Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,903	G→A	E523K (GAA→AAA)	thrA →	fused aspartate kinase/homoserine dehydrogenase 1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,903	0	G	A	100.0%	73.8 / NA	24	E523K (GAA→AAA)	thrA	fused aspartate kinase/homoserine dehydrogenase 1
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (15/9);  total (15/9)

GTGGTAAATAACGATGATGCGACCACTGGCGTGCGCGTTACTCATCAGATGCTGTTCAATACCGATCAGGTTATCGAAGTGTTTGTGATTGGCGTCGGTGGCGTTGGCGGTGCGCTGCTGGAGCAACTGAAGCGTCAGCAAAGCTGGCTGAAGAATAAACATATCGACTTACGTGTCTGCGGTGTTGCCAACTCGAAGGCTCTGCTCACCAAT‑GTACATGGCCTTAATCTGGAAAACTGGCAGGAAGAACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATT  >  NC_000913/1660‑2098                                                                                                                                                                                                                                                     |                                                                                                                                                                                                    gTGGTAAATAACGATGATGCGACCACTGGCGTGCGCGTTACTCATCAGATGCTGTTCAATACCGATCAGGTTATCGAAGTGTTTATGACTCGCCTCGGTGACGTTCGCGATGCGCTCCTGGAGCAACTGAAGCGTCAGCACAGCTGGCTGAAGACTAAACATATCGTCTTACGTGTCTGCGGAGTTGCCCACTCGATGGCACTGCTCACCAAT‑GCACATGGCCTTAATCTGGAAAAGTGGCAGAAAGAACt                                                                                                                                                                                              >  1:141176/1‑251 (MQ=255)                gagtcGACCACTGGCGTGCGCTTTACTCAGCAGATGCTGTGCAATACCGATCAGGTTATCGAAGTGTGTGTGATTGGCGTCGGTGGCGTTGGCGGTGCGCTGCTGGAGCAACTGAAGCGTCAGCAAAGCTGGCTGAAGAATAAACATATCGACTTACGTGTCTGCTGTGTTGCCAACTCGAAGGCGCTGCTCACCAAT‑GTACATGGACTGTATCTGGAAAACTGGCAGAAAGAACTGGCGCAAGCCAAaga                                                                                                                                                                               <  2:158728/247‑1 (MQ=255)                       ccACTGGCGTGCGCGTTACTCATCAGATGCTGTTCAATACCGATCAGGTTATCGAAGTGTTTGTGATTGGCGTCGGTGGCGTTGGCGGTGCGCTGCTGGAGCAACTGAAGCGTCAGCAAAGCTGGCTGAAGAATAAACATATCGACTTACGTGTCTGCGGTGTTGCCAACTCGAAGGCTCTGCTCACCAAT‑GTACATGGCCTTAATCTGGAAAACTGGCAGAAAGAACTGGCGCAAGCCAAAGAGCCGttt                                                                                                                                                                        <  1:267896/251‑1 (MQ=255)                            ggCGTGCGCGTTACTCATCAGATGCTGTTCAATGCCGATCAGGTTATCGAAGTGTTTGTGATTGGCGTCGGTGGCGTTGGCGGTGCGCTGCTGGAGCAACTGAAGCGTCAGCAAAGCTGGCTGAAGAATAAACATATCGACTTACGTGTCTGCGGTGTTGCCAACTCGAAGGCTCGGCTCACCAAT‑GTACATGGCCTTAATCTGTAAAACTGGCAGAAAGAACGGGCGCAAGCCAAAGAGCCGTTTaaa                                                                                                                                                                     <  2:107691/249‑2 (MQ=255)                                 gcgcGTTACTCATCAGATGCTGTTCAATACCGATCAGGTTATCGAAGTGTTTGTGATTGGCGTCGGTGGCGTTGGCGGTGCGCTGCTGGAGCAACTGAAGCGTCAGCAAAGCTGGCTGAAGAATAAACATATCGACTTACGTGTCTGCGGTGTTGCCAACTCGAAGGCTCTGCTCACCAAT‑GTACATGGCCTTAATCTGGAAAACTGGCAGAAAGAACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGgc                                                                                                                                                              <  2:145307/251‑1 (MQ=255)                                                        tCAATACCGATCAGGTTATCGAAGTGTTTGTGATTGGCGTCGGTGGCGTTGGCGGTGCGCTGCTGGAGCAACTGAAGCGTCAGCAAAGCTGGCTGAAGAATAAACATATCGACTTACGTGTCTGCGGTGTTGCCAACTCGAAGGCTCTGCTCACCAAT‑GTACATGGCCTTAATCTGGAAAACTGGCAGAAAGAACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTAGCCTCGTGAAAGa                                                                                                                                        <  2:60436/250‑1 (MQ=255)                                                          aaTACCGATCAGGTTATCGAAGTGTTTGTGATTGGCGTCGGTGGCGTTGGCGGTGCGCTGCTGGAGCAACTGAAGCGTCAGCAAAGCTGGCTGAAGAATAAACATATCGACTTACGTGTCTGCGGTGTTGCCAACTCGAAGGCTCTGCTCACCAAT‑GTACATGGCCTTAATCTGGAAAACTGGCAGAAAGAACTGGCGCACGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAat                                                                                                                                      >  1:65678/1‑250 (MQ=255)                                                             aCCGATCAGGTTATCGAAGTGTTTGTGATTGGCGTCGGTGGCGTTGGCGGTGCGCTGCTGGAGCAACTGAAGCGTCAGCAAAGCTGGCTGAAGAATAAACATATCGACTTACGTGTCTGCGGTGTTGCCAACTCGAAGGCTCTGCTCACCAAG‑GTACATGGCCTTAATCTGGAAAACTGGCAGAAAGAACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATatc                                                                                                                                   <  1:107999/250‑1 (MQ=255)                                                              ccGATCAGGTTATCGAAGTGTTTGTGATTGGCGTCGGTGGCGTTGGCGGTGAGCTGCTGGAGCAACTGAAGCGTCAGAAAAGCTGGCTGAAGAATAAACATATCGACTTACGTGTCTGCGGTGTTGCCAACTCGAAGGCTCTGCTCACCAAT‑GTACATGGCCTTAATCTGGAAAACTGGCAGAAAGAACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGCAAGAATatcat                                                                                                                                 >  2:185045/1‑251 (MQ=255)                                                                   cAGGTTATCGAAGTGTTTGTGATTGGCGTCGGTGGCGTTGGCGGTGCGCTGCTGGAGCAACTGAAGCGTCAGCAAAGCTGGCTGAAGAATAAACATATCGACTTACGTGTCTGCGGTGTTGCCAACTCGAAGGCTCTGCTCACCAAT‑GTACATGGCCTTAATCTGGAAAACTGGCAGAAAGAACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATctgct                                                                                                                            >  1:139500/1‑251 (MQ=255)                                                                         aTCGAAGTGTTTGTGATTGGCGTCGGTGGCGTTGGCGGTGAGCTGCTGGAGCAACTGAAGCGTCAGCAAAGCTGGCTGAAGAATAAACATATCGACTTACGTGTCTGCGGTGTTGCCAACTCGAAGGCTCTGCTCACCAAT‑GTACATGGCCTTAATCTGGAAAACTGGCAGAAAGCACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAAccc                                                                                                                      >  2:140646/1‑251 (MQ=255)                                                                                                 ggTGGCGTTGGCGGTGCGCTGCTGGAGCAACTGAAGCGTCAGCAAAGCTGGCTGAAGAATAAACATATCGACTTACGTGTCTGCGGTGTTGCCAACTCGAAGGCTCTGCTCACCAAT‑GTACATGGCCTTAATCTGGAAAACTGGCAGAAAGAACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTcc                                                                                                >  2:75834/1‑249 (MQ=255)                                                                                                                             aaCTGAAGCGTCAGCAAAGCTGGCTGAAGAATAAACATATCGACTTACGTGTCTGCGGTGTTGCCAACTCGACGGCTCTGCTCACCAAT‑GTACATGGCCTTAATCTGGAAAACTGGCAGAAAGAACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGCTCAATATGCCGAc                                                                  >  2:59750/1‑251 (MQ=255)                                                                                                                              aCTGAAGCGTCAGCAAAGCTGGCTGAAGAATAAACATATCGACTTACGTGTCTGCGGTGTTGCCAACTCGAAGGCTCTGCTCACCAAT‑GTACATGGCCTTAATCTGGAAAACTGGCAGAAAGAACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTg                                                                                    >  1:248391/1‑232 (MQ=255)                                                                                                                              aCTGAAGCGTCAGCAAAGCTGGCTGAAGAATAAACATATCGACTTACGTGTCTGCGGTGTTGCCAACTCGAAGGCTCTGCTCACCAAT‑GTACATGGCCTTAATCTGGAAAACTGGCAGAAAGAACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTg                                                                                    <  2:248391/232‑1 (MQ=255)                                                                                                                                      gTCAGCAAAGCTGGCTGAAGAATAAACATATCGACTTACGTGTCTGCGGTGTTGCCAACTCGAAGGCTCTGCTCACCAAT‑GTACATGGCCTTAATCTGGAAAACTGGCAGAAAGAACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTgcgc                                                         >  1:132552/1‑251 (MQ=255)                                                                                                                                               gctggctgAAGAATAAACATATCGACTTACGTGTATGCGGTGTTGCCAACTCGAAGGCTCTGCTCACCAAT‑GTACATGGCCTTAATCTGGAAAACTGGCAGAAAGAACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGATTc                                                >  1:234762/1‑251 (MQ=255)                                                                                                                                                    ctgAAGAATAAACATATCGACTTACGTGTCTGCGGTGTTGCCAACTCGAAGGCTCTGCTCACCAAT‑GTACATGGCCTTAATCTGGAAAACTGGCAGAAAGAACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCACGt                                           >  1:115479/1‑251 (MQ=255)                                                                                                                                                     tgAAGAATAAACATATCGACTTACGTGTCTGCGGTGTTGCCAACTCGAAGGCTCTGCTCACCAAT‑GTACATGGCCTTAATCTGGAAAACTGGCAGAAAGAACTGGCGCAAGCCAAAGAGCCGGTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTTCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCACGt                                           <  1:182399/250‑1 (MQ=255)                                                                                                                                                         gaaTAAACATATCGACTTACGTGTCTGCGGTGTTGCAAACTCGAAGGCTCTGCTCACCAATTGT‑CATGGCCGTAAACTGGAAAACTGGCAGAAAGAACTGGCGCAAGCCAAAGATCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGAGCAATATGCCGACTTCCTGCGCGAAGGTTTCCACGTTGTCa                                      <  2:210422/251‑1 (MQ=255)                                                                                                                                                                  tatCGACTTACGTGTCTGCGGTGTTGCCAACTCGAAGGCTCTGCTCACCAAT‑GTACATGGCCTTAATCTGGAAAACTGGCAGAAAGAACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCACGTTGTCACGCCGAAc                              >  1:202274/1‑250 (MQ=255)                                                                                                                                                                     cGACTTACGTGTCTGCGGTGTTGCCAACTCGAAGGCTCTGCTCACCAAT‑GTACATGGCCTTAATCTGGAAAACTGGCAGAAAGAACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGTTACATATGCCGACTTCCTGCGCGAAGGGTTccccggtggtaccccgaacaac                           >  2:6320/1‑230 (MQ=255)                                                                                                                                                                                       tgtTGCCAACTCGAAGGCTCTGCTCACCAAT‑GTACATGGCCTTAATCTGGAAAACTGGCAGAAAGAACTGGCGCCAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGAATGCACTTACAGCCCGGCAGTGGCTGATAAATATGGCGCATTACTGCGCGAAGATTTCCACGTTGTCACCCCGAACAAAAAGGCCCACACCtcgtcg         >  1:272869/1‑250 (MQ=255)                                                                                                                                                                                              aaCTCGAAGGCTCTGCTCACCAAT‑GTACATGGCCTTAATCTGGAAAACTGGCAGAAAGAACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGCTggctt  >  1:284474/1‑247 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                    GTGGTAAATAACGATGATGCGACCACTGGCGTGCGCGTTACTCATCAGATGCTGTTCAATACCGATCAGGTTATCGAAGTGTTTGTGATTGGCGTCGGTGGCGTTGGCGGTGCGCTGCTGGAGCAACTGAAGCGTCAGCAAAGCTGGCTGAAGAATAAACATATCGACTTACGTGTCTGCGGTGTTGCCAACTCGAAGGCTCTGCTCACCAAT‑GTACATGGCCTTAATCTGGAAAACTGGCAGGAAGAACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTGGCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATT  >  NC_000913/1660‑2098

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑