Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,323,558	G→A	A38T (GCA→ACA)	atoD →	acetyl‑CoA:acetoacetyl‑CoA transferase subunit alpha

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,323,558	0	G	A	100.0%	30.1 / NA	12	A38T (GCA→ACA)	atoD	acetyl‑CoA:acetoacetyl‑CoA transferase subunit alpha
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (9/3);  total (9/3)

ATCCCCATCCAATTTATCCCTTCATATTCAATTAGTTAAATAACTAAATCCAATAATCTCATTCTGGCACTCCCCTTGCTATTGCCTGACTGTACCCACAACGGTGTATGCAAGAGGGATAAAAAATGAAAACAAAATTGATGACATTACAAGACGCCACCGGCTTCTTTCGTGACGGCATGACCATCATGGTGGGCGGATTTATGGGGATTGGCACTCCATCCCGCCTGGTTGAAGCATTACTGGAATCTGGTGTTCGCGACCTGACATTGATAGCCAATGATACCGCGTTTGTTGATACCGGCATCGGTCCGCTCATCGTCAATGGTCGAGTCCGCAAAGTGATTGCTTCACATATCGGCACCAACCCGGAAACAGGTCGGCGCATGATATCTGGTGAGATGGACGTCGTTCTGGTGCCGCAAGGTACGCTAATCGAGCAAATTCGCTGTGGTGGAGCTGGACTTGGTGGTTTTCTC  >  NC_000913/2323322‑2323800                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                   aTCCCCATCCAATTTATCCCTTCATATTCAATTAGTTAAAGAACTAAATCCAATAATCTCATTCTGGCACTCCCCTGGCTATTGCCTGACTGTACCCACAACGGTGTATGCAAGAGGGATAAAAAATGAAAACAAAATTGATGACATTACAAGACGCCCCCGGCTTCTGTCGTGACGGCATGACCATCATGGTGGGCGGATTTATGTGGATTGGCACTCCATCCCGCCTGGTTGAAACATTACTGGAATCt                                                                                                                                                                                                                                      <  1:247505/251‑1 (MQ=255)                                              aaaTCCAATAATCTCAGTCTGGCACTCCCCTGGCTATTGCCTGACGGTACCACCAACGGTGTATGCAAGAGGGATAAAAAATGAAAACAAAATTGATGACATTACAAGACGCCACCGGCTTCTTGCGTGACGGCATGACCAGCATGGTGGGCGGATTTATGGGGATTGGCACTCCATCCCGCCTGGTTGAAACATTACTGGAATCTGGTGTTCGCGACCTGACATTGATAGCCAATGATTCCGCGTttg                                                                                                                                                                                           <  2:126757/249‑1 (MQ=255)                                                         tctcATTCTGGCACTCCCCTTGCTATTGCCTGACTGTACCCACAACGGTGTATGCAAGAGGGATAAAAAATGAAAACAAAATTGATGACATTACAAGACGCCACCGGCTTCTTTCGTGACGGCATGACCATCATGGTGGGCGGATTTATGGGGATTGGCACTCCATCCCGCCTGGTTGAAACATTACTGGAATCTGGTGTTCGCGACCGCGCATTGATAGCCAATGATAACGCGTTTTTTGTTACCGGcct                                                                                                                                                                              >  2:193596/1‑249 (MQ=255)                                                              ttCTGGCACTCCCCTTGCTATTGCCTGACTGTACCCACAACGGTGTATGCAAGAGGGATAAAAAATGAAAACAAAATTGATGACATTACAAGACGCCACCGGCTTCTTTCGTGACGGCATGACCATCATGGTGGGCGGATTTATGGGGATTGGCACTCCCTCCCGCCTGGTTTAAACCTTACTGGAATCTGGTGTTCGCGACCTGACATTGATAGCCAATGATACCGCGTTTGTTGATACAGGCCTCGGTc                                                                                                                                                                         >  2:101061/1‑251 (MQ=255)                                                                                                                                      aaaaTTGATGACATTACAAGACGCCACCGGCTTCTTTCGTGACGGCATGACCATCATGGTGGGCGGATTTATGGGGATTGGCACTCCATCCCGCCTGGTTGAAACATTACTGGAATCTGGTGTTCGCGACCTGACATTGATAGCCAATGATACCGCGTTTGTTGATACCGGCATCGGTCCGCTCATCGTCAATGGTCGAGTCCGCAAAGTGATTGCTTCACATATCGGCACCAACCCGGAAACAGGTAGgc                                                                                                 >  1:222676/1‑251 (MQ=255)                                                                                                                                        aaTTGATGACATTACACGACGCCACCGGCTTCTTTCGTGACGGCATGCCCATCATGATGAGCGGATTTATGGGGATTGGCACTCCATACCGCCTGGTTGCAAAATTACTGGCATCTGGTGTTCGCGACCTGACATTGATAGCCACTGATACCGCGTTTGTTGATACCGGCATCGGTCCGATCATCGTCAATGGTCGAGTCCGCAAAGTGATTGCTTCACATATCGGCACCAACCAGGAAACAGGTCGgcgc                                                                                               >  2:95203/1‑251 (MQ=255)                                                                                                                                                 cATTACAAGACGCCACCGGCTTCTTTCGTGACGGCATGACCATCATGGTGGGCGGATTTATGGGGATTGGCACTCCATCCCGCCTGGTTGAAACATTACTGGAATCTGGTGTTCGCGACCTGACATTGATAGCCAATGATACCGCGTTTGTTGATACCGGCATCGGTCCGCTCATCGTCAATGGTCGAGTCCGCAAAGTGATTGCTTCACATATCGGCACCAACCCGGAAACAGGTCGGCGCATGATATCt                                                                                      <  2:207565/251‑1 (MQ=255)                                                                                                                                                                  ggCTTCTTTCGTGACGGCATGACCATCATGGTGGGCGGATTTATGGGGATTGGCACTCCATCCCGCCTGGTTGAAACATTACTGGAATCTGGTGTTCGCGACCTGACATTGATAGCCAATGATACCGCGTTTGTTGATACCGGCATCGGTCCGCTCATCGTCAATGGTCGAGTCCGCAAAGTGATTGCTTCACATATCGGCACCAACCCGGAAACAGGTCGGCGCATGATATCTGGTGAGATGGAcgtcgt                                                                     >  1:63447/1‑251 (MQ=255)                                                                                                                                                                                                                          tCCATCCCGCCTGGTTGAAACATTACTGGAATCTGGTGTTCGCGACCTGACATTGATAGCCAATGATACCGCGTTTGTTGCTACCGGCATCGGTACGCTCATCGTCAATGGTCGAGCCCGCAAAGTGATTGCTTCACATATCGGCACCAACCCGGACACAGGTCGGCGCATGATATCTGGTGAGCTGGACGTCGTTCTGGTGCCGCAAGGTACGCTAATCGAGCAAATTCGCTGTGGTGGAGCTGGACTtg             >  1:123138/1‑251 (MQ=255)                                                                                                                                                                                                                                 tgCCTGGTTGAAACATTACTGGAATCTGGTGTTCGCGACCTGACATTGATAGCCAATGATACCGCGTTTGTTGATACCGGCATCGGTCCGCTCATCGTCAATGGTCGAGTCCGCAAAGTGATTGCTTCACATATCGGCACCAACCCGGAAACAGGTCGGCGCATGATATCTGGTGAGATGGACGTCGTTCTGGGGCCGCAAGGTACGCTAATCGAGCAAATTCGCTGTGGTGGAGCTGGCCTTGGTGGttt      >  2:143593/2‑251 (MQ=255)                                                                                                                                                                                                                                   ccTGGTTGAAACATTACTGGAATCTGGTGTTCGCGACCTGACATTGATAGCCAATGATACCGCGTTTGTTGCTACCGGCATCGGTCCGCTCATCGTCAATGGTCGAGTCCGCAAAGTGATTGCTTCACATATCGGCACCAACCCGGAAACAGGTCGGCGCATGATATCTGGTGAGATGGACGTCGTTCTGGTGCCGCAAGGAACGCTAATCGAGCAAATTCGCTGTGGTGGAGCTGGACTTGGTGGttttt    >  2:112655/1‑250 (MQ=255)                                                                                                                                                                                                                                      ggTTGAAACCTTACTGGAATCTGGTGTTCGCGACCTGACATTGATAGCCAATGATACCGCGTTTGTTGATACCGGCATCGGTCCGCTCATCGTCACTGGTCGAGTCCGCAAAGTGATTGCTTCACATATCGGCACCAACCCGGAAACAGGTCGGCGCATGATCTCTGGTGAGATGGACGTAGTTCTGGTGCCGCAAGGTACGCTAATCGCGCAAATTCGCTGGGGTGGAGCTGGACTTGGTGGTTTtctc  >  2:189390/1‑250 (MQ=255)                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                   ATCCCCATCCAATTTATCCCTTCATATTCAATTAGTTAAATAACTAAATCCAATAATCTCATTCTGGCACTCCCCTTGCTATTGCCTGACTGTACCCACAACGGTGTATGCAAGAGGGATAAAAAATGAAAACAAAATTGATGACATTACAAGACGCCACCGGCTTCTTTCGTGACGGCATGACCATCATGGTGGGCGGATTTATGGGGATTGGCACTCCATCCCGCCTGGTTGAAGCATTACTGGAATCTGGTGTTCGCGACCTGACATTGATAGCCAATGATACCGCGTTTGTTGATACCGGCATCGGTCCGCTCATCGTCAATGGTCGAGTCCGCAAAGTGATTGCTTCACATATCGGCACCAACCCGGAAACAGGTCGGCGCATGATATCTGGTGAGATGGACGTCGTTCTGGTGCCGCAAGGTACGCTAATCGAGCAAATTCGCTGTGGTGGAGCTGGACTTGGTGGTTTTCTC  >  NC_000913/2323322‑2323800

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑