BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,337,998	G→A	L475F (CTC→TTC)	gyrA ←	DNA gyrase subunit A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,337,998	0	G	A	96.3%	73.1 / ‑5.9	27	L475F (CTC→TTC)	gyrA	DNA gyrase subunit A
Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (18/8); minor base T (1/0); total (19/8)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.55e-01

AGAAAGCGGCTGATACTTAACGTAGCCCTGGTGAGAGAGCGTCACGACCACATCTTCCTGGGTGATCAGATCTTCCAGGTTGATGTCTGCGCTGTTGGCGGTGATTTCAGTACGACGTTTGTCACCGAACTGTTCACGAACCAGCTCCAGCTCTTCACGGATCACTTCCATCAGACGATCGGCGCTACCAAGAATACGCAACAGTTCCGCGATCTGATCCAGCAGCTCTTTGTATTCGTCGAGCAGTTTTTCGTGCTCAAGACCGGTCAGTTTCTGCAAACGCAGATCCAGAATCGCCTGAGCTTGCTGTTCGGTCAGGTAGTACAGACCATCACGCACGCCGAACTCTGGCTCCAGCCATTCCGGACGCGCAGCATCGTCGCCAGCACGTTCGAGCATCGCGGCAACGTTGCCCAGCTGCCACGGATTAGCAACCAGCGCAGTTTTCGCTTCTGCAGGCGTCGGCGCATGACGGATCAGTTCGAT  >  NC_000913/2337756‑2338241

aGAAAGCGGCTGATACTTAACGTAGCCCTGGTGAGAGAGCGTCACGACCACATCTTCCTGGGTGATCAGATCTTCCAGGTTGATGTCTGCGCTGTTGGCGGTGAGTTCAGTACGACGCTTGTCACCGAACTGTTCACGAACCAGCTCCAGCTCTTCACGGATCACTTCCATCCGACGATCGGCGCTACCAAGAATACGCAACAGTTCCGCGATCCGATCCAGCAGATCTTTGTATTCGTCGAACAGttttt                                                                                                                                                                                                                                             >  2:220294/1‑251 (MQ=255)

       ggCTGATACTTAACGTAGCCCTGGTGAGAGAGCGTCACGACCACATCTTCCTGGGTGATCAGATCTTCCAGGTTGATGTCTGCGCTGTTGGCGGTGATTTCAGTACGACGTTTGTCACCGAACTGTTCACGAACCAGCTCCAGCTCTTCACGGATCACTTCCATCAGACGATCGGCGCTACCAAGAATACGAAACAGTTCCGCGATCTGATCCAGCAGCTCTTTGTATTCGTCGAACAGTTTTTCGTgatc                                                                                                                                                                                                                                      >  1:138596/1‑248 (MQ=255)

         cTGATACTTAACGTAGCCCTGGTGAGAGAGCGTCACGACCACATCTTCCTGGGTGATCAGATCTTCCAGGTTGATGTCTGCGCTGTTGGCGGTGATTTCAGTACGACGTTTGTCACCGAACTGTTCACGAACCAGCTCCAGCTCTTCACGGATCACTTCCATCAGACGATCGGCGCTACCAAGAATACGCAACAGTTCCGCGATCTGATCCAGCAGCTCTTTGTATTCGTCGAACAGTTTTTCGTGCTCa                                                                                                                                                                                                                                     >  1:72283/1‑250 (MQ=255)

                    cGTAGCCCTGGTGAGAGAGCGTCCCGACCACATCTTCCTGGGTGATCAGATCTTCCAGGTTGATGTCTGCGCTGTTGGCGGTGATTTCAGTACGACGTTTGTCACCGAACTGTTCACGAACCAGCTCCAGCTCTTCACGGATCACTTCCATCAGACGATCGGCGCTACCAAGAATACGCAACAGTTCCGCGATCTGATCCAGCAGCTCTTTGTATTCGTCGAACAGTTTTTCGTGCTCAAGACCGGTCAGt                                                                                                                                                                                                                         <  1:262563/251‑1 (MQ=255)

                     gTAGCCCTGGTGAGAGAGCGTCACGACCACATCTTCCTGGGTGATCAGATCTTCCAGGTTGATGTCTGCGCTGTTGGCGGTGATTTCAGTACGACGTTTGTCACCGAACTGTTCACGAACCAGCTCCAGCTCTTCACGGATCACTTCCATCAGACGATCGGCGCTACCAAGAATACGCAACAGTTCCGCGATCTGATCCAGCAGCTCTTTGTATTCGTCGAACAGTTTTTCCTGCTCAAAACCGGTCAGtt                                                                                                                                                                                                                        >  2:260649/1‑251 (MQ=255)

                           cTGGTGAGAGAGCGTCACGACCACATCTTCCTGGGTGATCAGATCTTCCAGGTTGATGTCTGCGCTGTTGGCGGTGCTTTCAGTACGACGTTTGTCACCGAACTGTTCACGAACCAGCTCCAGCTCTTCACGGATCACTTCCATCAGACGATCGGCGCTACCAAGAATACGCAACAGTTCCGCGATCTGATCCAGCAGCTCTTTGTATTCGTCGAACCGTTTTTCGTGCTCAAGACCGGTCAGTTTCTGc                                                                                                                                                                                                                   >  1:168677/1‑250 (MQ=255)

                                                                aTCAGATCTTCCAGGTTGATGTCTGCGCTGTTGGCGGTGCTTTCCGTACGACGTTTGTCACCGAACTGTTCACGACACAGCTCAAGATCTTCACGGATCACTTCCATCAGACGATCGGCGCTACCAAGAATACGCAACAGTTCCGCGATCTGATCCCGAAGCTCTTTGTATTCGTAGATAAGTTTTTCGTGCTCACGACCGGTCAGTTTCTGCAAACGCAGATACAGAATCGCCTGAGCTTGCTGTTTGGt                                                                                                                                                                             >  1:13054/1‑251 (MQ=255)

                                                                   aGATCTTCCAGGTTGATGTCTGCGCTGTTGGCGGTGATTTCAGTACGACGTTTGTCACCGAACTGTTCACGAACCAGCTCCAGCTCTTCACGGATCACTTCCATCAGACGATCGGCGCTACCAAGAATACGCAACAGTTCCGCGATCTGATCCAGCAGCTCTTTGTATTCGTCGAACAGTTTTTCGTGCTCAAGACCGGTCAGTTTCTGCAAACGCAGATCCAGAATCGCCTGAGCTTGCTGTTCGGTc                                                                                                                                                                            >  1:217201/1‑249 (MQ=255)

                                                                                tGATGTCTGCGCTGTTGGCGGTGATTTCAGTACGACGTTTGTCACCGAACTGTTCACGAACCAGCTCCAGCTCTTCACGGATCACTTCCATCAGACGATCGGCGCTACCAAGAATACGCAACAGTTCCGCGATCTGATCCAGCCGCTCTTTGTATTCGTCGAACAGTTTTTCGTGCTCAAGACCGGGCAGTTTCTGCAAACGCAGATCCAGAATCGCCTGAGCTTGCTGTTCGGTCAGGTAGTACAGAcc                                                                                                                                                              >  1:43433/1‑250 (MQ=255)

                                                                                  aTGTCTGCGCTGTTGGCGGTGATTTCAGTACGACGTTTGTCACCGAACTGTTCACGAACCAGCTCCAGCTCTTCACGGATCACTTCCATCAGACGATCGGCGCTACCAAGAATACGCAACAGTTCCGCGATCTGATCCAGCAGCTCTTTGTATTCGTCGAACAGTTTTTCGTGCTCAAGACCGGTCAGTTTCTGCAAACGCAGATCCAGAATCGCCTGAGCTTGCTGTTCGGTCCGGTAGTACAGACCATc                                                                                                                                                           >  2:88463/1‑251 (MQ=255)

                                                                                        gcgcTGTTGGCGGTGATTTCAGTACGACGTTTGTCACCGAACTGTTCACGAACCAGCTCCAGCTCTTCACGGATCACTTCCATCAGACGATCGGCGCTACCAAGAATACGCAACAGTTCCGCGATCTGATCCAGCAGCTCTTTGTATTCGTCGAACAGTTTTTCGTGCTCAAGACCGGTCAGTTTCTGCAAACGCAGATCCAGAATCGCCTGAGCTTGCTGTTCGGTCAGGTAGTACAGACCATcacgc                                                                                                                                                       <  2:100819/249‑1 (MQ=255)

                                                                                        gcgcTGTTGGCGGTGATTTCAGTACGACGTTTGTCACCGAACTGTTCACGAACCAGCTCCAGCTCTTCACGGATAACTTCCATCAGACGCTCGGCGCTACCAAGAATACGCAACAGTTCCGCGATCTGATCCAGCAGATCTTTGTATTCGTCGAACAGTTTTTCGTGCTCAAGACCGGTCAGTTTCTGCAAACGCAGATCCAGAATCGCCTACGCTTGCTGTTCGGTCAGGTAGTACAGACCATcacgcac                                                                                                                                                     >  2:121722/1‑251 (MQ=255)

                                                                                                     tGATTTCAGAACGACGTTTGTCCCCGAAATGTTCACGAACCAGCTCCAGCTCTTCACGGATCACTTCCATCAGACGATCGGCGCTACCAAGAATACGCAACAGTTCCGCGATCTGATCCAGCAGCTCTTTGTATTCGTCGAACAGTTTTTCGTGCTCATGACCGGTCAGTTTCTGCAAACGCAGATCCAGAATCGCCTGAGCTTGCTGTTCGGTCAGGTAGTACAGCCCATCACGCACGCCGAACTCTGGc                                                                                                                                        >  2:120022/1‑251 (MQ=255)

                                                                                                         ttCAGTACGACGTTTGTCACCGAACTGTTCACGAACCAGCTCAAGCTCTTCACGGATCACTTCCATCAGACGATCGGCGCTACCAAGAATACGCAACAGTTCCGCGATCTGATCCAGCAGCTCTTTGTATTCGTCGAACAGTTTTTCGTGCTCAAGACCGGTCAGTTTCTGCAAACGCAGATCCAGAATCGCCTGAGCTTGCTGTTCGGTAAGGTAGTACAGACCATCACGCACGCCGAACTCTGGCTcc                                                                                                                                     >  2:34691/1‑250 (MQ=255)

                                                                                                                         tCACCGAACTGTTCACGAACCAGCTCCAGCTCTTCACGGATCACTTCCATCAGACGATCGGCGCTACCAAGAATACGCAACAGTTCCGCGATCTGATCCAGCAGCTCTTTGTATTCGTCGAACAGTTTTTCGTGCTCAAGACCGGTCAGTTTCTGCAAACGCAGATCCAGAATCGCCTGAGCTTGCTGTTCGGTCAGGTAGTACAGACCATCACGCACGCCGAACTCTGGCTCCAGCCATTCCGGACgcgc                                                                                                                    >  1:139270/1‑251 (MQ=255)

                                                                                                                          cACCGAACTGTTCACGAACCAGCTCCAGCTCTTCACGGATCACTTCCATCAGACGATCGGCGCTACCAAGAATACGCAACAGTTCCGCGATCTGATCCAGCAGCTCTTTATATTCGTCGCACAGTTTTTCGTGCTCAAGACCGGTCAGTTTCTGCAAACGCAGATCCAGAATCGCCTGAGCTTGCTGTTCGGTCAGGTAGTACAGACCATCACGCACGCCGACCTCTGGCTCCACCCATTCCCGAcgcac                                                                                                                    >  2:219135/1‑248 (MQ=255)

                                                                                                                                      cACGAACCAGCTCCAGCTCTTCACGGATCACTTCCATCAGACGATCGGCGCTACCAAGAATACGCAACAGTTCCTCGATCTGATCCAGCAGCTCTTTGTATTCGTCGAACAGTTTTTCGTGCTCAATACCGGTCAGTTTCTGCAAACGCAGATCCAGAATCGCCTGAGCGTGCTGTTCGGTCAGGTAGTACAGACCATCACGCACGCCGAACTCTGGCTCCAGCCATTCCGGACGCGCAGCATCGTCGCCa                                                                                                       <  1:135781/251‑1 (MQ=255)

                                                                                                                                       aCGAACCAGCTCCAGCTCTTCACGGATCACTTCCATCAGACGCTCGGCGCTACCCAGAATACGCAACCGTTCCGCGCTCTGATCCAGCAGCTCTTTGCATTCGTCGAACAGTTTTTCGTGCTCACGCCCGGTCAGTTTCTGCAAACGCAGATCCAGAATCGCCTGAACTTGCTGTTCGGTCCGGGAGTACAGACCATCCCGCACGCCGAACTCTGGCTCCAGCCCTTCCGGAAGCACAGCATCGTCGcc                                                                                                        >  2:242175/1‑249 (MQ=255)

                                                                                                                                        cgaactagaaccagctcTTCACGGAGCCCTTCCATCAGACGCTCGGCGCTACCAAGACTACGCACCAGTTCCGCGATATGATCCAGCAGCTCTTTGTCTTCGTCGCACAGTTTTTCGTGCTCAAAACCGGTCAGTTTATGCAAACGCAGATACAGAAACGCCTGAGCTTGCTGGTCGGTCAGGTAGTACAGACCATCACGCACGCAGAACACTGGCTCACGCCATTCCGGAAGAGAAGAATCGTCGCAAGc                                                                                                     >  2:149020/11‑251 (MQ=255)

                                                                                                                                                          gcACGGATCACTTCCATCAGACGATCGGCGCGACCAAGAATACGCAACAGTTCCGCGATCTGATCCAGCAGCTCTTTGTAGTCGTCGAACAGTTTTTCGTGCTCAAGACCGGTCAGTTTCTGCAAACGCAGATCCAGAATCGCCTGAGCTTGCTGTTCGGTCAGGTAGTACAGACCGTCACGCACGCCGAACTCTGGCTCCAGCCATTCCGGACGCGCAGCATCGTCGCCAGCACGTGCGAGCATcgcg                                                                                     <  2:132265/248‑1 (MQ=255)

                                                                                                                                                            aCGGATCACTTCCATCAGACGATCGGCGCTACCAAGAATACGCAACAGTTCCGCGATCTGATCCAGCAGCTCTTTGTATTCGTCGAACAGTTTTTCGTGCTCAAGACCGGTCAGTTTCTGCAAACGCAGATCCAGAATCGCCTGAGCTTGCTGTTCGGTCAGGTAGTACAGACCATCACGCACGCCGAACTCTGGCTCCAGCCATTCCGGACGCGCAGCATCGTCGCCAGCACGTTCGAGCATCGCGGCa                                                                                  >  2:116164/1‑250 (MQ=255)

                                                                                                                                                                             aaCGATCTGCGCTACCAAGAATACGCAACAGTTCCGCGATCTGATCCAGCAGCTCTTGGTATTCGTCGAACAGTTTTTCGTGCTCAAGACCGGTCAGTTTCTGCAAACGCAGATCCAGAATCGCCTGAGCTTGCTGTTCGGTCAGGTAGTACAGACCTTCACGCACGCCGAACTCTGGCTCCAGCCATGCCGGACGCGCAGCATCGTCGCCAGCACGTTCGAGCATCGCGGCAACGTTGCCCAGCTGCCAc                                                                <  2:168677/250‑1 (MQ=255)

                                                                                                                                                                                           ccAAGAATACGCAACAGTTCCGCGATCTGATCCAGCAGCTCTTTGTATTCGTCGAACAGTTTTTCGTGCTCAAGACCGGTCAGTTTCTGCAAACGCAGATCCAGAATCGCCTGAGCTTGCTGTTCGGTCAGGTAGTACAGACCATCACGCACGCCGAACTCTGGCTCCAGCCATTCCGGACGCGCAGCATCGTCGCCAGCACGTTCGAGCATCGCGGCAACGTTGCCCAGCTGCCACGGATTAGCAACCAg                                                  <  2:217201/251‑1 (MQ=255)

                                                                                                                                                                                                            ttCCGCGATCTGATCCAGCAGCTCTTTGTATTCGTCGAACAGTTTTTCGTGCTCAAGACCGGTCAGTTTCTGCAAACGCAGATCCAGAATCGCCTGAGCTTGCTGTTCGGTCAGGTAGTACAGACCATCACGCACGCCGAACTCTGGCTCCAGCCATTCCGGACGCGCAGCATCGTCGCCAGCACGTTCGAGCATCGCGGCAACGTTGCCCAGCTGCCACGGATTAGCAACAAGCGCAGTTTTCGCTTCTg                                 >  2:44672/1‑251 (MQ=255)

                                                                                                                                                                                                             tCCGCGATCTGATCCAGCAGCTCTTTGTATTCGTCGAACAGTTTTTCGTGCTCAAGACCGGTCAGTTTCTGCAAACGCAGATCCAGAATCGCCTGAGCTTGCTGTTCGGTCAGGTAGTACAGACCATCACGCACGCCGAACTCTGGCTCCAGCCATTCCGGACGCGCAGCATCGTCGCCAGCACGTTCGAGCATCGCGGCAACGTTGCCCAGCTGCCACGGATTAGCAACCAGCGCAGTTTTCGCTTCTg                                 <  1:214347/250‑1 (MQ=255)

                                                                                                                                                                                                                  gATCTGATCCAGCAGCTCTTTGTATTCGTCGAACAGTTTTTCGTGCTCAAGACCGGTCAGTTTCTGCAAACGCAGATCCAGAATCGCCTGAGCTTGCTGTTCGGTCAGGTAGTACAGACCATCACGCACGCCGAACTCTGGCTCCAGCCATTCCGGACGCGCAGCATCGTCGCCAGCACGTTCGAGCATCGCGGCAACGATGCCCAGCTGCCACGGATTAGCAACCAGCGCAGTATTCGCTTCTGCAGGCg                           >  1:206973/1‑251 (MQ=255)

                                                                                                                                                                                                                                            cgtcgAACAGTTTTTCGTGCTCAAGACCGGTCAGTTTCTGCAAACGCAGATCCAGAATCGCCTGAGCTTGCTGTTCGGTCAGGTAGTACAGACCATCACGCACGCCGAACTCTGGCTCCAGCCATTCCGGACGCGCAGCATCGTCGCCAGCACGTTCGAGCATCGCGGCAACGTTGCCCAGCTGCCACGGATTAGCAACCAGCGCAGTTTTCGCTTCTGCAGGCGTCGGCGCATGACGGATCAGTTCgat  <  1:184859/250‑1 (MQ=255)

AGAAAGCGGCTGATACTTAACGTAGCCCTGGTGAGAGAGCGTCACGACCACATCTTCCTGGGTGATCAGATCTTCCAGGTTGATGTCTGCGCTGTTGGCGGTGATTTCAGTACGACGTTTGTCACCGAACTGTTCACGAACCAGCTCCAGCTCTTCACGGATCACTTCCATCAGACGATCGGCGCTACCAAGAATACGCAACAGTTCCGCGATCTGATCCAGCAGCTCTTTGTATTCGTCGAGCAGTTTTTCGTGCTCAAGACCGGTCAGTTTCTGCAAACGCAGATCCAGAATCGCCTGAGCTTGCTGTTCGGTCAGGTAGTACAGACCATCACGCACGCCGAACTCTGGCTCCAGCCATTCCGGACGCGCAGCATCGTCGCCAGCACGTTCGAGCATCGCGGCAACGTTGCCCAGCTGCCACGGATTAGCAACCAGCGCAGTTTTCGCTTCTGCAGGCGTCGGCGCATGACGGATCAGTTCGAT  >  NC_000913/2337756‑2338241

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`