Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	239,359	C→T	intergenic (‑623/+60)	ykfM ← / ← yafV	uncharacterized protein YkfM/2‑oxoglutaramate amidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	239,359	0	C	T	100.0%	89.6 / NA	28	intergenic (‑623/+60)	ykfM/yafV	uncharacterized protein YkfM/2‑oxoglutaramate amidase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (18/10);  total (18/10)

ACAGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGCACAGGCGTCTGGACGTGGTAATGAATGAAGACGACTACAAAATAAGAAGAGGAAACGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTTGACGAATGAGAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAGTCCTTGCGGGGAGCGGGCTTTCGTAGTAA‑TACCCCGACTCTCCCCGT‑CCTTAAACACAACCCCCACTCACCA‑CAACCTAAACTCATCCGCATCCTGCCATGCCGGAAACTTTTCTCTATATTCCCGCAATGCTGCCATCGACAGCTCCGCATCAATGCGCGTTGCCTGATGCGCGTCGGCAGTAGCGATAATCTCTCCTTGCGGATTAATCACCCGGCTGTCACCGCGATAATGGCAGCCGTT  >  NC_000913/239130‑239595                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                acaGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGCACAGGCGTCTGGACGTGGTAATGAATGAAGACGACTACAACATAAGAAGAGGAAACGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTTGACGAATGAGAAGGAATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAGTCCTTGTGGTGAGCGGGCTTTCgtagt                                                                                                                                                                                                                             >  1:133888/1‑250 (MQ=255)     cAATCCGAAACCACTGGCACGTGGAGAATAAGCTGCACAGGCGTCTGGACGTGGTAATGAATGAAGACGACTACAAAATAAGAAGAGGAAACGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTTGACGAATGAGAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAGTCCTTGTGGGGAGCGGGCTTTCGTAGTAA‑TAc                                                                                                                                                                                                                       >  2:110880/1‑251 (MQ=255)                                                        tGGTAATGAATGAAGACGACTACAAAATAAGAAGAGGAAACGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTTGACGAATGAGAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAGTCCTTGTGGGGAGCGGGCTTTCGTAGTAA‑TACCCCGACTCTCCCCGT‑CCTTAAACACAATCCCCACTCACCA‑CAACCTAAACt                                                                                                                                                                  >  1:36621/1‑251 (MQ=255)                                                          gTAATGAATGACGCCGAATACAAAATAAGTAGAGGAAACGCAGCAGCATTATTTTCAGGGATAAGGCACATTGCTATTAATCTTTTGACGAATGAGAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAGTCCTTGTGGGGAGCGGGCTTTCGTAGTAA‑TACCCCGACTCTCCCCGT‑CCTTAAACACAATCACCACTCACCA‑CAACCTAAtctcc                                                                                                                                                                >  2:35438/1‑250 (MQ=18)                                                               gaatgaaGACGACTACAAAATAAGAAGAGGAAACGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTTGACGAATGAGAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAGTCCTTGTGGGGAGCGGGCTTTCGTAGTAA‑TACCCCGACTCTCCCCGT‑CCTTAAACACAATCCCCACTCACCA‑CAACCTAAATTCATCCGc                                                                                                                                                           >  1:224397/1‑251 (MQ=255)                                                                         gacTACAAAATAAGAAGAGGAAACGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTTGACGAATGAGAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAGTCCTTGTGGGGAGCGG‑CCTTCGGAGTAAATACCC‑GGCTCTCCCCGTTCTTTAAACACA‑TCCCCACTCCACA‑CCACCTAAAATCCACCCCATCCTGcccat                                                                                                                                                >  2:101000/1‑248 (MQ=14)                                                                                 aaTAAGAAGAGGAAACGCAGCAGAATTATTTTCAGGGATAAGGCACATTGCTATTAATATTTTGACGAATGAGAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAGTCCTTGTGGGGAGCGGGCTTTCGTAGTAA‑TACCCCGACTCTCCCCGT‑CCTTAAACACAATCCCCACTCACCA‑CAACCTAAACTCATCCGCATCCTGCCATGCCGGaa                                                                                                                                          >  1:230781/1‑250 (MQ=255)                                                                                             aaaCGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTTGACGAATGAGAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAGTCCTTGTGGGGAGCGGGCTTTCGTAGTAA‑TACCCCGACTCTCCCCGT‑CCTTAAACACAATCCCCACTCACCA‑CAACCTAAACTCATCCGCATCCTGCCATGCCGGAAACTTTTCTCtata                                                                                                                             <  1:111944/251‑1 (MQ=255)                                                                                             aaaCGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTTGACGAATGAGAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAGTCCTTGTGGGGAGCGGGCTTTCGTAGTAA‑TACCCCGACTCTCCCCGT‑CCTTAAACACAATCCCCACTCACCA‑CAACCTAAACTCATCCGCATCCTGCCATGCCGGAAACTTTTCTCtat                                                                                                                              >  2:125476/1‑250 (MQ=255)                                                                                                cGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTTGACGAATGAGAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAGTCCTTGTGGGGAGCGGGCTTTCGTAGTAA‑TACCCCGACTCTCCCCGT‑CCTTAAACACAATCCCCACTCACCA‑CAACCTAAACTCATCCGCATCCTGCCATGCCGGAAACTTTTCTCTATATTc                                                                                                                          <  1:73544/251‑1 (MQ=255)                                                                                                                   gggATACGGCACATTGCTATTAATATTTTGACGAATGAGAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAGTCCTTGTGGGGAGCGGGCTTTCGTAGTAA‑TACCCCGACTCTCCCCGT‑CCTTAAACACAATCCCCACTCACCA‑CAACCTAAACTCATCCGCATCCTGCCATGCCGGAAACTTTTCTCTATATTCCCGCAATGCTGCCATCGAc                                                                                                       <  1:181531/251‑1 (MQ=255)                                                                                                                                    tATTAATATTTTGACGAATGAGAAGGTATTCACGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAGTCCTTGTGGGGAGCGGGCTTTCGTAGTAA‑TACCCCGACTCTCCCCGT‑CCTTAAACACAATCCCCACTCACCA‑CAACATAAACTCATCCGCATCCTGCCATGCCGGCAACTTTTCTCTATATTTCCGCAATGCTGCCATCGACAGCTCCGCATCAATgcg                                                                                      >  2:2413/1‑251 (MQ=255)                                                                                                                                           atTTTGACGAATGAGAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAGTCCTTGTGGGGAGCGGGCTTTCGTAGTAA‑TACCCCGACTCTCCCCGT‑CCTTAAACACAATCCCCACTCACCA‑CAACCTAAACTCATCCGCATCCTGCCATGCCGGAAACTTTTCTCTATATTCCCGCAATGCTGCCATCGACAGCTCCGCATCAATGCGCGTTGcc                                                                               >  1:25756/1‑251 (MQ=255)                                                                                                                                              ttGACGAATGAGAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAGTCCTTGTGGGGAGCGGGCTTTCGTAGTAA‑TACCCCGACTCTCCCCGT‑CCTTAAACACAATCCCCACTCACCA‑CAACCTAAACTCATCCGCATCCTGCCATGCCGGAAACTTTTCTCTATATTCCCGCAATGCTGCCATCGACAGCTCCGCATCAATGCGCGTTGCCTg                                                                             <  1:110880/250‑1 (MQ=255)                                                                                                                                                 aCGAATGAGAAGGTATTCAAGTCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAGTCCTTGTGGGGAGCGGGCTTTCGTAGTAA‑TACCCCGACTCTCCCCGT‑CCTTAAACACAATCCCCACTCACCA‑CAACCTAAACTCATCCGCATCCTGCCATGCCGGAAACTTTTCTCTATATCCCCGCAATGCTGCCATCGACAGCTCCGCATCAATGCGCGTTGCCTGATgc                                                                         <  2:44756/251‑1 (MQ=255)                                                                                                                                                 aCGAATGAGAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAGTCCTTGTGGGGAGCGGGCTTTCGTAGTAA‑TACCCCGACTCTCCCCGT‑CCTTAAACACAATCCCCACTCACCA‑CAACCTAAACTCATCCGCATCCTGCCATGCCGGAAACTTTTCTCTATATTCCCGCAATGCTGCCATCGACAGCTCCGCATCAATGCGCGTTGCCTGATg                                                                          >  2:184053/1‑250 (MQ=255)                                                                                                                                                  cGAATGAGAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGCAAAGCAGCCATGGACAGAAACTACCTGGAGTCAGTCCTTGTGGGGAGCGGGCTTTAGTAGTAA‑TACCCCGACTCTCCCCGT‑CCTTAAACACAATCCCCACTCACCA‑CAACCTAAACTCATCCGCATCCTGCCATGCCGGAAACTTTTCTCTATATTCCCGCAATGCTGCCATCGACAGCTCAGCATCAATGCGCGCTGCCTGATgcg                                                                        >  1:241335/1‑251 (MQ=38)                                                                                                                                                      tGAGAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCACTGGACAGAAACTACCTGGCGTCCGTCCTTGTGGGGAGCGGGCTTTCGTAGTAA‑TACCCCGACTCTCCCCGT‑CCTTAAACACAATCCCCACTCACCA‑CAACCTAAACTCATCCGCATCCTGCCATGCCGGAAACTTTTCTCTATATTCCCGCACTGCTGCCATCGACAGCTCCGCATCAATGCGCGTTGCCTGATGCGCGTc                                                                    >  1:251600/1‑251 (MQ=255)                                                                                                                                                             gTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAGTCCTTGTGGGGAGCGGGCTTTCGTAGTAA‑TACCCCGACTCTCCCCGT‑CCTTAAACACAATCCCCACTCACCA‑CAACCTAAACTCATCCGCATCCTGCCATGCCGGAAACTTTTCTCTATATTccc                                                                                                                        >  1:24561/1‑192 (MQ=255)                                                                                                                                                             gTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAGTCCTTGTGGGGAGCGGGCTTTCGTAGTAA‑TACCCCGACTCTCCCCGT‑CCTTAAACACAATCCCCACTCACCA‑CAACCTAAACTCATCCGCATCCTGCCATGCCGGAAACTTTTCTCTATATTccc                                                                                                                        <  2:24561/192‑1 (MQ=255)                                                                                                                                                                  cAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAGTCCTTGTGGGGAGCGGGCTTTCGTAGTAA‑TACCCCGACTCTCCCCGT‑CCTTAAACACAATCCCCACTCACCA‑CAACCTAAACTCATCCGCATCCTGCCATGCCGGAAACTTTTCTCTATATTCCCGCAATGCTGCCATCGACAGCTCCGCATCAATGCGCGTTGCCTGATGCGCGTCGGCAGTAGCGa                                                         <  1:92731/250‑1 (MQ=255)                                                                                                                                                                              aaGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAGTCCTTGTGGGGAGCGGGCTTTCGTAGTAA‑TACCCCGACTCTCCCCGT‑CCTTAAACACAATCCCCACTCACCA‑CAACCTAAACTCATCCGCATCCTGCCATGCCGGAAACTTTTCTCTATATTCCCGCAATGCTGCCATCGACAGCTCCGCATCAATGCGCGTTGCCTGATGCGCGTCGGCAGTAGCGATAATCTCTCCTTg                                            <  1:19815/251‑1 (MQ=255)                                                                                                                                                                                   gTAAGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAGTACTTGTGGGGAGCGGGCTTTCGTAGTAA‑TACAACGACTCTCCCCGT‑CCTTAAACACAATCCCCACTCACCA‑CAACCTAAACTCATCCGCATCCTGCCATGCCGGAAACTTTTCTCTCTATTCCCGCAATGCTGCCATCGACAGCTCCGCATCAATGCGCGTTGCCTGATGCGCGTCGGCAGTAGCGATAATCTCTCCTTGCGGAt                                       >  1:112049/1‑251 (MQ=255)                                                                                                                                                                                              aaggcagcaATGGACAGAACCTCCCTGGCGGCAGTCCTTGTGGGGACCGGGCGTCCGAAGTAA‑TACCCTGACTCTCCCCGT‑CCTTAACCACAACCCCCCCTC‑CCATCAACCTAACCTCACCGGCATCCTGCCATGCCGGAAACTTGTCTCTATATTCCCGCAAGGTTGCCACGGCCAGCTCCGCATCAATGCGCGTTGCCTGATGCGCGTCGGCAGTAGGTATA‑TCTCGCCTTGCGGATTAATCTCCCGGc                           <  1:180937/248‑1 (MQ=255)                                                                                                                                                                                              aaaGCAGCCATGGACAGAAACTACCTGGCGTCAGTCCTTGTGGGGAGCGGGCTTTCGTAGTAA‑TACCCCGACTCTCCCCGT‑CCTTAAACACAATCCCCACTCGCCA‑CAACCTAAACTCATCCGCATCCTGCCATGCCGGAAACTTTTCTCTATATTCCCGCAATGCTGCCATCGACAGCTCCGCATCAATGCGCGTTGCCTGATGCGCGTCGGCAGTAGCGATAATCTCTCCTTGCGGATTAATCACCCgg                            >  1:34771/1‑251 (MQ=255)                                                                                                                                                                                                                    cccTGGCGTCAGGCCGTGTGGGGAGCGGGCTTTCGTAGGAA‑TCCCCCGACTCTCCCCGT‑CCTTAAACACAATCCCCCCTCACCA‑CAACCTAAACTCATCCGCATCCTGCCATGCCGGAAACTTTTCTCTATATTCCCGCAATGCTGCCATCGACAGCTCCGCATCAATGCGCGTTGCCTGATGCGCGTCGGCAGTAGCGATAATCTCTCCTTGCGGATTAATCACCCGGCTGTCACCGCGATAATGGCa        <  2:216934/248‑1 (MQ=255)                                                                                                                                                                                                                      cTGGCGTCAGTCCTTGTGGGGAGCGGGCTTTCGTAGTAA‑TACCCCGACTCTCCCCGT‑CCTTAAACACAATCCCCACTCACCA‑CAACCTAAACTCATCCGCATCCTGCCATGCCGGAAACTTTTCTCTATATTCCCGCAATGCTGCCATCGACAGCTCCGCATCAATGCGCGTTGCCTGATGCGCGTCGGCAGTAGCGATAATCTCTCCTTGCGGATTAATCACCCGGCTGTCACCGCGATAATGGCAGCCg    >  1:174000/1‑251 (MQ=255)                                                                                                                                                                                                                        ggCGTCAGTCCTTGTGGGGAGCGGGCTTTCGTAGTAA‑TACCCCGACTCTCCCCGT‑CCTTAAACACAATCCCCACTCACCA‑CAACCTAAACTCATCCGCATCCTGCCATGCCGGAAACTTTTCTCTATATTCCCGCAATGCTGCCATCGACAGCTCCGCATCAATGCGCGTTGCCTGATGCGCGTCGGCAGTAGCGATAATCTCTCCTTGCGGATTAATCACCCGGCTGTCACCGCGATAATGGCAGCCGtt  >  1:35796/1‑251 (MQ=255)                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                ACAGCAATCCGAAACCACTGGCACGTGGAGAATAAGCTGCACAGGCGTCTGGACGTGGTAATGAATGAAGACGACTACAAAATAAGAAGAGGAAACGCAGCAGAATTATTTTCAGGGATACGGCACATTGCTATTAATATTTTGACGAATGAGAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAGTCCTTGCGGGGAGCGGGCTTTCGTAGTAA‑TACCCCGACTCTCCCCGT‑CCTTAAACACAACCCCCACTCACCA‑CAACCTAAACTCATCCGCATCCTGCCATGCCGGAAACTTTTCTCTATATTCCCGCAATGCTGCCATCGACAGCTCCGCATCAATGCGCGTTGCCTGATGCGCGTCGGCAGTAGCGATAATCTCTCCTTGCGGATTAATCACCCGGCTGTCACCGCGATAATGGCAGCCGTT  >  NC_000913/239130‑239595

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑