Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,507,867	G→A	E363K (GAG→AAG)	rlhA →	23S rRNA 5‑hydroxycytidine C2501 synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,507,867	0	G	A	100.0%	103.1 / NA	33	E363K (GAG→AAG)	rlhA	23S rRNA 5‑hydroxycytidine C2501 synthase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (19/14);  total (19/14)

TCCATCGACGGAAAAGACTTTCCACCGTGGTAGCACAGATTATTTTGTGAATGCCCGTAAAGGCGATATTGGCGCGTTCGATTCGCCGAAATTTATCGGCCTGCCGGTAGGCGAAGTAGTGAAAGTGGCGAAAGATCATCTCGATGTTGCCGTTACCGAGCCAC‑TGGCAAATGGCGATGGCCTGAACGTGTTGATTAAACGTGAAGTCGTCGGTTTTCGTGCCAATACGGTCGAGAAAACCGGAGAAAATCAGTACCGCGTCTGGCCCAATGAAATGCCAGCAGATTTGCACAAAATTCGTCCACATCACCCACTAAACCGTAATCTTGATCATAACTGGCAGCAGGCACTGACAAAAACCTCCAGCGAACGTCGGGTGGCGGTAGACATTGAACTGGGCGGCTGGCAGGAACAACTGATTCTGACCCTCACCAGTGAAGAGGGTGTCAGCATCACGCATACGCTGGACGGGCAG  >  NC_000913/1507635‑1508109                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                   tCCATCGACGGAAAAGACTTTCCACCGTGGTAGCACAGATTATTTTGTGAATGCCCGTAAAGGCGATATTGGCGCGTTCAATTCGCCGAAATTTATCGGCCTGCCGGTAGGCGAAGTAGTGAAAGTGGCGAAAGATCATCTCGATGTTGCCGTTACCGAGCCAC‑TGGCAAATGGCGATGGCCTGAACGTGTTGATTAAACGTGAAGTCGTCGGTTTTCGTGCCAATACGGTCAAGAAAACCGGAGAAAAt                                                                                                                                                                                                                                   <  1:190574/250‑1 (MQ=255)      ggaaggAAAAGACTTTCCACCGTGGTAGAACAGATTATTTTGTGAAGGACCGGAAAGGCGATATTGGCGTGTTCGATTCGCCGAAATTTATCGGCCTGCCGGTAGGCGAAGTAGTGAAAGTGGCGAAAGATCATCTCGATGTTGCCGTTACCGAGCCAC‑TGGCAAATGGCGATGGCCTGAACGTGTTGATTAAACGTGAAGTCGTCGGTTTTCGTGCCAATACGGTCAAGAAAACCGGAGAAAATCAGTAc                                                                                                                                                                                                                             <  2:232321/247‑1 (MQ=255)       gACGGAAAAGACTTTCCACCGTGGTAGCACAGATTATTTGGTGAATGCCCGTAAAGGCGATATTGGCGCGTTCGATTCGCCGAAATTTATCGGCCTGCCGGTAGGCGAAGTAGTGAAAGTGGCGAAAGATCATCTCGATGTTGCCGTTACCGAGCCAC‑TGGCAAATGGCGATGGCCTGAACGTGTTGATTAAACGTGAAGTCGTCGGTTTTCGTGCCAATACGGTCAAGAAAACCGGAGAAAATCAGTAcc                                                                                                                                                                                                                            <  1:262175/251‑1 (MQ=255)                    ttCCACCGTGGTAGCACAGATTATTTTGTGAATGCCCGTAAAGGCGATATTGGCGCGTTCGATTCGCCGAAATTTATCGGCCTGCCGGTAGGCGAAGTAGTGAAAGTGGCGAAAGATCATCTCGATGTGGCCGTTACCGAGCCAC‑TGGCAAATGGCGATGGCCTGAACGTGTTGATTAAACGTGAAGTCGTCGGTTTTCGTGCCAATACGGTCAAGAAAACCGGAGAAAATCAGTACCGCGTCTGGCCCa                                                                                                                                                                                                                <  2:66159/250‑1 (MQ=255)                         ccGTGGTAGCACAGATTATTTTGTGAATGCCCGTAAAGGCGATATTGGCGCGTTCGATTCGCCGAAATTTATCGGCCTGCCGGTAGGCGAAGTAGTGAAAGTGGCGAAAGATCATCTCGATGTTGCCGTTACCGAGCCAC‑TGGCAAATGGCGATGGCCTGAACGTGTTGATTAAACGTGAAGTCGTCGGTTTTCGTGCCAATACGGTCAAGAAAACCGGAGAAAATCAGTACCGCGTCTGGCCCAATGaa                                                                                                                                                                                                           >  2:281579/1‑250 (MQ=255)                                aGCACAGATTATTTTGTGAATGCCCGTAAAGGCGATATTGGCGCGTTCGATTCGCCGAAATTTATCGGCCTGCCGGTAGGCGAAGTAGTGAAAGTGGCGAAAGATCATCTCGATGTTGCCGTTACCGAGCCAC‑TGGCAAATGGCGATGGCCTGAACGTGTTGATTAAACGTGAAGTCGTCGGTTTTCGTGCCAATACGGTCAAGAAAACCGGAGAAAATCAGTACCGCGTCTGGCCCAATGAAATGCcag                                                                                                                                                                                                    <  1:120746/250‑1 (MQ=255)                                 gCACAGATTATTTTGTGAATGCCCGGAAAGGCGATATTGGCACGTTCGATTAGCCGAAATTTATCGGCCTGACGGTAGGCGAAGTAGTGACAGTGGCGAAAGATCATCTCGATGTTGCCGTTACCGAGCCAC‑TGGCAAATGGCGATGGCCTGAACGTGTTGATTAAACGTGGAGTCGTCGGTTTTCGCGCCAATAAGGTCAAGAAAAACGGAGAAAATCAGGACCGCGTATGGCCCCATGAAATGCcaacc                                                                                                                                                                                                  >  2:130937/1‑248 (MQ=255)                                                   aTGCCCGTAAAGGCGATATGGGCGCGTTCGATTCGCCAAAATTTATCGGCCTGCCGGTAGGCGAAGTAGTGAAAGTGGCGAAAGATCATCGCGATGTTGCCGTTACCGAGCCAC‑TGGCAAATGGCGATGGCCTGAACGTGTTGATTAAACGTGAAGTCGTCGGTTTTCGTGCCAATACGGTCAAGAAAACCGGAGAAAATCAGTACCGCGTCTGGCCCAATGAAATGCCAGCAGATTTGCACAAAATTCGt                                                                                                                                                                                <  2:250015/251‑1 (MQ=255)                                                                        gcgcGTTCGATTCGCCGAAATTTATCGGCCTGCCGGTAGGCGAAGTAGTGAAAGTGGCGAAAGATCATCTCGATGTTGCCGTTACCGAGCCAC‑TGGCAAATGGCGATGGCCTGAACGTGTTGATTAAACGTGAAGTCGTCGGTTTTCGTGCCAATACGGTCAAGAAAACCGGAGAAAATCAGTACCGCGTCTGGCCCAATGAAATGCCAGCAGATTTGCACAAAATTCGTCCACATCACCCACTAAACCGt                                                                                                                                                           >  1:306817/1‑251 (MQ=255)                                                                                    cGCCGAAATTTACCGGCCTGCCGGTAGGCGAAGTAGTGAAAGTGGCGAAAGATCATCTCGATGTTGCCGTTACCGAGCCAC‑TGGCAAATGGCGATGGCCTGAACGTGTTGATTAAACGTGAAGTCGTCGGTTTTCGTGCCAATACGGTCAAGAAAACCGGAGAAAATCAGTACCGCGTCTGGCCCAATGAAATGCCAGCAGATTTGCACAAAATTCGTCCACATCACCCACTAAACCGTAATCTTGATCa                                                                                                                                                <  2:95571/250‑1 (MQ=255)                                                                                            tttATCGGCCTGCCGGTAGGCGAAGTCGTGAAAGTGGCGAACGATAATCTCGGTGTTGCCGTTACCGAGCCAC‑TGGCAAATGGCGATGGCCTGGACGTGTTGATTAAACCTGAAGTCGTCCGTTTTCGCGCCAATACGATAAAGAAAACCGGGGAACATCTGTCCCGCGCCTGGCCCAATGAAATGCCAGCAGATTTGCACAAAATTCGTCCACATCACCCACTAACCAGTAATCTTGATCCTAACTGgc                                                                                                                                        >  1:139091/1‑250 (MQ=255)                                                                                                            tAGGCGAAGTAGTGAAAGTGGCGAAAGATCATCTCGATGTTGCCGTTACCGAGCCAC‑TGGCAAATGGCGATGGCCTGAACGTGTTGATTAAACGTGAAGTCGTCGGTTTTCGTGCCAATACGGTCAAGAAAACCGGAGAAAATCAGTACCGCGTCTGGCCCAATGAAATGCCAGCAGATTTGc                                                                                                                                                                                           >  2:126147/1‑183 (MQ=255)                                                                                                            tAGGCGAAGTAGTGAAAGTGGCGAAAGATCATCTCGATGTTGCCGTTACCGAGCCAC‑TGGCAAATGGCGATGGCCTGAACGTGTTGATTAAACGTGAAGTCGTCGGTTTTCGTGCCAATACGGTCAAGAAAACCGGAGAAAATCAGTACCGCGTCTGGCCCAATGAAATGCCAGCAGATTTGc                                                                                                                                                                                           <  1:126147/183‑1 (MQ=255)                                                                                                                                gCGAAAGATCATCTCGATGTGGCCGTTACCGAGCCAC‑TGGCAAATGGCGATGGCCTGAACGTGTTGATTAAACGTGAAGTCGTCGGTTTTCGTGCCAATACGGTCAAGAAAACCGGAGAAAATCAGTACCGCGTCTGGCCCAATGAAATGCCAGCAGATTTGCACAAAATTCGTCCACATCACCCACTAAACCGTAATCTTGATCATAACTGGCAGCAGGCACTGACAAAAACCTCCAGCGAACGTCGGGt                                                                                                   <  1:281579/251‑1 (MQ=255)                                                                                                                                  gAAAGATCATCTCGATGTTGCCGTTACCGAGCCAC‑TGGCAAATGGCGATGGCCTGAACGTGTTGATTAAACGTGAAGTCGTCGGTTTTCGTGCCAATACGGTCAAGAAAACCGGAGAAAATCAGTACCGCGTCTGGCCCAATGAAATGCCAGCAGATTTGCACAAAATTCGTCCACATCACCCACTAAACCGTAATCTTGATCATAACTGGCAGCAGGCACTGACAAAAACCTCCAGCGAACGTCGGGTgg                                                                                                 >  1:257708/1‑251 (MQ=255)                                                                                                                                        tcatcTCGATGTTGCCGTTCCCGAGCCAC‑TGGCAAATGGCGATGGCCTGAACGTGTTGATTAAACGTGAAGTCGTCGGTTTTCGTGCCAATACGGTCAATAAAACCGGAGAAAATCAGTACCGCGTCTGGCCCAATGAAATGCCAGCAGATTTGCACAAAATTCGTCCACATCACCCACTAAACCGTAATCTTGATCATAACTGGCAGCAGGCACTGACAAAAACCTCCAGCGAACGTCGGGTGGCGGTa                                                                                            <  1:130937/250‑1 (MQ=255)                                                                                                                                                aTGTTGCCGTTACCGAGCCAC‑TGGCAAATGGCGATGGCCTGAACGTGTTGATTAAACGTGAAGTCGTCGGTTTTCGTGCCAATACGGTCAAGAAAACCGGAGAAAATCAGTACCGCGTCTGGCCCAATGAAATGCCAGCAGATTTGCACAAAATTCGTCCACATCACCCACTAAACCGTAATCTTGATCATAACTGGCAGCAGGCACTGACAAAAACCTCCAGCGAACGTCGGGTGGCGGTAGACATTGa                                                                                    >  1:135706/1‑250 (MQ=255)                                                                                                                                                 tGTTGACGTTACCGAGCCAC‑TGGCAAATGGCGATGGCCTGAACGTGTTGATTAAACGTGAAGTCGTCGGTTTTCGTGCCCATACGGTCAAGAAAACCGGAGAAAATCAGTACCGCGTCTGGCCCAATGAAATGCCAGCAGATTTGCACAAAATTCGTCCACATCACCCACTAAACCGTAATCTTGATCATAACTGGCAGCAGGCACTGACAAAAACCTCCAGCGAACGTCGGGTGGCGGTAGACATTGAAc                                                                                  >  2:180806/1‑251 (MQ=255)                                                                                                                                                       cGTTACCGAGC‑ACATGGCAAATGGCGATGGCTTGAACGTGTTGATTACACGAGAAGACGCCGGTTTTCGTGCAAATAAGGTCAAGAAAACCGGAGAAAATCAGTACCGCGTCTGGCGCAATGAAATGCCAGCAGATTTGCACAAAATACGTCCACATCACCCACTAAACCGTAATCTTGATCATAACTGGCAGCAGGCACTGACAATAACCTCCAGCGAACGTAGGGAGGCGGAAGACATTGAACTggacg                                                                            >  2:185416/1‑248 (MQ=255)                                                                                                                                                        gTTACCGAGCCAC‑TGGCAAATGGCGATGGCCTGAACGTGTTGATTAAACGTGAAGTCGTCGGTTTTCGTGCCAATACGGTCAAGAAAACCGGAGAAAATCAGTACCGCGTCTGGCCCAATGAAATGCCAGCAGATTTGCACAAAATTCGTCCACATCACCCACTAAACCGTAATCTTGATCATAACTGGCAGCAGGAACTGACAAAAACCTCCAGCGACCGTCGGGTGGCGGTAGACATTGAACTGggcgg                                                                           >  1:174430/1‑251 (MQ=255)                                                                                                                                                                 ccAC‑TGGCTAATGGCGATGGCCTGAACGTGTTGATTAAACGTGTAGTCGTCGGTTTTCGTGCCAATAAGGTCAAGAAAACCGGAGCAAATCAGTACCGCGTCTGGCCCAATGAAATGCCAGCATGTTTGCACAAACTTCGTCCACATCACCCCCTAACCCGTAATCTTGATCATAACTGGCAGCAGGACCTGCCAAAAACCTCATGCGACCGTCGGGTGGCGGTAGACATTGGACTAGCCGGCTGGCAggc                                                                  >  1:301200/1‑250 (MQ=255)                                                                                                                                                                  cAC‑TGGCAAATGGCGATGGCCTGAACGTGTGGATTAAACGTGAAGTCGTCGGTTTTCGTGCCAATACGGTCAAGAAAACCGGAGAAAATCAGTACCGCGTCTGGCACAATGAAATGCCAGCAGATTTGCACAAAATTCGTCCACATCACCCACTAAACCGTAATCTTGATCATAACTGGCAGCAGGCACTGACAAAAACCTCCAGCGAACGTCGGGTGGCGGTAGACATTGAACGGGGCGGCTGGCAGGaa                                                                 <  2:253909/251‑1 (MQ=255)                                                                                                                                                                       ggCAAATGGCGATGGCCTGAACGTGTTGATTAAACGTGAAGTCGTCGGTTTTCGTGCCAATACGGTCAAGAAAACCGGAGAAAATCAGTACCGCGTCTGGCCCAATGAAATGCCAGCAGATTTGCACAAAATTCGTCCACATCACCCACTAAACCGTAATCTTGATCATAACTGGCAGCAGGCACTGACAAAAACCTCCAGCGAACGTCGGGTGGCGGTAGCCATTGAACTGGGCGGCTGGCAGGaacaac                                                             >  2:85828/1‑251 (MQ=255)                                                                                                                                                                       ggCAAATGGCGATGGCCTGAACGTGTTGATTAAACGCGAATGCGTCGGTGTTCGTGCCAATACGGTCAAGAAAACCGGAGAAAATCAGTACCGCGTCTGGCCCAATGAAATGCCAGCAGATTTGCACAAAATTCGTCCACATCACCCACTAAACCGTAATCTTGAGCATAACTGGCAGCATGCACGGACAAAAACCTCCAGCGAACGTCGAGTGGCGGTAGACAGTTAACTGTGCGGCTGGCAGTaacaa                                                              <  2:155800/250‑1 (MQ=255)                                                                                                                                                                                    ggCCTGAACGTGTTGATTAAACGTGAAGTAGTCGGTTTTCGTGCCAATACGGTCAAGAAAACCGGAGAAAATCAGTACCGCGTCTGGCCCAATGAAATGCCAGCAGATTTGCACAAAATTCGTCCACATCACCCACTAAACCGTAATCTTGATCATAACTGGCAGCAGGCACTGACAAAAACCTCCAGCGAACGTCGGGTGGCGGTAGACATTGAACTGGGCGGCTGGCAGGAACAACTGATTCTGAccc                                                 <  1:169910/250‑1 (MQ=255)                                                                                                                                                                                                   aTTAAACGTGAAGTCGTCGGTTTTCGTGCCAATACGGTCAAGAAAACCGGAGAAAGTCAGTACCGCGTCTGGCCCAATGAAATGCCAGCAGATTTGCACAAAATTCGTCCACATCACCCACTAAACCGTAATCTTGATCATAACTGGCAGCAGGCACTGACAAAAACCTCCAGCGAACGTCGGGTGGCGGTAGACATTGAACTGGGCGGCTGGCAGGAACAACTGATTCTGACCCTCACCAGTGAAGAggg                                 >  1:23604/1‑251 (MQ=255)                                                                                                                                                                                                   aTTAAACGTGAAGTCGTCGGTTTTCGTGCCAATACGGTCAAGAAAACCGGAGAAAATCAGTACCGCGTCTGGCCCAATGAAATGCCAGCAGATTTGCACAAAATTCGTCCACATCACCCACTAAACCGTAATCTTGATCATAACTGGCAGCAGGCACTGACAAAAACCTCCAGCGAACGTCGGGTGGCGGTAGACATTGAACTGGGCGGCTGGCAGGAACAACTGATTCTGCCCCTCACCAGTGAAGAggg                                 >  2:192403/1‑251 (MQ=255)                                                                                                                                                                                                       aaCGTGAAGTCGTCGGATTTCGTGCCAATACGGTCAAGAAAACCGGAGAAAATCAGTACCGCGTCTGGCCCAATGAAATGCCAGCAGATTTGCACAAAATTCGTCCACATCACCCACTAAACCGTAATCTTGATCATAACTGGCAGCAGGCACTGACAAAAACCTCCAGCGAACGTCGGGTGGCGGTAGACATTGAACTGGGCGGCTGGCAGGAACAACTGATTCTGACCCTCACCAGTGAAGAGGGTGTc                             >  1:64508/1‑251 (MQ=255)                                                                                                                                                                                                                     ggTTTTCGTGCCAATACGGTCAAGAAAACCGGAGAAAATCAGTACCGCGTCTGGCCCAATGAAATGCCAGCAGATTTGCACAAAATTCGTCCACATCACCCACTAAACCGTAATCTTGATCATAACTGGCAGCAGGCACTGACAAAAACCTCCAGCGAACGTCGGGTGGCGGTAGACATTGAACTGGGCGGCTGGCAGGAACAACTGATTCTGACCCTCACCAGTGAAGAGGGTGTCAGCATCACGCATAc               >  1:21644/1‑251 (MQ=255)                                                                                                                                                                                                                     ggTTTTCGAGCCAATACGGTCACGAAAACCGGAGAAAGTCAGCACCGCGTATGGCCCAATGAAAAGCCAGCAGATTTGCACAAAATTCGTCCACATCAACCACTAAACCGTAATCTTGATCATAACTAGCAGCAGGCACTGACAAAAAACTCCAGAGATCGTCGGGTGGCGGTAGGCATTGAACTGGGAGGCTGGCAGGAACAACTGAATCTGACCCTCACCAGTGAAGAGGGAGTCAGCATCACGCATAc               >  2:277791/1‑251 (MQ=255)                                                                                                                                                                                                                     ggTTTACGTGCCACTACGGTCACGAAAAACGGAGAAACTAAGTACCGCGTCTGGCCAAATGAAATGCCAGCAGATTTGAACAAAATTCGTACACATCAACCACTAAACCGTAATCTTGATCATAACTGGCAGCAGGCCCTGACAAACACCTCCAGCGAACGCAGGGTGGCGGTCGACCTTGCACTGGGCGGCTGGCAGGAACAACTGCTTCTGCACCTCACCAGTGAAGAGGGTGTCAGCATCACGCATAc               >  2:137739/1‑251 (MQ=255)                                                                                                                                                                                                                           cGTGCAAATACGGTCAAGAAAACCGGAGAAAATCAGTACCGCGTCTGGCCCAATGAAATGCCAGCAGATTTGCACAAAATTCGTCCACATCACCCACTAAACCGTAATCTTGATCATAACTGGCAGCAGGCACTGACAAAAACCTCCAGCGAACGTCGGGTGGCGGTAGACATTGAACTGGGCGGCTGGCAGGAACAACTGATTCTGACCCTAACCAGTGAAGAGGGGGTCAGCATCACgactaagctgg          >  2:215024/1‑240 (MQ=255)                                                                                                                                                                                                                                  aTACGGTCAAGAAAACCGGAGAAAAGCAGTACCGCGGCTGGCCCAATGAAATGCCAGCAGAGTTGCACAAAATTCGGCCACAGCACCCACTAAACCGTAAGCGTGATCATAACTGGCAGCAGGCACTGACAAAAACCTCCAGCGAACGTCGGGGGGCGGTAGACATGGAGCTGGGCTGCTGGCAGGAAGAACGGATGCGGACCCTCACCAGTGAAGAGGGTGTCATCATCACGCATACGCTGGACGGGCAg  <  2:139091/251‑1 (MQ=255)                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                   TCCATCGACGGAAAAGACTTTCCACCGTGGTAGCACAGATTATTTTGTGAATGCCCGTAAAGGCGATATTGGCGCGTTCGATTCGCCGAAATTTATCGGCCTGCCGGTAGGCGAAGTAGTGAAAGTGGCGAAAGATCATCTCGATGTTGCCGTTACCGAGCCAC‑TGGCAAATGGCGATGGCCTGAACGTGTTGATTAAACGTGAAGTCGTCGGTTTTCGTGCCAATACGGTCGAGAAAACCGGAGAAAATCAGTACCGCGTCTGGCCCAATGAAATGCCAGCAGATTTGCACAAAATTCGTCCACATCACCCACTAAACCGTAATCTTGATCATAACTGGCAGCAGGCACTGACAAAAACCTCCAGCGAACGTCGGGTGGCGGTAGACATTGAACTGGGCGGCTGGCAGGAACAACTGATTCTGACCCTCACCAGTGAAGAGGGTGTCAGCATCACGCATACGCTGGACGGGCAG  >  NC_000913/1507635‑1508109

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑