Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,964,675	C→T	G319D (GGT→GAT)	rhlB ←	ATP‑dependent RNA helicase RhlB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,964,675	0	C	T	100.0%	110.1 / NA	36	G319D (GGT→GAT)	rhlB	ATP‑dependent RNA helicase RhlB
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (14/22);  total (14/22)

AGGCGCAGCGGTTTTGGCAGATCGGTCATCAATGCGTCCGGATTGTATTTGCTTACCGGAATTGAGTGACCAATATAGGTCTCAATAGCAGGCAAATTCAATGCATACTCTTCACACGCCAGGCTGATAGAGTGACCGCTTGCGCCTGCGCGACCTGTACGACCAATACGGTGAACGTAATCTTCACAGTCATCGGGTAAATCGTAGTTAAAGACGTGCGTCACTGCCGGAATATGCAAACCACGCGCGGCAACGTCGGTGGCAACCAGAATATCCAGATCGCCACGGGTAAATTCATCAAGAATACGCAGACGTTTTTTCTGCGCGACATCGCCTGTCAATAAACCGACACGATGACCATCTGCTGCCAGGTGGCCCCAGATCTCTTCACAACGGTGTTTGGTGTTGGCGAAAATAATCGCTCTGTCTGGCCACTCTTCTTCGATCAGCGTTTGCAGCAAACGCATTTTTTC  >  NC_000913/3964435‑3964907                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                         aGGCGCAGCGGTTTTGGCAGATCGGTCATCAATGCGTCCGGATTGTATTTGCTTACCGGAATTGAGTGACCAATATAGGTCTCAATAGCAGGCAAATTCAATGCATACTCTTCACACGCCAGGCTGATAGAGTGACCGCTTGCGCCTGCGCGACCTGTACGACCAATACGGTGAACGTAATCTTCACAGTCATCGGGTAAATCGTAGTTAAAGACGTGCGTCACTGCCGGAATATGCAAATCACGCGCGGc                                                                                                                                                                                                                                >  1:238127/1‑251 (MQ=255)  tgcgcAGCGGGTTTGGACGATCGGTCATCAATGCGTCCGGATAGTATTTGCTTACCGGAATTGAGTGACCAATATAGGTCGCAATAACAGGCAAATTCAATGCATACTCTTCACACGCCAGGCTGATAGAGTGACCGCTTGCGCCTGCGCGACCTGTACGACCAATACGGTGAACGTAATCTTCACAGTCATCGGGTAAATCGTAGTTAAAGACGTGCGTAACTGCCGGAATATGCAAATCACGCGCGGc                                                                                                                                                                                                                                >  2:116736/2‑250 (MQ=255)                   aGATCGGTCATCAATGCGTCCGGATTGTATTTGCTTACCGGAATTGAGTGACCAATATAGGTCTCAATAGCAGGCAAATTCAATGCATACTCTTCACACGCCAGGCTGATAGAGTGACCGCTTGCGCCTGCGCGACCTGTACGACCAAGACGGTGAACGTAATCTTCACAGTCATCGGGTAAATCGTAGTTAAAGACGTGCGTCACTGCCGGAATATGCAAATCACGCGCGGCAACGTCGGTGGCAACCAg                                                                                                                                                                                                              <  2:145759/251‑1 (MQ=255)                       cGGTCATCAATGCGTCCGGATTGTATTTGCTTACCGGAATTGAGTGACCAATATAGGTCTCAATAGCAGGCAAATTCAATGCATACTCTTCACACGCCAGGCTGATAGAGTGACCGCTTGCGCCTGCGCGACCTGTACGACCAATACGGTGAACGTAATCTTCACAGTCATCGGGTAAATCGTAGTTAAAGACGTGCGTCACTGCCGGAATATGCAAATCACGCGCGGCAACGTCGGTGGCAACCAGAat                                                                                                                                                                                                           <  1:119324/250‑1 (MQ=255)                                 tGCGTCCGGATTGTATTTGCTTACCGGAATTGAGTGACCAATATAGGACTCAATAGCAGGCAAATTCAATGCATACTCTTCACACGCCAGGCTGATAGAGTGAACGCTTGCGCCTGCGCGACCTGTACGACCAATACGGTGCACGTAATCTTCACAGTCATCGGGTAAATCGTAGTTAAAGACGTGCGTCACTGCCGGAATATGCAAATCACGCGCGGCAACGTCGGTGGCAACCAGAATATCCAGATCGc                                                                                                                                                                                                >  1:154304/1‑251 (MQ=255)                                  gCGTCCGGATTGTATTTGCTTACCGGAATTGAGTGACCAATATAGGTCTCAATAGCAGGCAAATTCAATGCATACTCTTCACACGCCAGGCTGATAGAGTGACCGCTTGCGCCTGCGCGACCTGTACGACCAATACGGTGAACGTAATCTTCACAGTCATCGGGTAAATCGTAGTTAAAGACGTGCGTCACTGCCGGAATATGCAAATCACGCGCGTCAACGTCGGTGGCAACCAGAAGATCCAGATCGc                                                                                                                                                                                                <  1:203286/250‑1 (MQ=255)                                   cGTCCGGATTGTATTTGCTTACCGGAATTGAGTGACCAATATAGGTCTCAATAGCAGGCAAATTCAATGCATACTCTTCACACGCCAGGCTGATAGAGTGACCGCTTGCGCCTGCGCGACCTGTACGACCAATACGGTGAACGTAATCTTCACAGTCATCGGGTAAATCGTAGTTAAAGACGTGCGTCACTGCCGGAATATGCAAATCACGCGCGGCAACGTCGGTGGCAACCAGAATATCCAGATCGCCa                                                                                                                                                                                              <  1:249592/251‑1 (MQ=255)                                                        ccGGAATTGAGTGACCAATATAGGTCTCAATAGCAGGCGAATTCAATGCATACTCTTCACACGCCAGGCTGATAGAGTGACCGCTTGCGCCTGCGCGACCTGTACGACCAATACGGTGAACGTAATCTTCACAGTCATCGGGTAAATCGTAGTTAAAGACGTGCGTCACTGCCGGAATATGCAAATCACGCGCGGCAACGTCGGTGGCAACCAGAATATCCAGATCGCCACGGGTAAATTCATCAAGAAt                                                                                                                                                                          >  1:65504/1‑250 (MQ=255)                                                                gAGTGACCAAGATAGGTCTCAATAGCAGGCAAAATCAATGCATACTCGTCACACGCCAGGCTGATAGAGTGACCGCTTGCGCCTGCGCGACCTGTACGACCAATACGGTGAACGTAATCTTCACAGGCATCGGGTAAATCGTAGTTAAAGACGTGCGTCACTGCCGGAATATGCAAATCACGCGCGGCAACGTCGGTGGCAACCAGAATATCCAGATCGCCACGGGTAAATTCATCAAGAATACGCAGACg                                                                                                                                                                 <  1:157978/251‑1 (MQ=255)                                                                         atatAGGTCTCAATAGCAGGCAAATTCAATGCATACTCTTCACACGCCAGGCTGATAGAGTGACCGCTTGCGCCTGCGCGACCTGTACGACCAATACGGTGAACGTAATCTTCACAGTCATCGGGTAAATCGTAGTTAAAGACGTGCGTCACTGCCGGAATATGCAAATCACGCGCGGCAACGTCGGTGGCAACCAGAATATCCAGATCGCCACGGGTAAATTCATCAGGAATACGCAGACGTTTTTTCTg                                                                                                                                                        >  2:119309/1‑251 (MQ=255)                                                                                   cAATAGCAGGCAAATTCAATGCATACTCTTCACACGCCAGGCTGATAGAGTGACCGCTTGCGCCTGCGCGACCTGTACGACCAATACGGTGAACGTAATCTTCACAGTCATCGGGTAAATCGTCGTTAAAGACGTGCGTCACTGCCGGAATATGCAAATCACGCGCGGCAACGTCGGTGGCAACCAGAATATCCAGATCGCCACGGGTAAATTCATCACGAATACGCAGACGTTTTTTCTGCGCGACATCg                                                                                                                                              >  1:15254/1‑251 (MQ=255)                                                                                      tAGCAGGCAAATTCAATGCATACTCTTCACACGCCAGGCTGCTAGAGTGACCGCTTGCGCCTGCGCGACCTGTACGACCAATACGGTGAACGTAATCTTCACAGTCATCGGGTACATCGTAGTTAAAGACGTGCGTCACTGCCGGAATATGCAAATCACGCGCGGCAACGTCGGTGGCAACCAGCATATACAGATCGCCACGGGTAAATTCCTCAAGAATACGCAGACGTTTTTTCTGCGCGACATCGCCt                                                                                                                                           >  2:249034/1‑251 (MQ=255)                                                                                          aGGCAAATTCAATGCATACTCATCACACGCCAGGCTGATAGAGTGACCGCTTGCGCCTGCGCGACCGGTACGACCAATACGGTGAACGTAATCTTCACAGTCAACGGGTAAAGCGTAGTGAAAGACGTGCGTCACTGCCGGAATATGCAAATCACGCGCGTCAACGTCGGTGGCAACCAGAATATCCAGAGCGCCACGGGTAAATTCATCAAGAATACGCAGACGTTTTTTCTGCGCGACATCGCCTGTCa                                                                                                                                       <  2:229240/251‑1 (MQ=255)                                                                                               aaTTCAATGCATACTCTGCACACGCCAGGCTGATAGAGTGACCGCTTGCGCCTGCGCGACCTGTACGACCAATACGGTGAACGTAATCTTCACAGTCATCGGGTAAATCGTAGTTAAAGACGTGCGTCACTGCCGGAATATGCAAATCACGCGCGGCAACGTCGGTGGCAACCAGAATATCCAGATCGCCACGGGTAAATTCATCAAGAATACGCAGACGTTTTTTCTGCGCGACATCGCCTGTCAATaaa                                                                                                                                  <  2:120515/251‑1 (MQ=255)                                                                                                         aTACTCTTCACACGCCAGGCTGATAGAGTGACCGCTTGCGCCTGCGCGACCTGTACGACCAATACGGTGAACGTAATCTTCACAGTCATCGGGTAAATCGTAGTTAAAGACGGGCGTCACGGCCGGAATATGCAAATCACGCGCGGCAACGTCGGTGGCAACCAGAATATCCAGATCGCCACGGGTAAATTCATCAAGAATACGCAGACGTTTTTTCTGCGCGACATCGCCTGTCAATAAACCGACACGAt                                                                                                                        <  1:238623/251‑1 (MQ=255)                                                                                                                      gCCAGGCTGATAGAGTGACCGCTTGCGCCTGCGCGACCTGTACGACCAATACGGTGAACGTAATCTTCACAGTCATCGGGTAAATCGTAGTTAAAGACGTGCGTCCCTGCCGGAATATGCAAATCACGCGCGGCAACGTCGGTGGCAACCAGAATATCCAGATCGCCACGGGTAAATTCATCAAGAATACGCAGACGTTTTTTCTGCGCGACATCGCCTGTCAATAAACCGACACGATGACCATCtgctgc                                                                                                           <  1:163323/251‑1 (MQ=255)                                                                                                                         aGGCTGATAGAGTGACCGCTTGCGCCTTCGCGACCTGTACGACCAATACGGTGAACGTAATCTTCACAGTCATCGGGTAAATCGTAGTTAAAGACGTGCGTCACTGCCGGAATATGCAAATCACGCGCGGCAACGTCGGTGGCAACCAGAATATCCAGAGCGCCACGGGTAAATTCATCAAGAATACGCAGACGTTTTTTCTGCGCGACATCGCCTGTCAATAAACCGACACGATGACCATCTGCTGCCAg                                                                                                        <  2:100117/251‑1 (MQ=255)                                                                                                                              gATAGAGTGTCCGCTTGCGCCTGCGCGACCTGTACGACCAATACGGTGAACGTAATCTTCACAGTCATCGGGTAAATCGTAGTTAAAGACGTGCGTCACTGCCTGAATATGCAAATCACGCGCGGCAACTTCGGTGGCAACCAGAATATCCAGATCGCCACGGGTAAATTCATCAAGAATACGCAGACGTTTTTTCTGCTCGACATCGCCTGTCAATAAACCGACACGATGACCATCTGCTGCCAGGTgg                                                                                                    <  1:26175/250‑1 (MQ=255)                                                                                                                              gATAGAGTGACCGCTTGCGCCTGCGCGACCTGTACGACCAATACGGTGAACGTAATCTTCACAGTCATCGGGTAAATCGTAGTTAAAGACGTGCGTCACTGCCGGAATATGCAAATCACGCGCGGCAACGTCGGTGGCAACCAGAATATCCAGATCGCCACTGGTAAATTCATCAAGAATACGCAGACGTTTTTTCTGCGCGACATCGCCTGTCAATAAACCGACACGATGACCATCTGCTGCCAGGTGGc                                                                                                   >  1:137071/1‑251 (MQ=255)                                                                                                                                     tGACCGCTTGCGCCTGCGCGACCTGTACGACCAATACGGTGAACGTAATCTTCACAGTCATCGGGTAAATCGTAGTTAAAGACGTGCGTCACTGCCGGAATATGCAAATCACGCGCGGCAACGTCGGTGGCAACCAGAATATCCAGATCGCCACGGGTa                                                                                                                                                                                        <  1:117786/159‑1 (MQ=255)                                                                                                                                     tGACCGCTTGCGCATGCGAGACCTGTCCGCCCAATACGGTGAACGTAATCTTCACAGTCATCGGGTAAATCGTAGTTAAAGACGTGCGTCACTGCCGGAATATGCAAATCACGCGCGGCAACGTCGGTGGCAACCAGAATATCCAGATCGCCACGGGTa                                                                                                                                                                                        >  2:117786/1‑159 (MQ=255)                                                                                                                                       aCCGCTTGCGCCTGCGCGACCTGTACGACCAATACGGTGAACGTAATCTTCACAGTCATCGGGTAAATCGTAGTTAAAGACGTGCGTCACTGCCGGAATATGCAAATCACGCGCGGCAACGTCGGTGGCAACCAGAATATCCAGATCGCCACGGGTAAATTCATCAAGAATACGCAGACGTTTTTTCTGCGCGACATCGCCGGTCAATAAACCGACACGATGACCATCTGCTGCCAGGTGGCCCCAGAtct                                                                                          <  1:249034/251‑1 (MQ=255)                                                                                                                                              gcgtctgggcgACCGGTACGACCAATACGGTGAACGTAATCGTCACAGTCATCGGGTAAATCGTAGTTAAAGACGTGCGTCACTGCCGGAATATGCAAATCACGCGCGGCAACGTCGGTGGCAACCAGAATATCCAGATCGCCACGGGTAAATTCATCAATAATACGCAGACGTTTTTTCTGCGCGACATCGCCGGTCAATAAACCGACACGATGACCATCTGCTGCCAGGTGGCCCCAGATCTCTTcac                                                                                    <  2:254821/242‑1 (MQ=255)                                                                                                                                                       cgACCTGAACGACCAATACGGTGAACGTAATCTTCACAGTCATAGGGTAAATCGTAGTTAAAGACGTGCGTCACTGCCGGAATATGCAAATCACGCGCGGCAACGTCGGTGGCAACCAGAATATCCAGATCGCCACGGGTAAATTCATCAAGAATACGCAGACGTTTTTTCTGCGCGACATCGCCTGTCAATAAACCGACACGATGACCATCTGCTGCCAGGTGGCCCCAGATCTCTTCACAACGGTGttt                                                                          >  2:19071/1‑251 (MQ=255)                                                                                                                                                                            tGAACGTAATCTTCACAGTCATCGGGTAAATCGTAGTTAAAGACGTGCGTCACTGCCGGAATATGCAAATCACGCGCGGCAACGTCGGTGGCAACCAGAATATCCAGATCGACACGGGTAAATTCATCAAGAATACGCAGACGTTTTTTCTGCGCGACATCGCCTGTCAATAAACCGACACGATGACCATCTGCTGCCAGGTGGCCCCAGCTCTCTTCACAACGGTGTTTGGTGTTGGCGAAAATAATCGc                                                     >  1:162697/1‑251 (MQ=255)                                                                                                                                                                                   aaTCTTCACAGTCATCGGGTAAATCGTAGTTAAAGACGTGCGTCACTGCCGGAATATGCAAATCACGCGCGGCAACGTCGGTGGCAACCAGAATATCCAGATCGCCACGGGTAAATTCATCAAGAATACGCAGACGTTTTTTCTGCGCGACATCGCCTGTCAATAAACCGACACGATGACCATCTGCTGCCAGGTGGCCCCAGATCTCTTCACAACGGTGTTTGGTGTTGGCGAAAATAATCGCtctgtct                                              <  1:89199/251‑1 (MQ=255)                                                                                                                                                                                        tCACAGTCATCGGGTAAATCGTAGTTAAAGACGTGCGTCACTGCCGGAATATGCAAATCACGCGCGGCAACGTCGGTGGCAACCAGAATATCCAGATCGCCACGGGTAAATTCATCAAGAATACGCAGACGTTTTTTCTGCGCGACATCGCCTGTCAATAAACCGACACGATGACCATCTGCTGCCAGGTGGCCCCAGATCTCTTCACAACGGTGTTTGGTGTTGGCGAAAATAATCGCtctgtctg                                             <  1:162551/247‑1 (MQ=255)                                                                                                                                                                                         cacaGTCATCGGGTAAATCGGAGTTAAAGACGTGCGTCACTGCCGGAATATGCAAATCGCGCGCGGCAACGTCGGTGGCAACCAGAATATCCAGATCGCCACGGGTAAATTCATCAAGAATACGCAGACGTTTTTTCTGCGCGACATCGCCTGTCAATAAACCGACACGATGACCATCTGCTGCCAGGTGGCCCCAGATCTCTTCACAACGGTGTTTGGTGTTGGCGAAAATAATCGCTCTGTCTGGCCa                                         <  2:192474/250‑1 (MQ=255)                                                                                                                                                                                          acaGTCATCGGGTAAATCGTAGTTAAAGACGTGCGTCACTGCCGGAATATGCAAATCACGCGCGGCAACGTCGGTGGCAACCAGAATATCCAGATCGCCACGGGTAAATTCATCAAGAATACGCAGACGTTTTTTCTGCGCGACATCGCCTGTCAATAAACCGACACGATGACCATCTGCTGCCAGGTGGCCCCAGATCTCTTCACAACGGTGTTTGGTGTTGGCGAAAATAATCGCTCTGTCTGGCCAct                                       <  2:65504/251‑1 (MQ=255)                                                                                                                                                                                                       aaaTCGTAGTTAAAGACGTGCGTCACTTCCGGAATATGCAAATCACGCGCGGCAACGTCGTTGGCTACCAGAATATCCAGAGCGCCACGTGTAAATTCATTAAGAATACTCAGACGTTTTTTCTGCGCGACATCGCCTGTCAATAAACCGACGCGATGGCGATCGGCTGCCAGGTGGCCCCAGATCTCTTCACAACGTGGTTTGTTGTTGGCGAAAAGAATCGCTCTGTCTGGCCACTCTTCTTCGATCa                           <  1:150997/250‑1 (MQ=255)                                                                                                                                                                                                        aaTCGTAGTTAAAGAAGTGCGTCACTGCCGGAATATGCAAATCACGAGCGGCAACGTCGGTGGCAACCAGAATATCCCGATCGCCACGGGCAAATTCATCAAGAATACGCCGACGTTTTTTCTGCGCGACATCGCCTGTCAATAAACCGACACGATGACCATCTGCTGCCAGGTGGCCCCAGATCTCTTCACAACGGTGTTTGGTGTTGGCGAAAATAATCGCTCTGTCTGGCCACTCTTCTTCAATCAGc                         >  1:233015/1‑251 (MQ=255)                                                                                                                                                                                                               gTTAAAGACGTGCGTCACTGCCGGAATATGCAAATCACGCGCGGCAACGTCGGTGGCAACCAGAATATCCAGATCGCCACGGGTAAATTCATCAAGAATACGCAGACGTTTTTTCTGCGCGACATCGCCTGTCAATAAACCGACACGATGACCATCTGCTGCCAGGTGGCCCCAGATCTCTTCACAACGGTGTTTGGTGTTGGCGAAAATAATCGCTCTGTCTGGCCACTCTTCTTCGATCAGCGTTTgca                  <  1:35802/251‑1 (MQ=255)                                                                                                                                                                                                                      aCGTGCGTCACTGCCGGAATATGCAAATCACGCGCGGCAACGTCGGTGGCAACCAGAATATCCAGATCGCCACGGTTAAATTCATCAAGAATACGCAGACGTTTTTTCTGCGCGACATCGCCTGTCAATAAACCGACACGATGACCATCTGCTGCCAGGTGGCCCCAGATCTCTTCACAACGGTGTTTGGTGTGGGCGAAAATAATCGCTCTGTCGGTCCACTCTTCTTCGAGCAGCGTTTTCAGCAAACg           <  2:39425/251‑1 (MQ=255)                                                                                                                                                                                                                          gCGTCACTGCCGGAATATGCAAATCACGCGCGGCAACGTCGGTGGCAACCAGAATATCCAGATCGCCACGGGTAAATTCATCAGGAATACGCAGACGTTTTTTCTGCGCGACATCGCCTGTCAATAAACCGACACGATGACCATCTGCTGCCAGGTGGCCCCAGATCTCTTCACAACGGTGTTTGGTGTTGGCGAAAATAATCGCTCTGTCTGGCCACTCTTCTTCGATCAGCGTTTGCAGCAAACGCAt        <  1:119309/250‑1 (MQ=255)                                                                                                                                                                                                                              cACTGCCGGAATATGCAAATCACGCGCGGCAACGTCGGTGGCAACCAGAATATCCAGATCGCCACGGGTAAATTCATCAAGAATACGCAGACGTTTTTTCTGCGCGACATCGCCTGTCAATAAACCGACACGATGACCATCTGCTGCCAGGTGGCCCCAGATCTCTTCACAACGGTGTTTGGTGTTGGCGAAGATAATCGCTCTGTCTGGCCACTCTTCTTCGATCAGCGTTTGCAGCAAACGCAtttttt   >  1:52658/1‑251 (MQ=255)                                                                                                                                                                                                                               aCTGCCGGAATATGCAACTCACGCGCGGCAACGTCGGTGGCAACAAGAATATCCAGATCGACCCGGGAAAATTAATCAAGAATACGCAGACGTTTTTTCTGCGCGACATCGCCTGACAATAAACCGACCCGATGACCATCTGCTGCCACGCGGCCCCAGATCTCTTCCCAACGGTGTTTGGTGTTGGCGAAAATAATCGCTCTGTCTTGACCCTCTTCTTCGATCAGCGATTGCAGCAAACGCATTTTttc  >  1:213889/1‑251 (MQ=255)                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                         AGGCGCAGCGGTTTTGGCAGATCGGTCATCAATGCGTCCGGATTGTATTTGCTTACCGGAATTGAGTGACCAATATAGGTCTCAATAGCAGGCAAATTCAATGCATACTCTTCACACGCCAGGCTGATAGAGTGACCGCTTGCGCCTGCGCGACCTGTACGACCAATACGGTGAACGTAATCTTCACAGTCATCGGGTAAATCGTAGTTAAAGACGTGCGTCACTGCCGGAATATGCAAACCACGCGCGGCAACGTCGGTGGCAACCAGAATATCCAGATCGCCACGGGTAAATTCATCAAGAATACGCAGACGTTTTTTCTGCGCGACATCGCCTGTCAATAAACCGACACGATGACCATCTGCTGCCAGGTGGCCCCAGATCTCTTCACAACGGTGTTTGGTGTTGGCGAAAATAATCGCTCTGTCTGGCCACTCTTCTTCGATCAGCGTTTGCAGCAAACGCATTTTTTC  >  NC_000913/3964435‑3964907

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑