Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,042,536	T→C	S205G (AGT→GGT)	yqfA ←	hemolysin‑III family protein YqfA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,042,536	0	T	C	100.0%	55.8 / NA	21	S205G (AGT→GGT)	yqfA	hemolysin‑III family protein YqfA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (13/8);  total (13/8)

CTTTATCGCATCAGGCATTTATCGCCTGATGCGACGCTGGCGCGTCTTATCATGTCTGGGATTTGATGCCTTTATCACAACAGGGACGAGGCCCGTAGATCGGATAAGGCGTTC‑ACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACAAAGGCATACCCATTACGCCTGCCCAATATACAAATAGATCGCCAGAAAGTGGC‑ACACACTACCGCCGAGCACGAAGCCGTGCCAGATGGCATGGTTGTATGGAATGCGTTTGCAGACGTAGAAAATCACCCCGAGCGAATAAACCACGCCGCCTACCGCCAGTAAGGTAACGCTGCCCGCCGCGAGCTTAACTGCCATTTCATAAATTACCACCAGCGACAGCCAGCCCATCGCCAGATAGGTCACCAGAGATAAAATTTTGAATCGGTGCGCGATGGTCAGTTTAA  >  NC_000913/3042298‑3042763                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                    ctTTATCGCATCAGGCATTTACCGCCTGATGCGACGCTGGCGCGTCTTATCATGTCTGGGATTTGATGCCTTTATCCCAACAGGGACGAGGCCCGTAGATCGGATAAGGCGTTC‑ACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACAAAGGCAGACCCATTACGCCTGCCCAATATACAAATAGATCGCCAGAAAGTGGC‑ACACACCACCGCCGAGc                                                                                                                                                                                                                           <  2:152716/249‑1 (MQ=255)   ttATCGCATCAGGCATTTATCGCCTGATGCGACGCTGGCGCGTCTTATCATGTCTGGGATTTGATGCCTTTATCACAACAGGGACGAGGCCCGTAGATCGGATAAGGCGTTC‑ACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACAAAGGCATACCCATTACGCCTGCCCAATATACAAATAGATCGCCAGAAAGTGGC‑ACACACCACCGCCGAGCa                                                                                                                                                                                                                          <  2:95649/248‑1 (MQ=255)        gCATCAGGAATTTATCGCCTGATGCGACGCTGGCGCGTCTTATCCTGTCTGGGATTTGATGCCTTTATCACAACAGGGACGAGGCCCGTAGATCGGATAAGGCGTTC‑ACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACAAAGGCATACCCATTACGCCTGCCCAATATACAAATAGATCGCCAGAAAGTGGC‑ACACACCACCGCCGAGCCCGCAGCCg                                                                                                                                                                                                                  >  2:214471/1‑251 (MQ=255)         cATCAGGCATTTATCGCCTGATGCGACGCTGGCGCGTCTTATCATGTCTGGGATTTGATGCCTTTATCACAACAGGGACGAGGCCCGTAGATCGGATAAGGCGTTC‑ACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACAAAGGCATACCCATTACGCCTGCCCAATATACAAATAGATCGCCAGAAAGTGGC‑ACACACCACCGCCGAGCACGAAGCCGt                                                                                                                                                                                                                 <  1:54817/251‑1 (MQ=255)                        gCCTGATGCGACGCTGGCGCGTCTTATCATGTCTGGGATTTGATGCCTTTATCACAACAGGGACGAGGCCCGTAGATCGGATAAGGCGTTC‑ACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACAAAGGCATACCCATTACGCCTGCCCAATATACAAATAGATCGCCAGAAAGTGGC‑ACACACCACCGCCGAGCACGAAGCCGTGCCAGATGGCATggg                                                                                                                                                                                                  >  2:116869/1‑250 (MQ=255)                                ggACGCTGGCGCGTCTTATCATGTCTGGGATTTGATGCCTTTATCACAACAGGGACGAGGCCCGTAGATCGGATAAGGCGTTC‑ACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACAAAGGCATACCCATTACGCCTGCCCAATATACAAATAGATCGCCAGAAAGTGGC‑ACACACCACCGCCGAGCACGAAGCCGTTCCAGATGGCATGGTTGTATGGa                                                                                                                                                                                          <  1:79014/250‑1 (MQ=255)                                            gTCTTATCATGTCTGGGATTTGATGCCTTTATCACAACAGGGACGAGGCCCGTAGATCGGATAAGGCGTTC‑ACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACAAAGGCATACCCATTACGCCTGCCCAATATACAAATAGATCGCCAGAAAGTGGC‑ACACACCACCGCCGAGCACGAAGCCGTGCCAGATGGCATGGTTGTATGGAATGCGTTTGc                                                                                                                                                                                >  1:108424/1‑249 (MQ=255)                                                tATCATGTCTGGGATTTGATGCCTTTATCACAACAGGGACGAGGCCCGTAGATCGGATAAGGCGTTC‑ACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACAAAGGCATACCCATTACGCCTGCCCAATATACAAATAGATCGCCAGAAAGTGGC‑ACACACCACCGCCGAGCACGAAGCCGTGCCAGATGGCATGGTTGTATGGAATGCGTTTGCAGACg                                                                                                                                                                           <  1:29575/250‑1 (MQ=255)                                                      gTCTGGGATTTGATGCCTTTATCACAACAGGGACGAGGCCCGTAGATCGGATAAGGCGTTC‑ACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACAAAGGCATACCCATTCCGCCTGCCCAATATACAAATAGATCGCCAGAAAGTGGC‑ACACACCACCGCCGAGCACGAAGCCGTGCCAGATGGCATGGTTGTATG‑AATGCGGTTGCAGACGTAGACAAt                                                                                                                                                                   >  2:6262/1‑251 (MQ=255)                                                                   tGCCTTTATCACAACAGGGACGAGGCCCGTAGATCGGATAAGGCGTTC‑ACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACAAAGGCATACCCATTACGCCTGCCCAATATACAAATAGATCGCCAGAAAGTGGC‑ACACACCACCGCCGAGCACGAAGCCGTGCCAGATGGCATGGTTGTATGGAATGCGTTTGCAGACGTAGAAAATCACCCCGAGCg                                                                                                                                                        >  2:107916/1‑250 (MQ=255)                                                                                          ggCCCGTAGATCGGAAAAGGCGTTC‑ACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACAAAGTCATACCCATTACGCCTGCCCAATATACAAATAGATCGCCAGAAAGTGGC‑ACACACCACCGCCGAGCACGAAGCCGTGCCAGATGGCATGGTTGTATGGAATGCGTTTGCAGACGTAGAAAATCACCCCGAGCGAATAAACCACGCCGCCTACCGCCa                                                                                                                                <  2:86564/251‑1 (MQ=255)                                                                                           gCCCGTAGATCGGATAAGG‑GTTCCACGCCGAATCCGGCAGTTGTGCTCCGAGGCCTGATGCGACGCTGGCGCGTCTTAGCAGGCCTACAAAGGCATACCCATTACGCCTGCCCAATATACAAATAGATCGCCAGAAAGTGGC‑ACACACCACCGCCGAGCACGAAGCCGTGCCAGATGGCATGGTTGTATGGAATGCGTTTGCAGACGTAGAAAATCACCCCGAGCGAATAAACCACGCCGCCTACCGCCAg                                                                                                                               <  2:258067/251‑1 (MQ=255)                                                                                               gTAGATCGGATAAGGCGTTC‑ACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACAAAGGCATACCCATTACGCCTGCCCAATATACAAATAGATCGCCAGAAAGTGGC‑ACACACCACCGCCGAGCACGAAGCCGTGCCAGATGGCATGGTTGTATGGAATGCGTTTGCAGACGTAGAAAATCACCCCGAGCGAATAAACCACGCCGCCTACCGCCAGTa                                                                                                                             >  1:187317/1‑249 (MQ=255)                                                                                                                                                         gccgcgggCGCGTTTTATCGGGCCTAAAAGGGCATACCCATTACGCCTGCCCAATATAAAAAAAGACCGCCA‑AAAGGGGCCACCCACCCCCGCCGAGCACGAAGCCGTGCCAGATGGCAGGGTTGTATGGAATGCGTTTGAAGACGTAAAAAATCCCCCCGAGCAAAAAACCCCCGCCGCCTACCGCCAGTAAGGTAACGCTGCCCGCCGCGAGCTTAACTGCCATTTCATAAATTACCACCAGCGAcagc                                                                  <  1:26936/245‑1 (MQ=255)                                                                                                                                                         gACGCTGGCGCGTCTTATCAGGCCTACAAAGGCATACCCATTACGCCTGCCCAATATACAAATAGATCGCCAGAAAGTGGC‑ACACACCACCGCCGAGCACGAAGCCGTGCCAGATGGCATGGTTGTATGGAATGCGTTTGCAGACGTAGAAAATCACCCCGAGCGAATAAACCACGCCGCCTACCGCCAGTAAGGTAACGCTGCCCGCCGCGAGCTTAACTTCCATTTCATAAATTACCACCAGCGAcagc                                                                  >  1:61069/1‑251 (MQ=255)                                                                                                                                                          aCGCTGGCGCGTCTTATCAGGCCTACAAAGGCATACCCATTACGCCTGCCCAATATACAAATAGATCGCCAGAAAGTGGC‑ACACACCACCGCCGAGCACGAAGCCGTGCCAGATGGCATGGTTGTATGGAATGCGTTTGCAGACGTAGAAAATCACCCCGAGCGAATAAACCACGCCGCCTACCGCCAGTAAGGTAACGCTGCCCGCCGCGAGCTTAACTGCCATTTCATAAATTACCACCAGCGAcagcc                                                                 >  1:134214/1‑251 (MQ=255)                                                                                                                                                             cTGGCGCGTCTTATAAGGCCTACAAAGGCATACCCATTACGCCTGCCCAATATACAAATAGATCGCCAGAAAGTGGC‑ACACACCACCGCCGAGCACGAAGCCGTGCCAGATGGCATGGTTGTATGGAATGCGTTTGCAGACGTAGAAAATCACCCCGAGCGAATAAACCACGCCGCCTACCGCCAGTAAGGTAACGCTGCCCGCCGCGCGCTTAACTGCCATTTCATAAATTACCACCAGCGAcagccagc                                                              >  1:79231/1‑251 (MQ=255)                                                                                                                                                                                cTACAAAGGCATACCCATTACGCCTGCCCAATATACAAATAGATCGCCAGAAAGTGGC‑ACACACCACCGCCGAGCACGAAGCCGTGCCAGATGGCATGGTTGTATGGAATGCGTTTGCAGACGTAGAAAATCACCCCGAGCGAATAAACCACGCCGCCTACCGCCAGTAAGGTAACGCTGCCCGCCGCGAGCTTAACTGCCATTTCATAAATTACCACCAGCGACAGCCAGCCCATCGCCAGATAGGTCAc                                           >  1:105639/1‑251 (MQ=255)                                                                                                                                                                                                   aagcctgaccagtctgtaaaTAGATCGCCAGAAAGCGGC‑ACCCACCACCGCCGAGAACGAAGCCGCGCCAGCTGGCATGGTTGTATGGACTGCGTTTGCAGACGTAGCCCATCACACCGCGCGCATAAACCACGCCGCCCACCGCCAGTAAGGTAACGCTGCCCGCCGCGAGCTTACCTGCCATTTCATAAATTACCACCCGCGACAGCCAGCCCATCGCCAGATCGGTCAGCAGAGATAAAATTTTgtat                        >  2:131194/18‑248 (MQ=255)                                                                                                                                                                                                                      aTAGATCGCCAGAAAGTGGC‑ACACACCACCGCCGAGCACGAAGCCGTGCCAGATGGCATGGTTGTATGGAATGCGTTTGCAGACGTAGAAAATCACCCCGAGCGAATAAACCACGCCGCCTACCGCCAGTAAGGTAACGCTGCCCGCCGCGAGCTTAACTGCCATTTCATAAATTACCACCAGCGAcagcca                                                                >  1:143830/1‑192 (MQ=255)                                                                                                                                                                                                                          aTCGCCAGAAAGTGGC‑ACACACCACCGCCGAGCACGAAGCCGTGCCAGATGGCATGGTTGTATGGAATGCGTTTGCAGACGTAGAAAATCACCCCGAGCGAATAAACCACGCCGCCTACCGCCAGTAAGGTAACGCTGCCCGCCGCGAGCTTAACTGCCATTTCATAAATTACCACCAGCGACAGCCAGCCCATCGCCAGATAGGCCACCAGAGATAAAATTTTGAATCGGCGCGCGATGGTCAGTTTaa  >  2:87122/1‑250 (MQ=255)                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                    CTTTATCGCATCAGGCATTTATCGCCTGATGCGACGCTGGCGCGTCTTATCATGTCTGGGATTTGATGCCTTTATCACAACAGGGACGAGGCCCGTAGATCGGATAAGGCGTTC‑ACGCCGAATCCGGCAGTTGTGCTCCGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACAAAGGCATACCCATTACGCCTGCCCAATATACAAATAGATCGCCAGAAAGTGGC‑ACACACTACCGCCGAGCACGAAGCCGTGCCAGATGGCATGGTTGTATGGAATGCGTTTGCAGACGTAGAAAATCACCCCGAGCGAATAAACCACGCCGCCTACCGCCAGTAAGGTAACGCTGCCCGCCGCGAGCTTAACTGCCATTTCATAAATTACCACCAGCGACAGCCAGCCCATCGCCAGATAGGTCACCAGAGATAAAATTTTGAATCGGTGCGCGATGGTCAGTTTAA  >  NC_000913/3042298‑3042763

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑