Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,350,005	C→T	V35I (GTC→ATC)	yciW ←	putative peroxidase YciW

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,350,005	0	C	T	94.1%	47.3 / NA	17	V35I (GTC→ATC)	yciW	putative peroxidase YciW
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (10/6);  minor base G (0/1);  total (10/7)
Fisher's exact test for biased strand distribution p-value = 4.12e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTAAGTCGCGCCGAATAGTAGTTACATAACCGCTGGACGCCGCAAACCTGAGCAACCGTCAGGGCCGTCGACAAGCGTTCATAAGCGGTGAAGGTCTGTGAACGGGTCACGGTAAGCTGGTCAGGGAACAAATCCACTGCAAGAACACGCGCAGGGTCCAGCCATGAAAGGAAGGGGGCCAGCGTTTCTTTTTCAAGGATCACGTCGAGTAGAAAGGGATCGCTGACCTTTGCGGCTTCCGGGACCAGAGGCAGAACGTCATACTCCGTAGTACTGGATTGCGTTTCATGATACCAGTGGCTTTTGCCGGTGATGTGGCGTTGTTCCATGGGCGCTCCTTGGTCGTAAAGGAAATCGTTATCCTGACGCAAGGCGGGAAGGGGAGAAAGACGGATCGGCGATAACAAATATCAGAAAGGTATAACAGAGATAACGGGCGGCAGAACGCCGCCCAT  >  NC_000913/1349779‑1350233                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                     gTAAGTCGCGCCGAATAGTAGTTACATAACCGCTGGACGCCGCAAACCTGAGCAACCGTCAGGGCCGTCGACAAGCGTTCATAAGCGGTGAAGGTCTGTGAACGGGTCACGGTAAGCTGGTCAGGGAACAAATCCACTGCAAGAACACGCGCAGGGTCCAGCCATGAAAGGAAGGGGGCCAGCGTTTCTTTTTCAAGGATCACGTAGAGTAGAAAGGGATCGCTGATCTTTGCGGCTTCCGGGACCAGAgg                                                                                                                                                                                                              >  1:246951/1‑251 (MQ=255)                              ccGCTGGACGCCGCAAACCTGAGCAACCGTCAGGGCCGTCGACAGGCGTTCATAAGCGGTGCAGGTCTGTGAACGGGTCACGGTAAGCTGGTCAGGGAACAAATCCACTGCAAGCACACGCGCAGGGTCCAGCCATGAAAGGAAGGGGGCCAGCGTTTCTTTTTCAAGGATCACGTCGAGTCGACAGGGATCGCTGTTCTTTGCGGCTTCCGGGACCAGAGGCAGAACGTCATACTCCGTAGTACTGGAtt                                                                                                                                                                                 >  2:57912/1‑251 (MQ=255)                                                                                                 tgtgAACGGGTCACGGTAAGCTGGTCAGGGAACAAATCCACTGCAAGAACACGCGCAGGGTCCAGCCATGAAAGGAAGGGGGCCAGCGTTTCTTTTTCAAGGATCACGTCGAGTAGAAAGGGATCGCTGATCTTTGCGGCTTCCGGGACCAGAGGCAGAACGTCATACTCCGTAGTACTGGATTGCGTTTCATGATACCAGTGGCTTTTGCCGGTGATGTGGCGTTGTTCCATGGGCGCTCCTTGGTCGt                                                                                                               >  2:47132/1‑250 (MQ=255)                                                                                                  gtgAACGGGTCACGGTAAGCTGGTCAGGGAACAAATCCACTGCAAGAACACGCGCAGGGTCCAGCCATGAAAGGAAGGGGGCCAGCGTTTCTTTTTCAAGGATCACGTCGAGTAGAAAGGGATCGCTGATCTTTGCGGCTTCCGGGACCAGAGGCAGAACGTCATACTCCGTAGTACTGGATTGCGTTTCATGATACCAGTGGCTTTTGCCGGTGATGTGGCGTTGTTCCATGGGCGCTCCTTGGTCGTa                                                                                                              >  1:51991/1‑250 (MQ=255)                                                                                                                   aGCTGGTCAGGGAACAAATCCACTGCAAGAACACGCGCAGGGTCCAGCCATGAAAGGAAGGGGGCCAGCGTTTCTTTTTCAAGGATCACGTCGAGTAGAAAGGGATCGCTGATCTTTGCGGCTTCCGGGACCAGAGGCAGAACGTCATACTCCGTAGTACTGGATTGCGGTTCATGATGCCAGTGGCTTTTGCCGGTGATGTGGCGTTGTTCCATGGGCGCTCCTTGGTCGTAAAGGAAATCGTTATCCTg                                                                                            <  1:125424/251‑1 (MQ=255)                                                                                                                    gCTGGTCAGGGAACAAATCCACTGCAAGAACACGCGCAGGGTCCAGCCATGAAAGGAAGGGGGCCAGCGTTTCTTTTTCAAGGATCACGTCGAGTAGAAAGGGATCGCTGATCTTTGCGGCTTCCGGGACCAGAGGCAGAACGTCATACTCCGTAGTACTGGATTGCGTTTCATGATACCAGTGGCTTTTGCCGGTGATGTGGCGTTGTTCCATGGGCGCTCCTTGGTCGTAAAGGAAATCGTTATCCTgg                                                                                           >  2:44533/1‑250 (MQ=255)                                                                                                                             ggAACAAATCCACTGCAAGAACACGCGCAGGGTCCAGCCATGAAAGGAAGGGGGCCAGCGTTTCTTTTTCAAGGATCACGTCGAGTAGAAAGGGATCGCTGATCTTTGCGGCTTCCGGGACCAGAGGCAGAACGTCATACTCCGTAGTACTGGATTGCGTTTCATGATACCAGTGGCTTTTGCCGGTGATGTGGCGTTGTTCCATGGGCGCTCCTTGGTCGTAAAGGAAATCGTTATCCTGACGCAAGGCg                                                                                  <  1:30677/251‑1 (MQ=255)                                                                                                                                    aTCCACTGCAAGAACACGCGCAGGGTCCAGCCATGAAAGGAAGGGGGCCAGCGTTTCTTTTTCAAGGATCACGTCGAGTAGAAAGGGATCGCTGATCTTTGCGGCTTCCGGGACCAGATGCAGAACGTCATACTCCGTAGTACTGGATTGCGTTTCATGATACCAGTGGCTTTTGCCGGTGATGTGGCGTTGTTCCATGGGCGCTCCTTGGTCGTAAAGGAAATCGTTATCCTGACGCAAGGCGGGAAggg                                                                           <  1:44533/251‑1 (MQ=255)                                                                                                                                                    cgcgCAGGGTCCAGCCATGAAAGGAAGGGGGCCAGCGTTTCTTTTTCAAGGATCACGTCGAGTAGAAAGGGATCGCTGATCTTTGCGGCTTCCGGGACCAGAGGCAGAACGTCATACTCCGTAGTACTGGATTGCGTTTCATGATACCAGTGGCTTTTGCCGGTGATGTGGCGTTGTTCCATGGGCGCTCCTTGGTCGTAAAGGAAATCGTTATCCTGACGCAAGGCGGGAAGGGGAGAAAGACGGATCgg                                                           <  1:211504/251‑1 (MQ=255)                                                                                                                                                       gcAGGGTCCAGCCATGCACGGAAGGGGGACAGCGTTTCTTTTTCAAGGATCAAGTCGAGAAGAAAGGGATCGCTGATCTTTGCGGCTTCCGGGCCCAGAGGCAGAACGTCATACTCCGTCGTACTGGATTGCGTTTCATGATACCAGTGGCTTTTGCCGGTGCTGTGGCGTTGTTCCATGGGCGCTCCTTGGTCGTAAAGGAAATCGTTATCCTGACGCAAGGCGGGAAGGGGAGAAGTACGGATCGGCg                                                         >  2:101893/1‑250 (MQ=255)                                                                                                                                                          gggTCCAGCCATGAAAGGAAGGGTGCCAGCGTTTCTTTTTCAAGGATCACGTCGAGGAGAAAGGGATCGCTGATCGTTGCGGCTTCCGGGACCAGAGGCAGAACGTCATACTCCGTAGTACTGGAGTGCGTTTCATGATACCAGTGGCTGTTGCCGGTGATTTGGCGTTGTTCCAAGGGCGCTCCTTGGTAGTAAAGTAAATCGTGAGCCTGACGCAAGTAGGGAAGGGGAGAAAGACGGATCGGCGATaa                                                     <  2:254381/251‑1 (MQ=255)                                                                                                                                                                      gAAAGGAAGGGGGCCAGCGTTTCTTTTTCAAGGATCACGTCGAGTAGCAAGGGATCGCTGATCTTTGCGGCTTCCGGGACCAGAGGAAGAACGTCATACTCCGTAGTACTGGATTGCGTTTCATGATACCAGTGGCTATTGCCGGTGCTGTGGCGTTGTTCCATGGGCGCTCCTTGGTCGTAAAGGAAATCGTTATCCTGACGCAAGGCGGGAAGGGGAGAAAGACGGATCGGCGATAACAAATATCAGa                                          >  2:253770/1‑250 (MQ=255)                                                                                                                                                                                       cGTTTCTTTTTCAAGGATCACGTCGAGTAGACAGGGATCGCTGATCTTTGCGGCTTCCGGGACCAGAGGCAGAACGTCATACTCCGTAGTACTGGATTGCGTTTCATGATACCAGTGGCTTTTGCCGGTGATGTGGCGTTGTTCCATGGGCGCTCCTTGGTCGTAAAGGAAATCGTTATCCTGACGCAAGGCGGGAAGGGGAGAAAGACGGATCGGCGATAACAAATATCAGAAAGGTATAACAGAGATa                         >  1:215664/1‑250 (MQ=255)                                                                                                                                                                                                     ggagcACGTCGAGTAGAAAGGGATCGCTGATCTTTGCGGCTTCCGGGACCAGAGGCAGAACGTCATACTCCGTAGTACTGGATTGCGTTTCATGATACCAGTGGCTTTTGCCGGTGATGTGGCGTTGTTCCATGGGCGCTCCTTGGTCGTAAAGGAAATCGTTATCCTGACGCAAGGCGGGAAGGGGAGAAAGACGGATCGGCGATAACAAATATCAGAAAGGt                                     <  2:245722/220‑1 (MQ=255)                                                                                                                                                                                                     ggATCACGTCGAGTAGAAAGGGATCGCTGATCTTTGCGGCTTCCGGGACCAGAGGCAGAACGTCATACTCCGTAGTACTGGATTGCGTTTCATGATACCAGTGGCTTTTGCCGGTGATGTGGCGTTGTTCCATGGGCGCTCCTTGGTCGTAAAGGAAATCGTTATCCTGACGCAAGGCGGGAAGGGGAGAAAGACGGATCGGCGATAACAAATATCAGAAAGGt                                     >  1:245722/1‑224 (MQ=255)                                                                                                                                                                                                      gATCACGTCGAGTAGAAAGGGATCGCTGATCTTTGCGGCTTCCGGGACCAGAGGCAGAACGTCATACTCCGTAGTACTGGATTGCGTTTCATGATACCAGTGGCTTTTGCCGGTGATGTGGCGTTGTTCCATGGGCGCTCCTTGGTCGTAAAGGAAATCGTTATCCTGACGCAAGGCGGGAAGGGGAGAAAGACGGATCGGCGATAACAAATATCAGAAAGGTATAACAGAGATAACGGGCGGCAGAAcgc         >  1:10826/1‑251 (MQ=255)                                                                                                                                                                                                             gcGAGGAGAAATGGAGCGCTGAGCTTTGCGGCTTCCGGGACCAGAGGCAGAACGTCATACTCCGTAGTACTGGATTGCGTTTCATGATACCAGTGGCTTTTGCCGGTGATGTGGCGTTGTTCCATGGGCGCTCCTTGGTCGTAAAGGAAATCGTTATCCTGACGCAAGGCGGGAAGGGGAGAAAGACGGATCGGCGATAACAAATATCAGAAAGGTATAACAGAGATAACGGGCGGCAGAACGCCGCCCAt  <  1:253770/250‑1 (MQ=255)                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                     GTAAGTCGCGCCGAATAGTAGTTACATAACCGCTGGACGCCGCAAACCTGAGCAACCGTCAGGGCCGTCGACAAGCGTTCATAAGCGGTGAAGGTCTGTGAACGGGTCACGGTAAGCTGGTCAGGGAACAAATCCACTGCAAGAACACGCGCAGGGTCCAGCCATGAAAGGAAGGGGGCCAGCGTTTCTTTTTCAAGGATCACGTCGAGTAGAAAGGGATCGCTGACCTTTGCGGCTTCCGGGACCAGAGGCAGAACGTCATACTCCGTAGTACTGGATTGCGTTTCATGATACCAGTGGCTTTTGCCGGTGATGTGGCGTTGTTCCATGGGCGCTCCTTGGTCGTAAAGGAAATCGTTATCCTGACGCAAGGCGGGAAGGGGAGAAAGACGGATCGGCGATAACAAATATCAGAAAGGTATAACAGAGATAACGGGCGGCAGAACGCCGCCCAT  >  NC_000913/1349779‑1350233

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑