Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,287,040	G→A	intergenic (+56/+26)	ychS → / ← rttR	putative uncharacterized protein YchS/small RNA RttR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,287,040	0	G	A	100.0%	16.5 / NA	7	intergenic (+56/+26)	ychS/rttR	putative uncharacterized protein YchS/small RNA RttR
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (4/3);  total (4/3)

CGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGC  >  NC_000913/1286920‑1287286                                                                                                                         |                                                                                                                                                                                                                                                       cgcTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTAAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCt                                                                                                                      >  1:82806/1‑251 (MQ=255)  gcTTCGCCCTTCGGGTCGTTGCCTGAGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGTTTCTCACCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTAAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCATCGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCtt                                                                                                                     <  1:171631/251‑1 (MQ=255)                  cGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTAAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGCGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGc                                                                                                      >  1:25232/1‑250 (MQ=255)                                   ctctctCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTAAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTGGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAAACTTGGTAGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAAt                                                                                    >  1:96446/1‑251 (MQ=255)                                       ctctctgccGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGTATGGGCAGAATATTTGATTGCGGATGCGCTTAAGAGTTCAGGTACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTTGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAAtt                                                                                   <  1:229297/240‑1 (MQ=17)                                                               tAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTAAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCATCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGAc                                                         <  2:82806/250‑1 (MQ=255)                                                                                                                     gCTTAAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCCGCACATTTAATTGCGGATTCGTTGGGAAGTTCAGGGGCTTTTGAAAGTGGTGGTGGTGGGGGGTGGATACGCACCTTCGAAGTGGAAGACggg  >  2:156101/1‑250 (MQ=17)                                                                                                                         |                                                                                                                                                                                                                                                       CGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGC  >  NC_000913/1286920‑1287286

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑