Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	880,964	C→T	L80F (CTT→TTT)	dacC →	D‑alanyl‑D‑alanine carboxypeptidase DacC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	880,964	0	C	T	100.0%	54.5 / NA	18	L80F (CTT→TTT)	dacC	D‑alanyl‑D‑alanine carboxypeptidase DacC
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (8/10);  total (8/10)

CGTGGTCTTGCAGCGGGTTCTGCATTTTTATTCCTTTTTGCCCCAACGGCATTCGCGGCGGAACAAACCGTTGAAGCGCCGAGCGTGGATGCGCGTGCATGGATTTTAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCGATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGAACAAAGGTGTGATTATCCAGTCCGGTAATGACGCCTGTATTGCGCTGGCTGATTACGTTGCCGGGAGCCAGGAGTCATTTATTGGTCTGATGAATG  >  NC_000913/880751‑881195                                                                                                                                                                                                                      |                                                                                                                                                                                                                                        cGTGGTCTTGCAGCGGGTTCTGCATTTTTATTCCTTTTTGCCCCAACGGCATTCGCGGCGGAACAAACCGTTGAAGCGCCGAGCGTGGATGCGCGTGCATGGATTTTAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGTTTAAGGCCGATAAGATTAAACTCACCGATATGGTGAc                                                                                                                                                                                                    >  1:122197/1‑251 (MQ=255)               gggTTCTGCATTTTTATTCCTTTTTGCCCCACCGGCATTCGCGGCGGAACAAACCGTTGAAGCGCCGAGCGTGGATGCGCGTGCATGGATTTTAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGTTTAAGGCCGCTAAGATTAAACTCACCGATATGGTGACGGTCGGTAAAGATg                                                                                                                                                                                      >  1:198423/1‑251 (MQ=255)                                            cAACGGCATTCGCGGCGGAACAAACCGTTGAAGCGCCGAGCGTGGATGCGCGTGCATGGATTTTAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGTTTAAGGCCGATAAGATTAAACTCACCGATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGt                                                                                                                                                         >  1:249028/1‑251 (MQ=255)                                                         ggcggAACAAACCGTTGAAGCGCCGAGCGTGGATGCGCGTGCATGGATTTTAATGGATTACGCCAGCGGCAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGTTTAAGGCCGATAAGATTAAACTCACCGATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTa                                                                                                                                             >  2:108247/1‑250 (MQ=255)                                                              aaCAAACCGTTGAAGCGCCGAGCGGGGATGCGCGTGCATGGATTTTAATGGATTACGACAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACGGGATCCCGCGAGCCTGACTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGTTTAAGGCCGATAAGATTAAACTCACCGATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTTCGTGGTTCATCGGTAATGTTc                                                                                                                                       <  1:147923/251‑1 (MQ=255)                                                                          aaGCTCCGAGCGTGGATGCGCGGGCATGGATTTTAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGTTTAAGGCCGATAAGATTAAACTCACCGATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGc                                                                                                                           <  2:25912/251‑1 (MQ=255)                                                                                                 gCATGGATTTTAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGTTTAAGGCCGATAAGATTAAACTCACCGATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACt                                                                                                     <  1:31060/250‑1 (MQ=255)                                                                                                       aTTTTAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGTTTAAGGCCGATAAGATTAAACTCACCGATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGAACa                                                                                               >  1:143089/1‑250 (MQ=255)                                                                                                          ttAAGGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATGACCAGCTATGTGTTTGGGCAGGCGTTTAAGGCCGATAAGATTAAACTCACCGATATGGTGACGGTCGGTAAAGATGCTTGGGCGACGGGAAATCCGGCACTGTGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGAACAAAg                                                                                            <  2:207329/250‑1 (MQ=255)                                                                                                                     cGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGTTTAAGGCCGATAAGATTAAACTCACCGATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGAACAAAGGTGTGATTATcc                                                                                >  2:95922/1‑251 (MQ=255)                                                                                                                         aGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGTTTAAGGCCGATAAGATTAAACTCACCGATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGAACAAAGGTGTGATTATCCAGTc                                                                            <  2:72607/251‑1 (MQ=255)                                                                                                                          gCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCTCGAGCCTGACTAAAATCATGATCAGCTATGTGGTTGGGCAGGCGTTTAAGGCCGATAAGATTAAACTCACCGATATGGTGACGGTCTGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCTTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGAACAAAGGTGTGATTATCCAGTcc                                                                           <  1:12024/251‑1 (MQ=255)                                                                                                                                  gTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGTTTAAGGCCGATAAGATTAAACTCACCGATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGAACAAAGGTGTGATTATCCAGTCCGGTAATGa                                                                   <  2:6003/251‑1 (MQ=255)                                                                                                                                           gaaggaattGCGGATGAGAAGCTGTATCTCGTGTGCCTGTCTAAAATCATGACCAGCTATGTGGTTTGGCAGGAGTTGAAGGCCGATAAGATTAAACTCCCCGATAGGTTGACGGTAGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTTCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGAGCAGGGTTCGGTGGCAGACTGGAACAAAGGTGTGATTATCCAGTCCGGTAATGACTCCTGTAt                                                          <  2:69125/243‑1 (MQ=255)                                                                                                                                                              aaCTGGATCCCGCGAGCCTGACTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGTTTAAGGCCGATAAGATTAAACTCACCGATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGAACAAAGGTGTGATTATCCAGTCCGGTAATGACGCCTGTATTGCGCTGGCTGATTACGtt                                       <  1:118989/251‑1 (MQ=255)                                                                                                                                                                      cccGCGAGCCTGACTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGTTTAAGGCCGATAAGATTAAACTCACCGTTATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGAACAAAGGTGTGATTATCCAGTCCGGTAATGACGCCTGTATTGCGCTGGCTGATTACGTTGCCggg                                 >  1:225437/1‑249 (MQ=255)                                                                                                                                                                                     aaaaTCATGACCAGCTATGTGGTTGGGCAGGCGTTTAAGGCCGATAAGATTAAACTCACCGATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGAACAAAGGTGTGATTATCCAGTCCGGTAATGACGCCTGTATTGCGCTGGCTGATTACGTTGCCGGGAGCCAGGAGTCATTTa                 <  2:48530/250‑1 (MQ=255)                                                                                                                                                                                                   cTATGTGGTTGGGCAGGCGTTTAAGGCCGATAAGATTAAACTCACCGATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGAACAAAGGTGTGATTATCCAGTCCGGTAATGACGCCTGTATTGCGCTGGCTGATTACGTTGCCGGGAGCCAGGAGTCATTTATTGGTCTGATGAATg  >  1:225443/1‑251 (MQ=255)                                                                                                                                                                                                                      |                                                                                                                                                                                                                                        CGTGGTCTTGCAGCGGGTTCTGCATTTTTATTCCTTTTTGCCCCAACGGCATTCGCGGCGGAACAAACCGTTGAAGCGCCGAGCGTGGATGCGCGTGCATGGATTTTAATGGATTACGCCAGCGGTAAAGTGCTGGCAGAAGGCAATGCGGATGAGAAACTGGATCCCGCGAGCCTGACTAAAATCATGACCAGCTATGTGGTTGGGCAGGCGCTTAAGGCCGATAAGATTAAACTCACCGATATGGTGACGGTCGGTAAAGATGCCTGGGCGACGGGAAATCCGGCACTGCGTGGTTCATCGGTAATGTTCCTCAAACCGGGCGATCAGGTTTCGGTGGCAGACTTGAACAAAGGTGTGATTATCCAGTCCGGTAATGACGCCTGTATTGCGCTGGCTGATTACGTTGCCGGGAGCCAGGAGTCATTTATTGGTCTGATGAATG  >  NC_000913/880751‑881195

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑