Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,643,679	A→T	L209Q (CTG→CAG)	ydfU ←	protein YdfU

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,643,679	0	A	T	95.7%	71.9 / ‑6.5	23	L209Q (CTG→CAG)	ydfU	protein YdfU
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base T (12/10);  minor base C (1/0);  total (13/10)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GATCGTCGGCTGGCATACCGCAGCAAGCACACGGCTGTGTCTTAACCCAACGTGTGTATTTCTCCTTAACCCAGCGGCGACGTTTAGGCAGCTTCATGAAAGATTCCGGAGACTCTGGATCAACGGTGATGCTTACCACCGTCTTTTCCTGTGGTGATTTTTGTTGCTGGTGGGCGTAAGGCAACGGTGCAAGATTTTTTGTGCGTTGTTTCAATATGCTGGTGGCGGTCAGCTCTCCCGGTACGATGTCGCTTTCGCGGTACACCGAGCA‑GATTTTTTCCGCTGGTAATCCCAGCGAACGACGCGATACAGCCTCAGGTAGTGCATCCACCACCTGATTGCAGACCGCCCACCAGGATAATTCAGCCAAAGATAATTCCCGCTCCTGCGTACCGCTTATTGCGTGACGGA‑TGACGTCAATCACCCATGCTGTCAGATTTTGTTGAGCAAGCAGCTCCAGTGATTCCGATGTCTGGT  >  NC_000913/1643449‑1643925                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                         gATCGTCGGCTGGCATTCCGCAGCAAGCACACGGCTGTGTCTTAACCCAACGTGTGTATTTCTCCTTAACCCAGCGGCGACGTTTAGGCAGCTTCATGAAAGATTCCGGAGACTCTGGATCAACGGTGATGCTTACCACCGTCTTTTCCTGTGGTGATTTTTGTTGCTGGTGGGCGTAAGGCAACGGTGCAAGATTTTTTGTGCGTTGTTTCAATATGCTGGTGGCGGTCTGCTCTCCCGGTACGATGTCg                                                                                                                                                                                                                                      <  1:21350/251‑1 (MQ=255)           tGGCATACCGCAGCAAGCACACGGCTGTGTCTTAACCCAACGTGTGTATTTCTCCTTAACCCAGCGGCGACGTTTAGGCAGCTTCATGAAAGATTCCGGAGACTCTGGATCAACGGTGATGCTTACCACCGTCTTTTCCTGTGGTGATTTTTGTTGCTGGTGGGCGTAAGGCAACGGTGCAAGATTTTTTGTGCGTTGTTTCAATATGCTGGTGGCGGTCTGCTCTCCCGGTACGATGTCGCTTTCGCGGt                                                                                                                                                                                                                            >  1:69953/1‑251 (MQ=255)                        caagcaCACGGCTGTGTCTTAACCCAACGTGTGTATTTCTCCTTAACCCAGCGGCGACGTTTAGGCAGCTTCATGAAAGATTCCGGAGACTCTGGATCAACGGTGATGCTTACCACCGTCTTTTCCTGTGGTGATTTTTGTTGCTGGTGGGCGTAAGGCAACGGTGCAAGATTTTTTGTGCGTTGTTTCAATATGCTGGTGGCGGTCTGCTCTCCCGGTACGATGTCGCTTTCGCGGTACACCGAGc                                                                                                                                                                                                                   >  1:148913/1‑247 (MQ=255)                                  gCTGTGTCTTAACCCAACGTGTGTATTTCTCCTTAACCCAGCGGCGACGTTTAGGCAGCTTCCTGAATGATTCCGGAGACTCTGGATCACCGGTGCTGCTTCCCACCGTCTTTTCCTGTGGTGATTTTTGTTGCTGGTGGGCGTAAGGCAACGGTGCAAGATTTTTTGTGCGTTGTTTCAATATGCTGGTGGCGGTCTGCTCTCCCGGCACGATGTCGCTTTCGCGGTACACCGAGCA‑GATTTTTTc                                                                                                                                                                                                        >  1:106408/1‑247 (MQ=255)                                  gCTGTGACTTAACCCAACGTGTGTATTTCTCCGTAACCCAGCGGCGACGTTTAGGCAGCTTCATGAAAGATGCCGGAGACTCTGGATCAACGGTGATGCTTACCACCGTCTTTTCCTGTGGTGATTTGTGTTGCTGGTGGGCGTAAGGCAACGGTGCAAGATTTTTTGTGCGTTGTTTCAATATGCTGGTGGCGGTGTGCTCTCCCGGGACGATGTCGCGGTCGCGGTACACCGAGCA‑GATTTTTTc                                                                                                                                                                                                        <  2:106408/247‑1 (MQ=255)                                                                                             ttCATGAAAGATTCCGGAGACTCTGGATCAACGGCGATGCTTACCACCGTCTTTTCCTGTGGTGATTTTTGTTGCTGGTGGGCGTAAGGCAACGGTGCAAGATTTTTTGTGCGTTGTTTCAATATGCTGGTGGCGGTCTGCTCTCCCGGTACGATGTCGCTTTCGCGGTCCACCGAGCA‑GATTTTTTCCGCTGGTAATCCCAGCGAACGCCGCGATACAGCCTCAGGTAGTGCATCCACCACCTGATTGc                                                                                                                                          >  2:160424/1‑250 (MQ=255)                                                                                                         tCCGGAGAATCTGGATCAACGGTGATGCTTACCACCGTCTTTTCCTGTGGTGATTTTTGTTGCTGGTGGGCGTAAGGCAACGGTGCAAGATTTTTTGTGCGTTGTTTCAATATGCTGGTGGCGGTCTGCTCTCCCGGTACGATGTCGCTTTCGCGGTACACCGAGCA‑GATTTTTTCCGCTGGTAATCCCAGCGAACGACGCGATACAGCCTCAGGTAGTGCATCCACCACCTGATTGCAGACCGCccacc                                                                                                                              >  2:3613/1‑250 (MQ=255)                                                                                                                             ggTGATGCTTACCACCGTCTTTTCCTGTGGTGATTTTTGTTGCTGGTGGGCGTAAGGCAACGGTGCAAGATTTTTTGTGCGTTGTTTCAATATGCTGGTGGCGGTCTGCTCTCCCGGTACGATGTCGCTTTCGCGGTACACCTAGCA‑GATTTTTTCCGCTGGTAATCCCAGCGAACGACGCGATACAGCCTCAGGTAGTGCATCCACCACCTGATTGCAGACCGCCCACCAGGATAATTCAGCCAAAGATa                                                                                                         <  1:67578/251‑1 (MQ=255)                                                                                                                              gTGATGCTTACCACCGTCTTTTCCTGTGGTGATTTTTGTTGCTGGTGGGCGTAAGGCAACGGTGCAAGATTTTTTGTGCGTTGTTTCAATATGCTGGTGGCGGTCTGCTCTCCCGGTACGATGTCGCTTTCGCGGTACACCGAGCA‑GATTTTTTCCGCTGGTAATCCCAGCGAACGACGCGATACAGCCTCAGGTAGTGCATCCACCACCTGATTGCAGACCGCCCACCAGGATAATTCAGCCAAAGATa                                                                                                         >  1:8719/1‑250 (MQ=255)                                                                                                                                  ggCTTACCACCGTCTTTTCCTGTGGTGATTTTTGTTGCTGGTGGGCGTAAGGCAACGGTGCAAGATTTTTTGTGCGGTGTTTCAATATGCTGGTGGCGGTCTGCTCTCCCGGTACGATGTCGCTTTCGCGGTACACCGAGCA‑GATTTTTTCCGCTGGTAATCCCAGCGAACGACGCGATACAGCCTCAGGTAGTGCATCCACCACCTGATTGCAGACCGCCCACCAGGATAATTCAGCCAAAGATAATTcc                                                                                                    <  2:161895/250‑1 (MQ=255)                                                                                                                                         caccGTCTTTTCCTGTGGTGATTTTTGTTGCTGGTGGGCGTAAGGCAACGGTGCAAGATTTTTTGTGCGTTGTTTCAATATGCTGGTGGCGGTCTGCTCTCCCGGTACGATGTCGCTTTCGCGGTACACCGAGCA‑GATTTTTTCCGCTGGTAATCCCAGCGAACGACGCGATACAGCCTCAGGTAGTGCATCCACCACCTGATTGCAGACCGCCCACCAGGATAATTCAGCCAAAGATAATTCCCGCTCCt                                                                                             >  1:72610/1‑251 (MQ=255)                                                                                                                                                      tgtgGTGATTTTTGTTGCTGGTGGGCGTAAGCCAACGGTGCAAGATTTTTTGTGCGTTGTTTCAATATGCTGGTGGCGGTCTGCTCTCCCGGTACGATGTCGCTTTCGCGGTACACCGAGCA‑GATTTTTTCCGCTGGTAATCCCAGCGAACGACGCGATACAGCCTCAGGTAGTGCATCCACCACCTGATTGCAGACCGCCCACCAGGATAATTCAGCCAAAGATAATTCCCGCTCCTGCGTACCGCTTAt                                                                                >  1:217016/1‑251 (MQ=255)                                                                                                                                                         ggtgATTTTTGTTGCTGGTGGGCGTAAGGCAACGGTGCAAGATTTTTTGTGCGTTGTTTCAATATGCTGGTGGCGGTCTGCTCTCCCGGTACGATGTCGCTTTCGCGGTACACCGAGCA‑GATTTTTTCCGCTGGTAATCCCAGCGAACGACGCGATACAGCCTCAGGTAGTGCATCCACCACCTGATTGCAGACCGCCCACCAGGATAATTCAGCCAAAGATAATTCCCGCt                                                                                                >  1:256053/1‑232 (MQ=255)                                                                                                                                                         ggggATTTTTGTTGCTGGTGGGCGTAAGGCAACGGTGCAAGATTTTTTGTGCGTTGTTTCAATATGCTGGGGGCGGTCTGCTCTCCCGGTACGATGTCGCTTTCGCGGTACACCGAGCA‑GATTTGTTCCGCTGGTAATCCCAGCGAACGACGCGATACAGCCTCAGGTAGTGCATCCACCACGTGATTGCAGACCGCCCACCAGGATAATTCAGCCAAAGATAATTCCCGCt                                                                                                <  2:256053/229‑1 (MQ=255)                                                                                                                                                             aTTTTTGTTGCTGGTGGGCGTAAGGCAACGGTGCAAGATTTTTTGTGCGTTGTTTCAATATGCTGGTGGCGGTCTGCTCTCCCGGTACGATGTCGCTTTCGCGGTACACCGAGCA‑GATTTTTTCCGCTGGTAATCCCAGCGAACGACGCGATACAGCCTCAGGTAGTGCATCCACCACCTGATTGCAGACCGCCCACCAGGATAATTCAGCCAAAGATAATTCCCGCTCCTGCGTTCCGCTTATTGCGTGa                                                                         <  1:169571/251‑1 (MQ=255)                                                                                                                                                                         ggtggGCGTAAGGCAACGGTGCAAGATTTTTTGTGCGTTGTTTCAATATGCTGGTGGCGGTCTGCTCTCCCGGTACGATGTCGCTTTCGCGGTACACCGAGCA‑GATTTTTTCCGCTGGTAATCCCAGCGAACGACGCGATACAGCCTCAGGTAGTGCATCCACCACCTGATTGCAGACCGCCCACCAGGATAATTCAGCCAAAGATAATTCCCGCTCCTGCGTACCGCTTATTGCGTGACGGA‑TGACGTc                                                             <  2:69953/250‑1 (MQ=255)                                                                                                                                                                            ggtagtcaGGCAACGGTGCAAGATTTATTGTGCGTTGTTTCAATATGCTGGTGGCGGTCTGCTCTCCCGGTACGATTGCGCTTTCGCGGTACAAAGAGAA‑GATTTTTTCCGCTGGTAATCCCAGCGAACGCCGGGATACAGCCTTAGGTAGTGCATCCACAACCTGATTGCAGACCGCCCACCAGGTTAATTCAGCCAAAGATAATTCCCGCTCCTGAGAACCGCTTATTGCGTG‑CGGACTGACGTCAATc                                                         >  2:176576/8‑251 (MQ=255)                                                                                                                                                                               cGTAAGGCAACGGTGCAAGATTTTTTGTGCGTTGTTTCAATATGCTGGTGGCGGTCTGCTCTCCCGGTACGATGTCGCTTTCGCGGTACACCGAGCA‑GATTTTTTCCGCTGGTAATCCCAGCGAACGACGCGATACAGCCTCAGGTAGTGCATCCACCACCTGATTGCAGACCGCCCACCAGGATAATTCAGCCAAAGATAATTCCCGCTCCTGCGTACCGCTTATTGCGTGACGGA‑TGACGTCAATCAcc                                                      <  2:74679/251‑1 (MQ=255)                                                                                                                                                                                     gCAACGGTGCGAGATTTTTTGTGCGTTGTTTCAATATGCTGGTGGCGGTCTGCTCTCCCGGTACGATGTCGCTTTCGCGGTACACCGAGCA‑GATTTTTTCCGCTGGTAATCCCAGCGAACGACGCGATACAGCCTCAGGTAGTGCATCCACCACCTGATTGCAGACCGCCCACCAGGATAATTCAGCCAAAGATAATTCCCGCTCCTGCGTACCGCTTATTGCGTGACGGA‑TGACGTCAATCACCCATGCt                                                <  2:198359/251‑1 (MQ=255)                                                                                                                                                                                             gcaagctgttatgaccgtagttTCAATATGCTCGTGCCGGTCTGCTCTACCGCGACGATGTCCCTTTCCCGGCACCACGA‑CACGATTTTTTCCGCTGGTAATCCCAGCGACCGACGCGATCCAGCCTCAGGTAGTGCATCCACCACCTGATTGCAGCCCGACCACCAGGCTAATTCAGCCAAAGATAATTACCGCTCCTGCGTACCGCTTCTTGCGTGACGGA‑TGACGTCAATCACACATCCTGTCAGAtt                                        >  2:251650/20‑251 (MQ=255)                                                                                                                                                                                                      tttGTGCGTTGTTTCAATATGCTGGTGGCGGTCCGCTCTTCCGGTGCGATGTAGCTTTCGCGGTACACCGAGCA‑GATTTTTTCCGCTGGTAATCCCAGCGAACGACGCGCTACAGCCTCAGGTAGTGCATCCACAACCTGATTGCAGACCGCCCACCAGGATAATTCAACCAAAGATAATTCCCGCTCCTGCGTACCGCTTATGGCGTGACGGA‑TGACGTCAATCACCCATTCGGTCAGATTttgttgcgc                               >  2:185874/1‑248 (MQ=255)                                                                                                                                                                                                         gtgCGTTGTTTCAATATGCTGGTGGCGGTCTGCTCTCCCGGTACGATGTCGCTTTCGCGGTACACCGAGCA‑GATTTTTTCCGCTGGTAATCCCAGCGAACGACGCGATACAGCCTCAGGTAGTGCATCCACCACCTGATTGCAGACCGCCCACCAGGATAATTCAGCCAAAGATAATTCCCGCTCCTGCGTACCGCTTATTGCGTGACGGA‑TGACGTCAATCACCCATGCTGTCAGATTTTGTTGagca                              >  1:97676/1‑249 (MQ=255)                                                                                                                                                                                                                                   ggTCTGCTCTCCCGGTACGATGTCGCTTTCGCGGTACACCGAGCA‑GATTTTTTCCGCTGGTAATCCCAGCGAACGACGCGATACAGCCTCAGGTAGTGCATCCACCACCTGATTGCAGACCGCCCACCAGGATAATTCAGCCAAAGATAATTCCCGCTCCTGCGTACCGCTTATTGCGTGACGGA‑TGACGTCAATCACCCATGCTGTCAGATTTTGTTGAGCAAGCAGCTCCAGTGATTCCGATGTCTGGt  <  1:1130/251‑1 (MQ=255)                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                         GATCGTCGGCTGGCATACCGCAGCAAGCACACGGCTGTGTCTTAACCCAACGTGTGTATTTCTCCTTAACCCAGCGGCGACGTTTAGGCAGCTTCATGAAAGATTCCGGAGACTCTGGATCAACGGTGATGCTTACCACCGTCTTTTCCTGTGGTGATTTTTGTTGCTGGTGGGCGTAAGGCAACGGTGCAAGATTTTTTGTGCGTTGTTTCAATATGCTGGTGGCGGTCAGCTCTCCCGGTACGATGTCGCTTTCGCGGTACACCGAGCA‑GATTTTTTCCGCTGGTAATCCCAGCGAACGACGCGATACAGCCTCAGGTAGTGCATCCACCACCTGATTGCAGACCGCCCACCAGGATAATTCAGCCAAAGATAATTCCCGCTCCTGCGTACCGCTTATTGCGTGACGGA‑TGACGTCAATCACCCATGCTGTCAGATTTTGTTGAGCAAGCAGCTCCAGTGATTCCGATGTCTGGT  >  NC_000913/1643449‑1643925

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑