Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,949,623	G→A	V128I (GTC→ATC)	argA →	N‑acetylglutamate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,949,623	0	G	A	96.4%	90.3 / NA	28	V128I (GTC→ATC)	argA	N‑acetylglutamate synthase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (11/16);  minor base T (0/1);  total (11/17)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GATATCGGGTTGTTGCACAGCCTCGGCATCCGTCTGGTGGTGGTCTATGGCGCACGTCCGCAGATCGACGCAAATCTGGCTGCGCATCACCACGAACCGCTGTATCACAAGAATATACGTGTGACCGACGCCAAAACACTGGAACTGGTGAAGCAGGCTGCGGGAACATTGCAACTGGATATTACTGCTCGCCTGTCGATGAGTCTCAATAACACGCCGCTGCAGGGCGCGCATATCAACGTCGTCAGTGGCAATTTTATTATTGCCCAGCCGCTGGGCGTCGATGACGGCGTGGATTACTGCCATAGCGGGCGTATCCGGCGGATTGATGAAGACGCGATCCATCGTCAACTGGACAGCGGTGCAATAGTGCTAATGGGGCCGGTCGCTGTTTCAGTCACTGGCGAGAGCTTTAACCTGACCTCGGAAGAGATTGCCACTCAACTGGCCATCAAACTGAAAGCTGAAAAGATGATTGGTTTT  >  NC_000913/2949383‑2949865                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                   gATATCGGGTTGTTGCACAGCCTCGGCATCCGTCTGGTGGTGGTCTATGGCGCACGTCCGCAGATCGACGCAAAGCTGGCTGCGCATCACCACGAACCGCTGTATCACAAGAATATACGGGTGACCGACGCCAAAACACTGGAACTGGTGAAGCAGGCTGCGGGAACATTGCAACTGGATATTACTGCTCGCCTGTCGATGAGTCTCAATAACACGCCGCTGCAGGGCGCGCATATCAACATCGTCAGTg                                                                                                                                                                                                                                           <  2:106387/250‑1 (MQ=255)       gggTTGTTGCACAGCCTCGGCATCCGTCTGGTGGTGGTCTATGGCGCACGTCCGCAGATCGACGCAAATCTGGCTGCGCATCACCACGAACCGCTGTATCACAAGAATATACGTGTGACCGACGCCAAAACACTGGAACTGGTGAAGCAGGCTGCGGGAACATTGCAACTGGATATTACTGCTCGCCTGTCGATGAGTCTCAATAACACGCCGCTGCAGGGCGCGCATATCAACATCGTCAGTGGCAAttt                                                                                                                                                                                                                                    <  1:80185/251‑1 (MQ=255)           tgttgCACAGCCTCGGCATCCGTATGGTGGTGGTCTATGGCGCACGTCCGCAGATCGACGCAAATCTGGCTGCGCATCACCACGAACCGCTGTATCACAAGAATATACGTGTGACCGACGCCAAAACACTGGAACTGGTGAAGCAGGCTGCGGGAACATTGCAACTGGATATTACTGCTCGCCTGTCGATGAGTCTCAATAACACGCCGCTGCAGGGCGCGCATATCAACATCGTCAGTGGCAATTttatt                                                                                                                                                                                                                                >  1:72511/1‑251 (MQ=255)             ttgCACAGCCTCGGCATCCGTCTGGTGGTGGTCTATGGCGCACGTCCGCAGATCGACGCAAATCTGGCTGCGCATCACCACGAACCGCTGTATCACAAGAATATACGTGTGACCGACGCCAAAACACTGGAACTGGTGAAGCAGGCTGCGGGAACATTGCAACTGGATATTACTGCTCGCCTGTCGATGAGTCTCAATAACACGCCGCTGCAGGGCGCGCATATCAACATCGTCAGTGGCAATTTtattat                                                                                                                                                                                                                              >  2:211219/1‑251 (MQ=255)                cacaGCCTCGGCATCCGTCTGGTGGTGGTCTATGGCGCACGTCCGCAGATCGACGCAAATCTGGCTGCGCATCACCACGAACCGCTGTATCACAAGAATATACGTGTGACCGACGCCAAAACACTGGAACTGGTGAAGCAGGCTGCGGGAACATTGCAACTGGATATTACTGCTCGCCTGTCGATGAGTCTCAATAACACGCCGCTGCAGGGCGCGCATATCAACATCGTCAGTGGCAATTTTATTATTGc                                                                                                                                                                                                                           >  1:88666/1‑251 (MQ=255)                    gCCTCGGCATCCGTCTGGTGGGGGTCTATGGCGCACGTCCGCAGATCGACGCAAATCTGGCTGCGCATCACCACGAACCGCTGTATCACAAGAATATACGTGTGACCGACGCCAAAACACTGGAACTGGTGAAGCAGGCTGCGGGAACATTGCAACTGGATATTACTGCTCGCCTGTCGATGAGTCTCAATAACACGCCGCTGCAGGGCGCGCATATCAACATCGTCAGTGGCAATTTTATTATTGCCCAg                                                                                                                                                                                                                       <  2:245858/251‑1 (MQ=255)                                    ggtggtggtCTATGGCGCACGTCCGCAGATCGACGCAAATCTGGCTGCGCATCACCACGAACCGCTGTATCACAAGAATATACGTGTGACCGACGCCAAAACACTGGAACTGGTGAAGCAGGCTGCGGGAACATTGCAACTGGATATTACTGCTCGCCTGTCGATGAGTCTCAATAACACGCCGCTGCAGGGCGCGCATATCAACATCGTCAGTGGCAATTTTATTATTGCCCAGCCGCTGGGCGTCGa                                                                                                                                                                                                         <  2:139790/249‑1 (MQ=255)                                         gggtCTATGGCGCACGGCCGCAGATCGACGCAAATCTGGCTGCGCATCACCACGAACCGCTGTATCACAAGAATATACGTGTGACCGACGCCAAAACACTGGAACTGGTGAAGCAGGCTGCGGGAACATTGCAACTGGATATTACTGCTCGCCTGTCGATGAGTCTCAATAACACGCCGCTGCAGGGCGCGCATATCAACATCGTCAGTGGCAATTTTATTATTGCCCAGCCGCTGGGCGTCGATGACGGc                                                                                                                                                                                                  <  2:87946/250‑1 (MQ=255)                                          ggtCTATGGCGCACGTCCGCAGATCGACGCAAATCTGGCTGCGCATCACCCCGAACCGCTGTATCACAAGGATATACGTGTGACCGACGCCAAAACACTGGAACTGGTGAAGCAGGCTGCGGGAACATTGCAACTGGATATTACTGCTCGCCTGTCGATGAGTCTCAATAACACGCCGCTGCAGGGCGCGCATATCAACATCGTCAGTGGCAATTTTATTATTGCCCAGCCGCTGGGCGTCGATGACGGCg                                                                                                                                                                                                 >  1:96986/1‑251 (MQ=255)                                            tCTATGGCGCACGTCCGCAGATCGACGCAAATCTGGCTGCGCATCACCACGAACCGCTGTATCCAAAGAATATACGTGTGACCGACGCCAAAACACTGGAACTGGTGAAGCAGGCTGCGGGAACATTGCAACTGGATATTACTGCTCGCCTGTCGATGAGTCTCAATAACACGCCGCTGCAGGGCGCGCATATCAAAATCGTCAGTGGCAATTTTATTATTGCCCAGCCGCTGGGCGTAGATGCAGGCGTg                                                                                                                                                                                               >  2:207136/1‑251 (MQ=255)                                               aTGGCGCACGTCCGCAGATCGACGCAAATCTGGCTGCGCATCACCACGAACCGCTGTATCACAAGAATATACGTGTGACCGACGCCAAAACACTGGAACTGGTGAAGCAGGCTGCGGGAACATTGCAACTGGATATTACTGCTCGCCTGTCGATGAGTCTCAATAACACGCCGCTGCAGGGCGCGCATATCAACATCGTCAGTGGCAATTTTATTATTGCCCAGCCGCTGGGCGTCGATGACGGCGTgg                                                                                                                                                                                              <  1:117607/249‑1 (MQ=255)                                                              aGATCGACGCAAATCTGGCTGCGCATCACCACGAACCGCTGTATCACAAGAATATACGTGTGACCGACGCCAAAACACTGGAACTGGTGAAGCAGGCTGCGGGAACATTGCAACTGGATATTACTGCTCGCCTGTCGATGAGTCTCAATAACACGCCGCTGCAGGGCGCGCATATCAACATCGTCAGTGGCAATTTTATTATTGCCCAGCCGCTGGGCGTCGATGACGGCGTGGATTACTGCCATAGCggg                                                                                                                                                                             >  1:49449/1‑251 (MQ=255)                                                                   gACGCAAATCTGGCTGCGCATCACCACGAACCGCTGTATCACAAGAATATACGTGTGACCGACGCCAAAACACTGGAACTGGTGAAGCAGGCTGCGGGAACATTGCAACTGGATATTACTGCTCGCCTGTCGATGAGTCTCAATAACACGCCGCTGCAGGGCGCGCATATCAACATCGTCAGTGGCAATTTTATTATTGCCCAGCCGCTGGGCGTCGATGACGGCGTGGATTACTGCCATAGCGGGCGTAt                                                                                                                                                                        >  1:154644/1‑251 (MQ=255)                                                                                        caccacGAACCGCGGTATCACAAGAATACACTTGGGATCGACGCAAAAACACTGGAGCTGGTGAAGCAGGCTGCGGGAACATTGCAACTGGATATTACTGCGCGCCTGGCGATGAGTCTCAATAACACGCCGGTGCAGGGCGCGCATATCAACATCGTCAGTGGCAATTTTAGTATTGCCCAGCCTCTGGGCGGCGATGACGGGGTGGATTACTGCCATAGCGGGCGTATCCGGCGGATTGATGAAGAcg                                                                                                                                                    <  2:178429/250‑1 (MQ=255)                                                                                         accacGAACCGCTGTATCACAAGAATATACGTGTGACCGACGCCAAAACACTGGAACTGGTGAAGCAGGCTGCGGGAACATTGCAACTGGATATTACTGCTCGCCTGTCGATGAGTCTCAATAACACGCCGCTGCAGGGCGCGCATATCAACATCGTCAGTGGCAATTTTATTATTGCCCAGCCGCTGGGCGTCGATGACGGCGTGGATTACTGCCATAGCGGGCGTATCCGGCGGATTGATGAAGAcgcg                                                                                                                                                  >  2:210426/1‑251 (MQ=255)                                                                                                                           gACCGACGCCAAAACACTGGAACTGGTGAAGCAGGCTGCGGGAACATTGCAACTGGATATTACTGCTCGCCTGTCGATGAGTCTCAATAACACGCCGCTGCAGGGCGCGCATATCAACAGCGTCAGTGGCAATTTTATTATTGCCCAGCCGCTGGGCGTCGATGACGGCGTGGATTACTGCCATAGCGGGCGTATCCGGCGGATTGATGAAGACGCGATCCATCGTCAACTGGACAGCGGTGCAATAGTg                                                                                                                 <  2:141728/250‑1 (MQ=255)                                                                                                                                             ggAACTGGTGAAGCAGGCTGCGGGAACATTGCAACTGGATATTACTGCTCGCCTGTCGATGAGTCTCAATAACCCGCCGCTGCAGGGCGCGCATATCAACATCGTCAGTGGCAATTTTATTATTGCCCAGCCGCTGGGCGTCGATGACGGCGTGGATTACTGCCATAGCGGGCGTATCCGGCGGATTGATGAAGACGCGATCCAGCGTCAACTGGACAGCGGTGCAATAGTGCTATTGGGGCCGGTCGCTg                                                                                              <  1:73347/251‑1 (MQ=255)                                                                                                                                                           aGGCTGCGGGAACATTGCAACTGGATATTACTGCTCGCCTGTCGATGAGTCTCAATAACACGCCGCTGCAGGGCGCGCATATCAACATCGTCAGTGGCAATTTTATTATTGCCCAGCCGCTGGGCGTCGATGACGGCGTGGATTACTGCCATAGCGGGCGTATCCGGCGGATTGATGAAGACGCGATCCATCGTCAACTGGACAGCGGTGCAATAGTGCTAATGGGGCCGGTCGATGTTTCAGTCACTGGc                                                                                >  2:253724/1‑251 (MQ=255)                                                                                                                                                                              aCTGGATATTACTTCTCGCCGGTCGATGAGTCTCAAAAACACGCCGCTGCAGGGCGCGCATATCAACACCGTCAGTGGCAATTTTATTAGTGCCCAGCCGCTGGGCGTCGATGACGGCGTGGATTACTGCCATAGCGGGCGTATCCGGCGGATTGATGAAGACGCGATCCATCGTCAACTGGACAGCGGTGCAATAGTGCTAATGGGGCCGGTCGCTGTTTCAGTCACTGGCGAGAGCTTTAACCTGACCt                                                             <  2:239458/251‑1 (MQ=255)                                                                                                                                                                                tGGATATTACTGCTCGCCTGTCGATGAGTCTCAATAACACGCCGCTGCAGGGCGCGCATATCAACATCGTCAGTGGCAATTTTATTATTGCCCAGCCGCTGGGCGTCGATGACGGCGTGGATTACTGCCATAGCGGGCGTATCCGGCGGATTGATGAAGACGCGATCCATCGTCAACTGGACAGCGGTGCAATAGTGCTAATGGGGCCGGTCGCTGTTTCAGTCACTGGCGAGAGCTTTAACCTGACCTCg                                                           >  1:69847/1‑251 (MQ=255)                                                                                                                                                                                           gCTCGCCTGTCGATGAGTCTCAATAACACGCCGCTGCAGGGCGCGCATATCAACATCGTCAGTGGCAATTTTATTATTGCCCAGCCGCTGGGCGTCGATGACGGCGTGGATTACTGCCATAGCGGGCGTATCCGGCGGATTGATGAAGACGCGATCCATCGTCAACTGGACAGCGGTGCAATAGTGCTAATGGGGCCGGTCGCTGTTTCAGTCACTGGCGAGAGCTTTAACCTGACCTCGGAAGAGATTGc                                                <  1:211219/251‑1 (MQ=255)                                                                                                                                                                                                                   aaCACGCCGCTGCAGGGCGCGCATATCAACAGCGTCAGTGGCAATTTTATTATTGCCCAGCCGCTGGGCGTCGATGACGGCGTGGATTACTGCCATAGCGGGCGTATCCGGCGGATTGATGAAGACGCGATCCATCGTCAACTGGACAGCGGTGCAATAGTGCTAATGGGGCCGGTCGCTGTTTCAGTCACTGGCGAGAGCTTTAACCTGACCTCGGAAGAGATTGCCACTCAACTGGCCATCAAACTGaa                        <  2:72511/251‑1 (MQ=255)                                                                                                                                                                                                                     cccgccgcTGCAGGGCGCGGATATCAACTTCGTCATGGGCAATTTTTTGATTGCCAGGCCGCTGGGCGTCGAGGACGGCGTGGATTACTGCCATAGCGGGCGTATCCGGCGGATTGATGAAGACGCGATCCATCGTCAGCTGGACAGGGGTGCAATAGTGCTAATGGGGCCGGTCGCTGTTTCAGTCACTTGCGAGAGCTTTAACCTGACCTCGGAAGAGATTGCCACGCAACTGGCCATCAAATTGAAAg                      <  2:29693/249‑1 (MQ=255)                                                                                                                                                                                                                          cgcTGCAGGGCGCGCATATCAACATCGTCAGTGGCAATTTTATTATTGCCCAGCCGCTGGGCGTCGATGACGGCGTGGATTACTGCCATAGCGGGCGTATCCGGCGGATTGATGAAGACGCGATCCATCGTCAACTGGACAGCGGTGCAATAGTGCTAATGGGGCCGGTCGCTGTTTCAGTCACTGGCGAGAGCTTTAACCTGACCTCGGAAGAGATTGCCACTCAACTGGCCATCAAACTGAAAGCTGa                  >  1:151581/1‑250 (MQ=255)                                                                                                                                                                                                                                  ggCGCGCATATCAACATCGTCAGTGGCAATTTTATTATTGCCCAGCCGCTGGGCGTCGATGACGGCGTGGATTACTGCCATAGCGGGCGTATCCGGCGGATTGATGAAGACGCGATCCATCGTCAACTGGACAGCGGTGCAATAGTGCTAATGGGGCCGGTCGCTGTTTCAGTCACTGGCGAGAGCTTTAACCTGACCTCGGAAGAGATTGCCACTCAACTGGCCATCAAACTGAAAGCTGAAAAgatgat         <  1:64960/251‑1 (MQ=255)                                                                                                                                                                                                                                       gcATATCAACATCGTCAGTGGCAATTTTATTATTGCCCAGCCGCTGGGCGTCGATGACGGCGTGGATTACTGCCATAGCGGGCGTATCCGGCGGATTGATGAAGACGCGATCCATCGTCAACTGGACAGCGGTGCAATAGTGCTAATGGGGCCGGTCGCTGTTTCAGTCACTGGCGTGAGCTTTAACCTGACCTCGGAAGAGATTGCCACTCAACTGGCCATCAAACTGAAAGCTGAAAAGATGATTGGtt    <  1:31108/251‑1 (MQ=255)                                                                                                                                                                                                                                        cATATCAACATCGTCAGTGGCAATTTTATTATTGCCCAGCCGCTGGGCGTCGATGACGGCGTGGATTACTGCCATAGCGGGCGTATCCGGCGGATTGATGAAGACGCGATCCATCGTCAACTGGACAGCGGTGCAATAGTGCTAATGGGGCCGGTCGCTGTTTCAGTCACTGGCGAGAGCTTTAACCTGACCTCGGAAGAGATTGCCACTCAACTGGCCGGCAAACTGAAAGCTGAAAAGATGATTGGttt   <  1:72341/251‑1 (MQ=255)                                                                                                                                                                                                                                         atagcAACATCGTCAGTGGCAATGTTATTAGTGCCCGGCCGCTGGGCGTCGATGACGGCGTGGATTACTGCCATAGCGGGCGTATCCGGCGGATTGATGAAGGCTCGATCCATCGTCAACTGGACAGCGGTGCAATAGTGCTAAGGGGGCCGGTCGCTGTTTCAGTCACTGGCGAGAGCTTTAACCTGACCTCGGAAGAGATTGCCACTCAACTGGCCATCAAACTGAAAGCTGAAAAGATGATTGGtttt  <  1:95300/247‑1 (MQ=255)                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                   GATATCGGGTTGTTGCACAGCCTCGGCATCCGTCTGGTGGTGGTCTATGGCGCACGTCCGCAGATCGACGCAAATCTGGCTGCGCATCACCACGAACCGCTGTATCACAAGAATATACGTGTGACCGACGCCAAAACACTGGAACTGGTGAAGCAGGCTGCGGGAACATTGCAACTGGATATTACTGCTCGCCTGTCGATGAGTCTCAATAACACGCCGCTGCAGGGCGCGCATATCAACGTCGTCAGTGGCAATTTTATTATTGCCCAGCCGCTGGGCGTCGATGACGGCGTGGATTACTGCCATAGCGGGCGTATCCGGCGGATTGATGAAGACGCGATCCATCGTCAACTGGACAGCGGTGCAATAGTGCTAATGGGGCCGGTCGCTGTTTCAGTCACTGGCGAGAGCTTTAACCTGACCTCGGAAGAGATTGCCACTCAACTGGCCATCAAACTGAAAGCTGAAAAGATGATTGGTTTT  >  NC_000913/2949383‑2949865

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑