Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,395,340	G→A	I2I (ATC→ATT)	nuoK ←	NADH:quinone oxidoreductase subunit K

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,395,340	0	G	A	100.0%	69.8 / NA	24	I2I (ATC→ATT)	nuoK	NADH:quinone oxidoreductase subunit K
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (14/10);  total (14/10)

TTCTGCCGCCGCGAGGCTGATGGCGAGAATGTACATCACCTGACCGTCGGTCTGGCCCCAGTAGCTTCCGGCGACCACGAAGGCCAGCGCGGAGGCGTTAATCATGATTTCCAGACCAATCAACATAAACAGCAGATTGCGACGGATAACCAGACCGGTTAAGCCAAGAACGAATAAGATTGCCGCGAGGATCAGTCCATGTTGTAAGGGGATCATGCGTGCTCCTCCGTTTTTCTTTTC‑GCGCTGTCGTCTTTACGATTGCTCAGCACTTCACCCGCACGCTCTTCACGACCGACGTGGAAGGCCACAACCAGACCTGCGAGCAGCAGCATAGAAGCCAGTTCCACCGCCAGTACGTAAGGCCCGAACAGCGTAATACCCACTGCTTTAGCACTGATTGGCGTACCGTCGATACCCTGATCGTTAACACCGAGGATGGCGTAAAC  >  NC_000913/2395130‑2395575                                                                                                                                                                                                                   |                                                                                                                                                                                                                                             ttCTGCCGCCGCGAGGCTGATGGCGAGAATGTCCATCACCTGACCGTCGGTCTGGCCCAAGTAGCTTCCGGCGAGCACGAAGGCCAGCGCGGAGGCGTTAATCATGATTTCCAGACCAATCAACATAAGCAGAAGATTGCGACGGATAACCAACCCGGTTAAGACAAGAACGAATAAGATTGCCGCGAGGATCAGTCCATGTTGTAAGGGAATCATGCGTGCTCCTCCGTTTTTCTTTTC‑GCGCTgtcgtt                                                                                                                                                                                                     >  2:228615/1‑250 (MQ=255) ttCTGCCGCCGCGAGGCTGATGGCGAGAATGTACATCACCTGACCGTCGGTCTGGCCCCAGTAGCTTCCGGCGACCACGAAGGCCAGCGCGGAGGCGTTAATCATGATTTCCAGACCAATCAACATAAACAGCAGATTGCGACGGATAACCAGACCGGTTAAGCCAAGAACGAATAAGATTGCCGCGAGGATCAGTCCATGTTGTAAGGGAATCATGCGTGCTCCTCCGTTTTTCTTTTC‑GCGCTgtcgtc                                                                                                                                                                                                     >  1:76039/1‑251 (MQ=255)       cgccgcGAGGCTGATGGCGAGAATGTACATCACCTGACCGTCGGTCTGGCCCCAGTAGCTTCCGGCGACCACGAAGGCCAGCGCGGAGGCGTTAATCATGATTTCCAGACCAATCAACATAAACAGCAGATTGCGACGGATAACCAGACCGGTTAAGCCAAGAACGAATAAGATTGCCGCGAGGATCAGTCCATGTTGTAAGGGAATCATGCGTGCTCCTCCGTTTTTCTTTTC‑GCGCTGTCGTCTTTACg                                                                                                                                                                                               >  1:219249/1‑251 (MQ=255)                gCTGATGGCGAGAATGTACATCACCTGACCGTCGGTCTGGCCCAAGTAGATTCCGGCGACCACGAAGGCCAGCGCGGAGGCGTTAATCATCATTTCCAGACCACTCAAAATAAACAGAAGATTGCGACGGATAACCAGACCGGTTAAGCCAAGAACGAATAAGATTGCCGCGAGGATCAGTCCATGTTGTAAGGGAATCCTGCGTGCTCCTCCGTTTTTCTTTTA‑GCGCTGTCGTCTTTACGATTGCTc                                                                                                                                                                                        >  1:109778/1‑249 (MQ=255)                          agaATGTACATCACCTGGCCGTCGGTCTGGCCCCAGTAGCTTCCGGCGACCACGAAGGCCAGCGCGGAGGCGTTAATCATGATTTCCAGACCAATCAACATAAACAGCAGATTGCGACGGATAACCAGACCGGTTAAGCCAAGAACGAATAAGATTGCCGCGAGGATCAGTCCATGTTGTAAGGGAATCATGCGTGCTCCTCCGTTTTTCTTTTC‑GCGCTGTCGTCTTTACGATGGCTCAGTACTTCAccc                                                                                                                                                                            <  2:22868/251‑1 (MQ=255)                                tACATCACCTGACCGTCGGTATCGCACCAGTAGCTTCCGGCGACCACGAAGGCCAGCGCGGAGGCGTTAATCATGATTTCCAGACCAATCAACATAAACAGCAGATTGCGACGGATAACCAGACCGGTTAAGCCCAGACCGAATAAGATTGCCGCGAGGATCAGTCCATGTTGAAAGGGAATCATGCGTGATCCTCCGTTTTTCTTTTC‑GCGCTGTCGTCTTTACGATTGCTCAGCCCTTCACCCGCACGc                                                                                                                                                                      >  2:118803/1‑251 (MQ=255)                                           aCCGTCGGTCTGGCCCCAGTAGCTTCCGGCGACCACGAAGGCCAGCGCGGAGGCGTTAATCATGATTTACAGACCAATCAACATAAACAGCAGATTGCGACGGATAACCAGACCGGTTAAGCCAAGAACGAATAAGATTGCCGCGAGGATCAGTCCATGTTGTAAGGGAATCATGCGTGCTCCTCCGTTTTTCTTTTC‑GCGCTGTCGTCTTTACGATTGCTCAGCAATTCACCCGCACGCTCTTCAcgacc                                                                                                                                                           >  1:198125/1‑251 (MQ=255)                                                  gTCTGGCAACAGTAGCTTCCGGCGACCACGAAGGCCAGCGCGGAGGCGTTAATCATGATTTCCAGACCAATCAACATAAACAGCAGATTGCGACGGATAACCAGACCGGTTAAGCCAAGAAAGAATAAGATTGCCGCGAGGATCAGTCCATGTTGTAAGGGAATCATGCGTGCTCCTCCGTTTTTCTTTTC‑GCGCTGTCGTCTTTACGATTGCTCAGCACTTCACCCGCACGCTCTTCACGACCGACGTgg                                                                                                                                                    >  2:186818/1‑251 (MQ=255)                                                        ccccAGTAGCTTCCGGCGACCACGAAGGCCCGCGCGGAGGCGTTAATCATGATTTCCAGACCAATCAACATAAACAGCAGATTGCGACGGATAACCAGACCGGTTAAGCCAAGAACGAATAAGATTGCCGCGAGGCTCAGTCCATGTTGTAAGGGAATCATGCGTGCTCCTCCGTTTTTCTTTTC‑GCGCTGTCGTCTTTACGATTGCTCAGCCCTTCATCCGCACGCTCTTCACGACCGACGTGGAAGGcc                                                                                                                                              >  2:85760/1‑251 (MQ=255)                                                                gCTTCCGGCGACCACGAAGGCCAGCGCGGAGGCGTTAATCATGATTTCCAGACCAATCAACATAAACAGCAGATTGCGACGGATAACCAGACCGGTTAAGCCAAGAACGAATAAGATTGCCGCGAGGATCAGTCCATGTTGTAAGGGAATCATGCGTGCTCCTCCGTTTTTCTTTTC‑GCGCTGTCGTCTTTACGATTGCTCAGCACTTCACCCGCACGCTCTTCACGACCGACGTGGAAGGCCACAAcc                                                                                                                                        >  2:14986/1‑249 (MQ=255)                                                                    ccGGCGACCACGAAGGCCAGCGCGGAGGCGTTAATCATGATTTCCAGACCAATCAACATAAACAGCAGATTGCGACGGATAACCAGACCGGTTAAGCCAAGAACGAATAAGATTGCCGCGAGGATCAGTCCATGTTGTAAGGGAATCATGCGTGCTCCTCCGTTTTTCTTTTC‑GCGCTGTCGTCTTTACGATTGCTCAGCACTTCACCCGCACGCTCTTCACGACCGACGTGGAAGGCCACAACCAGACCt                                                                                                                                  <  2:76039/251‑1 (MQ=255)                                                                    ccGGCGACCACGAAGGCCAGCGCGGAGGCGTTAATCATGATTTCCAGACCAATCAACAAAAACAGCAGATTGCGACGGATAACCAGACCGGTTAAGCCAAGAACGAATAAGATTGCCGCGAGGATCAGTCCATGTTGTAAGGGAATCATGCGTGCTCCTCCGTTTTTCTTTTC‑GCGCTGTCGTCTTTACGATTGCTCAGCACTTCACCCGCACGCTCTTCACGACCGACGGGGAAGGCCACAACCAGAcc                                                                                                                                   <  1:94068/250‑1 (MQ=255)                                                                        cGACCACGAAGGCCAGCGCGGAGGCGTTAATCATGATTTCCAGACCAATCAACATAAACAGCAGATTGCGACGGATAACCAGACCGGTTAAGCCAAGAACGAATAAGATTGCCGCGAGGATCAGTCCATGTTGTAAGGGAATCATGCGTGCTCCTCCGTTTTTCTTTTC‑GCGCTGTCGTCTTTACGATTGCTCAGCACTTCACCCGCACGCTCTTCACGACCGACGTGGAAGGCCACAACCAGACCTGCGa                                                                                                                              <  1:6165/251‑1 (MQ=255)                                                                                          cgGAGGCGTTAATCATGATTTCCAGACCAATCAACATAAACAGCAGATTGCGACGGATAACCAGACCGGTTAAGCCAAGAACGAATAAGATTGCCGCGAGGATCAGTCCATGTTGTAAGGGAATCATGCGTGCTCCTCCGTTTTTCTTTTC‑GCGCTGTCGTCTTTACGATTGCTCAGCACTTCACCCGCACGCTCTTCACGACCGACGTGGAAGGCCACAACCAGACCTGCGAGCAGCAGCATAGAAGcc                                                                                                             >  1:159680/1‑250 (MQ=255)                                                                                            gAGGCGTTAATCATGATTTCCAGACCAATCAACATAAACAGCAGATTGCGACGGATAACCAGACCGGTTAAGCCAAGAACGAATAAGATTGCCGCGAGGATCAGTCCATGTTGTAAGGGAATCATGCGTGCTCCTCCGTTTTTCTTTTC‑GCGCTGTCGTCTTTACGATTGCTCAGCACTTCACCCGCACGCTCTTCACGACCGACGTGGAAGGCCACAACCAGACCTGCGAGCAGCAGCATAGAAGCCAGt                                                                                                          >  2:195017/1‑251 (MQ=255)                                                                                                 gTTAATCATGATTTCCAGACCAATCAACATAAACAGCAGATTGCGACGGATAACCAGACCGGTTAAGCCAAGAACGAATAAGATTGCCGCGAGGATCAGTCCATGTTGTAAGGGAATCATGCGTGCTCCTCCGTTTTTCTTTTC‑GCGCTGTCGTCTTTACGATTGCTCAGCACTTCACCCGCACGCTCTTCACGACCGACGTGGAAGGCCACAACCAGACCTGCGAGCAGCAGCATAGAAGCCAGTTCCAc                                                                                                     <  1:111196/251‑1 (MQ=255)                                                                                                       cATGATTTCCAGACCAATCAACATAAACAGCAGATTGCGACGGATAACCAGACCGGTTAAGCCAAGAACGAATAAGATTGCCGCGAGGATCAGTCCATGTTGTAAGGGAATCATGCGTGCTCCTCCGTTTTTCTTTTC‑GCGCTGTCGTCTTTACGATTGCTCAGCACTTCACCCGCACGCTCTTCACGACCGACGTGGAAGGCCACAACCAGACCTGCGAGCAGCAGCATAGAAGCCAGTTCCACCGCCAg                                                                                               <  1:226022/251‑1 (MQ=255)                                                                                                                 aGACCAATCAACATAAACAGCAGATTGCGACGGATAACCAGACCGGTTAAGCCAAGAACGAATAAGATTGCCGCGAGGATCAGTCCATGTTGTAAGGGAATCATGCGTGCTCCTCCGTTTTTCTTTTC‑GCGCTGTCGTCTTTACGATTGCTCAGCACTTCACCCGCACGCTCTTCACGACCGACGTGGACGGCCACAACCAGACCTGCGAGCAGCAGCATAGAAGCCAGTTCCACCGCCAGTACGTAAGGc                                                                                     >  2:56156/1‑251 (MQ=255)                                                                                                                                    gcagATTGCGACGGATAACCAGACCGGTTAAGCCAAGAACGAATAAGATTGCCGCGAGGATCAGTCCATGTTGTAAGGGAATCATGCGTGCTCCTCCGTTTTTCTTTTC‑GCGCTGTCGTCTTTACGATTGCTCAGCACTTCACCCGCACGCTCTTCACGACCGACGTGGAAGGCCACAACCATACCTGCGAGCAGCAGCATAGAAGCCAGTTCCACCGCCAGTACGTAAGGCCCGAACAGCGTAATAccc                                                                   >  1:215222/1‑250 (MQ=255)                                                                                                                                               cGGATAACCAGCCCGGTTAAGCAAAGAACGAATAAGTTGGCCGCGAGGTTCATCCCATTTTTTAAGGGATTCATTCGTGCTCC‑CCCTTTTTCTTTTCCGGGCTGTCGTCTTTACGATGGCCCAGCACTCCCCCCGCCCGCCCTTCGCGACCGACGTGGAAGACCAAACCCAGCCCTGCAAGCAGCAGCAAAGAAGCCAGTTCCACCGCCAGTACGTAAGGCCCGAACAGCGTAATACCCACTGCTTTAGCa                                                       <  2:86791/251‑1 (MQ=255)                                                                                                                                                                     aaaaaaCGAATAAGATTGCCGGGAGGATCAGTCCATGTTGTAAGGGAATCAGGGTTGCTCCTCCGTTTTTCTTTTC‑GCCCGGTCGTCTTTACGATTGCTTACCACTTCCCCCGCCCGCTCTGCACGCCCGAGGTGGAAGGCCATAACCAGACCTGCGAGCAGCGGCAGAGAAGCCAGTTCCACCGCCAGTACGTAAGGCCCGAACAGCGTAATACCCACTGCTTTAGCACTGATTGGCGTACCGTCGATAc                                 <  1:133636/247‑1 (MQ=255)                                                                                                                                                                            aaaaaagttgcccgggtggttCAGTCGATGTTGTAAGGAAAGCATGCGGGCCCCCCCTTTTTTCTTTTC‑CCGCTGTGGTCTTTAGGATTGCCCGCCCCTCCCCCCGCACCCTCTTCACGCCCAACGTAAAAGCCCCCAACCAGACCTGCGACCATCACCATAGAGGCCGGTTCGCCCGCCAGTACGTAAGGCCCGAACAGCGTAATACCCACTGCTTTAGCACTGATTGGCGTACCGTCGATACCCTGATc                          <  1:121462/231‑1 (MQ=255)                                                                                                                                                                                           gAGGATCAGTCCATGTTGTAAGGGAATCATGCGTGCTCCTCCGTTTTTCTTTTC‑GCGCTGTCGTCTTTACGATTGCTCAGCACTTCACCCGCACGCTCTTCACGACCGACGTGGAAGGCCACAACCAGACCTGCGAGCAGCAGCATAGAAGCCAGTTCCACCGCCAGTACGTAAGGCCCGAACAGCGTAATACCCACTGCTTTAGCACTGATTGGCGTACCGTCGATACCCTGATCGTTAACACCGAGGAt           >  1:50623/1‑251 (MQ=255)                                                                                                                                                                                                    cccATGTTGTAAGGGACTCATGGGTGCTCCTCCGTTTTTCTTTCC‑GCGCTGTCGTCTTGCCGAGTGCACAGCACTCCCCCCGCACGCTCTTCACGACCGACGTGAAAGGCCACACCCAGACCTGCGAGCAGCAGCATAGAAGCCAGTCCCACCGCCAGTACGTAAGGCCCGAACAGCGTAATACCCACTGCTTTAGCACTGATTGGCGTACCGTCGATACCCTGATCGTTAACACCGAGGATGGCGTAAAc  <  1:142426/250‑1 (MQ=255)                                                                                                                                                                                                                   |                                                                                                                                                                                                                                             TTCTGCCGCCGCGAGGCTGATGGCGAGAATGTACATCACCTGACCGTCGGTCTGGCCCCAGTAGCTTCCGGCGACCACGAAGGCCAGCGCGGAGGCGTTAATCATGATTTCCAGACCAATCAACATAAACAGCAGATTGCGACGGATAACCAGACCGGTTAAGCCAAGAACGAATAAGATTGCCGCGAGGATCAGTCCATGTTGTAAGGGGATCATGCGTGCTCCTCCGTTTTTCTTTTC‑GCGCTGTCGTCTTTACGATTGCTCAGCACTTCACCCGCACGCTCTTCACGACCGACGTGGAAGGCCACAACCAGACCTGCGAGCAGCAGCATAGAAGCCAGTTCCACCGCCAGTACGTAAGGCCCGAACAGCGTAATACCCACTGCTTTAGCACTGATTGGCGTACCGTCGATACCCTGATCGTTAACACCGAGGATGGCGTAAAC  >  NC_000913/2395130‑2395575

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑