Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,301,998	A→G	S273G (AGC→GGC)	oppA →	oligopeptide ABC transporter periplasmic binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,301,998	0	A	G	100.0%	43.1 / NA	14	S273G (AGC→GGC)	oppA	oligopeptide ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (9/5);  total (9/5)

AATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTATGGTTACACTCCACCGTATACTGATGGCGCAAAATTG  >  NC_000913/1301780‑1302240                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                   aaTTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACATTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACGGCATGCCGATCGAATTGTTCCAGAAGCTGaa                                                                                                                                                                                                                     >  2:270890/1‑250 (MQ=255)        cGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACGGCATGCCGATCGAATTGGTCTAGAAGCTGAAAAAAgaga                                                                                                                                                                                                             <  2:59985/251‑1 (MQ=255)                              aaTATCATCACCATGGGTGCCTATACCTTAAAAGATTGTGACGTAAACTAACGAATCTTTGTTGAACGCAGCCCGACCTACTGGAACAACGCTGAAACCGTTATTAACCATGTAACCTATTTGCCTATTGCTTGTGATGTTACCGATGACAACCGCTACCGTAGGGTTGAAATGGAGATGACGAAGAACGGCAGGCCTATCGAATGGTTCCAGAAGCGGAGAAAAGAGGTccttcacgaaattcgcgttg                                                                                                                                                                                        <  2:300916/250‑19 (MQ=255)                                               tGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACGGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACtt                                                                                                                                                                      >  2:91472/1‑251 (MQ=255)                                                           aaaaGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACGGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGaaa                                                                                                                                                          <  1:53091/251‑1 (MQ=255)                                                                                                        gACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACGGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCg                                                                                                             >  1:194960/1‑251 (MQ=255)                                                                                                                      atgcgAAAACCGTTATAAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGATACCGTAGTGGTGAAATCGACATGACGAATAACGGCATGCCGATCGAATTGTGCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGACCCTTCCCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGt                                                                                               <  2:61718/249‑1 (MQ=255)                                                                                                                      acGCGAAAACCGATATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTCCCGATGTCAACCGCTCCCGTAGTGGTGAAATCGACCTGACGAATAACGGCATGCCGATCGAATTGTTACAGAAGATGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCCGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGt                                                                                               >  2:18011/1‑251 (MQ=255)                                                                                                                       cgcgAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACTTGACGAATAACGGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATAACTGTGCACTTACTATTACGCAATTAACAACCAGAAACCGCCCATCAACGATGTGCGTGTGCGTACCGCCCTGCAACTAGGt                                                                                               >  2:182415/1‑250 (MQ=255)                                                                                                                                  ttattaACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACGGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGat                                                                                   >  1:27098/1‑251 (MQ=255)                                                                                                                                                                      aaGTTACAGCTGTCAACCGCTACCCTAGTGGTCAAATCGACATGAAGAATAACGGCATGCCGATCGAATTGCTCCAGAAGCCGAAAAAAGAGATCCCGGAAGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACACGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAAcctg                                               >  2:16683/1‑248 (MQ=255)                                                                                                                                                                                                       aaaTCGACATGATGAAAAACGGATTGCCGATCAAATTGTCCCAGAAGCTGAAAAAAGATATCCCGGAGGAAGTCCATGTTGATCCATCCCTGTGCACTAACTATTACGAAATTAACACCCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAACCTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTATGGTTACACTCCACCGTATACTGAt              <  1:182415/251‑1 (MQ=255)                                                                                                                                                                                                             aCATGACGAATAACGGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTATGGTTACACTCCACCGTATACTGATGgcgc         >  1:280727/1‑250 (MQ=255)                                                                                                                                                                                                                    gAATAACGGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTATGGTTACACTCCACCGTATACTGATGGCGCAAAATTg  >  1:150116/1‑250 (MQ=255)                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                   AATTCGGCGAAAAATGGACCCAGCCTGGTAATATCGTCACCAACGGTGCCTATACCTTAAAAGATTGGGTCGTAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAAATCGACATGACGAATAACAGCATGCCGATCGAATTGTTCCAGAAGCTGAAAAAAGAGATCCCGGACGAAGTTCACGTTGATCCATACCTGTGCACTTACTATTACGAAATTAACAACCAGAAACCGCCATTCAACGATGTGCGTGTGCGTACCGCACTGAAACTAGGTATGGACCGCGATATCATTGTTAATAAAGTGAAAGCGCAGGGCAACATGCCCGCCTATGGTTACACTCCACCGTATACTGATGGCGCAAAATTG  >  NC_000913/1301780‑1302240

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑