Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,333,124	G→A	intergenic (‑3327/+1212)	yfaQ ← / ← yfaT	tandem DUF2300 domain‑containing protein YfaQ/DUF1175 domain‑containing protein YfaT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,333,124	0	G	A	100.0%	76.5 / NA	24	intergenic (‑3327/+1212)	yfaQ/yfaT	tandem DUF2300 domain‑containing protein YfaQ/DUF1175 domain‑containing protein YfaT
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (15/9);  total (15/9)

GGCATAACGCAAATCGTTACCGACCATTGATAATTGCTGAGCGCGCAAACTTAACTGCACGCGGGCATTTTTTACCGGCTCGCCATCCGGGTAGAGCAGTTGCAGTTTGCCGCTGACCGCTTCGCCAGTTTTGAACTCTTTTTTGGCGAGAGCTAAACCAATCTCGAAATGTGGCTTGATGTAGTTTGCCACGCGAAAACTGCTGCTATAGACCTGATTGCGGTAAGCAAGACGTAACTCATAACCTCCGGCTACGGCATTTTCTGGCAGGCGGAAACTTCCCTGCCCGCCATTGCGCGCATCCAGCGTGACATTGACGGTTTGCAACAGACTGCCGTTGGCGTCCAGCACCGAAAGCTTCGCCGGGGCGCTGACGATGGGGGATGAATGCAACGGATCGTGGAACTCGCGGCCGATCACTTTAACATCGACACG  >  NC_000913/2332894‑2333328                                                                                                                                                                                                                                       |                                                                                                                                                                                                             ggCATAACGCAAATCGTTACCGACCATTGATAATTGCTGAGCGCGCAAACTTAACTGCACGCGGGCATTTTTTACCGGCTCGCCATCCGGGTAGAGCCGTTGCAGTTTGCCGCTGACCGCTTCGCCCGTTTTGAACTCTTTTTTGGCGAGAGCTAAACCAATCTCGAAATGTGGCTTGATGTAGTTTGCCACGCGAAAACTGCTGCTATAGCCCTGCTTGCGGTAAGCAAAACGTAACTCATAACCTCCgg                                                                                                                                                                                          >  2:148995/1‑251 (MQ=255)                     cGACCATTGATAATTGCTGAGCGCGCAAACTTAACTGCACGCGGGCATTTTTTACCGGCTCGCCATCCGGGTAGAGCAGTTGCAGTTTGCCGCTGACCGCTTCGCCAGTTTTGAACTCTTTTTTGGCGAGAGCTAAACCAATCTCGAAATGTGGCTTGATGTAGTTTGCCACGCGAAAACTGCTGCTATAGACCTGATTGCGGTAAGCAAAACGTAACTCATAACCTCCGGCTACGGCATTTTCTGGCAgg                                                                                                                                                                      >  1:229784/1‑251 (MQ=255)                       aCCATTGATAATTGCTGAGCGCGCAAACTTAACTGCACGCGGGCATTTTTTACCGGCTCGCCATCCGGGTAGAGCAGTTGCAGTTTGCCGCTGACCGCTTCGCCAGTTTTGAACTCTTTTTTGGCGAGAGCTAAACCAATCTCGAAATGTGGCTTGATGTAGTTTGCCACGCGAAAACTGCTGCTATAGACCTGATTGCGGTAAGCAAAACGTAACTCATAACCTCCGGCTACGGCATTTTCTGGCAGGCg                                                                                                                                                                    >  1:143292/1‑251 (MQ=255)                                    gCTGAGCGCGCAAACTTAACTGCACGCGGGCATTTTTTACCGGCTCGCCATCCGGGTAGAGCAGTTGCAGTTTGCCGCTGACCGCTTCGCCAGTTTTGAACTCTTTTTTGGCGAGAGCTAAACCAATCTCGAAATGTGGCTTGATGTAGTTTGCCACGCGAAAACTGCTGCTATAGACCTGATTGCGGTAAGCAAAACGTAACTCATAACCTCCGGCTACGGCATTTTCTGGCAGGCGGAAACTTCCCTGc                                                                                                                                                       <  1:255301/251‑1 (MQ=255)                                         gcgcgcAAACTTAACTGCACGCGGGCATTTTTTACCGGCTCGCCATCCGGGTAGAGCTGTTGCAGGTTGCCGCTGACCGCTTCGCCAGTTTTGAACTCTTTTTTGGCGAGAGCTAAACCAATCTCGAAATGTGGCTTGATGTAGTTTGCCACGCGAAAACTGCTGCTATAGACCTGATTGCGGTAAGCAAAACGTAACTCATAACCTCCGGCTACGGCATTTTCTGGCAGGCGGAAACTTCCCTGCCCGcc                                                                                                                                                  <  2:204185/251‑1 (MQ=255)                                                    tAACTGCACGCGGGCATTTTTTACCGGCTCGCCATCCGGGTAGAGCAGTTGCAGTTTGCCGCTGACCGCTTCGCCAGTTTTGAACTCTTTTTTGGCGAGAGCTAAACCAATCTCGAAATGTGGCTTGATGTAGTTTGCCACGCGAAAACTGCTGCTATAGACCTGATTGCGGTAAGCAAAACGTAACTCATAACCTCCGGCt                                                                                                                                                                                        <  2:202261/202‑1 (MQ=255)                                                    tAACTGCACGCGGGCATTTTTTACCGGCTCGCCATCCGGGTAGAGCAGTTGCAGTTTGCCGCTGACCGCTTCGCCAGTTTTGAACTCTTTTTTGGCGAGAGCTAAACCAATCTCGAAATGTGGCTTGATGTAGTTTGCCACGCGAAAACTGCTGCTATAGACCTGATTGCGGTAAGCAAAACGTAACTCATAACCTCCGGCt                                                                                                                                                                                        >  1:202261/1‑202 (MQ=255)                                                              cgGGCATTTTTTACCGGCGCGCCATCCGGGTAGAGCAGTTGCAGCTTCCCGCTGACCGCTTCGCCAGTTTTGAACTATTTTTTGGCGAGAGCTAAACCAATCTCGAAATGTGGCTTGATGTCGTTTGCCACGCGAAAAATGCTGCTATAGCCCTGATTGCGGTAAGCAAAACGTAACTCATAACCTCAGGCTACGGCATTTTCTGGCAGGCGGAACCATCCCTGCCCGCCATTGCGCGCATCCCGCGTGAc                                                                                                                             >  2:229372/1‑251 (MQ=255)                                                                       tttACCGGCTCGCCATCCGGGTAGAGCAGTTGCAGTTTGCCGCTGACCGCTTCGCCAGTTTTGAACTCTTTTTTGGCGAGAGCTAAACCAATCTCGAAATGTGGCTTGATGTAGTTTGCCACGCGAAAACTGCTGCTATAGACCTGATTGCGGTAAGCAAAACGTAACTCATAACCTCCGGCTACGGCATTTTCTGGCAGGCGGAAACTTCCCTGCCCGCCATTGCGCGCATCCAGCGTGACATTGACGGt                                                                                                                    >  1:62989/1‑251 (MQ=255)                                                                                  gCCATCCGGGTAGAGCAGTTGCAGTTTGCCGCTGACCGCTTCGCCAGTTTTGAACTCTTTTTTGGCGAGAGCTAAACCAATCTCGAAATGTGGCTTGATGTAGTTTGCCACGCGAAAACTGCTGCTATAGCACTGATTGCGGTAAGCAAAACGTAACTCATAACCTCCGGCTACGGCATTTTCTGGCAGGCGGAAACTTCCCTGCCCGCCATTGCGCGCATCCAGCGTGACATTGACGGTTTGCAACAGAc                                                                                                         >  2:91236/1‑251 (MQ=255)                                                                                    cATCCGGGTAGAGCAGTTGCAGTTTGCCGCTGACCGCTTCGCCAGTTTTGAACTCTTTTTTGGCGAGAGCTAACCCAATCTCGAAATGTGGCTTGATGTAGTTTGCCACGCGAAAACTGCTGCTATAGACCTGATTGCGGTAAGCAAAACGTAACTCATAACCTCCGGCTACGGCATTTTCTGGCAGGCGGAAACTTCCCTGCCCGCCATTGCGCGCATCCAGCGTGACATTGACGGTTTGCAACAGACTg                                                                                                       >  1:151975/1‑251 (MQ=255)                                                                                                        agttTGCCGCTGACCGCTTCGCCAGTTTTGAACTCTTTTTTGGCGAGAGCTAAACCAATCTCGAAATGTGGCTTGATGTAGTTTGCCACGCGAAAACTGCTGCTATAGACCTGATTGCGGTAAGCAAAACGTAACTCATAACCTCCGGCTACGGCATTTTCTGGCAGGCGGAAACTTCCCTGCCCGCCATTGCGCGCATCCAGCGTGACATTGACGGTTTGTAACAGACTGCCGTTGGCGTCCAGCACCg                                                                                    >  1:101150/1‑250 (MQ=255)                                                                                                           ttGCCGCTGACCGCTTCGCCAGTTTTGAACTCTTTTTTGGCGAGAGCTAAACCAATCTCGAAATGTGGCTTGATGTAGTTTGCCACGCGAAAACTGCTGCTATAGACCTGATTGCGGTAAGCAAAACGTAACTCATAACCTCCGGCTACGGCATTTTCTGGCAGGCGGAAACTTCCCTGCCCGCCATTGCGCGCATCCAGCGTGACATTGACGGTTTGCAACAGACTGCCGTTGGCGTCCAGCACCGac                                                                                  >  2:258770/1‑248 (MQ=255)                                                                                                            tgcggCTGACCGTTTCGCCATTTTTGAACTCTTTTTTGGCGAGGGCTGAACCAATCCCGAAATGTGGCTTGATGTAGTTTGCCCCGCGAAAACTGCTGCTATAGTCCGGATTGCGGTAAGCAAAACGTAACTCATAACCTCCGTCTACGGCATTTTCTGGCAGGCGGAAACTCCCCTGCCCGCCATTGCGCGCATCCAGCGTGACATTGACGGTTTGCAACAGACTGCCGTTGGCGTCCAGCGCCGAAAg                                                                                <  2:176407/246‑1 (MQ=255)                                                                                                                   gACCGCTTCGCAAGTTTTGCACTCTTTTTTGACGACAACTAAACAAATCTCGCCATGAGGCTTGATGTAGTTTGCCCCGAGCAAGCTGCTGCCATAGACCTGATTGCGGTAAGCACACCGTAACTCATAACATCCGGCTACGGAATTTTCTGGCAGGAGGAAACTTCCCTGCCCGCCATTGCGCGCATCCAGCGTGAAATTGACGGTTTGCAACAGACTGCCGTTGGCGTCCAGCACAGCAAGCTTCGCCg                                                                        >  2:154065/1‑251 (MQ=255)                                                                                                                                               ttGGCGAGAGCTAAACCAATCTCGAAATGTGGCTTGATGTAGTTTGCCACGCGAAAACTGCTGCTATAGACCTGATTGCGGTAAGCAAAACGTAACTCATAACCTCCGGCTACGGCATTTTCTGGCAGGCGGAAACTTCCCTGCCCGCCATTGCGCGCATCCAGCGTGACATTGACGGTTTGCAACAGACTGCCGTTGGCGTCCAGCACCGAAAGCTTCGCCGGGGCGCTGACGATGGGGGGTGAATGCa                                             >  2:136834/1‑250 (MQ=255)                                                                                                                                                          tAAACCAATCTCGAAATGTGGCTTGATGTAGTTTGCCACGCGAAAACTGCTGCTATAGACCTGATTGCGGTAAGCAAAACGTAACTCATAACCTCCGGCTACGGCATTTTCTGGCAGGCGGAAACTTCCCTGCCCGCCATTGCGCGCATCCAGCGTGACATTGACGGTTTGCAACAGACTGCCGTTGGCGTCCAGCACCGACAGCTTCGCCGGGGCGCTGCCGATGGGGGATGAATGCAACGGATCGTgg                                  >  1:133340/1‑250 (MQ=255)                                                                                                                                                            agccAATCTCGAAATGGGGTTTGAGTTAGTTTGCCAGGCGAAAACGGCTGCTAGAGCCCTGATTGCGGTAAGCAAAACGTAACTCATACCCTCCGGCTACGGCATTTTCTGGCAGGCGGAAACTTCCCTGCCCGCCATTGCGCGCATCCAGCGTGACATTGACGGTTTGCAACAGACTGCCGTTGGCGTCCAGCACCGAAAGCTTCGCCGGGGCGCTGACGATGGGGGATGAATGCAACGGATCGGGGAAc                               <  2:152453/249‑1 (MQ=255)                                                                                                                                                              ccAATCTCGAAATGTGGCTTGATGTAGTTTGCCACGCGAAAACTGCTGCTATAGACCTGATTGCGGTAAGCAAAACGTAACTCATAACCTCCGGCTACGGCATTTTCTGGCAGGCGGAAACTTCCCTGCCCGCCATTGCGCGCATCCAGCGTGACATTGACGGTTTGCAACAGACTGCCGTTGGCGTCCAGCACCGAAAGCTTCGCCGGGGCGCTGACGATGGGGGATGAATGCAACGGATCGTGGAACTc                             <  1:136834/251‑1 (MQ=255)                                                                                                                                                                        aaTGTGGCTTGATGGAGTTTGCCACGCGAAAACTGCTGCTATAGCCCTGATTGCGGGTAGCAAAACGTAACCCATAACCCCCGGCTCCGGCTTTTTCTGGCAGGCGGAACCTTCCCTGCCCGCCATTGCGCGCATCCAGCGTGACATTGACGGTTTGCAACAGACTGCCGTTGGCGTCCAGCACCGAAAGCTTCGCCGGGGCGCTGACGATGGGGGATGAATGCAACGGATCGTGGAACTCGCGGCCGATc                   <  1:193854/251‑1 (MQ=255)                                                                                                                                                                            gggCTTGATGTAGTTTGCCACGCGAAAACTGCTGCTATAGCCCTGATTGCGGTAAGCAAAACGTAACTCATAACCTCCGGCTACGGCATTTTCTGGCAGGCGGAAACTTCCCTGCCCGCCATTGCGCGCATCCAGCGTGACATTGACGGTTTGCAACAGACTGCCGTTGGCGTCCAGCACCGAAAGCTTCGCCGGGGCGCTGACGATGGGGGATGAATGCAACGGATCGTGGAACTCGCGGCCGATCACtt               <  2:129458/250‑1 (MQ=255)                                                                                                                                                                                         tttGCCACGCGAACACTGCTGCTATAGACCTGATTGCGGTAAGCAAAACGTAACTCATAACCTCCGGCTACGGCATTTTCTGGCAGGCGGAAACTTCCCTGCCCGCCATTGCGCGCATCCAGCGTGACATTGACGGTTTGCCACAGACTGCCGTTGGCGTCCAGCACCGAAAGCTTCGCCGGGGCGCTGACGATGGGGGATGAATGCAACGGATCGTGGAACTCGCGGCAGATCACTTTAACAGCGCCACg  >  2:178800/1‑251 (MQ=255)                                                                                                                                                                                                         tgctgcTATAGACCTGATTGCGGTAAGCAAAACGTAACTCATAACCTCCGGCTACGGCATTTTCTGGCAGGCGGAAACTTCCCTGCCCTCCATTGCGCGCATCCAGCGTGACATTGACGGTTTGCAACAGACTGCCGt                                                                                                   >  1:10757/1‑138 (MQ=255)                                                                                                                                                                                                         tgctgcTATAGACCTGATTGCGGTAAGCAAAACGAAACTCATAACCTCCGGCTACGGCATTTTCTGGCAGGCGGAAACTTCCCTGCCCGCCATTGCGCGCATCCAGCGTGACATTGACGGTTTACAACAGACTGCCGt                                                                                                   <  2:10757/138‑1 (MQ=255)                                                                                                                                                                                                                                       |                                                                                                                                                                                                             GGCATAACGCAAATCGTTACCGACCATTGATAATTGCTGAGCGCGCAAACTTAACTGCACGCGGGCATTTTTTACCGGCTCGCCATCCGGGTAGAGCAGTTGCAGTTTGCCGCTGACCGCTTCGCCAGTTTTGAACTCTTTTTTGGCGAGAGCTAAACCAATCTCGAAATGTGGCTTGATGTAGTTTGCCACGCGAAAACTGCTGCTATAGACCTGATTGCGGTAAGCAAGACGTAACTCATAACCTCCGGCTACGGCATTTTCTGGCAGGCGGAAACTTCCCTGCCCGCCATTGCGCGCATCCAGCGTGACATTGACGGTTTGCAACAGACTGCCGTTGGCGTCCAGCACCGAAAGCTTCGCCGGGGCGCTGACGATGGGGGATGAATGCAACGGATCGTGGAACTCGCGGCCGATCACTTTAACATCGACACG  >  NC_000913/2332894‑2333328

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑