Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,148,972 C→T Q72* (CAG→TAG)  fabH → 3‑oxoacyl‑[acyl carrier protein] synthase 3

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,148,9720CT93.3% 32.7 / ‑5.2 15Q72* (CAG→TAG) fabH3‑oxoacyl‑[acyl carrier protein] synthase 3
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (6/8);  minor base G (0/1);  total (6/9)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.59e-01

ACGGTATATAACCGAAAAGTGACTGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGACAAACGCCGATTTGGAAAAAATGGTGGACACCTCTGACGAGTGGATTGTCACTCGTACCGGTATCCGCGAACGCCACATTGCCGCGCCAAACGAAACCGTTTCAACCATGGGCTTTGAAGCGGCGACACGCGCAATTGAGATGGCGGGCATTGAGAAAGACCAGATTGGCCTGATCGTTGTGGCAACGACTTCTGCTACGCACGCTTTCCCGAGCGCAGCTTGTCAGATTCAAAGCATGTTGGGCATTAAAGGTTGCCCGGCATTTGACGTTGCAGCAGCCTGCGCAGGTTTCACCTATGCATTAAGCGTAGCCGATCAATACGTGAAATCTGGGGCGGTGAAGTATGCTCTGGTCGTCGGTTCCGATGTACTGGCGCGCACCTGCGATCCAA  >  NC_000913/1148727‑1149203
                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                       
aCGGTATATAACCGAAAAGTGACTGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGACAAACGCCGATTTGGAAAAAATGGTGGACATCTCTGACGAGTGGATTGTCACTCGTACCGGTATCCGCGAACGCCACATTGCCGCGCCAAACGAAACCGTTTCAACCATGGGCTTTGAAGCGGCGACACGCGCAATTGAGATGGCGGGCATTGAGAAAGACTAGAtt                                                                                                                                                                                                                                    <  1:10453/251‑1 (MQ=255)
  ggaatagaaacGAAAATTGACTGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCTAACAAGGGCGGACAAACGCCGATTTGGAAAAAATGGTGGACTCCTCTGACGAGTGGATTGTCACTCGTACCGGAATCCGCGAACGCCACATTGCCGCGCCAAACGAAACCGTTTAAACCATGGGCTGTGAAGCGGCGACACGCGCAATTGAGATGGCGTGCATTGAGAAAGTCTAGATTgg                                                                                                                                                                                                                                  <  2:198564/241‑1 (MQ=255)
            cGAAAAGGGACTGAGCGTACATGTATACGAAGAGTATTGGTACTGGCAACTATCTACCCTAACAAGTGCGGACAAACGCCGATTTGGAAAAAATGGTGGACTCCTCTGCCGAGTGGATTGACATCCGTATCGGTTTCCGCGATCGCCACGTTGCCGCGCGAAACGAAACAGTTTCAACCTTGGTCTGTGATGCGGCGGCACCCGCTGTTGAGATGTCGGACATTGAGAAACACGATAATGGTCTGATCGtt                                                                                                                                                                                                                        <  2:16191/251‑1 (MQ=255)
                   tGACTGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGACAAACGCCGATTTTGAAAAAATGGTGGACACCTCTGACGAGTGGATTGTCACTCGTACCGGTATCCGCGAACGCCACATTGCCGCGCCAAACGAAACCGTTTCAACCATGGGCTTTGAAGCGGCGACACGCGCAATTGAGATGGCGGGCATTGAGAAAGACTAGATTGGCCTGATCGTTGTGGCa                                                                                                                                                                                                                  >  1:70569/1‑250 (MQ=255)
                                         aaGATTATTGGTACTGGCAGCTATCTGCCCGCACAAGTGCGGACAAACGCCGATTTGGAAAAAATGGTGGACACCTCTGACGAGTGGATTGTCACTCGTACCGGTATCCGCGAACGCCACATTGCCGCGCCAAACGAAACCGTTTCAACCATGGGCTTTGAAGCGGCGACACGCGCAATTGAGATGGCGGGCATTGAGAAAGACTAGATTGGCCTGATCGTTGGGGCACCGACTTCTGCTACGCACGCttt                                                                                                                                                                                           >  2:168227/1‑251 (MQ=255)
                                                        ggCAGCTATCTGCCCGAACAAGTGCGGACAAACGCCGATTTGGAAAAAATGGTGGACACCTCTGACGAGTGGATTGTCACTCGTACCGGTATCCGCGAACGCCACATTGCCGCGCCAAACGAAACCGTTTCAACCATGGGCTTTGAAGCGGCGACACGCGCAATTGAGATGGCGGGCATTGAGAAAGACTAGATTGGCCTGATCGTTGTGGCAACGACTTCTGCTACGCACGCTTTCCCGAGCGCAGCTTg                                                                                                                                                                            <  2:203691/251‑1 (MQ=255)
                                                                   gCCCGAACAAGTGCGGACAAACGCCGATTTGGAAAAAATGGTGGACACCGCTGACGATTGGATTGCCACTCGTACCGGTATCCGCGAACGCCACATGGCCGCGCCAAACGAAACCGTTGCAACCATGGGCTTTGAAGCGGCGACACGCGCAATTGAGATGGCGGGCATTGAGAAAGACTAGATTGGCCTGATCGTTGTGGCAACGACGTCTGCTACGCACGCTTTCCCGAGCGCAGCTTGTCAGAtt                                                                                                                                                                     <  2:177563/247‑1 (MQ=255)
                                                                                     aaaCGCCGATTTGGAAAAAATGGTGGACACCTCTGACGAGTGGATTGTCACTCGTACCGGTCTCCGCGAACGCCACATTGCCGCGCCAAACGAAACCGTTTCAACCATGGGCTTTGAAGCGGCGACACGCGCAATTGAGATGGCGGGCATTGAGAAAGACTAGATTGGCCTGATCGTTGTGGCAACGACTTCTGCTACGCACGCTTTCCCGAGCGCAGCTTGTCAGATTCAAAGCATGTTGGGCATTaa                                                                                                                                                 >  2:47990/1‑249 (MQ=255)
                                                                                              tttGGAAAAAATGGTGGACACCTCTGACGAGTGGATTGTCACTCGTACCGGTATCCGCGAACGCCACATTGCCGCGCCAAACGAAACCGTTTCAACCATGGGCTTTGAAGCGGCGACACGCGCAATTGAGATGGCGGGCATTGAGAAAGACTAGATTGGCCTGATCGTTGTGGCAACGACTTCTGCTACGCACGCTTTCCCGAGCGCAGCTTGTCAGATTCAAAGCATGTTGGGCATTAAAGGTTGCCCgg                                                                                                                                      >  1:120597/1‑251 (MQ=255)
                                                                                                   aaaaaaTGGTGGACACCTCTGACGAGTGGATTGTCACTCGTACCGGTATCCGCGAACGCCACATTGCCGCGCCAAACGAAACCGTTTCAACCATGGGCTTTGAAGCGGCGACACGCGCAATGGAGATGGCGGGCATTGAGAAAGACTAGATTGGCCTGATCGTTGTGGCAACGACTTCTGCTACGCACGCTTTCCCGAGCGCAGCTTGTCAGATTCAAAGCATGTTGGGCATTAAAGGTTGCCCGGCAttt                                                                                                                                 <  1:157276/251‑1 (MQ=255)
                                                                                                                       gACGAGTGGATTGTCACTCGTACCGGTATCCGCGAACGCCACATGGCCGCGCCAAACGAAACCGTTTCAACCATGGGCTTTGAAGCGGCGACACTCGCAATTGAGATGGCGGGCATTGAGAAAGACTAGATTGGCCTGAGCGTTGTGGCAACGACTTCTGCTACGCACGCTTTCCCGAGCGCAGGTTGTCAGATTCAATGCATGTTGGTCATTAAAGGGGGCCCGGCATTCGACGGTGCAGCAGCCTgcgc                                                                                                             <  2:27796/251‑1 (MQ=255)
                                                                                                                                                                                gAAACCGTTTCAACCATGGGCTTTGAAGCGGCGACACGCGCAATTGAGATGGCGGGCATTGAGAAAGACTAGATTGGCCTGATCGTTGTGGCAACGACTTCTGCTACGCACGCTTTCCCGAGCGCAGCTTGTCAGATTCAAAGCATGTTGGGCATTAAAGGTTGCCCGGCATTTGACGTTGCAGCAGCCTGCGCAGGTTTCACCTATGCATTAAGCGTAGCCGATCAATACGTGAAATCTGGGGAGGTGa                                                     >  1:194393/1‑250 (MQ=255)
                                                                                                                                                                                                    cTTTGAAGCGGCGACACGCGCAATTGAGATGGCGGGCATTGAGAAAGACTAGATTGGCCTGATCGTTGTGGCAACGACTTCTGCTACGCACGCTTTCCCGAGCGCAGCTTGTCAGATTCAAAGCATGTTGGGCATTAAAGGTTGCCCGGCATTTGACGTTGCAGCAGCCTGCGCAGGTTTCACCTATGCATTAAGCGTAGCCGATCAATACGTGAAATCTGGGGCGGTGAAGTATGCTCTGGTCGTCGGtt                                >  1:207000/1‑251 (MQ=255)
                                                                                                                                                                                                               cgACACGCGCAATTGAGATGGCGGGCATTGAGAAAGTCTAGATTGGCCTGATCGTTGTGGCAACGACTTCTGCTACGCACGCTTTCCCGAGCGCAGCTTGTCAGATTCAAAGCATGTTGGGCATTAAAGGTTGCCCGGCATTTGACGTTGCAGCAGCCTGCGCAGGTTTCACCTAGGCATTAAGCGTAGCCGATCAATACGTGAAATCTGGGGGGGTGAAGTATGCTCGGGTCGTCGGTTCCGATGTACTg                     <  2:174762/251‑1 (MQ=255)
                                                                                                                                                                                                                                  ggCGGGCATTGAGAAAGACTAGATTGGCCTGATCGTTGTGGCAACGACTTCTGCTACGCACGCTTTCCCGAGCGCAGCTTGTCAGATTCAAAGCATGTTGGGCATTAAAGGTTGCCCGGCATTTGACGTTGCAGCAGCCTGCGCAGGTTTCACCTATGCATTAAGCGTAGCCGATCAATACGTGAAATCTGGGGCGGTGAAGTATGCTCTGGTCGTCGGTTCCGATGTACTGGCGCGCACCTGCGATCCaa  <  1:48024/251‑1 (MQ=255)
                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                       
ACGGTATATAACCGAAAAGTGACTGAGCGTACATGTATACGAAGATTATTGGTACTGGCAGCTATCTGCCCGAACAAGTGCGGACAAACGCCGATTTGGAAAAAATGGTGGACACCTCTGACGAGTGGATTGTCACTCGTACCGGTATCCGCGAACGCCACATTGCCGCGCCAAACGAAACCGTTTCAACCATGGGCTTTGAAGCGGCGACACGCGCAATTGAGATGGCGGGCATTGAGAAAGACCAGATTGGCCTGATCGTTGTGGCAACGACTTCTGCTACGCACGCTTTCCCGAGCGCAGCTTGTCAGATTCAAAGCATGTTGGGCATTAAAGGTTGCCCGGCATTTGACGTTGCAGCAGCCTGCGCAGGTTTCACCTATGCATTAAGCGTAGCCGATCAATACGTGAAATCTGGGGCGGTGAAGTATGCTCTGGTCGTCGGTTCCGATGTACTGGCGCGCACCTGCGATCCAA  >  NC_000913/1148727‑1149203

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑