Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,169,836	A→G	L180P (CTG→CCG)	ldtC ←	L,D‑transpeptidase LdtC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,169,836	0	A	G	100.0%	22.9 / NA	8	L180P (CTG→CCG)	ldtC	L,D‑transpeptidase LdtC
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (3/5);  total (3/5)

TTATCGGAGTGTTGATGATATTCACTTTGGTGCCTGGGGTGACCTGGCTAAAGAGTGTTTTGATATCGTCATCCCGCAGACGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATCGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGTTATCCAGTCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGTTGGCGTCCAGGTTGGGTTTGCACGTTTGTCTGAAACGGTGGTCACCATTGTCGGTGTCAGCGTGTCACCACCTAACTGACCAATACCTATTGGATACACGGTTACCGAATTTTTACCCGGCGGGTAGTAATAGAGACGCAGCTCCGCAATGTTGATCACAATGCCTTCGCGCGGC  >  NC_000913/1169639‑1170078                                                                                                                                                                                                      |                                                                                                                                                                                                                                                   ttATCGGAGTGTTGACGATATTCACTTTGGTGCCTGGGGTGACCTGGCTAAATAGTGTTTTGATATCGGCATCCCGCAGACGAATTCAGCCAGAACTTACCCGCATGCCAATGCCGAAATCGGCTTTCGTACCATGAAGAAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGTTATCCGGTCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGgc                                                                                                                                                                                                <  2:52570/250‑1 (MQ=255)                  atagtCACGTTGGTGACTGGGGGGACCTGGCTAAAGAGTGTTTTGATATCGTCATCCCGAAGACGAATAAAGCCAGAACTGAACCGCATGCCAATGCCGAAATCGGCGTTCGTACCATGAAGCAAAGAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCATGGGGTTATCCGGTGCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTACAACGGGCGCGGATGTTTGCCGTTgg                                                                                                                                                                              <  1:103152/247‑1 (MQ=255)                                                         gtTTTGATATCGTCATCCCGCAGACGAATACCGCCAGCACTTACCCGCATGCCAATGCCGAAATCGTCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGTTATCCGGTCCAGCCGTCACTACCGCAGGCCACTCAATTCCCTGTGCTTTATCACGGGCGAGGATTGTTGCCGTTGACGACCAGGTTGGGTTTGCACGTTTGTCTTTAACggtggt                                                                                                                                       >  1:53587/1‑251 (MQ=255)                                                                   cGTCATCCCGCAGACAAAGACAGCCCAATCTTGCCCGCATGCCAATGCCGAAATCGGCGTTCGTTCCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGTTATCCGGTCCAGCCGGCACTACCGCAGGCAACTCAATGCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGTTGGCGTCCAGGTTGGGTTTGCACGTTTGGCTGAAACGGTGGTCACTATTGTc                                                                                                                             <  2:101217/251‑1 (MQ=255)                                                                                                                    gAAATCGGCGTTCGTACCATGAAGCAAATACACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGTTATCCGGTCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGTTGGCGTCCAGGTTGGGTTTGCACGTTTGTCTGAAACGGTGGTCACCATTGTCGGTGTCAGCGTGTCACCACCTAACTGACCAATACCTATTGGATACACgg                                                                            >  2:173453/1‑251 (MQ=255)                                                                                                                                                 aaaCGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGTTATCCGGTCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGTTGGCGTCCAGGTTGGGTTTGCACGTTTGTCTGAAACGGTGGTCACCATTGTCGGTGTCAGCGTGTCACCACCTAACTGACCAATACCTATTGGATACACGGTTACCGAATTTTTACCCGGCGGgtagta                                                <  1:194264/250‑1 (MQ=255)                                                                                                                                                       cgccATAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGTTATCCGGTCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGTTGGCGTCCAGGTTGGGTTTGCACGTTTGTCTGAAACGGTGGTCACCATTGTCGGTGTCAGCGTGTCACCACCTAACTGACCAATACCTATTGGATACACGGTTACCGAATTTTTACCCGGCGGGTAGTAATag                                            >  1:216407/1‑248 (MQ=255)                                                                                                                                                                                              ggTTATCCGGTCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGTTGGCGTCCAGGTTGGGTTTGCACGTTTGTCTGAAACGGTGGTCACCATTGTCGGTGTCAGCGTGTCACCACCTAACTGACCAATACCTATTGGATACACGGTTACCGAATTTTTACCCGGCGGGTAGTAATAGAGACGCAGCTCCGCAATGTTGATCACAATGCCTTCGCgcggc  <  2:165069/251‑1 (MQ=255)                                                                                                                                                                                                      |                                                                                                                                                                                                                                                   TTATCGGAGTGTTGATGATATTCACTTTGGTGCCTGGGGTGACCTGGCTAAAGAGTGTTTTGATATCGTCATCCCGCAGACGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATCGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCATGATGGCCCATTGGGTTATCCAGTCCAGCCGGCACTACCGCAGGCAACTCAATTCCCTGTGCTTTATAACGGGCGCGGATGTTTGCCGTTGGCGTCCAGGTTGGGTTTGCACGTTTGTCTGAAACGGTGGTCACCATTGTCGGTGTCAGCGTGTCACCACCTAACTGACCAATACCTATTGGATACACGGTTACCGAATTTTTACCCGGCGGGTAGTAATAGAGACGCAGCTCCGCAATGTTGATCACAATGCCTTCGCGCGGC  >  NC_000913/1169639‑1170078

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑