Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,435,632 G→A S2F (TCC→TTC)  truA ← tRNA pseudouridine(38‑40) synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,435,6320GA91.3% 62.0 / ‑3.0 24S2F (TCC→TTC) truAtRNA pseudouridine(38‑40) synthase
Reads supporting (aligned to +/- strand):  ref base G (1/1);  new base A (7/14);  total (9/15)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCACGCCGCGTCTTTGCGCAGCGCGGTTGTTTCGAAATGCACAACCTGCCCGGTACCGTGTACCCCTGCGTCAGTACGCCCGGCGCAGAAGACGGTGATGGGTTCGTTCGCCACCTGGGAGAGCGCCTTTTCCAGCTTCTCCTGCACACTGCGGACTTCGTTCTGCCGTTGCCAGCCGTAATACTTACTGCCGTCGTACTCAATGCCCAGCGCAATTTTATAAACTGGCGGTTGTTGCTGGTCGGACATTAGTACAGATACTCCT‑GCACCAGTTTCTCGGCG‑ATTTTTACTGCCATCAGCGCGCCGCCAAAGCGAACGTTATCGGCCACCGACCAGAACTGGACTTGCTCCGGCATACCGTAGTCATTACGCACGCAGCCAACAGAAAGATGCGGCGTACCCGAAGCATCACCTACCTGAGTTGGGAATTCGTTCTCTTCAGAGAGCACAATATCTTCGCCTTGAACAAACGCATCACGCGCTTC  >  NC_000913/2435388‑2435872
                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                  
ccACGCCGCGTCTTTGCGCAGCGCGGTTGTTTCGAAATGCACAACCTGCCCGGTACCGTGTACCCCTGCGTCAGTACGCCCGGCGCAGAAGACGGTGATGGGTTCGTTCGCCACCTGGGAGAGCGCCTTTTCCAGCTTCTCCTGCACACTGCGGACTTCGTTCTGCCGTTGCCAGCCGTAATACTTACTGCCGTCGTACTCAATGCCCAGCGCAATTTTATAAACTGGCGGTTGTTGCTGGTCGAACAtt                                                                                                                                                                                                                                               <  1:220281/250‑1 (MQ=255)
 cACGCCGCGTCTTTGCGCAGCGCGGTTGTTTCGAAATGCACAACCTGCCCGGTACCGTGTACCCCTGCGTCAGTACGCCCGGCGCAGAAGACGGTGATGGGTTCGTTCGCCACCTGGGAGAGCGCCTTTTCCAGCTTCTCCTGCACACTGCGGACTTCGTTCTGCCGTTGCCAGCCGTAATACTTACTGCCGTCGTACTCAATGCCCAGCGCAATTTTATAAACTGGCGGTTGTTGCTGGTCGAACATTAg                                                                                                                                                                                                                                             <  1:45640/251‑1 (MQ=255)
   cgccgcGTCTTTGCGCAGCGCTGTTGTTTCGAAATGCACAACCTGCCCGGTACCGTGTACCCCTGCGTCAGTACGCCCGGCGCAGAATACGGTGATGGGTTCGTTCGCCACCTGGGAGAGCGCCGTTTCCAGCTTCTCCTGCACACTGCGGACTTCGTTCTGCCGTTGCCAGCCGTAATACTTACTGCCGTCGTACTCAATGCCCAGCGCAATTTTATAAACTGGCGGTTGTTGCTGGTCGAACATTAGTa                                                                                                                                                                                                                                           <  2:169105/251‑1 (MQ=255)
                cgcAGCGCGGTTGTTTCGAAAGGCACAACCTGCCCGGTACCGTGTACCCCTGCGTCAGTACGCCCGGCGCAGAAGACGGTGATGGGTTCGTTCGCCACCTGGGAGAGCGCCTTTTCCAGCTTCTCCTGCACACTGCGGACTTCGTTCTGCCGTTGCCAGCCGTAATACTTACTGCCGTCGTACTCAATGCCCAGCGCAATTTTATAAACTGGCGGTTGTTGCTGGTCGAACATTAGTACAGATACTCCT‑Gc                                                                                                                                                                                                                             <  2:56409/251‑1 (MQ=255)
                       cgGTTGTTTCGAAATGCACAACCTGCCCGGTACCGTGTACCCCTGCGTCAGTACGCCCGGCGCAGAAGACGGTGATGGGTTCGTTCGCCACCTGGGAGAGCGCCTTTTCCAGCTTCTCCTGCACACTGCGGACTTCGTTCTGCCGTTGCCAGCCGTAATACTTACTGCCGTCGTACTCAATGCCCAGCGCAATTTTATAAACTGGCGGTTGTTGCTGGTCGAACATTAGTACAGATACTCCT‑GCACCAGtt                                                                                                                                                                                                                      <  1:23291/251‑1 (MQ=255)
                            gttgcGAAATGCACAACCTGCCCGGTACCGTGTACCCCTGCGTCAGTACGCCCGGCGCAGAAGACGGTGATGGGTTCGTTCGCCACCTGGGAGAGCGCCTTTTCCAGCTTCTCCTGCACACTGCGGACTTCGTTCTGCCGTTGCCAGCCGTAATACTTACTGCCGTCGTACTCAATGCCCAGCGCAATTTTATAAACTGGCGGTTGTTGCTGGTCGAACATTAGTACAGATACTCCT‑GCACCAGTTtctc                                                                                                                                                                                                                  <  2:206290/246‑1 (MQ=255)
                            gttTCGAAATGCACACCCTGCCCGGTACCGTGTACCCCTGCGTCAGTACGCCCGGCGCAGAAGACGGTGATGGGTTCGTTCGCCACCTGGGAGAGCGCCTTTTCCAGCTTCTCCTGCACACTGCGGACTTCGTTCTGCCGTTGCCAGCCGTAATACTTACTGCCGTCGTACTCAATGCCCAGCGCAATTTTATAAACTGGCGGTTGTTGCTGGTCGGACATTAGTACAGATACTCCT‑GCACCAGTTTCTCg                                                                                                                                                                                                                 <  2:51702/251‑1 (MQ=255)
                                cGAAATGCACAACCTGCCCGGTACCGTGTACCCCTGCGTCAGTACGCCCGGCGCAGAAGACGGTGATGGGTTCGTTCGCCACCTGGGAGAGCGCCTTTTCCAGCTTCTCCTGCACACTGCGGACTTCGTTCTGCCGTTGCCAGCCGTAATACTTACTGCCGTCGTACTCAATGCCCAGCGCAATTTTATAAACTGGCGGTTGTTGCCGGTCGAACATTAGTACAGATACCCCT‑GCCCCAGGTTCTCGGCgg                                                                                                                                                                                                             >  2:56577/1‑250 (MQ=255)
                                 gAAATGCACAACCGGCCCGGTACCGTGTACCCCTGCGTCAGTACGCCCGGAGCAGAAGACGGTGATGGTTTCGTTCGCCACCTGGGAGAGCGCCTTTTCCAGCTTCTCCTGCACACTGCGGACTTCGTTCTGCCGTTGCCAGCCGTAATACTTACTGACGTCGTACTCACTGCCCAGAGCAATTTTATAAACTGGCGGTTGTTGCTGGTGGAACATTAGTACAGTAACTCCTCGCACC‑GGTGCTCGGCG‑At                                                                                                                                                                                                           >  2:203557/1‑251 (MQ=255)
                                       cacaACCTGCCCGGTACCGTGTACCCCTGCGTCAGTACGCCCGGCGCAGAAGACGGTGATGGGTTCGTTCGCCACCTGGGAGAGCGCCTTTTCCAGCTTCTCCTGCACACTGCGGACTTCGTTCTGCCGTTGCCAGCCGTAATACTTACTGCCGTCGTACTCAATGCCCAGCGCAATTTTATAAACTGGCGGTTTTTGCTGGTCGAACATTAGTACAGAAACTCCT‑GCACCAGTTTTCCGGCGGAtttttc                                                                                                                                                                                                      >  1:260919/1‑250 (MQ=255)
                                                     tACCGTGTACCCCTGCGTCAGTACGCCCGGCGCAGAAGACGGTGATGGGTTCGTTCGCCACCTGGGAGAGCGCCTTTTCCAGCTTCTCCTGCACACTGCGGACTTCGTTCTGCCGTTGCCAGCCGTAATACTTACTGCCGTCGTACTCAATGCCCAGCGCAATTTTATAAACTGGCGGTTGTTGCTGGTCCAACATTAGTACAGATACTCCC‑GCACCAGTTTCTCGGCG‑ATTTTTACTGCCATCAgcgcgc                                                                                                                                                                                       >  2:78965/1‑251 (MQ=255)
                                                                                           aCGGTGATGGGTTCGTTCGCCACCTGGGAGAGCGCCTTTTCCAGCTTCTCCTGCACACTGCGGACTTCGTTCTGCCGTTGCCAGCCGTAATACTTACTGCCGTCGTACTCAATGCCCAGCGCAATTTTATAAACTGGCGGTTTTTGGTGGTCGGACATTTGTACAGATACTCCT‑GCACCAGTTTCTCGGCG‑ATTTTTTCTGCCATCCGCGCGCCGCCAAAGCGAACGTTATCCGCCCCCgccccgacaggg                                                                                                                                                 >  2:65871/1‑240 (MQ=255)
                                                                                                              ccACCTGGGAGAGCGCCTTTTCCAGCTGCTCCGGCACACTGCGGACTTCGTTCTTCCGTTGCCAGCCGTAATACTTACTGCCGTCGTACTCAATGCCCAGCGCAATTTTATAAACTGGCGGTTGTTGCTGGTCGAACATTAGTACAGATACTCCT‑GCACCAGTTTCTCGGCG‑ATTTTTACTGCCAGCAGCGTGCCGCCAAAGCGAACGTTATCGGCCACCGACCTTAACTGGACTTGCTCCGGCATACCGt                                                                                                                              <  2:39668/251‑1 (MQ=255)
                                                                                                                                   ccAGCTTCTACTGCTCACTGCGGACTTCGTTCTGCCGTTGCCAGCCGTAATACTTACTGCCGTCGTACTCAATGCCCAGCGCAATTTTATAAACTGGCGGTTGTTGCTGGTCGAACATTAGTACAGATACTCCT‑GCACCAGTTTCTCGGCG‑ATTTTTACTGCCATCAGCGCGCCGCCAAAGCGAACGTTATCGGCCACCGACCAGAACTGGACTTGCTCCGGCATACCGTAGTCATTACGCACGCAGCCaa                                                                                                         <  2:116292/251‑1 (MQ=255)
                                                                                                                                                     tGCGGACTTCGTTCTGCCGTTGCCAGCCGTAATACTTACTGCCGTCGTACTCAATGCCCAGCGCAATTTTATAAACTGGCGGTTGTTGCTGGTCGAACATTAGTACAGATACTCCT‑GCACCAGTTTCTCGGCG‑ATTTTTACTGCCATCAGCGCGCCGCCAAAGCGAACGGTATCGGCCACCGACCAGAACTGGACTTGCTCCGGCATACCGCAGTCATTACGCACGCAGCCAACAGAAAGATGCGGCGTAc                                                                                       >  1:193845/1‑251 (MQ=255)
                                                                                                                                                                    gCCGTTGCCAGCCGTAATACTTACTGCCGTCGTACTCAATGCCCAGCGCAATTTTATAAACTGGCGGTTGTTGCTGGTCGCACATTAGTCCAGATACTCCT‑GCCCCCGTTTCTCGGCG‑ATTTTTTCTGCCATCAGCGCGCCGCCAAAAGGAAAGTTATCGGGCACCCACCAGAAATGGGCTTGCTCCGGCATACCCGAATCATTACGCACGCAACCAACAGAAAGATGCGGCGTACCCGAAGCCTCacctc                                                                        >  1:135795/1‑250 (MQ=255)
                                                                                                                                                                            cAGCCGTAATACTTACTGCCGTCGTACTCAATGCCCAGCGCAATTTTATAAACTGGCGGTTGTTGCTGGTCGAACATTAGTACAGATACTCCT‑GCACCAGTTTCTCGGCG‑ATTTTTACTGCCATCAGCGCGCCGCCAAAGCGAACGTTATCGGCCACCGACCAGAACTGGACTTGCTCCGGCATACCGTAGTCATTACGCACGCAGCCAACAGAAAGATGCGGCGTACCCGAAGCATCACCTACCTGAGtt                                                                <  1:56577/251‑1 (MQ=255)
                                                                                                                                                                                             gCCGTCGTACTCAATGCCCAGCGCAATTTTATAAACTGGCGGTTGTTGCTGGTCGAACATTAGTACAGATACTCCT‑GCACCAGTTTCTCGGCG‑ATTTTTACTGCCATCAGCGCGCCGCCAAAGCTAACGTTATCGGCCTCCGACCAGAACTGGACTTGCTCCTGCATACCGTAGGCATTACGCACGCAGCCAACAGAAAGATGCGGCGTACCCGAAGCATCACCTACCTGAGTTGGGAATTCGTTCTCtt                                                <  1:155025/250‑1 (MQ=255)
                                                                                                                                                                                                 tcgtACTCAATGCCCAGCGCAATTTTATAAACTGGCGGTTTGTGCTGGTCGAACATTAGTACCGATACTTCT‑GCACCAGTTTCTCGGCG‑ATTTTTAATGCCCTCAGCGCGCCGCCAAAGCGAACGTTATCGGCCCCCGCCCCGAACTGGACTTGCCCCGGCATACCGTAGTCATTACGCACGCAGACAACAGAGAGATGCGGCGGACCCGAAGCATCCCCTCCCTGAGTTGGGAATTCGTTCTCTTCag                                             >  2:70773/1‑249 (MQ=255)
                                                                                                                                                                                                     aCTCAATGTCCAGCGCAATTTTATAAACTGGCTGTTGTTGCTGGTCGAACATTAGTACCGATACTCCT‑GCACTAGTTTCTCGGCG‑ATTTTTACTGCCAGCAGCGCGCCGCCAACGCGAACGTTCTCGGCCACCGACCAGAACTGGACTTGCTCCGACATACCGTAGTCATTACGCACGCAGCCAACAGAAAGATTCTGCGTTCCCGAAGCATCTCCTACCTGAGTTGGTAAGTCGTTCTCGTCAGAGAGc                                        <  1:177305/250‑1 (MQ=255)
                                                                                                                                                                                                                              aaCTGGCGGTTGTTGCTGGTCGAACATTAGTACAGATACTCCT‑GCACCAGTTTCTCGGCG‑ATTTTTACTGCCATCAGCGCGCCGCCAAAGCGAACGTTATCGGCCACCGACCAGAACTGGACTTGCTCCGGCATACCGTAGTCATTACGCACGCAGCCAACAGAAAGATGCGGCGTACCCGAAGCATCACCTACCTGAGTTGGGAATTCGTTCTCTTCAGAGAGCACAATATCTTCGCCTTGAACAAACGc              <  1:184353/251‑1 (MQ=255)
                                                                                                                                                                                                                                 tGGCGGTTGTTGCTGGTCGAACATTAGTACAGATACTCCT‑GCACCAGTTTCTCGGCG‑ATTTTTACTGCCATCAGCGCGCCGCCAAAGCGAACGTTATCGGCCACCGACCAGAACTGGACTTGCTCCGGCATACCGTAGTCATTACGCACGCAGCCAACAGAAAGATGCGGCGTACCCGAAGCATCACCTACCTGAGTTGGGAATTCGTTCTCTTCAGAGAGCACAATATCTTCGCCTTGAACAAACGCATc           >  1:9211/1‑251 (MQ=255)
                                                                                                                                                                                                                                         gttgCTGGTCGAACATTAGTACAGATACTCCT‑GCACCAGTTTCTCGGCG‑ATTTTTACTGCCATCAGCGCGCCGCCAAAGCGAACGTTATCGGCCACCGACCAGAACTGGACTTGCTCCGGCATACCGTAGTCATTACGCACGCAGCCAACAGAAAGATGCGGCGTACCCGAAGCATCACCTACCTGAGTTGGGAATTCGTTCTCTTCAGAGAGCACAATATCTTCGCCTTGAACAAACGCATCACGCGctt   <  1:231144/251‑1 (MQ=255)
                                                                                                                                                                                                                                           tgCTGGTCGAACATTAGTACATATACTCCT‑GCGCCAGTTTCTCGGCG‑AGTTTTACTGCCATCAGCGCGCCGCCAAAGCGAACGTTATCGGCCACCGACCAGAACTGGACTTGCTCCGGCATACCGTAGTCATTACGCACGCAGCCAACAGAAAGATTCGGCGTTCCCGAATCATCACCTACCTGAGTTGGGAATTCGTTCTCTTCAGAGAGCACAATATCTTCGCCTTGAACAAACGCATCACGCGcttc  <  1:232589/250‑1 (MQ=255)
                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                  
CCACGCCGCGTCTTTGCGCAGCGCGGTTGTTTCGAAATGCACAACCTGCCCGGTACCGTGTACCCCTGCGTCAGTACGCCCGGCGCAGAAGACGGTGATGGGTTCGTTCGCCACCTGGGAGAGCGCCTTTTCCAGCTTCTCCTGCACACTGCGGACTTCGTTCTGCCGTTGCCAGCCGTAATACTTACTGCCGTCGTACTCAATGCCCAGCGCAATTTTATAAACTGGCGGTTGTTGCTGGTCGGACATTAGTACAGATACTCCT‑GCACCAGTTTCTCGGCG‑ATTTTTACTGCCATCAGCGCGCCGCCAAAGCGAACGTTATCGGCCACCGACCAGAACTGGACTTGCTCCGGCATACCGTAGTCATTACGCACGCAGCCAACAGAAAGATGCGGCGTACCCGAAGCATCACCTACCTGAGTTGGGAATTCGTTCTCTTCAGAGAGCACAATATCTTCGCCTTGAACAAACGCATCACGCGCTTC  >  NC_000913/2435388‑2435872

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑