Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,336,674	G→A	G282R (GGG→AGG)	acnA →	aconitate hydratase 1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,336,674	0	G	A	100.0%	39.8 / NA	14	G282R (GGG→AGG)	acnA	aconitate hydratase 1
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (7/7);  total (7/7)

CACACCACCATGATCAACGGCCTTGGCGTGCTGGGGTGGGGCGTTGGTGGGATCGAAGCAGAAGCCGCAATGTTAGGCCAGCCGGTTTCCAT‑GCTTATCCCGGATGTAGTGGGCTTCAAACTTACCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTGATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGTTTAAGCGGGCGCAGCGAAGATCAGGTCGAGTTGGTCGAAAAATATGCCAAAGCGCAGGGCATGTGGCGTAACCCGGGCGATGAACCAATTTTTACCAGT  >  NC_000913/1336470‑1336913                                                                                                                                                                                                              |                                                                                                                                                                                                                                                cccaccacCAGGATCAACGGTCGGGGGTTGCTGGGGGGGGGGGTGGGGGTGAGCGAAGAATAAGCCGCAATGTTAGGCCCGCCGGTT‑CCATTGCTTACCCCGGATGTATGGGGCTTCAACCTTACCGGAAACTTCCGTGAAGGAATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGAGGAAATTCGTCGAATTTTATGGTGATGGTCTGGATTCACTACCGtt                                                                                                                                                                                                   <  2:204972/249‑1 (MQ=255)                              ggtggggtggggGGTTGGTGGGATCGAAGCAAAAGCCGAAAGGTTGGGCAAGCCGGTTTCCAT‑GCTTATCCTGGATGTAGTGGGCTTAAAACTTACCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGAGGAAATTCGTCGAATTTTATGGTGATGTTCTGGATTCAATACCGTTGGCGGATCGCGCCACCATTGCCAATATGt                                                                                                                                                                      <  1:263727/249‑1 (MQ=255)                                                       gAAGCAGAAGCCGCACTGTTAGGCCAGCCGGTTTCCAT‑GCTTATCCCGGATGTAGTGGGCTTCAAACTTACCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGAGGAAATTCGTCGCAtt                                                                                                                                                                                                                                 >  2:20603/1‑167 (MQ=255)                                                       gAAGCAGAAGCCGCAATGTTAGGCCAGCCGGTTTCCAT‑GCTTATCCCGGAGGTAGTGGGCTTCAAACTTACCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGAGGAAATTCGTCGAAtt                                                                                                                                                                                                                                 <  1:20603/167‑1 (MQ=255)                                                        acacAGAAGCCGCAAGTTTGGGCCAGCCGGTTTCCTT‑GCTGATCCCGGATGTTGTGGGCTTCAAACTTACCGAAAATTTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGAGGAAATTCGTCGAATTTTAGGGTGATGGTCTGGATTCACTCCCGTTGGCGGATCGCGCCCCCATTGCCAATATGGCGCCAGAATATGGTGCCACCTGTGGc                                                                                                                                            <  2:51118/248‑1 (MQ=255)                                                                           aGGCCAGCCGGTTTCCAT‑GATTATCCCGGATGTAGTGGGCTTCAAACTTACCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGAGGAAATTCGTCGGATTTTATGTTGATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCCCCTTTGGCTTCTTCCCAATCGATTCTg                                                                                                                         >  2:16142/1‑251 (MQ=255)                                                                           aGGCCAGCAGGTTTCCAT‑GCTTATCCCGGATGTAGTGGCCTTCAAACTTACCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGATCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGAGGAAATTCGTCGAATTTTATGGTGATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTg                                                                                                                         >  2:51855/1‑251 (MQ=255)                                                                              ccAGCCGGTTTCCAT‑GCTTATCCCGGATGTAGTGGGCTTCAAACTTACCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGAGGAAATTCGTCGAATTTTATGGTGATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTa                                                                                                                       >  1:287/1‑250 (MQ=255)                                                                                         ccAT‑GCTTATCCCGGATGTAGTGGGCTTCAAACTTACCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGAGGCAATTCGTCGAATTTTATGGTGATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTAc                                                                                                           >  2:191763/1‑251 (MQ=255)                                                                                                                                                    tACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGAGGAAATTCGTCGAATTTTATGGTGATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAAGCGATGCTGTAACCCTCGATTACATGCGTTTAAGCGGGCGCAGCGAAGATCAGGTCGAGTTGGTCGAAAAATATGCCaaa                                                  <  1:92117/250‑1 (MQ=255)                                                                                                                                                         ccACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTTGTGAGGAAATTCGTCGAATTTTATGGTGATGGTCTGGATTCACTACCGTTGGTGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGTTTAAGCGGGCGCAGCGAAGATCAGGTCGAGTTGGTCGAAAAATATGCCAAAGCGCAg                                            <  1:52315/251‑1 (MQ=255)                                                                                                                                                         ccACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGAGGAAATTCGTCGAATTTTATGGTGATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGTTTAAGCGGGCGCAGCGAAGATCAGGTCGAGTTGGTCGAAAAATATGCCAAAgcgc                                              >  2:161332/1‑249 (MQ=255)                                                                                                                                                                                               aaaCATGGCGTGGTGAGGAAATTCGTCGAATTTTATGGTGATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGTTTAAGCGGGCGCAGCGAAGATCAGGTCGAGTTGGTCGAAAAATATGCCAAAGCGCAGGGCATGTGGCGTAACCCGGGCGATGAACCAATTTTTAc      >  1:85982/1‑251 (MQ=255)                                                                                                                                                                                                   acggCGTGGTGAGGAAATTCGTCGAATTTTATGGTGATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATGACATGCGTTTAAGCGGGCGCAGCGAAGAGCAGGTCGAGTTGGTCGAAAAATATGCCAAAGCGCAGGGCATGTGGCGTAACCCGGGCGATGAACCAATTTTTACCAGt  <  1:251020/249‑1 (MQ=255)                                                                                                                                                                                                              |                                                                                                                                                                                                                                                CACACCACCATGATCAACGGCCTTGGCGTGCTGGGGTGGGGCGTTGGTGGGATCGAAGCAGAAGCCGCAATGTTAGGCCAGCCGGTTTCCAT‑GCTTATCCCGGATGTAGTGGGCTTCAAACTTACCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTGATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGTTTAAGCGGGCGCAGCGAAGATCAGGTCGAGTTGGTCGAAAAATATGCCAAAGCGCAGGGCATGTGGCGTAACCCGGGCGATGAACCAATTTTTACCAGT  >  NC_000913/1336470‑1336913

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑