| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,296,381 | +GC | intergenic (+587/+55) | gltP → / ← yjcO | glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,296,380 | 1 | . | C | 96.0% | 64.3 / NA | 27 | intergenic (+586/+56) | gltP/yjcO | glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO |
| Reads supporting (aligned to +/- strand): ref base . (1/0); major base C (13/11); minor base A (1/0); total (16/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.70e-01 | |||||||||||
| * | NC_000913 | 4,296,380 | 2 | . | G | 92.6% | 71.1 / ‑0.7 | 27 | intergenic (+586/+56) | gltP/yjcO | glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO |
| Reads supporting (aligned to +/- strand): ref base . (2/0); new base G (14/11); total (16/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.99e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.48e-01 | |||||||||||
AGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑A‑‑C‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGTACTCGGAATAACCGGTTCGGGAAATTGCAGAGC > NC_000913/4296203‑4296611 || aGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑ACGC‑C‑GCATCCGACATCAATGCCTGCTGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAAt > 1:237940/1‑251 (MQ=255) ccTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑ACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAAtttt > 1:171282/1‑251 (MQ=25) gCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑ACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCaa > 1:298401/1‑250 (MQ=255) gCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTAGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACTGCGCAATAGCCTGATATAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑ACGC‑C‑GCATCCGACATCATTGCCTGATGCGACGCTTGCCGCGTCTAATCAGTCCTATCTTAACCGTTGGATAATTTTTcca > 1:191345/1‑248 (MQ=11) caTAGCCTGATTTTGCGTTATTTTGTAGGGCGGGAGAGGCGATTACGCCGCATCCTACATCAACGCCTGATGCGACGCTTACCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑ACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACAccc < 2:247000/250‑1 (MQ=14) aaTAGCATGCTTTAGCGTGATTTTGTAGGTCGGCTAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACCGCGCAATATCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑ACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCCATCTTAAAAGTTGGTTAATTTTTCAAACTATTCACAccc > 2:252895/1‑251 (MQ=11) gCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑ACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTa < 2:171282/251‑1 (MQ=255) tGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑ACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCTTTGGTTAATTTTTCAAACTCTTCACACCCGGTATc > 2:2554/1‑250 (MQ=255) tAGCGTGATTTTGTAGGTCGGATAAGTCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑ACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCCCACCCGGTATCAAAcc < 1:252895/250‑1 (MQ=255) ttttGTAGGTCGGATCAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACTCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAATGCGTTT‑ACGC‑C‑GCATCCGACATCATTGCCTGATGAGACGCTTTCCGCGTCTTATCAGTCCTATCTTAACCGTAGGTTAATTTTTTAAGCTCTTCCGATCCGGTCTCAAACCCTTCCAttc > 2:55808/1‑249 (MQ=14) aGGTCGGATAAGGTGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑ACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCa < 2:47016/251‑1 (MQ=255) gTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGTTTAACGCGGCTTAGCAGGCCTTCGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGATT‑ACGC‑C‑GCATCCGTCATCAATGCCTTATGCGTCGCTTGCTGCGTCTTATCAGTCCTATCTTAACCGTTGGTTAATTTGTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAgt < 1:181860/251‑2 (MQ=17) tAAGACGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTCTCAGGCCTACGACAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTCAG‑A‑C‑GCATCCGAAATCAATGCCTGAAGCGACGCTTGCCGCGTCTTATAAGGACTATCTTAACTGGTGGGTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGATCAGGTTccac > 2:77723/1‑248 (MQ=17) aGGCGTTTATGCCGCATCAGCAATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTAAGCCAGACAGAGCAATAGCCTGATTTAGCGTGATTTTGTAGGACGGATAAGGCGGTT‑A‑‑CGCTGTATCCGCCATCAATGACGGATGCGACGCCTGCCACGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTTTATCTCTTCACACACGGTATCAAACCCTTcccaaaatattaggtttaaca > 2:42831/1‑231 (MQ=9) ttATGCCGCATCCGGCATCAACGCCTGATGCGACGCTTAACGCTTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑ACGC‑C‑GCAGCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCTGTGCa < 2:152795/250‑1 (MQ=18) aTGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑ACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAgcg < 1:81933/251‑1 (MQ=255) aTCAACGCCTGATGTGATGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTATCGTGATTTTGTAGGGCGGATAAGGCGTTT‑ACGC‑C‑GCATCCGACATCAAGGCCTGATGCGACGCTTTCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGttc < 2:298401/251‑1 (MQ=21) tCAACGCCTGCTGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑ACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACCGCTCAGGTTCAACCAGTTCAGCGCCTTTTGCTTGttct > 2:216919/1‑251 (MQ=255) gCGACGATTAACGCGTCTTATCAGTCCTACGCCATACAGCGCAATAGCCTGATTTAGCGTGATTTGGTAGGTCGGATAAGGCGTGT‑ACGC‑C‑GCGTCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCATGCCTATCGTAACCGTGGGTTAATTTTTCAAACGCTTCACACCCGTTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGACGGGTGCTTGTTCTTCTCGATAAAAc < 2:274874/251‑1 (MQ=18) gCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑ACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACcttct > 2:242065/1‑251 (MQ=255) ttAACGCGTCTTATCAGGCCTACGCCAGACAGCGCACTAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑ACGC‑C‑GCATCCGACATCAATGCCTGATTCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACCCCCGGTATCAAACCCTTCCATAAAGCTCAGGTTCACCCATTGCAGCGCCTTTTGCTTGTTCTTCTCGTTAACACCttcttc > 2:261487/1‑251 (MQ=21) aaCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑ACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCAc > 1:64601/1‑251 (MQ=255) tAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑ACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTATTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCAAACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGAAGCGCCTTTTGATTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGTACTCGGa > 1:186160/1‑250 (MQ=37) cTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑ACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGTACTCGGAATAAc > 1:144532/1‑251 (MQ=255) tAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑ACGC‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGTACTCGGAATAACCGGtt < 1:231768/250‑1 (MQ=255) tGTAGGTCTGATAAGGCGTGT‑ACGC‑C‑GCATCCGACATCATTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAATCTCTTCACCCCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGTACTCGGAATAACCGGTTCGGGAAATTGc < 1:267916/250‑1 (MQ=39) ggTCGGATAAGGCGTTT‑AAGC‑C‑GCATACGACATCAATGAATGATGCGACCCTTGCCACGTCTTCTCAGGCCTATATTAAACGTTGGCTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTACACCCAGTGCAGCGCCCTTTCCTTGTTCTTCTCGATAACACCTTCTTCACCGTTTAAAACCATCATTCCCGCCAAGGAATCGAAATAACCGGTTCGGAAAATTGCAGAGc > 2:192782/1‑251 (MQ=255) || AGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑A‑‑C‑C‑GCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGTACTCGGAATAACCGGTTCGGGAAATTGCAGAGC > NC_000913/4296203‑4296611 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |