Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,329,743	G→T	D261Y (GAT→TAT)	dacB →	peptidoglycan DD‑endopeptidase DacB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,329,743	0	G	T	100.0%	92.0 / NA	28	D261Y (GAT→TAT)	dacB	peptidoglycan DD‑endopeptidase DacB
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (17/11);  total (17/11)

AGCCTGGTGATATGGCTTTTATACGCGTGGCATCTTATTACCCCGTTACGATGTTCAGCCAGGTACGCACCCTCCCCCGTGGTTCTGCCGAAGCGCAATACTGCGAACTGGATGTGGTGCCAGGCGACCTGAACCGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAAGATGAGTTAAAACAGGCGGGTATCACCTGGAGCGGAACACTGCTGCGCCAGACTCAGGTTAACGAACCTGGAACGGTAGTTGCCAGTAAACAGTCGGCCCCGCTGCACGATCTGCTTAAGATTATGCT‑GAAAAAGTCGGACAACATGATCGCCGATACGGTTTTCCGCATGATAGGCCATGCGCGCTTCAATGTGCCTGGAACATGGCGGGCCGGGTCGGACGCCGTGCGTCAGATCCT  >  NC_000913/3329510‑3329981                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                aGCCTGGTGATATGGCTTTTATACGCGTGGCATCTTATTACCCCGTTACGATGTTCAGCCAGGTACGCACCCTCCCCCGTGGTTCTGCCGAAGCGCAATACTGCGAACTGGATGTGGTGCCAGGCGACCTGAACCGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCg                                                                                                                                                                                                                                <  1:119454/251‑1 (MQ=255) aGCCTGGGGATATGGCTTTTATACGCTTGGCGTCTTATGCCCCCGTTACGAGGTTCAGCCAGGTACGCACCCTCCCCCGTGGTGCGGCCGAAGCGCAAGACTGCGAACTGGATGTGGTGCCGGGCGTCCTGAACCGCTTTACGCTGACGGGATGCCTGCCACAACTCTCTGAGCCGCTTCCTTTGGCTTTTGCCGTGCAGGAGGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCg                                                                                                                                                                                                                                <  1:149933/251‑1 (MQ=255)         gATATGGCTTTTATACGCGTGGCATCTTATTACCCCGTTACGATGTTCAGCCAGGTACGCACCCTCCCCCGTGGTTCTGCCGAAGCGCAATACTGCGAACTGGATGTGGTGCCAGGCGACCTGAACCGCTT‑AAGATGACGGGGAGCCTGCCACAACGTTTTGAGCCGCTCCCGGTGGCTTTTGCCGTGCAGGATGGAGCCCGCTATGCCGGTGCAAATCTGCAATATGAGTTCAAAAAGGCGGGTATcctc                                                                                                                                                                                                                       >  2:93945/1‑248 (MQ=255)                  tttATACGCGTGGCATCTTATTACCCCGTTACGCTGTTCAGCCAGGTACGCACCCTCCCCCGTGGTTCTGCCGAAGCGCAATACTGCGAACTGGATGTGGTGCCAGGCGACCTGAACCGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCACCTTGAGCgg                                                                                                                                                                                                               >  2:260428/1‑251 (MQ=255)                                   ttattaCCCCGTTACGATGTTAAGCCAGGTCCGCACCCTCCCCCGTGGTTCTGCCGAAGCGCAATACTGCGAAATGGATGTGGTGCCAGGCGACCTGAACCGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTCTGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGAATCACCTGGAGCGGGACACTGCTGCGcccg                                                                                                                                                                                               >  2:233461/1‑248 (MQ=255)                                            cGTTACGATGTTCAGCCAGGTACGCACCCTCCCCCGTGGTTCTGCCGAAGCGCAATACTGCGAACTGGATGTGGTGCCAGGCGACCTGAACCGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGGGTCCCCTGGAGCGGGAAACTGCTGCGCCAGAATCAGGtt                                                                                                                                                                                      >  1:261308/1‑250 (MQ=255)                                            cGTTACGAGGTTCACCCAGGGACGCCCCCCCCCCCGTGGTTCTGGCGGAGCGCAATACTGCGAACTGGATGTGGTGCCAGGCGACCTTAACCTCTTTACGCTGAGGGGTTGCCTGCCCCAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGATGCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAATAGGCTGGTATCACCTTGAGCGGAGCGCTGCTGCGCCAGTCTTAGGTTa                                                                                                                                                                                     <  2:29060/251‑1 (MQ=255)                                                   aTGTTCAGCCAGGTACGCACCCTCCCCCGTGGTTCTGCCGAAGCGCAATACTGCGAACTGGATGTGGTGCCAGGCGACCTGAACCGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCACCTGGAGAGGCACACTGCTGCGCCAGAATCCGGGTAACGaaac                                                                                                                                                                              >  2:53808/1‑249 (MQ=255)                                                      ttCAGCCAGGTACGCACCCTCCCCCGTGGTTCTGCCGAAGCGCAATACTGCGAACTGGATGTGGTGCCAGGCGACCTGAACCGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCACCTGGAGCGGAACACTGCTGCGCCAGACTCAGGTTAACGAACCTgg                                                                                                                                                                           >  1:14903/1‑251 (MQ=255)                                                       tccgCCAGGTCCGCACCCTCCCCCGGGGTTCGGCCGAAGCGCAATACTGCGATCTGGATTTGGTGCCAGGCGACCTGAACCGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTGGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCACCTGGAGCGGAACACTGCTGCGCCAGACTCAGTTTAACGAACCTgg                                                                                                                                                                           <  2:62737/247‑1 (MQ=255)                                                                               gTGGTTCTGCCGAAGCGCAATACTGCGAACTGGATGTGGTGCCAGGCGACCTGAACCGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCACCTGGAGCGGAACACTGCTGCGCCAGAATCAGGGTAACGAACCTTGAACGGGAGTTGCACGTAAACCGTCg                                                                                                                                                  >  2:91035/1‑251 (MQ=255)                                                                                              cgcAATACTGCGAACTGGATGTGGTGCCAGGCGACCTGAACCGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCACCTGGAGCGGAACACTGCTGCGCCAGACTCAGGTTAACGAACCTGGAACGGTAGTTGCCAGTAAACAGTCGGCCCCGCTGCACGAt                                                                                                                                   >  1:21482/1‑251 (MQ=255)                                                                                                    aCTGCGAACTGGATGTGGTGCCAGGCGACCTGAACCGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCACCTGGAGCGGAACACTGCTGCGCCAGACTCAGGTTAACGAACCTGGAACGGTAGTTGCCAGTAAAAAGTCGGCCCCGCTGCACGATCTGCtt                                                                                                                             >  1:36946/1‑251 (MQ=255)                                                                                                         gAACTGGATGTGGTGCCAGGCGACCTGAACCGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCACCTGGAGCGGAACACTGCTGCGCCAGACTCAGGTTAACGAACCTGGAACGGTAGTTGCCAGTAAAAAGGCGGCCCCGCCGCACGATCTGCTTa                                                                                                                            >  1:37456/1‑247 (MQ=255)                                                                                                              ggATGTGGTGCCAGGCGACCTGAACCGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCACCTGGAGCGGGACACTGCTGCGCCAGACTCAGGTTAACGAACCTGGAACGGTAGTTGCCAGTAAACAGTCCGCCCCGCTGCACGATCTGCTTAAGATTATGc                                                                                                                   >  1:134578/1‑251 (MQ=255)                                                                                                                          aGGCGACCTGAACCGCTTTACGCTGCCGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCACCTGGAGCGGAACACTGCTGCGCCAGACTCAGGTTAACGAACCTGGAACGGGAGTTGCCAGTAAACAGTCGGCCCCGCTGCCCGATCTGCTTAAGATTATGCTTGaaaagacgg                                                                                                       >  2:238605/1‑246 (MQ=255)                                                                                                                                  tGAACCGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGGATCACCTGGAGCGGAACACTGCTGCGCCAGACTCAGGTTAACGAACCTGGAACGGGAGTTGCCAGTAAACAGTCGGCCCCGCTGCACGATATGCTTAAGATTATGCT‑GAAAAAGTCGGaccaca                                                                                                >  1:217217/1‑249 (MQ=255)                                                                                                                                   gAACCGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCACCTGGAGCGGAACACTGCTGCGCCAGACTCAGGTTAACGAACCTGGAACGGTAGTTGCCAGTAAACAGTCGGCCCCGCTGCACGATCTGCTTAAGATTATGCT‑GAAAAAGTCGGACAACATg                                                                                              <  1:126079/250‑1 (MQ=255)                                                                                                                                     aCCGCTTTCCGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCACCTGGAGCGGAACACTGCTGCGCCAGACTCAGGTTAACGAACCTGGAACGGTAGTTGCCAGTAAACAGTCGGCCCCGCTGCACGATCTGCTTAAGATTATGCT‑GAAAAAGTCGGACAACATGATc                                                                                           <  1:212375/251‑1 (MQ=255)                                                                                                                                                  gACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCACCTGGAGCGGAACACTGCTGCGCCCGACTCAGGGTAACGAACCTGGGACGGGAGATGCCAGTAAACAGTCGGCCCCGCTGCACGATCTGCTTATGATTATGCT‑GAAAAAATCGGGCAACATGATCGCCGGTACgggtt                                                                              >  2:55181/1‑248 (MQ=255)                                                                                                                                                              gccACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCACCTGGAGCGGAACACTGCTGCGCCAGACTCAGGTTAACGAACCTGGAACGGTAGTTGCCAGTAAACAGTCGGCCCCGCTGCACGATCTGCTTAAGATTATGCT‑GAAAAAGTCGGACAACATGATCGCCGATACGGTTTTCCGCATGATa                                                                   <  2:3828/250‑1 (MQ=255)                                                                                                                                                               ccACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCACCTGGAGCGGAACACTGCTGCGCCAGACTCAGGTTAACGAACCTGGAACGGTAGTTGCCAGTAAAAAGTCGGCCCCGCTGCACGATCTGCTTAAGATTATGCT‑TAAAAAATCGGACAACATTATCGCCGATACGGTTTTCCGCATGATAgg                                                                 >  1:102876/1‑251 (MQ=255)                                                                                                                                                                           gAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCCGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTAACACCTGGAGCGGGACACTGCTGCGCCAGACTCAGGGTAACGCACCTGGGACGGGAGTTGCCCGTAAACCCTCCGCCCCGCTGCACGATCTGCTTTAGCTTATGCT‑GAAAAAGTCGGACAACATGATCGCCGATACGGTTTTCCGCATGAAAGGCCATGCGCGCtt                                                     >  2:234608/1‑251 (MQ=255)                                                                                                                                                                               cGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCACCTGGAGCGGAACACTGCTGCGCCAGACTCAGGTTAACGAACCTGGAACGGTAGTTGCCAGTAAACAGTCGGCCCCGCTGCACGATCTGCTTAAGATTATGCT‑GAAAAAGTCGGACAACATGATCGCCGATACGGTTTTCCGCATGATAGGCCATGCGCGCTTCAAt                                                 <  1:233461/251‑1 (MQ=255)                                                                                                                                                                                                       aGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCACCTGGAGCGGAACACTGCTGCGCCAGACTCAGGTTAACGAACCTGGAACGGTAGTTGCCAGTAAACAGTCGGCCCCGCTGCACGATCTGCTTAAGATTATGCT‑GAAAAAGTCGGACAACATGATCGCCGATACGGTTTTCCGCATGATAGGCCATGCGCGCTTCAATGTGCCTGGAACATGGCGGGCCggg                         <  1:2915/251‑1 (MQ=255)                                                                                                                                                                                                            ggAGCCAGCTATGCCGGTGCAATTCTGAAATATGAGTGAAAACAGGCGGGTATCACCTGGAGCGGAACACTGCTGCGCCAGACTCAGGTTAACGAACCTGGAACGGTAGTTGCCAGTAAACAGTCGGCCCCGCTGCACGATCTGCTTAAGATTATGCT‑GAAAAAGTCGGACAACATGATCGCCGATACGGTTTTCCGCATGATAGGCCATGCGCGCTTCAATGTGCCTGGAACATGGCGGGCCGGGTCGGa                    <  2:14903/251‑1 (MQ=255)                                                                                                                                                                                                                             tGCAATTCTGAAATATGAGTTAAAACAGGCGGGCATCCCCTGGAGCGGAACCCTGCTGCGCAAGAATCAGGGTAACGAACCTGGCACGGTAGTTGCCAGTAAAAAGTCCGCCCCCCTGCCCGATCTGCTTAAGATTATGCC‑GAAAAAGTCGGCCAACATGATCGCCGATACGGTTTTCCGCATGATAGGCCATGCGCGCTTCAATGTGCCTGGCACATGGCGGGcccgggcgggcgcgccgcggcagaccc   >  2:149819/1‑226 (MQ=255)                                                                                                                                                                                                                              gCAATTCTGAAATATGAGTTAAAACAGGCGGGTATCACCTGGAGCGGAACACTGCTGCGCCAGACTCAGGTTAACGAACCTGGAACGGTAGTTGCCAGTAAACAGTCGGCCCCGCTGCACGATCTGCTTAAGATTATGCT‑GAAAAAGTCGGACAACATGATCGCCGATACGGTTTTCCGCATGATAGGCCATGCGCGCTTCAATGTGCCTGGAACATGGCGGGCCGGGTCGGACGCCGTGCGTCAGATCCt  <  1:260428/251‑1 (MQ=255)                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                AGCCTGGTGATATGGCTTTTATACGCGTGGCATCTTATTACCCCGTTACGATGTTCAGCCAGGTACGCACCCTCCCCCGTGGTTCTGCCGAAGCGCAATACTGCGAACTGGATGTGGTGCCAGGCGACCTGAACCGCTTTACGCTGACGGGATGCCTGCCACAACGTTCTGAGCCGCTCCCGTTGGCTTTTGCCGTGCAGGATGGAGCCAGCTATGCCGGTGCAATTCTGAAAGATGAGTTAAAACAGGCGGGTATCACCTGGAGCGGAACACTGCTGCGCCAGACTCAGGTTAACGAACCTGGAACGGTAGTTGCCAGTAAACAGTCGGCCCCGCTGCACGATCTGCTTAAGATTATGCT‑GAAAAAGTCGGACAACATGATCGCCGATACGGTTTTCCGCATGATAGGCCATGCGCGCTTCAATGTGCCTGGAACATGGCGGGCCGGGTCGGACGCCGTGCGTCAGATCCT  >  NC_000913/3329510‑3329981

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑