Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,051,845	G→A	W2303* (TGG→TAG)	yeeJ →	inverse autotransporter adhesin

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,051,845	0	G	A	100.0%	26.1 / NA	10	W2303* (TGG→TAG)	yeeJ	inverse autotransporter adhesin
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (4/6);  total (4/6)

AAATGTCGGCAGCAATTCGGAAAGGATTACGGCGACGCCAAAATCAGGAGGCCCTAGCTATGTATACGAAATCCGTGTGAAGAGTTGGTGGGTGAACGCCGGCGAGGCTTTCATGATATACAGCCTTGCTGAAAATTTTTGCAGCAGCAATGGCTACACGCTCCCCAGAGCAAACTATTTAAACCACTGTAGTTCCCGAGGCATCGGGTCACTGTACAGTGAATGGGGAGATATGGGGCATTACACGACTGACGCTGGTTTTCAATCAAATATGTATTGGTCATCTAGTCCCGCAAACTCAAGCGAACAATACGTAGTTTCCCTGGCAACAGGTGATCAAAGCGTATTTGAAAAGCTTGGGTTTGCTTATGCGACATGTTATAAAAACCTGTGATTTTCCGTTATAATTTCTTAAAGAGTGTTCCATTCGGGGCACTCTTTTTTACATTCCCTCT  >  NC_000913/2051621‑2052075                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                       aaaTGGCGGCAGCAATGCGGAAAGGATTACGGCGACGCCAAAATCAGGAGGCCCTAGCTATGTATACGAAATCCGTGTGAAGAGTTGGTGGGTGAACGCCGGCGAGGCTTTCATGATATACAGCCTTGCTGAAAATTTTTGCAGCAGCAATGGCTACACGCTCCCCAGAGCAAACTATGTAAACCACTGTAGGTCCCGAGGCATCGGGTCACTGTACAGTGAATAGGGAGATTTGGTGCAGTACACGACTg                                                                                                                                                                                                              <  2:116897/251‑1 (MQ=255)         gcagcaATTCGGAAAGGATTACGGCGACGCCAAAATCAGGAGGCCCTAGCTATGTATACGAAATCCGTGTGAAGAGTTGGTGGGTGAACGCCGGCGAGGCTTTCATGATATACAGCCTTGCTGAAAATTTTTGCAGCAGCAATGGCTACACGCTCCCCAGAGCAAACTATTTAAACCACTGTAGTTCCCGAGGCATCGGGTCACTGTACAGTGAATAGGGAGATATGGGGCATTACACGACTGACGCTGGt                                                                                                                                                                                                      <  1:169861/251‑1 (MQ=255)                                            tCAGGAGGCCCTAGCTATGTATACGAAATCCGTGTGAAGAGTTGGTGGGGGAACGCCGGCGAGGCATGCATGCTATACAGCCTTACTGAGAATTTTTGCAGCAGCAATGGCTACAGGCTCTCAGGTGCAACCTATTTAAACCATTGTAGTTCCCGAAGCATCGGGTCTCTGTACAGTGAGTAGGGAGATATGGAGCATGACACGACTTACGCTGGTTTTCAAAAAAATATGTATTGGTCATCAAGTcc                                                                                                                                                                      >  1:254481/1‑248 (MQ=255)                                                                            tgtgAAGAGTTGGTGGGTGAACGCCGGCGAGGCTTTCATGATATACAGCCTTGCTGAAAATTTTTGCAGCAGCAATGGCTACACGCTCCCCAGAGCAAACTATTTAAACCACTGTAGTTCCCGAGGCATCGGGTCACTGTACAGTGAATAGGGAGATATGGGGCATTACACGACTGACGCTGGTTTTCAATCAAATATGTATTGGTCATCTAGTCCCGCAAACTCAAGCGAACAATACGTAGTTTCCCTgg                                                                                                                                   >  2:192638/1‑251 (MQ=255)                                                                                    gTTGGTGGGTGAACGCCGGCGAGGCTTTCATGATATACAGCCTTGCTGAAAATTTTTGCAGCAGCAATGGCTACACGCTCCCCAGAGCAAACTATTTAAACCACTGTAGTTCCCGAGGCATCGGGTCACTGTACAGTGAATAGGGAGATATGGGGCATTACACGACTGACGCTGGTTTTCAATCAAATATGTATTGGTCATCTAGTCCCGCAAACTCAAGCGAACAATACGTAGTTTCCCTGGCAACAGGt                                                                                                                           <  2:198547/251‑1 (MQ=255)                                                                                      tggaggttgAACGCCGGCGAGGCTTTCATGATATACAGCCTTGCTGAAAATTTTTGCAGCAGCAATGGCTACACGCTCCCCAAAGCAACATATTTAAACCACTGTAGTTCCCGAGGAATCGGGTCACTGTACAGTGAATAGGGAGGTATGGGGCATTAATCGACTGACGCTGGTTTTAAATCAAATATGTATTGGTCATATCGACACGCAAACTAAAGCGAACAATACGCAGTTTCACTGGAACCAGGta                                                                                                                          >  2:8658/8‑249 (MQ=255)                                                                                                                                      aaTTTTTGCAGCAGCAATGGCTACACGCTCCCCAGAGCAAACTATTTAAACCACTGTAGTTCCCGAGGCATCGGGTCACTGTACAGTGAATAGGGAGATATGGGGCATTACACGACTGACGCTGGTTTTCAATCAAATATGTATTGGTCATCTAGTCCCGCAAACTCAAGCGAACAATACGTAGTTTCCCTGGCAACAGGTGAt                                                                                                                        >  1:236700/1‑204 (MQ=255)                                                                                                                                      aaTTTTTGCAGCAGCAATGGCTACACGCTCCCCAGAGCAAACTATTTAAACCACTGTAGTTCCCGAGGCATCGGGTCACTGTACAGTGAATAGGGAGATATGGGGCATTACACGACTGACGCTGGTTTTCAATCAAATATGTATTGGTCATCTAGTCCCGCAAACTCAAGCGAACAATACGTAGTTTCCCTGGCAACAGGTGAt                                                                                                                        <  2:236700/204‑1 (MQ=255)                                                                                                                                                                             aactagtgaatccACTGTAGTTCCCGAGGCATCGTGTCACTGTACAGTGAATAGGGAGATATGGGGCATTACACGACTGACGCTGGTTTTCAATCAAAAATGTATTGGTCATCTAGTCCCGCAAACTCAAGCGAACAATACGTAGTTTCCCTGGCAACAGGTGATCAAAGCGTATTTGAAAAGCTTGGGTTTGCTTATGCGACATGGGATAAAAACCTTTGATTTTCCGTTATAATTTCTTAAAGAGTGtt                                  <  1:223350/240‑1 (MQ=255)                                                                                                                                                                                                             cGGGTCACTGTACAGTGAATAGGGAGATATGGGGCATTACACGACTGACGCTGGTTTTCAAGCAAATATGTATTGGTCATCTAGTCCCGCAAACTCAAGCGAACAATACGTAGTTTCCCTGGCAACAGGTGATCAAAGCGTATTTGAAAAGCTTGGGTTTGCTTATGCGACATGTTATAAAAACCTGTGATTTTCCGTTATAATTTCTTAAAGAGTGTTCCATTCGGGGCACTCTTTTTTACATTCCctct  <  1:144850/251‑1 (MQ=255)                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                       AAATGTCGGCAGCAATTCGGAAAGGATTACGGCGACGCCAAAATCAGGAGGCCCTAGCTATGTATACGAAATCCGTGTGAAGAGTTGGTGGGTGAACGCCGGCGAGGCTTTCATGATATACAGCCTTGCTGAAAATTTTTGCAGCAGCAATGGCTACACGCTCCCCAGAGCAAACTATTTAAACCACTGTAGTTCCCGAGGCATCGGGTCACTGTACAGTGAATGGGGAGATATGGGGCATTACACGACTGACGCTGGTTTTCAATCAAATATGTATTGGTCATCTAGTCCCGCAAACTCAAGCGAACAATACGTAGTTTCCCTGGCAACAGGTGATCAAAGCGTATTTGAAAAGCTTGGGTTTGCTTATGCGACATGTTATAAAAACCTGTGATTTTCCGTTATAATTTCTTAAAGAGTGTTCCATTCGGGGCACTCTTTTTTACATTCCCTCT  >  NC_000913/2051621‑2052075

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑