Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,000,331	G→A	L19L (CTG→TTG)	tcyJ ←	cystine ABC transporter periplasmic binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,000,331	0	G	A	100.0%	33.7 / NA	12	L19L (CTG→TTG)	tcyJ	cystine ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (5/7);  total (5/7)

CAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATGTTTTGCCAGCTGTTGGGCAAATTCCACTTCAAAACCGGTTAATTTGCCGTCATCTCCCTGAAAACTGAACGGCGGATAAGTTCCTTCCAGCCCTACCAGCAGCGTGCCGCGCTCTTTAACTTTATTAAGCAGACCTTCATCTGCAAAACTTTTAACGCTCATGCCCGCAACCAGCGCCACGGCCATCACACCCATCAATGCCTGACGTCCCAGATGTGCTAATTTCATATTCACCCCGAATGTTGTTATGTCTGTTTGCAGTGTAGAGCCATTGTTTGTAAACACAAAAACAACTCCGCTACATCTTATTCTTATTTAATATATATCAGAAGAAGGCAGGCTGGAGGATTTCTGCACAAGTCTGGTACAGGTTTGGCGCTGATAGTGTTGATACTTCCGTAACGCG  >  NC_000913/2000098‑2000563                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                         cAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATGTTTTGCCAGCTGTTGGGCAAATTCCACTTCAAAACCGGTTAATTTGCCGTCATCTCCCTGAAAACTGAACGGCGGATAAGTTCCTTCCAGCCCTACCAGCAGCGTGCCGCGCTCTTTAACTTTATTAAGCAGACCTTCATCTGCAAAACTTTTAACGCTCATGCCCGCAACCAACGCCACGGCCCTCacac                                                                                                                                                                                                                         >  2:155700/1‑251 (MQ=255)         ccAGCATACCGGCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATGTTTTGCCAGCTGTTGGGCAAATTCCACTTCAAAACCGGTTAATTTGCCGTCATCTCCCTGAAAACTGAACGGCGGATAAGTTCCTTCCAGCCCTACCAGCAGCGTGCCGCGCTCTTTAACTTTATTAAGCAGACCTTCATCTGCAAAACTTTTAACGCTCATGCCCGCAACCAACGCCCCGGCCATCACACCCATCAAt                                                                                                                                                                                                                 <  2:177304/251‑1 (MQ=255)          cAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATGTTTTGCCAGCTGTTGGGCAAATTCCACTTCAAAACCGGTTAATTTGCCGTCATCTCCCTGAAAACTGAACGGCGGATAAGTTCCTTCCAGCCCTACCAGCAGCGTGCCGCGCTCTTTAACTTTATTAAGCAGACCTTCATCTGCAAAACTTTTAACGCTCATGCCCGCAACCAACGCCACGGCCATCACACCCATCAATg                                                                                                                                                                                                                >  1:36348/1‑251 (MQ=255)             cATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATGTTTTGCCAGCTGTTGGGCAAATTCCACTTCAAAACCGGTTAATTTGCCGTCATCTCCCTGAAAACTGAACGGCGGATAAGTTCCTTCCAGCCCTACCAGCAGCGTGCCGCGCTCTTTAACTTTATTAAGCAGACCTTCATCTGCAAAACTTTTAACGCTCATGCCCGCAACCAACGCCACGTCCATCACACCCATCAATGCCt                                                                                                                                                                                                             >  2:202355/1‑251 (MQ=255)                                                                        gTTGGGCAAATTCCACTTCAAAACCGGTTAATTTGCCGTCATCTCCCTGAAAACTGAACGGCGGATAAGTTCCTTCCAGCCCTACCAGCAGCGTGCCGCGCTCTTTAACTTTATTAAGCAGACCTTCATCTGCAAAACTTTTAACGCTCATGCCCGCAACCAACGCCACGGCCATCACACCCATCAATGCCGGACGTCCCAGATTTGCTAATTTCATATTCACCCCGAATGTTGTTATGTCTGTTTGCAgt                                                                                                                                                  <  1:38714/251‑1 (MQ=255)                                                                                           aaaaCCTGGTAATTTGCCGTCATCTCCCTGAAAACTGAACGGCGTATAAGTTCCTTCCAGCCCTACCAGCAGCGTGCCGCGCTCTTTAACTTTATTAAGCAGCCCTTCATCTGCAAAACTTTTAACGCTCATGCCCTCAACCAACGCCACGGCCATCACACCCATCAATGCCTGACGTCCCAGATGTGCTAATTTCATATTCACCCCGAATGTTGTTATGTCTGTTTGCAGTGTAGAGCCATTGTTTGTaa                                                                                                                               <  2:141283/251‑1 (MQ=255)                                                                                            aaacctggtAATTTGCCGTCATCTCCCTGAAAACTGAACGGAGGATAAGTTCCTTCCAGCCCTACCAGCAGCGTGCCGCGCTCTTTAACTTTATTAAGCCGACCTTCATCTGCAAAACTTTTAACGCTCATGCCCGCACCCAACGCCACGTCCATCACTCCCATCAATTCTTGACGTCCCAGATTTGCTAATTTCAGATTCACCCCGAATTTTGTTATGTCTGTTTGCAGTGTAGAGCCATTGTTTGTata                                                                                                                              <  2:9860/243‑3 (MQ=255)                                                                                                                                                  tccAGCCCTACCAGCAGCGTGCCGCGCTCTTTAACTTTATTAAGCAGACCTTCATCTGCAAAACTTTTAACGCTCATGCCCGCAACCAACGCCACGGCCATCACACCCATCAATGCCTGACGTCCCAGATGTGCTAATTTCATATTCACCCCGAATGTTGTTATGTCTGTTTGCAGTGTAGAGCCATTGTTTGTAAACACAAAAACAACTCCGCTACATCTTATTCTTATTTAAGATATATCAgaagaa                                                                          <  2:117332/249‑1 (MQ=255)                                                                                                                                                                                           aaGCAGACCTTCATCTGCAAAACTTTTAACGCTCATGCCCGCAACCAACGCCACGGCCATCACACCCATCAATGCCTGACGTCCCAGATGTGCTAATTTCATATTCACCCCGAATGTTGTTATGTCTGTTTGCAGTGTAGAGCCATTGTTTGTAAACACAAAAACAACTCCGCTACATCTTATTCTTATTTAatatatat                                                                                  <  1:78162/200‑1 (MQ=255)                                                                                                                                                                                           aaGCAGACCTTCATCTGCAAAACTTTTAACGCTCATGCCCGCAACCAACGCCACGGCCATCACACCCATCAATGCCTGACGTCCCAGATGTGCTAATTTCATATTCACCCCGAATGTTGTTATGTCTGTTTGCAGTGTAGAGCCATTGTTTGTAAACACAAAAACAACTCCGCTACATCTTATTCTTATTTAatatatat                                                                                  >  2:78162/1‑200 (MQ=255)                                                                                                                                                                                                    ttCATCTGCAAAACTTTTAACGCTCATGCCCGCAACCAACGCCACGGCCATCACACCCATCAATGCCTGACGTCCCAGATGTGCTAATTTCATATTCACCCCGAATGTTGTTATGTCTGTTTGCAGTGTAGAGCCATTGTTTGTAAACACAAAAACAACTCCGCTACATCTTATTCTTATTTAATATATATCAGAAGAAGGCAGGCTGGAGGATTTCTGCACAAGTCTGGTACAGGTTTGGCGCTGAt                         >  1:175440/1‑248 (MQ=255)                                                                                                                                                                                                                        cGCTCATGCCCGCAACCAACGCCACGGCCATCACACCCATCAATGCCTGACGTCCCAGATGTGCTAATTTCATATTCACCCCGAATGTTGTTATGTCTGTTTGCAGTGTAGAGCCATTGTTTGTAAACACAAAAACAACTCCGCTACATCTTATTCTTATTTAATATATATCAGAAGAAGGCAGGCTGGAGGATTTCTGCACAAGTCTGGTACAGGTTTGGCGCTGATAGTGTTGATACTTCCGTAAcgcg  <  1:2449/251‑1 (MQ=255)                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                         CAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATGTTTTGCCAGCTGTTGGGCAAATTCCACTTCAAAACCGGTTAATTTGCCGTCATCTCCCTGAAAACTGAACGGCGGATAAGTTCCTTCCAGCCCTACCAGCAGCGTGCCGCGCTCTTTAACTTTATTAAGCAGACCTTCATCTGCAAAACTTTTAACGCTCATGCCCGCAACCAGCGCCACGGCCATCACACCCATCAATGCCTGACGTCCCAGATGTGCTAATTTCATATTCACCCCGAATGTTGTTATGTCTGTTTGCAGTGTAGAGCCATTGTTTGTAAACACAAAAACAACTCCGCTACATCTTATTCTTATTTAATATATATCAGAAGAAGGCAGGCTGGAGGATTTCTGCACAAGTCTGGTACAGGTTTGGCGCTGATAGTGTTGATACTTCCGTAACGCG  >  NC_000913/2000098‑2000563

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑