Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	NC_000913	1,293,268	IS5 (–) +4 bp	intergenic (‑346/‑256)	hns ← / → tdk	DNA‑binding transcriptional dual regulator H‑NS/thymidine/deoxyuridine kinase

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	1293268 =	0 (0.000)	20 (0.660)	19/488	0.5	100%	intergenic (‑346/‑259)	hns/tdk	DNA‑binding transcriptional dual regulator H‑NS/thymidine/deoxyuridine kinase
	?	NC_000913	= 2290113	NA (NA)					noncoding (1/1195 nt)	IS5	repeat region
*	?	NC_000913	= 1293271	0 (0.000)	14 (0.460)	13/490	0.9	100%	intergenic (‑349/‑256)	hns/tdk	DNA‑binding transcriptional dual regulator H‑NS/thymidine/deoxyuridine kinase
	?	NC_000913	2101749 =	NA (NA)					noncoding (1195/1195 nt)	IS5	repeat region

AAAACTTCGATTGTCATGATGAGCATAATGTATCGCATGAAAAGCGGGAACATAATGAAGTCTGACACCAGCCAGGCTAAGCCTCAAGCACAGGTCAATATCTTCACAGTACATAAAGTAACCTTGATCGAAGCCATTTACACGCACAAAATCTGAAAAACGTACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCAGTTTTTAGTGAGATCTCTCCCACTGACGTATCATTTGGTCCGCCCGAAACAGGTTGGCCAGCGTGAATAACATCGCCAGTTGGTTATCGT  >  NC_000913/2101507‑2101980                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                        aaaaCTTCGATTGTCATGATGAGCATAATGTATCGCATGAAAAGCGGGAACATAATGAAGTCTGACACCAGCCAGGCTAAGCCTCAAGCACAGGTCAATATCTTCACAGTACATAAAGTAACCTTGATCGAAGCCATTTACACGCACAAAATCTGAAAAACGTACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGc                                                                                                                                                                                                                                 >  1:263083/1‑251 (MQ=255)                  gatgaGCATAATGTATCGCATGAAAAGCGGGAACATAATGAAGTCTGACACCAGCCAGGATAAGCCTCAAGCACAGGTCAAGATCTTCACAGTACATAAAGTAACCTTGATCGAAGCCATTTACACGCACAAAATCTGAAAAACGTACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGAGACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAATCGGGGAAAtt                                                                                                                                                                                                                <  2:107248/251‑1 (MQ=255)                                             cGGGAACATAATGAAGTCTGACACCAGCCAGGCTAAGCCTCAAGCACAGGTCAATATCTTCACAGTACATAACGTAACCTTGCTCGAAGCCATTTACACGCACAAAATCTGAAAAACGTACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTCGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGtcatttcatt                                                                                                                                                                                     >  1:144488/1‑251 (MQ=255)                                                                  cacCAGCCAGGCTAAGCCTCAAGCACAGGTCAATATCTTCACAGTACATAAAGTAACCTTGATCGAAGCCATTTACACGCACAAAATCTGAAAAACGTACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGttt                                                                                                                                                                          <  1:147446/241‑1 (MQ=255)                                                                  cacCAGCCAGGCTAAGCCTCAAGCACAGGTCAATATCTTCACAGTACATAAAGTAACCTTGATCGAAGCCATTTACACGCACAAAATCTGAAAAACGTACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGttt                                                                                                                                                                          >  2:147446/1‑241 (MQ=255)                                                                                                                cATAAAGTAACCTTGATCGAAGCCATTTACACGCACAAAATCTGAAAAACGTACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTTTTCATGTTTGAGCCTATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTttcttc                                                                                                                  <  1:283521/251‑1 (MQ=255)                                                                                                                       tAACCTTGATCGAAGCCATTTACACGCACAAAATCTGAAAAACGTACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGAcc                                                                                                                          <  2:89210/236‑1 (MQ=255)                                                                                                                       tAACCTTGATCGAAGCCATTTACACGCACAAAATCTGAAAAACGTACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGAcc                                                                                                                          >  1:89210/1‑236 (MQ=255)                                                                                                                                          ttACACGCACAAAATCTGAAAAACGTACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGGGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGCTTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGccctt                                                                                                            >  2:63561/1‑228 (MQ=255)                                                                                                                                          ttACACGCAAAAAATCTGAAAAACGTACCAGCATAAATGATCCTGCGCACCAATCAACAACAGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCAGTTCTTCATGTTTGAGCCGATTTTTGCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTGCTTCGCCAtt                                                                                                            <  1:63561/231‑1 (MQ=255)                                                                                                                                              acGCACAAAATCTGAAAAACGTACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCAGttttt                                                                                      >  2:22380/1‑249 (MQ=255)                                                                                                                                                              aaaCGTACCAGCATAATTGATCCTGCGCACCAATCAACAAACGTCTCCGCATTGTTACTTTCTTTAGGAATTTTTGTTTTATTAAGGCAGGTGCGCATACGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCCTTTCTTCCTGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTCTTTAGACCGTTTCTTCGCCCTTTACGGCGTTATCCCCAGTTTTTAGTGTGCTCTCTCCCaat                                                                    >  1:176671/1‑249 (MQ=17)                                                                                                                                                                   tacaaGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCAGTTTTTATGGAGATCTATCCCACTGACGt                                                               >  2:171877/5‑251 (MQ=255)                                                                                                                                                                            aaaTGATCCTGCGCACCAATCAACAACCGAATCAGACTAGACACTTTCTTTAGGCATTTTTGTTTTATCAAGGAAGGAGCGAATAAGAGGGGCAATTCTTCTCGGCTGACTCAGTCATTTCATTTATTCATGTTTGAGCCGATTTTTTCTCAAGTAAATGCCTTGAATAAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCAGTTTTTAGTGAGATCTCTCCCACTGACGTATCATATgg                                                      >  2:104555/1‑251 (MQ=17)                                                                                                                                                                                             aaTCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCAGTTTTGAGTGAGATCTCTCCCACTGACGTATCATTTGGTCCGCCCGAAACAGGtt                                     <  2:247887/251‑1 (MQ=255)                                                                                                                                                                                                               aaTAGACACTTTCCTTACGACTCTTTGTTATATTAAGGCAGGTGAGAATAAGCGGGCAAATACTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATCCCTTGTATCAGCCTATTTAGCCCGTTTCTTCGCCATTTAAGGCCTTATCCCCAGTTTTTAGTGAGATCTCTCCCACTCACGTATCATTTGGTCCGCCCGAACCAGGTTGGCCAGCGTGAATAACAt                   >  2:144389/1‑251 (MQ=11)                                                                                                                                                                                                                    aTACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTTGGCTGAATCAGTAATTTCATTTCTTCATGTTTGAGCAGATTTTTTCTCCAGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCAATTTAAGGCGTTATCCACAGTTTTTAGTGAGATCTCTCCCACTGACGTATCATTTGGTCCGCCCGAAACAGGTTGGCCA‑AGTGAATAACATcacccg             >  1:175356/1‑246 (MQ=14)                                                                                                                                                                                                                                ggAATTTTTGTTTTATTAAGGAAGGTTCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCAGTTTTTAGTGAGATCTCTCCCACTGACGTATCATTTGGTCCGCCCGAAACAGGTTGGCCAGCGTGAATAACATCGCCAGTTGGTTATCGt  <  1:179901/251‑1 (MQ=37)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                        AAAACTTCGATTGTCATGATGAGCATAATGTATCGCATGAAAAGCGGGAACATAATGAAGTCTGACACCAGCCAGGCTAAGCCTCAAGCACAGGTCAATATCTTCACAGTACATAAAGTAACCTTGATCGAAGCCATTTACACGCACAAAATCTGAAAAACGTACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCAGTTTTTAGTGAGATCTCTCCCACTGACGTATCATTTGGTCCGCCCGAAACAGGTTGGCCAGCGTGAATAACATCGCCAGTTGGTTATCGT  >  NC_000913/2101507‑2101980

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.