Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,222,148	G→A	intergenic (‑159/‑37)	bglX ← / → dld	beta‑D‑glucoside glucohydrolase, periplasmic/quinone‑dependent D‑lactate dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,222,148	0	G	A	100.0%	44.2 / NA	14	intergenic (‑159/‑37)	bglX/dld	beta‑D‑glucoside glucohydrolase, periplasmic/quinone‑dependent D‑lactate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (8/6);  total (8/6)

TAATGGATGGTTGCCGAATAAATCATCCGCCAGTGCTGGCTGCAGGGCCAGACTCACCGCGATTCCTACTGAACATAGCCATTTCATATGGATTTTTCTTCCTTTGTTGCCCGACGTGGCAGCGAAAATGGTGCAAAAACCGTAGTTTGCCATAAGCATGATGGAGAGAGAAAAAGAATGCTCAGTTTATTGTCTGAATTTTCAAAATATTCACTCGCTGAATTGTTATACAAGGCGCTATTCTAGTTTGTGATATTTTTTCGCCACCACAAGGAGTGGAAAATGTCTTCCATGACAACAACTGATAATAAAGCCTTTTTGAATGAACTTGCTCGTCTGGTGGGTTCTTCACACCTGCTCACCGATCCCGCAAAAACGGCCCGCTATCGCAAGGGCTTCCGTTCTGGTCAGGGCGACGCGCTGGCTGTCGTTTTCCCTGGCTCACTACTAGAATTGTGGCGGGTGCTGAAAGCCTGCGTCACCGC  >  NC_000913/2221903‑2222387                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                tAATGGATAGCTGCCGCATAAATCATCCGCCAGTGCTGGCTGCAGGGCCAGACTCACCGCGATTCCTACTGCACATAGCCATTTCATATGGATTTTTCTTCCTTTGTTGCCCGACGTGGCCGCGAAAATGGTGCAAAAACCGTAGTTTGCCATAAGCATGATGGAGAACGACAAAGAATGCTCAGTTTATTGTCTGAATTTTCACAATATTCACTCGCTGAATTGTTATACAAGGCGCTATTCTAATTtgt                                                                                                                                                                                                                                            >  2:101341/1‑251 (MQ=255)                                     tggctgCAGGGCCAGACTCACCGCGATTCCTACTGAACATAGCCATTTCATATGGATTTTTCTTCCTTTGTTGCCCGCCGTGGCAGCGAAAATGGTGCAAAACCCGTAGTTTGCCATCAGCATGATGGAGAGAGAAAACGAATGCTCAGTTTATTGTCTGAATTTTCAAAATATTCACTCGCTGAATTGTTATACAAGGCGCTATTCTAATTTGAGATATTTTTTAGCCACCACCAGGAGTGGAAAATGTc                                                                                                                                                                                                        >  2:41546/1‑251 (MQ=255)                                          gCAGGGCCAGACTCACCGCGATTCCTACTGAACATAGCCATTTCATATGGATTTTTCTTCCTTTGTTGCCCGACGTGGCAGCGAAAATGGTGCAAAAACCGTAGTTTGTCATAAGCATGATGGAGAGAGAAAAAGAATGCTCAGGTTATTGTCTGAATTTTCAAAATATTCACTCGCTGAATTGTTATACAAGGCGCTATTCTAATTTGTGATATTTTTTCGCCACCACAAGGAGTGGAAAATGTCTTcc                                                                                                                                                                                                    <  2:260276/250‑1 (MQ=255)                                                                   tACTGAACATAGCCATTTCATATGGATTTTTCTTCCTTTGTTGCCCGACGTGGCAGCGAAAATGGTGCAAAAACCGTAGTTTGCCATAAGCATGATGGAGAGCGAAAAAGAATGCTCAGTTTATTGTCTGAATTTTCAAAATATTCACTCGCTGAATTGTTATACAAGGCGCTATTCTAATTTGTGATATTTTTTCGCCACCACAAGGAGTGGAAAATGTCTTCCATGACAACAACTGATAATAAAGCCtt                                                                                                                                                                          >  1:110647/1‑251 (MQ=255)                                                                   tACTGAACATAGCCATTTCATATGGATTTTTCTTCCTTTGTTGCCCGACGTGGCAGCGAAAATGGTGCAAAAACCGTAGTTTGCCATAAGCATGATGGAGAGAGAAAAAGAATGCTCAGTTTATTGTCTGAATTTTCAAAATATTCACTCGCTGAATTGTTATACAAGGCGCTATTCTAATTTGTGATATTTTTTCGCCACCACAAGGAGTGGAAAATGTCTTCCATGACAACAACTGATAATAAAGCCtt                                                                                                                                                                          >  2:61927/1‑251 (MQ=255)                                                                       gAACATAGCCATTTCATATGGATTTTTCTTCCTTTGTTGCCCGACGTGGCAGCGAAAATGGTGCAAAAACCGTAGTTTGCCATAAGCATGATGGAGAGAGAAAAAGAATGCTCAGTTTATTGTCTGAATTTTCAAAATATTCACTCGCTGAATTGTTATACAAGGCGCTATTCTAATTTGTGATATTTTTTCGCCACCACAAGGAGTGGAAAATGTCTTCCATGACAACAACTGATAATAAAGCCttttt                                                                                                                                                                       <  1:47620/250‑1 (MQ=255)                                                                                                ttcttcCTTTGTTGCCCGACGTGGCAGCGAAAATGGTGCAAAAACCGTAGTTTGCCATAAGCATGATGGAGAGAGAAAAAGAATGCTCAGTTTATTGTCTGAATTTTCAAAATATTCACTCGCTGAATTGTTATACAAGGCGCTATTCTAATTTGTGATATTTTTTCGCCACCACAAGGAGTTGAAAATGTCTTCCATGACAACAACTGATAATAAAGCCTTTTTGAATGAACTTGCTCGTCTGGTGGGt                                                                                                                                              <  2:146634/250‑1 (MQ=255)                                                                                                              cccGACGTGGCAGCGAAAATGGAGCCAAAACCGTAGTTTGCCATACGCGTGATGGCGAGCGAAAAAGAATGCTCAGTTTCTTGTCTGAATTTTCAAAATCTTCAATCGCTGAATTGTTATCCAAGGCGCTATTCTCATTTGTGATATTTTTTCGCCACCACAAGGAGTGGAAAATGTCTTCCATGACAACAACTGATAATAAAGCCTTTTTGAATGAACTTGCTCGTCTGGTGGGTTCTTCACACCTGCTc                                                                                                                               >  2:13121/1‑251 (MQ=255)                                                                                                                                   gTGCAAAAACCGTAGTTTGCCATAAGCATGATGGAGAGAGAAAAATAATGCTCAGTTTATTGTCTGAATTTTCAAAATATTCACTCGCTGAATTGTTATACAAGGCGCTATTCTAATTTGTGATATTTTTTCGCCACCACAAGGAGTGGAAAATGTCTTCCATGACAACAACTGATAATAAAGCCTTTTTGAATGAACTTGCTCGTCTGGTGGGCTCTTCACACCTGCTCACCTATCCCGCAAAAACGGcc                                                                                                          <  2:12862/251‑1 (MQ=255)                                                                                                                                                                                                    gAATTTTCAAAATATTCACTCGCTGAATTGTTATACAAGGCGCTATTCTAATTTGTGATATTTTTTCGCCACCACAAGGAGTGGAAAATGTCTTCCATGACAACAACTGATAATAAAGCCTTTTTGAATGAACTTGCTCGTCTGGTGGGTTCTTCACACCTGCTCACCGATCCCGCAAAAACGGCCCGCTATCGCAAGGGCTTCCGTTCTGGTCAGGGCGACGCGCTGGCTGTCGTTTTCCCTGGCTCact                                         >  1:122578/1‑251 (MQ=255)                                                                                                                                                                                                                    cACTCGCTGAATTGTTATACAAGGCGCTATTCTAATTTGTGATATTTTTTCGCCACCACAAGGAGTGGAAAATGTCTTCCATGACAACAACTGATAATAAAGCCTTTTTGAATGAACTTGCTCGTCTGGTGGGTTCTTCACACCTGCTCACCGATCCCGCAAAAACGGCCCGCTATCGCAAGGGCTTCCGTTCTGGTCAGGGCGACGCGCTGGCTGTCGTTGTCCCTGGCTCACTACTAGAATTGTGGCgg                         <  1:208887/251‑1 (MQ=255)                                                                                                                                                                                                                          cTGAATTGTTCTAAAAGGAGCTCTTCTAATTTGTGATATTTTTTCGACACAACAAGGAGTGCAAAATGTCTTCCATGACAACAACTGATACTAACGCCTTTTTGAATGAACTTGCTCGTCTGGTGGGTTCTTCCCACCTGCTCACCGATCCCGCACAAACGGCCCACTATCGCAAGGGCTTCCGTTCTGGTCAGGGCGACGCGCTGGCTGTGGTTTTCCCTGGCTCACTACTAGACTTGCGGCggggggtg                   >  2:238319/1‑246 (MQ=255)                                                                                                                                                                                                                                          ggCGCTATTCTAATGTGTGATATTTTGTCGCCAACACAAGGAGTGGAAAATGTCTTCCATGACAACAACTGATAATAAAGCCTTTTTGAATGAACTGGCTCGGCTGGTGGGTTCTTCACACCTGCTCACCGATCCCGCAAAAACGGCCCGCTATCGCAAGGGCTTCCGTTCTGGTCAGGGCGACGCGCTGGCTGTCGTTTTCCCTGGCTCTCTACTAGAATTGTGGCGGGTACTGAAAGCCTGCGTCAccg   <  1:95037/251‑1 (MQ=255)                                                                                                                                                                                                                                           gcgcTATTCTAATTTGTGATATTTTTTCGCCACCACAAGGAGTGGAAAATGTCTTCCATGACAACAACTGATAATAAAGCCTTTTTGAATGAACTTGCTCGTCTGGTGGGTTCTTCACATCTGCTCACCGATCCCGCAAAAACGGCCCGCTATAGCAAGGGCTTCCGTTCTGGTCAGGGCGACGCGCTGGCTGTCGTTTTCCCTGGCTCACTACTAGAATTGTGGCggcggcttgaagaacgcgccacccc  >  1:90341/1‑228 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                TAATGGATGGTTGCCGAATAAATCATCCGCCAGTGCTGGCTGCAGGGCCAGACTCACCGCGATTCCTACTGAACATAGCCATTTCATATGGATTTTTCTTCCTTTGTTGCCCGACGTGGCAGCGAAAATGGTGCAAAAACCGTAGTTTGCCATAAGCATGATGGAGAGAGAAAAAGAATGCTCAGTTTATTGTCTGAATTTTCAAAATATTCACTCGCTGAATTGTTATACAAGGCGCTATTCTAGTTTGTGATATTTTTTCGCCACCACAAGGAGTGGAAAATGTCTTCCATGACAACAACTGATAATAAAGCCTTTTTGAATGAACTTGCTCGTCTGGTGGGTTCTTCACACCTGCTCACCGATCCCGCAAAAACGGCCCGCTATCGCAAGGGCTTCCGTTCTGGTCAGGGCGACGCGCTGGCTGTCGTTTTCCCTGGCTCACTACTAGAATTGTGGCGGGTGCTGAAAGCCTGCGTCACCGC  >  NC_000913/2221903‑2222387

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑