Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,778,5690CA32.6% 49.4 / 30.9 43P391T (CCG→ACG) lldPlactate/glycolate:H(+) symporter LldP
Reads supporting (aligned to +/- strand):  ref base C (18/11);  new base A (6/8);  total (24/19)
Fisher's exact test for biased strand distribution p-value = 3.29e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.14e-01
Rejected as consensus: Frequency below/above cutoff threshold.

GTTCCTGACAGCTACCGTAACACTGTGGAGTATCCCGCCGTTTAAA‑GCCCTGTTCGCATCGGGTGGCGCGCTGTATGAGTGGGTGATCAATATTCCGGTGCCGTACCTCGATAAACTGGTT‑GCCCGTATGCCGCCAGTGGTCAGCGAGGCTACAGCCTATGCCGCCGTGTTTAAGTTTGACTGGT‑TCT‑CTGCCACCGGCACCGCCATTCTGTTTGCTGCACTGCTCTCGATTGTCTGGCTGAAGATGAAACCGTCTGACGCTATCAGCACCTTCGGCAGCACGCTGAAAGAACTGGCTCTGCCCATCTACTCCATCGGTATGGTGCTGGCATTCGCCTTTATTTCGAACTATTCCGGACTGTCATCAACACTGGCGCTGGCACTGGCGCACACCGGTCATGCATTCACCTTCTTCTCGCCGTTCCTCGGCTGGCTGGGGGTATTCCTGACCGGGTCGGATACCTCATCTAACGCCCTGTTCGCCGCGCTGCAAGCCAC  >  NC_000913/3778319‑3778816
                                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                       
gttcctgACAGCTACCGTAACACTGTGGAGTATCCCGCCGTTTAAA‑GCCCTGTTCGCATCGGGTGGCGCGCTGTATGAGTGGGTGATCAATATTCCGGTGCCGTACCTCGATAAACTGGTT‑GCCCGTATGCCGCCAGTGGTCAGCGAGGCTACAGCCTATGCCGCCGTGTTTAAGTTTGACTGGT‑TCT‑CTGCCACCGGCACCGCCATTCTGTTTGCTGCACTGCTCTCGATTGTCTGGCTGAAGATGAAAc                                                                                                                                                                                                                                                         >  2:234238/1‑251 (MQ=255)
            tACCGTAACACTGTGGAGTATCCCGCCGTTTAAA‑GCCCTGTTCGCATCGGGTGGCGCGCTGTATGAGTGGGTGATCAATATTCCGGTGCCGTACCTCGATAAACTGGTT‑GCCCGTATGCCGCCAGTGGTCAGCGAGGCTACAGCCTATGCCGCCGTGTTTAAGTTTGACTGGT‑TCT‑CTGCCACCGGCACCGCCATTCTGTTTGCTGCACTGCTCTCGATTGTCTGGCTGCAGATGAAAACGTCTGACGCt                                                                                                                                                                                                                                              >  2:136937/1‑250 (MQ=255)
                  aaCACTGTGGAGTATCCCGCCGTTTAAA‑GCCCTGTTCGCATCGGGTGGCGCGCTGTATGAGTGGGTGATCAATATTCCGGTGCCGTACCTCGATAAACTGGTT‑GCCCGTATGCCGCCAGTGGTCAGCGAGGCTACAGCCTATGCCGCCGTGTTTAAGTTTGACTGGT‑TCT‑CTGCCACCGGCACCGCCATTCTGTTTGCTGCACTGCTCTCGATTGTCTGGCTGAAGATGAAACCGTCTGACGCTATCAGc                                                                                                                                                                                                                                        >  1:1481/1‑250 (MQ=255)
                    ccgtgggggttacccccgcgTTTAAAAGCCCTTTTCGCACCGGGGGGCGCGCTGTAGGGGGGGGTGATAATTATTCCGGTGCGTTCCCCCGA‑AAACCGGTTTGCCCGTATGCCGCCGGTGGTCGGCGAGGCTACAGCCTTGGCCGCCTTGTTTAAGTTTGACTGGT‑TCT‑CTGCCCCCGGAACCGCCATTCTGTTTGCTGCACTGCTCTCGATTGTCTGGCTGAAGATGAAACCGTCTGACGCTATCAGCAc                                                                                                                                                                                                                                      <  2:229993/233‑1 (MQ=255)
                             gTATCCCGCCGTTTAAA‑GCCCTGTTCGCATCGGGTGGCGCGCTGTATGAGTGGGTGATCAATATTCCGGTGCCGTACCTCGATAAACTGGTT‑GCCCGTATGCCGCCAGTGGTCAGCGAGGCTACAGCCTATGCCGCCGTGTTTAAGTTTGACTGGT‑TCT‑CTGCCACCGGCACCGCCATTCTGTTTGCTGCACTGCTCTCGATTGTCTGGCTGAAGATGAAACCGTCTGACGCTATCAGCACCTTCGgcagc                                                                                                                                                                                                                            >  1:47661/1‑251 (MQ=255)
                               atcccccagattAAA‑GCACTGTTCGAATCGGGAGGCGCGATGTCTGAGAGGGTGATCAATATTCCGGTGCCGTACCTCGCTAAACTGGTT‑GCCCGTATGCCGCCAGTGGTCAGCGAGGCTACAGGCTATGCCGCCGTGTTTCAGTTTGCATGGT‑TCT‑CTGCCACCGGCACCGCCATTCTGTTTGCTGCACTGCTCTCGATTGTCTGGCTGAAGATGACACCGTCTGACGCTCTCAGCACCTTCGGCAGCAc                                                                                                                                                                                                                          >  2:150230/11‑251 (MQ=255)
                                    gccgTTTAAA‑GCCCTGTTCGCATCGGGTGGCGCGCTGTATGAGTGGGTGATCAATATTCCGGTACCGTACCTCGATAAACTGGTT‑GCCCGTATGCCGCCAGTGGTCAGCGAGGCTACAGCCTATGCCGCCGTGTTTAAGTTTGACTGGT‑TCT‑CTGCCACCGGCACCGCCATTCTGTTTGCTGCACTGCTCTCGATTGTCTGGCTGAAGATGAAAACGTCTGACGCTATCAGCACCTTCGGCAGCACGCTg                                                                                                                                                                                                                      >  1:57528/1‑250 (MQ=255)
                                     ccgTTTAAA‑GCCCTGTCCCCATCGGGGGGCGCGTTGTATGAGTGGGGGATCAATATCCCGGTGCCGTACCTCGATAAACGGGTG‑GCCCGTATGCCGCCGTTGGTCGGGGAGGCTACAGCCTATGCCGCCTTGTTTAAGTTTGACTGGT‑TCG‑CTGCCCCCGGCACCGCCATTCTGTTTGCTGCACTGCTCTCGATTGTCTGGCTGAAGATGAAAACGTCTGACGCTATCAGCACCTTCGGCAGCACGCTGaa                                                                                                                                                                                                                    <  1:307671/251‑1 (MQ=255)
                                        tttAAA‑GCCCTGTTCGCATCGGGTGGCGCGCTGTATGAGTGGGGGATCAATATTCCGGTGCCGTACCTCGATAAACTGGCT‑GCCCGTATGCCGCCAGTGGACAGCGAGGCTACAGCCTATGCCGCCGTGTTTAAGTTTGACTGGT‑TCT‑ATGCCACCGGCACCGCCATTCTGTTTGCTGCACTGCTCTCGCTTGTCTGGCTGAAGATGAACCCGTCTGACGCTATCAGCCCCTTCGGCAGCACGCTGaa                                                                                                                                                                                                                    >  1:300763/1‑248 (MQ=255)
                                                  cTGTTCGCATCGGGTGGCGCGCTGTATGAGTGGGTGATCAATATTCCGGTGCCGTACCTCGATAAACTGGTT‑GCCCGTATGCCGCCAGTGGTCAGCGAGGCTACAGCCTATGCCGCCGTGTTTAAGTTTGACTGGT‑TCT‑CTGCCACCGGCACCGCCATTCTGTTTGCTGCACTGCTCTCGATTGTCTGGCTGAAGATGAAACCGTCTTACGCTATCAGCACCTTCGGCAGCACGCTGAAAAAACTGGctct                                                                                                                                                                                                        >  1:167850/1‑251 (MQ=255)
                                                             ggggggCGCTCTGTATGAGTGGGTGATCAATATCCCGGTGCCGTACCTCGAAAAACTGGTT‑CCCCGTATGCCGCCGGTGGTCAGCGAGGCTACAGCCTATGCCGCCGTGTTTAAGTTTGACGGGT‑TCT‑CTGCCCCCGGCACCGCCATTCTGTTTGCTGCACTGCTCTCGATTGTCTGGCTGAAGATGAAAACGTCTGACGCTATCAGCACCTTCGGCAGCACGCTGAAAGAACTGGCTCTGCCCATCTACt                                                                                                                                                                                             <  1:259490/247‑1 (MQ=255)
                                                             gggTGGCGCGCTGTATGAGTGGGTGATCAATATTCCGGTGCCGTACCTCGATAAACTGGTT‑GCCCGTATGCCGCCAGTGGTCAGCGAGGCTACAGCCTATGCCGCCGTGTTTAAGTTTGACTGGT‑TCT‑CTGCCACCGGCACCGCCATTCTGTTTGCTGCACTGCTCTCGATTGTCTGGCTGAAGATGAAAACGTCTGACGCTATCAGCACCTTCGGCAGCACGCTGAAAGAACTGGCTCTGCCCATCTACt                                                                                                                                                                                             >  2:22804/1‑251 (MQ=255)
                                                                   cgcgcTGTATGAGTGGGTGATCAATATTCCGGTGCCGTACCTCGATAAACTGGTT‑GCCCGTATGCCGCCAGTGGTCAGCGAGGCTACAGCCTATGCCGCCGTGTTTAAGTTTGACTGGT‑TCT‑CTGCCACCGGCACCGCCATTCTGTTTGCTGCACTGCTCTCGATTGTCTGGCTGAAGATGAAAACGTCTGACGCTATCAGCACCTTCGGCAGCACGCTGAAAGAACTGGCTCTGCCCATCTACTCCATCg                                                                                                                                                                                       >  2:251496/1‑251 (MQ=255)
                                                                                        cAATATCCCGGTGCCGTTCCTCGATAAACTGGTG‑CCCCGTATGCCGCAAGGGGTCAGCGAGGCTACAGCTTATGCGGCCGTGTTTAATTTTGCCGGTT‑TCC‑CGGCCACCGGCACCGCCATTCTGTTTGCTGCACTGCTCTCGATTGTCTGGCTGAAGATGAAACCGTCTGACGCTATCAGCACCTTCGGCAGCACGCTGAAAGAACTGGCTCTGCCCATCTACTCCATCGGTATGGTGCTGGCATTCGCCt                                                                                                                                                                  <  1:239319/251‑1 (MQ=255)
                                                                                                           cTCGATAAACTGGTT‑GCCCGTATGCCGCCAGTGGTCAGCGAGGCTACAGCCTATGCCGCCGTGTTTAAGTTTGACTGGT‑TCT‑CTGCCACCGGCACCGCCATTCTGTTTGCTGCACTGCTCTCGATTGTCTGGCTGAAGATGAAACCGTCTGACGCTATCAGCACCTTCGGCAGCACGCTGAAAGAACTGGCTCTGCCCATCTACTCCATCGGCATGGTGCTGGCATTCGCCTTTATTTCGAACTATTCCgg                                                                                                                                               >  1:28970/1‑251 (MQ=255)
                                                                                                                 aaaCTGGTT‑GCCCGTATGCCGCCAGTGGTCAGCGAGGCTACAGCCTATGCCGCCGTGTTTAAGTTTGACTGGT‑TCT‑CTGCCACCGGCACCGCCATTCTGTTTGCTGCACTGCTCTCGATTGTCTGGCTGAAGATGAAACCGTCTGACGCTATCAGCACCTTCGGCAGCACGCTGAAAGAACTGGCTCTGCCCATCTACTCCATCGGTATGGTGCTGGCATTCGCCTTTATTTCGAACTATTCCGGCCTGtc                                                                                                                                         >  2:115111/1‑251 (MQ=255)
                                                                                                                       gccggcccttaggccgccGGTGGTCAGCGGGGCTACCGCTTATGCCGCCGTGTTTAATTTTGACTGTT‑TCT‑CGGCCACCGGCACCGCCATTCTGTTTGCTGCACTGCTCTCGATTGTCTGGCTGAAGATGAAACCGTCTGACGCTATCAGCACCTTCGGCAGCACGCTGAAAGAACTGGCTCTGCCCATCTACTCCATCGGTATGGTGCTGGCATTCGCCTTTATTTCGAGCTATTCCGGACTGtcatca                                                                                                                                     <  1:234462/238‑1 (MQ=255)
                                                                                                                         t‑GCCCGTATGCCGCCAGTGGTCAGCGAGGCTACAGCCTATGCCGCCGTGTTTAAGTTTGACTGGT‑TCT‑CTGCCACCGGCACCGCCATTCTGTTTGCTGCACTGCTCTCGATTGTCTGGCTGAAGATGAAAACGTCTGACGCTATCAGCACCTTCGGCAGCACGCTGAAAGAACTGGCTCTGCCCATCTACTCCATCGGTATGGTGCTGGCATTCGCCTTTATTTCGAACTATTCCGGACTGTCATCAacac                                                                                                                                 >  1:48528/1‑251 (MQ=255)
                                                                                                                         t‑GCCCGTATGCCGCCAGTGGTCAGCGAGGCTACAGCCTATGCCGCCGTGTTTAAGTTTGACTGGT‑TCT‑CTGCCACCGGCACCGCCATTCTGTTTGCCGCACTGCTCTCGATTGTCTGGCTGAAGATGAAACCGTCTGACGCTATCAGCACCTTCGGCAGCACGCTGAAAGAACTGGCTCTGCCCATCTACTCCATCGGTATGGTGCTGGCATTCGCCTTTATTTCGAACTATTCCGGACTGTCATCAacac                                                                                                                                 >  2:93477/1‑251 (MQ=255)
                                                                                                                           gCCCGTCTGCCGCCAGTGGTCAGCGAGGCTACAGCCTATGCCGCCGTGCTTAAGTTTGACTGGT‑TCT‑CTGCCACCGGCACCGCCATTCTGTTTGCTGCACTGCTCTCGATTGTCTGGCTGAAGATGAAACCGTCTGACGCTATCAGCCCCTTCGGCAGCACGCTGAAAGAACTGGCTCTGCCCATCTACTCCATCGGTATGGTGCTGGCATTCGCATTTATTTCGAACTATTCCGGACTGTCATCAACACt                                                                                                                                >  2:195374/1‑251 (MQ=255)
                                                                                                                                   gccgctggtgttcggCGAGGCGACAGCCTAGCCCGCCGTGTTTAAGTTTG‑CTTGTGTCT‑CTGCCTCCGACACCGCCATTCTGTTTGCTGCACTGCTCTCGATTGTCTGGCTGAAGATGAAAACGTCTGACGCTATCAGCACCTTCGGCAGCACGCTGAAAGAACTTTCTCGGCCCATCTTCTCCATCGGTATGGTGCTGTCATTCGCCTTTATTTCGAACTATTCCGGACTGTCATCAACACTGGCGCTgg                                                                                                                        <  1:22462/237‑1 (MQ=255)
                                                                                                                                    ccgccAGTGGTCAGCGAGGCTACAGCCTATGCCGCCGTGTTTAAGTTTGACTGGT‑TCT‑CTGCCACCGGCACCGCCATTCTGTTTGCTGCACTGCTCTCGATTGTCTGGCTGAAGATGAAACCGTCTGACGCTATCAGCACCTTCGGCAGCACGCTGAAAGAACTGGCTCTGCCCATCTACTCCATCGGTATGGGGATGGCATTCGCCTTTATTTCGAACTATTCCGGACTGTCATCAACACTGGCGCTGGc                                                                                                                       >  1:159680/1‑251 (MQ=255)
                                                                                                                                         aGTGGTCAGCGAGGCTACAGCCTATGCCGCCGTGTTTAAGTTTGACTGGT‑TCT‑CTGCCACCGGCACCGCCATTCTGTTTGCTGCACTGCTCTCGATTGTCTGGCTGAAGATGAAACCGTCTGACGCTATCAGCACCTTCGGCAGCACGCTGAAAGAACTGGCTCTGCCCATCTACTCCATCGGTATGGTGCTGGCATTCGCCTTTATTTCGAACTATTCCGGACTGTCATCAACACTGGCGCTGGCACTgg                                                                                                                  >  1:61589/1‑251 (MQ=255)
                                                                                                                                                     gccacaaGCCGATGCCGCCGTTTTTAAGTTTGTCGGTT‑TCT‑CTCCCCCCGGCACCGCCATTCTGTTTGCTGCACTGCTCTCGATTGTCTGGCTGAAGATGAAAACGTCTGACGCTATCAGCACCTTCGGCAGCACGCTGAAAGAACTGGCTCTGCCCATCTACTCCATCGGTATGGTGCTGGCATTCGCCTTTATTTCGAACTATTCCGGACTGTCATCAACACTGGCGCTGGCACTGGCGCACACCGGTc                                                                                                      <  1:270343/245‑1 (MQ=255)
                                                                                                                                                      gctacacccagtcccgccggttttAAGTTTCACTTTT‑TCT‑CTGCCCCCGCCTCCGCCATTCTGTTTGCTGCACTGCTCTCGATTGTCTGGCTGAAGATGAAAACGTCTGACGCTATCAGCACCTTCGGCAGCACGCTGAAAGAACTGGCTCTGCCCAGCTACTCCATCGGTATGGTGCTGGCATTCGCCTGTATTTCGAACTATTCCGGACTGTCATCAACACTGGCGCTGGCACTGGCGCACACCGGTCa                                                                                                     <  2:136486/230‑1 (MQ=255)
                                                                                                                                                                  gccgccGTGTTTAAGTTTGACTGGT‑TCT‑CTGCCACCGGCACCGCCATTCTGTTTGCTGCACTGCTCTCGATTGTCTGGCTGAAGATGAAACCGTCTGACGCTATCAGCACCTTCGGCAGCACGCTGAAAGAACTGGCTCTGCCCATCTACTCCATCGGTATGGTGCTGGCATTCGCCTTTATTTCGAACTATTCCGGACTGTCATCAACACTGGCGCTGGCAATGGCGCACACCGGTCATGCATTCACctt                                                                                         >  1:228194/1‑251 (MQ=255)
                                                                                                                                                                   ccgctgTGTTTAATTTTCGCTGGT‑TCTTCT‑CCCCCGGCCCCGCCATTCTGTTTGCTGCACTGCTCTCGATTGTCTGGCTGAAGATGAAAACGTCTGACGCTATCAGCCCCTTCGGCAGCACGCTGAAAGAACTGGCTCTGCCCATCTACTCCATCGGTATGGTGCTGGCATTCGCCTTTATTTCGAACTATTCCGGACTGTCATCAACACTGGCGCTGGCACTGGCGCACACCGGTCATGCATTCGCcttc                                                                                        <  1:252389/251‑1 (MQ=255)
                                                                                                                                                                       cgTGTTTAAGTTTGACTGGT‑TCT‑CTGCCACCGGCACCGCCATTCTGTTTGCTGCACTGCTCTCGATTGTCTGGCTGAAGATGAAACCGTCTGACGCTATCAGCACCTTCGGCAGCACGCTGAAAGAACTGGCTCTGCCCATCTACTCCATCGGTATGGTGCTGGCATTCGCCTTTATTTCGAACTATTCCGGCCTGTCATCAACACTGGCGCTGGCACTGTCGCCCACCGGTCATGCATTCACCttcttcc                                                                                    >  2:67269/1‑250 (MQ=255)
                                                                                                                                                                                  ttGACTGGT‑CCT‑CTGCCCCCGGCACCGCCATTCTGTTTGCTGCACTGCTCTCGATTGTCTGGCTGAAGATGAAAACGTCTGACGCTATCAGCACCTTCGGCAGCACGCTGAAAGAACTGGCTCTGCCCATCTACTCCATCGGTATGGTGCTGGCATTCGCCTTTATTTCGAACTATTCCGGACTGTCATCAACACTGGCGCTGGCACTGGCGCACACCGGTCATGCATTCACCTTCTTCTCGCCGTTCCt                                                                          <  1:250458/250‑1 (MQ=255)
                                                                                                                                                                                    gACTGGT‑TCT‑CTGCCACCGGCACCGCCATTCTGTTTGCTGCACTGCTCTCGATTGTCTGGCTGAAGATGAAAACGTCTGACGCTATCAGCACCTTCGGCAGCACGCTGAAAGAACTGGCTCTGCCCATCTACTCCATCGGTATGGTGCTGGCATTCGCCTTTATTTCGAACTATTCCGGACTGTCATCAACACTGGCGCTGGCACTGGCGCACACCGGTCATGCATTCACCTTCTTCTCGCCGTTCCTCgg                                                                       <  1:22804/251‑1 (MQ=255)
                                                                                                                                                                                                 cgcccgcGGCACCGCCATTCTGTTTGCTGCACTGCTCTCGATTGTCTGGCTGAAGATGAAACCGTCTGACGCTATCAGCACCTTCGGCAGCACGCTGAAAGAACTGGCTCTGCCCATCTACCCCATCGGTATGGTGCTGGCATGCGCCTTTATTTCGAACTATGCCGGACTGTCATCAACACTGGCGCGGGCACTGGCGGACACCGGTCATGCATGCACCTTCTTCTCGCCGTTCCTCggctggggggggg                                                            <  2:127264/245‑8 (MQ=255)
                                                                                                                                                                                                  gCCACCGGCACCGCCATTCTGTTTGCTGCACTGCTCTCGATTGTCTGGCTGAAGATGAAACCGTCTGACGCTATCAGCACCTTCGGCAGCACGCTGAAAGAACTGGCTCTGCCCATCTACTCCATCGGTATGGTGCTGGCATTCGCCTTTATTTCGAACTATTCCGGACTGTCATCAACACTGGCGCTGGCACTGGCGCACACCGGTCATGCATTCACCTTCTTCTCGCCGTTCCTCGGCTGGCTGGGGGt                                                           <  1:257751/251‑1 (MQ=255)
                                                                                                                                                                                                   ccACCGGCACCGCCATTCTGTTTGCTGCACTGCTCTCGATTGTCTGGCTGAAGATGAAACCGTCTGACGCTATCAGCACCTTCGGCAGCACGCTGAAAGAACTGGCTCTGCCCATCTACTCCATCGGTATGGTGCTGGCATTCGCCTTTATTTCGAACTATTCCGGACTGTCATCAACACTGGCGCTGGCACTGGCGCACACCGGTCATGCATTCACCTTCTTCTCGCCGTTCCTCGGCTGGCTGGGGGt                                                           >  1:185125/1‑250 (MQ=255)
                                                                                                                                                                                                                cATTCTGTTTGCTGCACTGCTCTCGATTGTCTGGCTGAAGATGAAACCGTCTGACGCTATCAGCACCTTCGGCAGCACGCTGAAAGAACTGGCTCTGCCCATCTACTCCATCGGTATGGTGCTGGCATTCGCCTTTATTTCGAACTATTCCGGACTGTCATCAACACTGGCGCTGGCACTGGCGCACACCGGTCATGCATTCACCTTCTTCTCGCCGTTCCTCGGCTGGCTGGGGGTATTCCTGACCGGGt                                             >  2:39103/1‑251 (MQ=255)
                                                                                                                                                                                                                cATTCTGTTTGCTGCACTGCTCTCGATTGTCTGGCTGAAGATGAAACCGTCTGACGCTATCAGCACCTTCGGCAGCACGCTGAAAGAACTGGCTCTGCCCATCTACTCCATCGGTATGGTGCTGGCATTCGCCTTTATTTCGAACTATTCCGGACTGTCATCAACACTGGCGCTGGCACTGGCGCACACCGGTCATGCATTCACCTTCTTCTCGCCGTTCCTCGGCTGGCTGGGGGTATTCCTGACCGGGt                                             >  1:269658/1‑251 (MQ=255)
                                                                                                                                                                                                                      gTTTGCTGCACTGCTCTCGATTGTCTGGCTGAAGATGAAACCGTCTGACGCTATCAGCACCTTCGGCAGCACGCTGAAAGAACTGGCTCTGCCCATCTACTCCATCGGTATGGTGCTGGCATTCGCCTTTATTTCGAACTATTCCGGACTGTCATCAACACTGGCGCTGGCACTGGCGCACACCGGTCATGCATTCACCTTCTTCTCGCCGTTCCTCGGCTGGCTGGGGGTATTCCTGACCGGGTCgg                                          <  1:124832/248‑1 (MQ=255)
                                                                                                                                                                                                                      gTTTGCTGCACTGCTCTCGATTGTCTGGCTGAAGATGAAACCGTCTGACGCTATCAGCACCTTCGGCAGCACGCTGAAAGAACTGGCTCTGCCCATCTACTCCATCGGTATGGTGCTGGCATTCGCCTTTATTTCGAACTATTCCGGACTGTCATCAACACTGGCGCTGGCACTGGCGCACACCGGTCATGCATTCACCTTCTTCTCGCCGTTCCTCGGCTGGCTGGGGGTATTCCTGACCGGGTCgg                                          <  1:187337/248‑1 (MQ=255)
                                                                                                                                                                                                                          gctgcACTGCTCTCGATTGTCTGGCTGAAGATGAAACCGTCTGACGCTATCAGCACCTTCGGCAGCACGCTGAAAGAACTGGCTCTGCCCATCTACTCCATCGGTATGGTGCTGGCATTCGCCTTTATTTCGAACTATTCCGGACTGTCATCAACACTGGCGCTGGCACTGGCGCACACCGGTCATGCATTCACCTTCTTCTCGCCGTTCCTCGGCTGGCTGGGGGTATTCCTGACCGGGTCGGATACCTc                                   >  1:132136/1‑251 (MQ=255)
                                                                                                                                                                                                                            tgcACTGCTCTCGATTGTCTGGCTGAAGATGAAAACGTCTGACGCTATCAGCACCTTCGGCAGCACGCTGAAAGAACTGGCTCTGCCCATCTACTCCATCGGTATGGTGCTGGCATTCGCCTTTATTTCGAACTATTCCGGACTGTCATCAACACTGGCGCTGGCACTGGCGCACACCGGTCATGCATTCACCTTCTTCTCGCCGTTCCTCGGCTGGCTGGGGGTATTCCTGACCGGGTCGGATACCTCAt                                 >  1:168622/1‑251 (MQ=255)
                                                                                                                                                                                                                                 cgCTCTCGATTGTCTGGCTGAAGATGAAACCGTCTGACGCTATCAGCACCTTCGGCAGCACGCTGAAAGAACTGGCTCTGCCCATCTACTCCATCGGTATGGTGCTGGCATTCGCCTTTATTTCGAACTATTCCGGACTGTCATCAACACTGGCGCTGGCACTGGCGCACACCGGTCATGCATTCACCTTCTTCTCGCCGTTCCTCGGCTGGCTGGGGGTATTCCTGACCGGGTCGGATACCTCATCTAAc                            <  1:40024/250‑1 (MQ=255)
                                                                                                                                                                                                                                  gCTCTCGTTTGTCTGGCTTAAGATGAAACCGTCTGACGCTATCAGCCCCTTCGGCAGCACGCTGAAAGAACTGGCTCTGCCCATCTACGCCATCGGTATGGTGCTGGCATTCGCCTTGATTTCGAACTATTCCGGACTGTCATCAACACTGGCGCTGGCGCTGGCGCACACCGGTCATGCATTCACCTTCTTCTCGCCTTTCCTCGGCTGGCTGGGGGTATTCCTGACCGGGTCGGATACCTCATCTaa                             <  2:267216/249‑1 (MQ=255)
                                                                                                                                                                                                                                            gTCTGGCTGAAGATGACACCGTCTGACGCTATCAGCACCTTCGGGAGCACGCTGAAAGAACTGGCGCTGCCCATCTACTCCATCGGTATGGTGCTGGCATTCGCCTTTATTTCGAACTATTCCGGACTGTCATCAACACTGGCGCTGGCACTGGCGCACACCGGTCATGCATTCACCTTCTTCTCGCCGTTCCTCGGCTGGCTGGGGGTATTCCTGACCGGGTCGGATACCTCATCTAACGCCCTGtgagc                 >  2:161563/1‑247 (MQ=255)
                                                                                                                                                                                                                                                    gAAGATGAAACCGTCTGACGCTATCAGCACCTTCGGCAGCACGCTGAAAGAACTGGCTCTGCCCATCTCCTCCATCGGTATGGTGCTGGCATTCGCCTTTATTTCGAACTATTCCGGACTGTCATCAACACTGGCGCTGGCACTGGCGCACACCGGTCATGCATTCACCTTCTTCTCGCCGTTCCTCGGCTGGCTGGGGGTATTCCTGACCGTGTCGGATACCGCATCTAACGCCCTGTTCGCCGCGCTg          <  2:47661/250‑1 (MQ=255)
                                                                                                                                                                                                                                                           aaaCCGTCTGACGCTATCAGCACCTTCGGCAGCACGCTGAAAGAACTGGCTCTGCCCATCTACTCCATCGGTATGGTGCTGGCATTCGCCTTTATTTCGAACTATTCCGGACTGTCATCAACACTGGCGCTGGCACTGGCGCACACCGGTCATGCATTCACCTTCTTCTCGCCGTTCCTCGGCTGGCTGGGGGTATTCCTGACCGGGTCGGATACCTCATCTAACGCCCTGTTCGCCGCGCTGCAAGCCAc  <  1:215186/251‑1 (MQ=255)
                                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                       
GTTCCTGACAGCTACCGTAACACTGTGGAGTATCCCGCCGTTTAAA‑GCCCTGTTCGCATCGGGTGGCGCGCTGTATGAGTGGGTGATCAATATTCCGGTGCCGTACCTCGATAAACTGGTT‑GCCCGTATGCCGCCAGTGGTCAGCGAGGCTACAGCCTATGCCGCCGTGTTTAAGTTTGACTGGT‑TCT‑CTGCCACCGGCACCGCCATTCTGTTTGCTGCACTGCTCTCGATTGTCTGGCTGAAGATGAAACCGTCTGACGCTATCAGCACCTTCGGCAGCACGCTGAAAGAACTGGCTCTGCCCATCTACTCCATCGGTATGGTGCTGGCATTCGCCTTTATTTCGAACTATTCCGGACTGTCATCAACACTGGCGCTGGCACTGGCGCACACCGGTCATGCATTCACCTTCTTCTCGCCGTTCCTCGGCTGGCTGGGGGTATTCCTGACCGGGTCGGATACCTCATCTAACGCCCTGTTCGCCGCGCTGCAAGCCAC  >  NC_000913/3778319‑3778816

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑