Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,434,316	G→A	A142A (GCC→GCT)	dedA ←	DedA family protein DedA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,434,316	0	G	A	100.0%	76.2 / NA	23	A142A (GCC→GCT)	dedA	DedA family protein DedA
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (13/10);  total (13/10)

GTGACGGATTATTTCGATGACGCCCGGCAAAATAGAAACCACAATAATCCCGACGATCAGCAGCTTAAGGTTATCCTGAACCATCGGGATTGTACCGAAGAAATAGCCTGCGTAGGTAAAAAGCAGTACCCACAACAGTGCGCCGATCACGTTATAAGCGGCGAAATGACGGTACGACATGTGGCCCATTCCCGCAACAAACGGGGCGAACGTTCTGACGATCGGCACAAAACGGGCGAGAATAATCGTTTTGCCGCCATGTTTCTCATAAAACTGATGGGTTTTGTCGAGATAACTGCGACGGAAAATTTTCGAATTGGGATTACTGAACAACTTTTCACCGAACAACCGCCCGATAGTGTAATTGACCGCGTCACCCACAATCGCGGCGATCAGCATCAGCACCACCATCATATGGACGTTGAGATCGTTGGTTTCCAGTGATG  >  NC_000913/2434112‑2434557                                                                                                                                                                                                             |                                                                                                                                                                                                                                                  gtgACGGATTATTTCGATGACGCCCGGCAAAATAGAAACCACAATAATCCCGACGATCAGCAGCTTAAGGTTATCCTGAACCATCGGGATTGTACCGAAGAAATAGCCTGCGTAGGTAAAAAGCAGTACCCACAACAGTGCGCCGATCACGTTATAAGCGGCGAAATGACGGTACGACATGTGGCCCATTCCCGCAACAAACGGAGCGAACGTTCTGACGATCGGCACAAAACGGGCGAGAATAATCGttt                                                                                                                                                                                                     <  1:181632/251‑1 (MQ=255)  tgACGGATTATTTCGATGACGCCCGGCAAAATAGAAACCACAATAATCCCGACGATCAGCAGCTTAAGGTTATCCTGAACCATCGTGATTGTACCGAAGAAATAGCCTGCGTAGGTAAAAAGCAGTACCCACAACAGTGCGCCGATCACGTTATAAGCGGCGAAATGACGGTACGACATGTGGCCCATTCCCGCAGCAAACGGAGCGAACGTTCTGACGATCg                                                                                                                                                                                                                                <  2:235878/223‑1 (MQ=255)  tgACGGATTATTTCGATGACGCCCGGCAAAATAGAAACCACAATAATCCCGACGATCAGCAGCTTAAGGTTATCCTGAACCATCGGGATTGTACCGAAGAAATAGCCTGCGTAGGTAAAAAGCAGTACCCACAACAGTGCGCCGATCACGTTATAAGCGGCGAAATGACGGTACGACATGTGGCCCATTCCCGCAACAAACGGAGCGAACGTTCTGACGATCg                                                                                                                                                                                                                                >  1:235878/1‑223 (MQ=255)                                 gaGAAACCACAATAATCCCGACGATCAGCAGCGTAAGGTTATCCTGAACCATCGGGATTGTACCGAAGAAATAGCCTGCGTAGGTAAAAAGCAGTACTCACAACAGTGCGCCGATCACGTTATAAGCGGCGAAATGACGGTACGACATGTGGCCCATTCCCGCAGCAAACGGAGCGAACGTGCTGACGATCGGCACAAAACGGGCGAGAATAATCGTTTTGCCGCCATGTTTCGCATAAAACTGATGGGtt                                                                                                                                                                     <  1:39323/250‑1 (MQ=255)                                                          cagcagCTTAAGGTTATCCTGAACCATCGGGATTGTACCGAAGAAATAGCCTGCGTAGGTAAAAAGCAGTACCCACAACAGTGCGCCGATCACGTTATAAGCGGCGAAATGACGGTACGACATGTGGCCCATTCCCGCAACAAACGGAGCGAACGTTCTGACGATCGGCACAAAACGGGCGAGAATAATCGTTTTGCCGCCATGTTTCTCATAAAACTGATGGGTTTTGTCGAGATAACTGCGACGGaaa                                                                                                                                             >  2:196818/1‑250 (MQ=255)                                                                      gTTATCCTGAACCATCGGGATTGTACCGAAGAAATAGCCTGCGTAGGTAAAAAGCAGTACCCACAACAGTGCGCCGATCACGTTATAAGCGGCGAAATGACGGTACGACATGTGGCCCATTCCCGCAACAAACGGAGCGAACGTTCTGACGATCGGCACAAAACGGGCGAGAATAATCGTTTTGCCGCCATGTTTCTCATAAAACTGATGGGTTTTGTCGAGATAACTGCGACGGAAAATTTTCGaa                                                                                                                                    <  1:122229/247‑1 (MQ=255)                                                                      gTTATCCTGAACCATCGGGATTGTACCGAAGAAATAGCCTGCGTAGGTAAAAAGCAGTACCCACAACAGTGCGCCGATCACGTTATAAGCGGCGAAATGACGGTACGACATGTGGCCCATTCCCGCAACAAACGGAGCGAACGTTCTGACGATCGGCACAAAACGGGCGAGAATAATCGTTTTGCCGCCATGTTTCTCATAAAACTGATGGGTTTTGTCGAGATAACTGCGACGGAAAATTTTCGaa                                                                                                                                    >  2:122229/1‑247 (MQ=255)                                                                         aTCCTGAACCATCGGGATTGTACCGAAGAAATAGCCTGCGTAGGTAAAAAGCAGTACCCACAACAGTGCGCCGATCACGTTATAAGCGGCGAAATGACGGTACGACATGTGGCCCATTCCCGCAACAAACGGAGCGAACGTTCTGACGATCGGCACAAAACGGGCGAGAATAATCGTTTTGCCGCCATGTTTCTCATAAAACTGATGGGTTTTGTCGAGATAACTGCGACGGAAAATTTTCGAATTGGGAt                                                                                                                             >  1:218222/1‑251 (MQ=255)                                                                         aTCCTGAAACATCGGGATTGTACCGACGAAATAGCCTGCGTAGGTAAAAAGCAGTACCCACAACAGTGCGCCGATCACGTTATAAGCGGCGAAATGACGGTACGACATGTGGCCCATTCCCGCAACAAACGGAGCGAACGTTCTGACGATCGGCACAAAACGGGCGAGAATAATCGTTTTGCCGCCATGTTTCTCATAAAACTGATGGGTTTTGTCGAGATAACTGCGACGGAAAATTTTCGAATTGGGAt                                                                                                                             >  2:124671/1‑251 (MQ=255)                                                                               aaCCATCGGTATTGTACCGAAGAAATAGCCTGCGTAGGTAAAAAGCAGTACCCACAACAGTGCGCCGATCCCGTTATAAGCGGCGAAATGACGGTACGACATGTGGCCCATTCCCGCAACAAACGGAGCGAACGTTCTGACGATCGGCACAAAACGGGCGAGAATAATCGTTTTGCCGCCATGTTTCTCATAAAACTGATGGGTTTTGTCGAGATAACTGCGACGGAAAATTTTCGAATTGGGATTACTGa                                                                                                                       <  2:15723/251‑1 (MQ=255)                                                                                     cGGGATTGTACCGAAGAAATAGCCTGCGTAGGTAAAAAGCAGTACCCACAACAGTGCGCCGATCACGTTATAAGCGGCGAAATGACGGTACGACATGTGGCCCATTCCCGCAACAAACGGAGCGAACGTTCTGACGATCGGCACAAAACGGGCGAGAATAATCGTTTTGCCGCCATGTTTCTCATAAAACTGATGGGTTTTGTCGAGATAACt                                                                                                                                                       >  2:199766/1‑213 (MQ=255)                                                                                     cGGGATTGTACCGAAGAAATAGCCTGCGTAGGTAAAAAGCAGTACCCACAACAGTGCGCCGATCACGTTATAAGCGGCGAAATGACGGTACGACATGTGGCCCATTCCCGCAACAAACGGAGCGAACGTTCTGACGATCGGCACAAAACGGGCGAGAATAATCGTTTTGCCGCCATGTTTCTCATAAAACTGATGGGTTTTGTCGAGATAACt                                                                                                                                                       <  1:199766/213‑1 (MQ=255)                                                                                                     aaaTAGCCTTCGTAGGTAAAAAGCAGTACCCACAACAGTGCGCCGATCACTGTATAAGCGGCGAAATGACGGTACGACTTGTGGCCCATTCCCGCAACAAACGGAGCGAACGTTCTGACGATCGGCACAAAACGGGCGAGAATAAGCGTTTTGCCGCCATGTTTCTCATAAAACTGATTGGTTTTGTCGAGATAACTGCGACGGAAAATTTTCTAATTGGGATTACTGAACAAGTTTTCACCGAACAACCg                                                                                                 <  2:211605/251‑1 (MQ=255)                                                                                                                    gTAAAAAGCAGTACCCACAACAGTGCGCCGATCACGTTATAAGCGGCGAAATGACGGTACGACATGTGGCCCATTCCCGCAACAAACGGAGCGAACGTTCTGACGATCGGCACAAAACGGGCGAGAATAATCGTTTTGCCGCCATGTTTCTCATAAAACTGATGGGTTTTGTCGAGATAACTGCGACGGAAAATTTTCGAATTGGGATTACTGAACAACTTTTCACCGAACAACCGCCCGATAGTGTAAtt                                                                                  >  1:32950/1‑251 (MQ=255)                                                                                                                        aaaGCAGTACCCACAACAGTGCGCCGATCACGTTATAAGCGGCGAAATGACGGTACGACATGTGGCCCATTCCCGCAACAAACGGAGCGAACGTTCTGACGATCGGCACAAAACGGGCGAGAATAATCGTTTTGCCGCCATGTTTCTCATAAAACTGATGGGTTTTGTCGAGATAACTGCGACGGAAAATTTTCGAATTGGGATTACTGAACAACTTTTCACCGAACAACCGCCCGATAGTGTAATTGAcc                                                                              >  1:172582/1‑251 (MQ=255)                                                                                                                               tACCCACAAAAGTGCGCCGATCACGTTATAAGCGGCGAAATGACGGCACGACATGTGGCCCATTCCCGCAACAAACGGAGCGAACGTTCTGACGATCGGCACAAAACGGGCGAGAATAATCGTTTTGCCGCCATGTTTCTCATAAAACTGATGGGATTTGTCGAGATAACTGCGACGGAAAATTTTCGAATTGGGATTACTGAACAACTTTTCACCGAACAACCGCCCGATAGTGAAATTGACCGCGTCAc                                                                       >  2:72728/1‑251 (MQ=255)                                                                                                                                    acaacaGTGCGCCGATCACGTTATAAGCGGCGAAATGACGGTACGACATGTGGCCCATTCCCGCAACAACCGGAGCGAACGTTCTGACGCTCGGCACAAAACGGGCGAGAATAATCGTTTTGCCGCCATGTTTCTCATAAAACTGATGGGTTTTGTCGAGATAACTGCGACGGAAAATTTTCGAATTGGGATTACTGAACAACTTTTCACCGAACAACCGCCCGATAGTGTAATTGACCGCGTCACCcac                                                                   >  1:150832/1‑250 (MQ=255)                                                                                                                                       acaGTGCGCCGATCACGTTATAAGCGGCGAAATGACGGTACGACATGTGGCCCATTCCCGCAACAAACGGAGCGAACGTTCTGACGATCGGCACAAAACGGGCGAGAATAATCGTTTTGCCGCCATGTTTCTCATAAAACTGATGGGTTTTGTCGAGATAACTGCGACGGAAAATTTTCGAATTGGGATTACTGAACAACTTTTCACCGAACAACCGCCCGATAGTGTAATTGACCGCGTCACCCACAATc                                                               <  1:196818/251‑1 (MQ=255)                                                                                                                                                       gTTATAAGCGGCGAAATGACGGTACGACATGTGGCCCATTCCCGCAACAAACGGAGCGAACGTTCTGACGATCGGCACAAAACGGGCGAGAATAATCGTTTTGCCGCCATGTTTCTCATAAAACTGATGGGTTTTGTCGAGATAACTGCGACGGAAAATTTTCGAATTGGGATTAATGAACAACTTTTCACCGAACAACCGCCCGATAGTGTAATTGACCGCGTCACCCACAATCGCGGCGatcagcatc                                                >  1:163379/1‑250 (MQ=255)                                                                                                                                                            aaGCGGCGAAATGACGGTACGACATGTGGCCCATTCCCGCAACAAACGGAGCGAACGTTCTGACGATCGGCACAAAACGGGCGAGAATAATCGTTTTGCCGCCATGTTTCTCATAAAACTGATGGGTTTTGTCGAGATAACTGCGACGGAAAATTTTCTAATTGGGATTACTGAACAACTTTTCACCGAACAACCTCCCGATAGTGTAATTGACCGCGTCACCCACAATCGCGGCGATCAGCATCAGcacc                                          <  1:148555/251‑1 (MQ=255)                                                                                                                                                                    aaaTGACGGTACGACATGTGGCCCATTCCCGCAACAAACGGAGCGAACGTTCTGACGATCGGCACAAAACGGGCGAGAATAATCGTTTTGCCGCCATGTTTCTCATAAAACTGATGGGTTTTGTCGAGATAACTGCGACGGAAAATTTTCGAATTGGGATTACTGAACAACTTTTCACCGAACAACCGCCCGATAGTGTAATTGACCGCGTCACCCACAATCGCGGCGATCAGCATCAGCACCACcatcat                                  >  2:196320/1‑251 (MQ=255)                                                                                                                                                                        gACGGTACGACATGTGGCCCATTCCCGCAACAAACGGAGCGAACGTTCTGACGATCGGCACAAAACGGGCGAGAATAATCCTTTTGCCGCCATGTTTCTCATAAATCTGATGGGGTTTGGCGAGATAACTTCGACGGAAAATTTTCGAATTGGGATTACTGAACAACTTTTCGCCGAACATCCGCCCGATAGTGTAATTGACCGCGTCACCCACAATCGCGGCGATCAGCATCAGCACCACCATCATATg                               <  1:254264/250‑1 (MQ=255)                                                                                                                                                                                                    aacaaACGGAGCGAACGTTCTGACGATCGGCACAAAACGGGCGAGAATAATCGTTTTGCCGCCATGTTTCTCATAAAACTGATGGGTTTTGTCGAGATAACTGCGACGGAAAATTTTCGAATTGGGATTACTGAACAACTTTTCACCGAACAACCGCCCGATAGTGTAATTGACCGCGTCACCCACAATCGCGGCGATCAGCATCAGCACCACCATCATATGGACGTTGAGATCGTTGGTTTCCAGTGATg  >  1:43780/1‑251 (MQ=255)                                                                                                                                                                                                             |                                                                                                                                                                                                                                                  GTGACGGATTATTTCGATGACGCCCGGCAAAATAGAAACCACAATAATCCCGACGATCAGCAGCTTAAGGTTATCCTGAACCATCGGGATTGTACCGAAGAAATAGCCTGCGTAGGTAAAAAGCAGTACCCACAACAGTGCGCCGATCACGTTATAAGCGGCGAAATGACGGTACGACATGTGGCCCATTCCCGCAACAAACGGGGCGAACGTTCTGACGATCGGCACAAAACGGGCGAGAATAATCGTTTTGCCGCCATGTTTCTCATAAAACTGATGGGTTTTGTCGAGATAACTGCGACGGAAAATTTTCGAATTGGGATTACTGAACAACTTTTCACCGAACAACCGCCCGATAGTGTAATTGACCGCGTCACCCACAATCGCGGCGATCAGCATCAGCACCACCATCATATGGACGTTGAGATCGTTGGTTTCCAGTGATG  >  NC_000913/2434112‑2434557

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑