Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 239,412 C→T intergenic (‑676/+7) ykfM ← / ← yafV uncharacterized protein YkfM/2‑oxoglutaramate amidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913239,4120CT97.1% 107.8 / NA 34intergenic (‑676/+7)ykfM/yafVuncharacterized protein YkfM/2‑oxoglutaramate amidase
Reads supporting (aligned to +/- strand):  ref base C (0/1);  new base T (21/12);  total (21/13)
Fisher's exact test for biased strand distribution p-value = 3.82e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGGTAATGAATGAAGACGACTACAAAATAAGAAGAGGAAACGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTTGACGAATGAGAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAGTCCTTGCGGGGAGCGGGCTTTCGTAGTAA‑TACCCCGACTCTCCCCGT‑CCTTAAACACAACCCCCACTCACCA‑CAACCTAAACTCATCCGCATCCTGCCATGCCGGAAACTTTTCTCTATATTCCCGCAATGCTGCCATCGACAGCTCCGCATCAATGCGCGTTGCCTGATGCGCGTCGGCAGTAGCGATAATCTCTCCTTGCGGATTAATCACCCGGCTGTCACCGCGATAATGGCAGCCGTTGCCATCGCTGCCGACGCGATTGCATCCCGCCACATACGCCTGATTCTCAATCGCGCGGGCCG  >  NC_000913/239185‑239657
                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                      
tGGTAATGAATGAAGACGACTACAAAATAAGAAGAGGAAACGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTTGACGAATGAGAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAGTCCTTGTGGGGAGCGGGCTTTCGTAGTAA‑TACCCCGACTCTCCCCGT‑CCTTAAACACAATCCCCACTCACCA‑CAACCTAAACt                                                                                                                                                                                                                                >  1:36621/1‑251 (MQ=255)
  gTAATGAATGACGCCGAATACAAAATAAGTAGAGGAAACGCAGCAGCATTATTTTCAGGGATAAGGCACATTGCTATTAATCTTTTGACGAATGAGAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAGTCCTTGTGGGGAGCGGGCTTTCGTAGTAA‑TACCCCGACTCTCCCCGT‑CCTTAAACACAATCACCACTCACCA‑CAACCTAAtctcc                                                                                                                                                                                                                              >  2:35438/1‑250 (MQ=18)
       gaatgaaGACGACTACAAAATAAGAAGAGGAAACGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTTGACGAATGAGAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAGTCCTTGTGGGGAGCGGGCTTTCGTAGTAA‑TACCCCGACTCTCCCCGT‑CCTTAAACACAATCCCCACTCACCA‑CAACCTAAATTCATCCGc                                                                                                                                                                                                                         >  1:224397/1‑251 (MQ=255)
                 gacTACAAAATAAGAAGAGGAAACGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTTGACGAATGAGAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAGTCCTTGTGGGGAGCGG‑CCTTCGGAGTAAATACCC‑GGCTCTCCCCGTTCTTTAAACACA‑TCCCCACTCCACA‑CCACCTAAAATCCACCCCATCCTGcccat                                                                                                                                                                                                              >  2:101000/1‑248 (MQ=14)
                         aaTAAGAAGAGGAAACGCAGCAGAATTATTTTCAGGGATAAGGCACATTGCTATTAATATTTTGACGAATGAGAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAGTCCTTGTGGGGAGCGGGCTTTCGTAGTAA‑TACCCCGACTCTCCCCGT‑CCTTAAACACAATCCCCACTCACCA‑CAACCTAAACTCATCCGCATCCTGCCATGCCGGaa                                                                                                                                                                                                        >  1:230781/1‑250 (MQ=255)
                                     aaaCGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTTGACGAATGAGAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAGTCCTTGTGGGGAGCGGGCTTTCGTAGTAA‑TACCCCGACTCTCCCCGT‑CCTTAAACACAATCCCCACTCACCA‑CAACCTAAACTCATCCGCATCCTGCCATGCCGGAAACTTTTCTCtata                                                                                                                                                                                           <  1:111944/251‑1 (MQ=255)
                                     aaaCGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTTGACGAATGAGAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAGTCCTTGTGGGGAGCGGGCTTTCGTAGTAA‑TACCCCGACTCTCCCCGT‑CCTTAAACACAATCCCCACTCACCA‑CAACCTAAACTCATCCGCATCCTGCCATGCCGGAAACTTTTCTCtat                                                                                                                                                                                            >  2:125476/1‑250 (MQ=255)
                                        cGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTTGACGAATGAGAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAGTCCTTGTGGGGAGCGGGCTTTCGTAGTAA‑TACCCCGACTCTCCCCGT‑CCTTAAACACAATCCCCACTCACCA‑CAACCTAAACTCATCCGCATCCTGCCATGCCGGAAACTTTTCTCTATATTc                                                                                                                                                                                        <  1:73544/251‑1 (MQ=255)
                                                           gggATACGGCACATTGCTATTAATATTTTGACGAATGAGAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAGTCCTTGTGGGGAGCGGGCTTTCGTAGTAA‑TACCCCGACTCTCCCCGT‑CCTTAAACACAATCCCCACTCACCA‑CAACCTAAACTCATCCGCATCCTGCCATGCCGGAAACTTTTCTCTATATTCCCGCAATGCTGCCATCGAc                                                                                                                                                                     <  1:181531/251‑1 (MQ=255)
                                                                            tATTAATATTTTGACGAATGAGAAGGTATTCACGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAGTCCTTGTGGGGAGCGGGCTTTCGTAGTAA‑TACCCCGACTCTCCCCGT‑CCTTAAACACAATCCCCACTCACCA‑CAACATAAACTCATCCGCATCCTGCCATGCCGGCAACTTTTCTCTATATTTCCGCAATGCTGCCATCGACAGCTCCGCATCAATgcg                                                                                                                                                    >  2:2413/1‑251 (MQ=255)
                                                                                   atTTTGACGAATGAGAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAGTCCTTGTGGGGAGCGGGCTTTCGTAGTAA‑TACCCCGACTCTCCCCGT‑CCTTAAACACAATCCCCACTCACCA‑CAACCTAAACTCATCCGCATCCTGCCATGCCGGAAACTTTTCTCTATATTCCCGCAATGCTGCCATCGACAGCTCCGCATCAATGCGCGTTGcc                                                                                                                                             >  1:25756/1‑251 (MQ=255)
                                                                                      ttGACGAATGAGAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAGTCCTTGTGGGGAGCGGGCTTTCGTAGTAA‑TACCCCGACTCTCCCCGT‑CCTTAAACACAATCCCCACTCACCA‑CAACCTAAACTCATCCGCATCCTGCCATGCCGGAAACTTTTCTCTATATTCCCGCAATGCTGCCATCGACAGCTCCGCATCAATGCGCGTTGCCTg                                                                                                                                           <  1:110880/250‑1 (MQ=255)
                                                                                         aCGAATGAGAAGGTATTCAAGTCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAGTCCTTGTGGGGAGCGGGCTTTCGTAGTAA‑TACCCCGACTCTCCCCGT‑CCTTAAACACAATCCCCACTCACCA‑CAACCTAAACTCATCCGCATCCTGCCATGCCGGAAACTTTTCTCTATATCCCCGCAATGCTGCCATCGACAGCTCCGCATCAATGCGCGTTGCCTGATgc                                                                                                                                       <  2:44756/251‑1 (MQ=255)
                                                                                         aCGAATGAGAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAGTCCTTGTGGGGAGCGGGCTTTCGTAGTAA‑TACCCCGACTCTCCCCGT‑CCTTAAACACAATCCCCACTCACCA‑CAACCTAAACTCATCCGCATCCTGCCATGCCGGAAACTTTTCTCTATATTCCCGCAATGCTGCCATCGACAGCTCCGCATCAATGCGCGTTGCCTGATg                                                                                                                                        >  2:184053/1‑250 (MQ=255)
                                                                                          cGAATGAGAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGCAAAGCAGCCATGGACAGAAACTACCTGGAGTCAGTCCTTGTGGGGAGCGGGCTTTAGTAGTAA‑TACCCCGACTCTCCCCGT‑CCTTAAACACAATCCCCACTCACCA‑CAACCTAAACTCATCCGCATCCTGCCATGCCGGAAACTTTTCTCTATATTCCCGCAATGCTGCCATCGACAGCTCAGCATCAATGCGCGCTGCCTGATgcg                                                                                                                                      >  1:241335/1‑251 (MQ=38)
                                                                                              tGAGAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCACTGGACAGAAACTACCTGGCGTCCGTCCTTGTGGGGAGCGGGCTTTCGTAGTAA‑TACCCCGACTCTCCCCGT‑CCTTAAACACAATCCCCACTCACCA‑CAACCTAAACTCATCCGCATCCTGCCATGCCGGAAACTTTTCTCTATATTCCCGCACTGCTGCCATCGACAGCTCCGCATCAATGCGCGTTGCCTGATGCGCGTc                                                                                                                                  >  1:251600/1‑251 (MQ=255)
                                                                                                     gTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAGTCCTTGTGGGGAGCGGGCTTTCGTAGTAA‑TACCCCGACTCTCCCCGT‑CCTTAAACACAATCCCCACTCACCA‑CAACCTAAACTCATCCGCATCCTGCCATGCCGGAAACTTTTCTCTATATTccc                                                                                                                                                                                      >  1:24561/1‑192 (MQ=255)
                                                                                                     gTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAGTCCTTGTGGGGAGCGGGCTTTCGTAGTAA‑TACCCCGACTCTCCCCGT‑CCTTAAACACAATCCCCACTCACCA‑CAACCTAAACTCATCCGCATCCTGCCATGCCGGAAACTTTTCTCTATATTccc                                                                                                                                                                                      <  2:24561/192‑1 (MQ=255)
                                                                                                          cAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAGTCCTTGTGGGGAGCGGGCTTTCGTAGTAA‑TACCCCGACTCTCCCCGT‑CCTTAAACACAATCCCCACTCACCA‑CAACCTAAACTCATCCGCATCCTGCCATGCCGGAAACTTTTCTCTATATTCCCGCAATGCTGCCATCGACAGCTCCGCATCAATGCGCGTTGCCTGATGCGCGTCGGCAGTAGCGa                                                                                                                       <  1:92731/250‑1 (MQ=255)
                                                                                                                      aaGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAGTCCTTGTGGGGAGCGGGCTTTCGTAGTAA‑TACCCCGACTCTCCCCGT‑CCTTAAACACAATCCCCACTCACCA‑CAACCTAAACTCATCCGCATCCTGCCATGCCGGAAACTTTTCTCTATATTCCCGCAATGCTGCCATCGACAGCTCCGCATCAATGCGCGTTGCCTGATGCGCGTCGGCAGTAGCGATAATCTCTCCTTg                                                                                                          <  1:19815/251‑1 (MQ=255)
                                                                                                                           gTAAGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAGTACTTGTGGGGAGCGGGCTTTCGTAGTAA‑TACAACGACTCTCCCCGT‑CCTTAAACACAATCCCCACTCACCA‑CAACCTAAACTCATCCGCATCCTGCCATGCCGGAAACTTTTCTCTCTATTCCCGCAATGCTGCCATCGACAGCTCCGCATCAATGCGCGTTGCCTGATGCGCGTCGGCAGTAGCGATAATCTCTCCTTGCGGAt                                                                                                     >  1:112049/1‑251 (MQ=255)
                                                                                                                                      aaggcagcaATGGACAGAACCTCCCTGGCGGCAGTCCTTGTGGGGACCGGGCGTCCGAAGTAA‑TACCCTGACTCTCCCCGT‑CCTTAACCACAACCCCCCCTC‑CCATCAACCTAACCTCACCGGCATCCTGCCATGCCGGAAACTTGTCTCTATATTCCCGCAAGGTTGCCACGGCCAGCTCCGCATCAATGCGCGTTGCCTGATGCGCGTCGGCAGTAGGTATA‑TCTCGCCTTGCGGATTAATCTCCCGGc                                                                                         <  1:180937/248‑1 (MQ=255)
                                                                                                                                      aaaGCAGCCATGGACAGAAACTACCTGGCGTCAGTCCTTGTGGGGAGCGGGCTTTCGTAGTAA‑TACCCCGACTCTCCCCGT‑CCTTAAACACAATCCCCACTCGCCA‑CAACCTAAACTCATCCGCATCCTGCCATGCCGGAAACTTTTCTCTATATTCCCGCAATGCTGCCATCGACAGCTCCGCATCAATGCGCGTTGCCTGATGCGCGTCGGCAGTAGCGATAATCTCTCCTTGCGGATTAATCACCCgg                                                                                          >  1:34771/1‑251 (MQ=255)
                                                                                                                                                            cccTGGCGTCAGGCCGTGTGGGGAGCGGGCTTTCGTAGGAA‑TCCCCCGACTCTCCCCGT‑CCTTAAACACAATCCCCCCTCACCA‑CAACCTAAACTCATCCGCATCCTGCCATGCCGGAAACTTTTCTCTATATTCCCGCAATGCTGCCATCGACAGCTCCGCATCAATGCGCGTTGCCTGATGCGCGTCGGCAGTAGCGATAATCTCTCCTTGCGGATTAATCACCCGGCTGTCACCGCGATAATGGCa                                                                      <  2:216934/248‑1 (MQ=255)
                                                                                                                                                              cTGGCGTCAGTCCTTGTGGGGAGCGGGCTTTCGTAGTAA‑TACCCCGACTCTCCCCGT‑CCTTAAACACAATCCCCACTCACCA‑CAACCTAAACTCATCCGCATCCTGCCATGCCGGAAACTTTTCTCTATATTCCCGCAATGCTGCCATCGACAGCTCCGCATCAATGCGCGTTGCCTGATGCGCGTCGGCAGTAGCGATAATCTCTCCTTGCGGATTAATCACCCGGCTGTCACCGCGATAATGGCAGCCg                                                                  >  1:174000/1‑251 (MQ=255)
                                                                                                                                                                ggCGTCAGTCCTTGTGGGGAGCGGGCTTTCGTAGTAA‑TACCCCGACTCTCCCCGT‑CCTTAAACACAATCCCCACTCACCA‑CAACCTAAACTCATCCGCATCCTGCCATGCCGGAAACTTTTCTCTATATTCCCGCAATGCTGCCATCGACAGCTCCGCATCAATGCGCGTTGCCTGATGCGCGTCGGCAGTAGCGATAATCTCTCCTTGCGGATTAATCACCCGGCTGTCACCGCGATAATGGCAGCCGtt                                                                >  1:35796/1‑251 (MQ=255)
                                                                                                                                                                               ggggAGCGGGCTTTCGTAGTAA‑TACCCCGACTCTCCCCGT‑CCTTAAACACAATCCCCACTCACCA‑CAACCTAAACTCATCCGCATCCTGCCATGCCGGAAACTTTTCTCTATATTCCCGCAATGCTGCCATCGACAGCTCCGCATCAATGCGCGTTGCCTGATGCGCGTCGGCAGTAGCGATAATCTCTCCTTGCGGATTAATCACCCGGCTGTCACCGCGATAATGGCAGCCGTTGCCATCGCTGCCGAc                                                 <  2:25756/251‑1 (MQ=255)
                                                                                                                                                                                  gAGCGGGCTTTCGTAGTAA‑TACCCCGACTCTCCCCGA‑CCTTAAACACAATCCCCACTCACCA‑CAACCTAAACTCATCCGCATCCTGCCATGCCGGAAACTTTTCTCTATATTCCCGAAATGCTGCCATCGACAGCTCCGCATCAATGCGCGTTGCCTGATGCGCGTCGGCAGTAGCGATAATCTCTCCCTGCGGATTAATCCCCCGGATGACCCCGCGATAATGGCAGCCGTTGCCCTCGCTGCCGAcgcg                                              >  2:203706/1‑251 (MQ=255)
                                                                                                                                                                                                       aCCCCCACTCTCACCGT‑CCTTAAACACAATCCCCACTCACCA‑CACCCTAAACTCATCCGCATCCTGCCCTGCCGGAAACTTTTCTCTATATTCCCGCAATGCTGCCATCGACAGCTCCGCATCAATGCGCGTTGCCTGATGCGCGTCGGCAGTAGCGATAATCTCTCCTTGCGGATTAATCACCCGGCTGTCACCGCGATAATGGCAGCCGTTGCCATCGCTGCCGACGCGATTGCATCCCCCCACAtccc                          >  2:133844/1‑248 (MQ=255)
                                                                                                                                                                                                           cGACTCTCCCCGT‑CCTTAAACACAATCCCCACTCACCA‑CAACCTAAACTCATCCGCATCCTGCCATGCCGGAAACTTTTCTCTATATTCCCGCAATGCTGCCATCGACAGCTCCGCATCAATGCGCGTTGCCTGATGCGCGTCGGCAGTAGCGATAATCTCTCCTTGCGGATTAATCACCCGGCTGTCACCGCGATAATGGCAGCCGTTGCCATCGCTGCCGACGCGATTGCATCCCGCCACATACGCCTg                      <  1:125476/251‑1 (MQ=255)
                                                                                                                                                                                                           cGACTCTCCCCGG‑CCTTAAACACAATCCCCACTCACCA‑CAACCTAAACCCATCCGCATCCTGCCATGCCGGAAACTTTTCTCTATATTCCCGCAATGCTGCCATCGACAGCTCCGCATCAATGCGCGTTGCCGGATGCGCGTCGGCAGTAGCGATAATCTCCCCTGGCGGATTAATCACCCGGCTGTCACCGCGATAATGGCAGCGGTTGCCATCGCTGCCGACGCGATTGCATCCCGCCACATACGCCTg                      <  1:35438/251‑1 (MQ=255)
                                                                                                                                                                                                              ctctCCCCGT‑CCTTAAACACAATCCCCACTCACCA‑CAACCTAAACTCATCCGCATCCTGCCATGCCGGAAACTTTTCTCTATATTCCCGCAATGCTGCCATCGACAGCTCCGCATCAATGCGCGTTGCCTGATGCGCGTCGGCAGTAGCGATAATCTCTCCTTGCGGATTAATCACCCGGCTGTCACCGCGATAATGGCAGCCGTTGCCATCGCTGCCGACGCGATTGCATCCCGCCACATACGCCTGAtt                   >  1:36764/1‑251 (MQ=255)
                                                                                                                                                                                                                             aaaCACAATCCCCACTCACCA‑CAACCTAAACTCATCCGCATCCTGCCATGCCGGAAACTTTTCTCTATATTCCCGCAATGCTGCCATCGACAGCTCCGCATCAATGCGCGTTGCCTGATGCGCGTCGGCAGTAGCGATAATCTCTCCTTGCGGATTAATCACCCGGCTGTCACCGCGATAATGGCAGCCGTTGCCATCGCTGCCGACGCGATTGCATCCCGCCACATACGCCTGATTCTCAATCGCGCggg     >  1:152676/1‑251 (MQ=255)
                                                                                                                                                                                                                                cacaATCCCCACTCACCA‑CAACCTAAACTCATCCGCATCCTGCCATGCCGGAAACTTTTCTCTATATTCCCGCAATGCTGCCATCGACAGCTCCGCATCAATGCGCGTTGCCTGATGCGCGTCGGCAGTAGCGATAATCTCTCCTTGCGGATTAATCACCCGGCTGTCACCGCGATAATGGCAGCCGTTGCCATCGCTGCCGACGCGATTGCATCCCGCCACATACGCCTGATTCTCAATCGCGCGGGCCg  >  1:232539/1‑251 (MQ=255)
                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                      
TGGTAATGAATGAAGACGACTACAAAATAAGAAGAGGAAACGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTTGACGAATGAGAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAGTCCTTGCGGGGAGCGGGCTTTCGTAGTAA‑TACCCCGACTCTCCCCGT‑CCTTAAACACAACCCCCACTCACCA‑CAACCTAAACTCATCCGCATCCTGCCATGCCGGAAACTTTTCTCTATATTCCCGCAATGCTGCCATCGACAGCTCCGCATCAATGCGCGTTGCCTGATGCGCGTCGGCAGTAGCGATAATCTCTCCTTGCGGATTAATCACCCGGCTGTCACCGCGATAATGGCAGCCGTTGCCATCGCTGCCGACGCGATTGCATCCCGCCACATACGCCTGATTCTCAATCGCGCGGGCCG  >  NC_000913/239185‑239657

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑