Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,667,950	G→A	Y413Y (TAC→TAT)	ccp ←	cytochrome c peroxidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,667,950	0	G	A	100.0%	47.1 / NA	15	Y413Y (TAC→TAT)	ccp	cytochrome c peroxidase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (9/6);  total (9/6)

AAACATTTAACATGATAATATTAAAAACAGACTTTATTATCGTAATAAAAATCGCCCAAACATTGCTGTTCGGGCGATCAAATTAATTATTGTTTATCCTGCATATACGGCGTGTACACCCCGTTCAGACTGTGCAGGAAAGCTACGATATCATCCACATCCTCCTGCGGCAGCTCTTTGCCTACCTGATAGCGCAGCATCAGTTTCACCGCCCCGTCCAGCGTCGGCACGTCACCGCGATGGAAGTACGGTGCCGTTAAAGCAACGTTACGTAAACCGGGTACTTTCTGACGCAATTTATCACGCTCTTCTTTAGTCACATTCATACGACCAATATCCGCCGCCGTAATTTCCCCAAAGTTAAAGTCTTTTTTCAGCCCCAACGGTTCAAAGGAACGTCCGCCGAGAATAATACCACCATGACAAGTTGCACATTTATTATCTTTAAATAATTGATAGCCTTTTTTCTGT  >  NC_000913/3667705‑3668175                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                  aaaCATTTAACATGATAATATTAAAAACAGACTTTATTATCATAATAAAAATCGCCCAAACATTGCTGTTCGGGCGATCAAATTAATTATTGTTTATCCTGCATATACGGCGTGTACACCCCGTTCAGACTGTGCAGGAAAGCTACGATATCATCCACATCCTCCTGCGGCAGCTCTTTGCCTACCTGATAGCGCAGCATCAGGTTCACCGCCCCGTCCCGCGTCGGCCCGTCCCCGCGATGGGAATACgg                                                                                                                                                                                                                              >  2:85390/1‑251 (MQ=255)                       aaaaaCAGACTTTATTATCGTAATAAAAATCGCCCAAACATTGCTGTTCGGGCGATCAAATTAATTATTGTTTATCCTGCATATACGGCGTGGACACCCCGTTCAGACTGTGCAGGAAAGCTACGATATCATCCACATCCTCCTGCGGCAGCTCTTTGCCTACCTGATAGCGCAGCATCAGTTTCACCGCCCCGACCAGCGTCGGCACGGCACCGCGATGGAAATACGGGGCCGTTAAAGCAACGTTACgg                                                                                                                                                                                                        >  1:159733/1‑250 (MQ=255)                         aaaCAGACTTTATTATCGTAATAAAAATCGCCCAAACATTGCTGTCCGGGCGCTCAAATTAATTATTGTTTATCCTGCATATACGGCGTGTACACCCCGTTCAGACTGTGCAGGAAAGCTACGATATCATCCACATCCTCCTGCGGCCGCTCTTTGCCTACCTGATAGCGCAGCATCAGCTTCACCGCCCCGTCCAGCGTCGGCCCGTCACCGCGCTGGAAATACGGTGCCGTTAAAGCAACGTTACGTa                                                                                                                                                                                                       >  2:17715/1‑250 (MQ=255)                                                          aaaCATTGCTGTTCGGGCGATCAAATTAATTATTGTTTATCCTGCATATACGGCGTGTACACCCCGTTCAGACTGTGCAGGAAAGCTACGATATCATCCACATCCTCCTGCGGCAGCTCTTTGCCTACCTGATAGCGCCGCATCAGTTTCACCGCCCCGTCCAGCGTCGGCACGTCACCGCGATGGAAATACGGTGCCGTTAAAGCAACGTTACGTAAACCGGGTACTTTCTGACGCAATTTATCACGctc                                                                                                                                                                     >  1:8660/1‑251 (MQ=255)                                                                                                             ggCGTGTACACCCCGTTCAGACTGTGCAGGAAAGCTACGATATCATCCACATCCTCCTGCGGCAGCTCTTTGCCTACCTGATAGCGCAGCATCAGTTTCACCGCCCCGTCCAGCGTCGGCACGTCACCGCGATGGAAATACGGTGCCGTTAAAGCAACGTTACGTAAACCGGGTACTTTCTGACGCAATTTATCACGCTCTTCTTTAGTCACAtt                                                                                                                                                      >  1:209347/1‑215 (MQ=255)                                                                                                             ggCGTGTACACCCCGTTCAGACTGTGCAGGAAAGCTACGATATCACCCACACCCTCCGGCGGCAGCTCTTTGCCTACCTGATAGCGCAGCATCAGTTTCACCGCCCCGTCCAGCGTCGGCACGTCACCGCGATGGAAATACGGTGCCGTTAAAGCAACGTTACGTAAACCGGGTACTTTCTGACGCAATTTATCACGCTCTTCTTTAGTCACAtt                                                                                                                                                      <  2:209347/215‑1 (MQ=255)                                                                                                               cGTGTACACCCCGTTCAGACTGTGCAGGAAAGCTACGATATCATCCACATCCTCCTGCGGCAGCTCTTTGCCTACCTGATAGCGCAGCATCAGTTTCACCGCCCCGTCCAGCGTCGGCACGTCACCGCGATGGAAATACGGTGCCGTTAAAGCAACGTTACGTAAACCGGGTACTTTCTGACGCAATTTATCACGCTCTTCTTTAGTCACATTCATACGACCAATATCCGCCGCCGTAATTTCACCAAAGt                                                                                                                >  2:70984/1‑251 (MQ=255)                                                                                                                           gTTCAGACTGTGCAGGAAAGCTACGATATCATCCCCACCCTCCGGCGGCAGCTCTTTGCCTACCTGATAGCGCAGCATCAGTTTCCCCGCCCCGTCCAGCGTCGGCACGTCACCGCGATGGAAATACGGTGCCGTTAAAGCAACGTTACGTAAACCGGGTACTTTCTGACGCAATTTATCACGCTCTTCTTTAGTCACATTCATACGACCAATATCCGCCGCCGTAATTTCCCCAAAGTTAAAGTCttttt                                                                                                    <  1:102061/251‑1 (MQ=255)                                                                                                                                           aaaGCTACGATATCATCCACATCCTCCTGCGGCAGCTCTTTGCCTACCTGATAGCGCAGCATCAGTTTCACCGCCCCGTCCAGCGTCGGCACGTCACCGCGATGGAAATACGGTGCCGTTAAAGCAACGTTACGTAAACCGGGTACTTTCTGACGCAATTTATCACGCTCTTCTTTAGTCACATTCATACGACCAATATCCGCCGCCGTAATTTCCCCAAAGTTAAAGTCTTTTTTCAGCCCCAACGGTTc                                                                                    >  2:50700/1‑251 (MQ=255)                                                                                                                                                                        cGGCAGCTCTTTGCCTACCTGATAGCGCAGCATCAGTTTCACCGCCCCGTCCAGCGTCGGCACGTCACCGCGATGGAAATACGGTGCCATTAAAGCAACGTTACGTAAACCGGGTACTTTCTGACGCAATTTATCACGCTCTTCTTTAGTCACATTCATACGACCAATATCCGCCGCCGTAATTTCCCCACAGTTAAAGTCTTTTTTCAGCCCCAACGGTTCAAAGGACCGTCCGCCGAGAATCATaccac                                                       >  2:263567/1‑251 (MQ=255)                                                                                                                                                                         ggccgCTCTTTGCCTACCTGATAGCGCAGCATCAGTTTCACCGCCCCGTCCAGCGTCGGCACGTCCCCGCGATGGAAATACGTTGCCGTTAAAGCAACGTTACGTAAACCGTGTACTTTCTGACGCAATTTATCACGCTCTTCTTTAGTCACATTCATACGCCCAATATCCGCCGCCGTAATTTCCCCAAAGTTAAAGTCTTTTTTCAGCCCCAACGGTTCAAAGGAACGTCCGCCGAGAATAATaccacc                                                      <  1:85390/247‑1 (MQ=255)                                                                                                                                                                                                  ccggcaTCAGTTTCACCGCCCCGTCCAGCGTCGGCACGTCACCGCGATGGAAATACGGTGCCGTTAAAGCAACGTTACGTAAACCGGTTACTTTCTGACGCAATTTATCACGCTCTTCTTTAGTCACATTCATACGACCAATATCCGCCGCCGTAATTTCCCCAAAGTGAAAGGCTTTTTTCAGCCCCAACGGTTCAAAGGAACGTCCGCCGAGAATAATACCACCATGACAAGTTGCACATTTATTATCt                             <  1:170038/248‑1 (MQ=255)                                                                                                                                                                                                                           cAGCGTCGGCACGTCACCGCGATGGAAATACGGTGCCGTTAAAGCAACGTTACGTAAACCGGGTACTTTCTGACGCAATTTATCACGCTCTTCTTTAGTCACATTCATACGACCAATATCCGCCGCCGTAATTTCCCCAAAGTTAAAGTCTTTTTTCAGCCCCAACGGTTCAAAGGAACGTCCGCCGAGAATAATACCACCATGACAAGTTGCACATTTATTATCTTTAAATAATTGATAGCCttttt       <  1:70388/248‑1 (MQ=255)                                                                                                                                                                                                                           cAGCGTCGGCACGTCACCGCGATGGAAATACGGTGCCGTTAAAGCAACGTTACGTAAACCGGGTACTTTCTGACGCAATTTATCACGCTCTTCTTTAGTCACATTCATACGACCAATATCCGCCGCCGTAATTTCCCCAAAGTTAAAGTCTTTTTTCAGCCCCAACGGTTCAAAGGAACGTCCGCCGAGAATAATACCACCATGACAAGTTGCACATTTATTATCTTTAAATAATTGATAGCCttttt       >  2:70388/1‑248 (MQ=255)                                                                                                                                                                                                                             gCGTCGGCACGTCACCGCGATGGAAATACGGTGCCGTTAAAGCAACGTTACGTAAACCGGGTACTTTCTGACGCAATTTATCACGCTCTTCTTTAGTCACATTCATACGACCAATATCCGCCGCCGTAATTTCCCCAAAGTTAAAGTCTTTTTTCAGCCCCAACGGTTCAAAGGAACGTCCGCCGAGAATAATACCACCATGACAAGTTGCACATTTATTATCTTTAAATAATTGATAGCCTTTTTTCTGt  <  1:17715/251‑1 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                  AAACATTTAACATGATAATATTAAAAACAGACTTTATTATCGTAATAAAAATCGCCCAAACATTGCTGTTCGGGCGATCAAATTAATTATTGTTTATCCTGCATATACGGCGTGTACACCCCGTTCAGACTGTGCAGGAAAGCTACGATATCATCCACATCCTCCTGCGGCAGCTCTTTGCCTACCTGATAGCGCAGCATCAGTTTCACCGCCCCGTCCAGCGTCGGCACGTCACCGCGATGGAAGTACGGTGCCGTTAAAGCAACGTTACGTAAACCGGGTACTTTCTGACGCAATTTATCACGCTCTTCTTTAGTCACATTCATACGACCAATATCCGCCGCCGTAATTTCCCCAAAGTTAAAGTCTTTTTTCAGCCCCAACGGTTCAAAGGAACGTCCGCCGAGAATAATACCACCATGACAAGTTGCACATTTATTATCTTTAAATAATTGATAGCCTTTTTTCTGT  >  NC_000913/3667705‑3668175

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑