→ACC)	yaaW ←	putative enzyme‑specific chaperone YaaW

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	10,696	0	C	T	100.0%	23.1 / NA	10	A221T (GCC→ACC)	yaaW	putative enzyme‑specific chaperone YaaW
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (7/3);  total (7/3)

CTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAACTGTAGTGTTTTCAGGGCGCGGCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATCGTCACGCGATAGGCGCTGCCGC‑TGACCGCTTTAACCCCATTTAGTGCCGCACCTACAGGGCCTCCCAGCCCCGCGCCGCGCAGCAAACCATGCCCAAGTACGCTCATTGCTGCGTGGGTGCGTAAAATGCGGGTCAGTTGGCTGGAAAGCAAATGCGACACACCTTTTGCCAATAATTTGTCTTTCATCAGCAGCGGCAGCAGCTCTTCCAGCTCATTCACCCTGGCATCGACCGCG  >  NC_000913/10480‑10940                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                      cgtAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAACTGTAGTGTTTTCAGGGCGCGGCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGTTGGAATCGTCACGCGATAGGCGCTGCCGC‑TGAcc                                                                                                                                                                                                                    <  1:226299/249‑1 (MQ=255)                                               tGAAATAATTTCCCTGAATAACTGTAGTGTTTTCAGGGCGCGGCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGTTGGAATCGTCACGCGATAGGCGCTGCCGC‑TGACCGCTTTAACCCCATTTAGTGCCGCACCTACAGGGCCTCCCAGccccc                                                                                                                                                                      >  2:232402/1‑250 (MQ=255)                                                                                                aatCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGTTGGAATCGTCACGCGATAGGCGCTGCCGC‑TGACCGCTTTAACCCCATTTAGTGCCGCACCTACAGGGCCTCCCAGCCCCGCGCCGCGCAGCAAACCATGCCCAAGTACGCTCATTGCTGCGTGGGTGCg                                                                                                                     >  2:169782/1‑251 (MQ=255)                                                                                                    aGCCAGTGGGGCAGTGTCTACGAGCTTTTGAGGGGAAAAGGAAAATTTTCCCCGGTTTCCGGTAGCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGTTGGAATCGTCACGCGATAGGCGCTGCCGC‑TGACCGCTTTAACCCCATTTAGTGCCGCACCTACAGGGCCTCCCAGCCCCGCGCCGCGCAGCAAACCATGCCCAAGTACGCTCATTGCTGCGTGGGTGCGTaaa                                                                                                                 <  1:26927/251‑1 (MQ=255)                                                                                                                                                                   aTCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGTTGGAATCGTCACGCGATAGGCGCTGCCGC‑TGACCGCTTTAACACCATTTAGTGCCGCACCTACAGGGCCTCCCAGCCCCGCGCCGCGCAGCAAACCATGCCCAAGTACGCTCATTGCTGCGTGGGAGCGTAAAATGCGGGTCAGTTGGCTGGAAAGCAAATGCGACACACCTTTTGCCAATAATTTGGCTTtcatc                                                  >  2:232464/1‑251 (MQ=255)                                                                                                                                                                        aCCTGAGTGGCGCTAACCATCCGGCGCAGGCCGGCGATTTGCAGTACGGTTGGAATCGTGACGCGATAGGCGCTGCCGC‑TGACCGCTTTAACCCCATTTAGTGCCGCACCTACAGGGCCTCCCAGCCCCGCGCCGCGCAGCACACCATGCCCAAGTACGCTCATTGCTGCGTGGGTGCGTAAAATGCGGGTCAGTTGGCTGGAAAGCAAATGCGACACACCTTTTGCCAATAATTTGTCTTTCATcagc                                               >  1:36800/1‑249 (MQ=255)                                                                                                                                                                           tGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGTTGGAATCGTCACGCGATAGGCGCTGCCGC‑TGACCGCTTTAACCCCATTTAGTGCCGCACCTACAGGGCCTCCCAGCCCCGCGCCGCGCAGCAAACCATGCCCAAGTACGCTCATTGCTGCGTGGGTGCGTAAAATGCGGGTCAGTTGGCTGGAAAGCAAATGCGACCCACCTTTTGCCAATAATTTGTCTTTCATCAGCAGCgg                                          >  1:176638/1‑251 (MQ=255)                                                                                                                                                                                                           gATTTGTAGTACGGTTGGAATCGTCACGCTATAGGCGCTGCCGCGTG‑CCTCTTTAACCCCATGTATGGCCGCCCCTACAGGGCCTCCCAGCCCCGCGCCGCGCAGCAAACCATGCCCAAGTACGCTCATTGCTGCGTTGGTGCGTAAAATGCGGGTCAGTTGGCTGGAAATCAAATGCGACACGCCTTTTGCCAATAATTTGTCTTTCAGCAGCAGCGGCAGCAGCTCTTCCAGGTCATTCACCCTGGCAt          <  2:100966/251‑1 (MQ=255)                                                                                                                                                                                                                 cAGTACGGTTGGAATCGTCACGCGATAGGCGCTGCCGC‑TGACCGCTTTAACCCCATTTAGTGCCGCACCTACAGGGCCTCCCAGCCCCGCGCCGCGCAGCAAACCATGCCCAAGTACGCTCATTGCTGCGTGGGTGCGTAAAATGCGGGTCAGTTGGCTGGAAAGCAAATGCGACACACCTTTTGCCAATAATTTGTCTTTCATCAGCAGCGGCAGCAGCTCTTCCAGCTCATTCACCCTGGCATCGACcg    >  1:7021/1‑251 (MQ=255)                                                                                                                                                                                                                   gTACGGTTGGAATCGTCACGCGATAGACGATGCCGC‑TGACCGCTTTAACCCCATTTAGTGCCGCACCTACAGGGCCTCCCAGCCCCGCGCCGCGCAGCAAACCATGCCCACGTACGCTCATTGCTGCGTGGGTGCGTAAAATGCGGGTCAGTTGGCTGGAACGCAAATGCGACACACCTTTTGCCAATAATTTGTCTTTCATCAGCAGCGGCAGCAGCTCTTCCAGCTCATTCACCCTGGCATCGACcgcg  >  2:144675/1‑251 (MQ=255)                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                      CTTAGTGTTGCCCATAATTCCTCAAAAATCATCATCGAATGAATGGTGAAATAATTTCCCTGAATAACTGTAGTGTTTTCAGGGCGCGGCATAATAATCAGCCAGTGGGGCAGTGTCTACGATCTTTTGAGGGGAAAATGAAAATTTTCCCCGGTTTCCGGTATCAGACCTGAGTGGCGCTAACCATCCGGCGCAGGCAGGCGATTTGCAGTACGGCTGGAATCGTCACGCGATAGGCGCTGCCGC‑TGACCGCTTTAACCCCATTTAGTGCCGCACCTACAGGGCCTCCCAGCCCCGCGCCGCGCAGCAAACCATGCCCAAGTACGCTCATTGCTGCGTGGGTGCGTAAAATGCGGGTCAGTTGGCTGGAAAGCAAATGCGACACACCTTTTGCCAATAATTTGTCTTTCATCAGCAGCGGCAGCAGCTCTTCCAGCTCATTCACCCTGGCATCGACCGCG  >  NC_000913/10480‑10940

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑