Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	495,622	C→T	T168I (ACC→ATC)	htpG →	chaperone protein HtpG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	495,622	0	C	T	100.0%	127.6 / NA	40	T168I (ACC→ATC)	htpG	chaperone protein HtpG
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (16/24);  total (16/24)

CGCGACGAAGTGATTGACCATCTGGGGACTATCGCTAAATCCGGTACCAAATCATTCCTCGAATCCCTGGGTTCTGACCAGGCGAAAGACAGCCAGCTGATCGGTCAGTTTGGTGTTGGTTTCTACTCTGCGTTTATCGTGGCCGACAAAGTGACCGTGCGTACTCGCGCGGCAGGCGAAAAACCAGAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAGGTGAATACACCGTTGCCGACATCACCAAAGAAGATCGTGGTACTGAAATCACCCTGCATCTGCGTGAAGGCGAAGACGAGTTCCTCGATGACTGGCGCGTGCGTTCCATCATCAGCAAATACTCCGACCATATCGCGCTGCCGGTAGAGATCGAAAAACGCGAAGAGAAAGACGGCGAAACCGTTATCTCCTGGGAGAAAATCAACAAAGCGCAGGCGCTGTGGACTCGTAACAAGTCGGAAATCACCGATGAAGAG  >  NC_000913/495378‑495854                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                         cgcgACGAAGTGATTGACCATCTGGGGACTATCGCTAAATCCGGTACCAAATCATTCCTCGAATCCCTGGGTTCTGACCAGGCGAAAGACAGCCAGCTGATCGGTCAGTTTGGTGTTGGTTTCTACTCTGCGTTTAGCGGGGCCGACAAAGTGACCGTGCGTACTCGCGCGGCAGGCGAAAAACCAGAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAGTGGAATACATCGTTGCCGACATCATCAaaga                                                                                                                                                                                                                                    <  2:220539/251‑1 (MQ=255)      cgaAGTGATTGACCAGCTGGGGACTATCGCTAAATCCGGTACCGAATCATTCCTCGAATCCCTGGGTTCTGACCAGGCGAAAGACAGCCAGCTGATCGGTCAGTTTGGTGTGGGTTTCTACTCTGCGTTTATCGTGGCCGACAAAGTGTCCGTGCGTGCTCGCGCGGCAGGCGAAAAACCAGAGAATGGCGGCTTCGTGGAATCTGCTGGCGAAGTAGAATGCACCGTTGCCGACTTCATCGAGGAAGATc                                                                                                                                                                                                                               <  2:104453/251‑1 (MQ=255)         aGTGATTGACCATCTGGGGACTATCGCTAAATCCGGTACCAAATCATTCCTCGAATCCCTGGGTTCTGACCAGGCGAAAGACAGCCAGCTGATCGGTCAGTTTGGTGTTGGTTTCTACTCTGCGTTTATCGTGGCCGACAAAGTGACCGTGCGTACTCGCGCGGCAGGCGAAAAACCAGAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAGGTGAATACACCGTTGCCGACATCATCAAAGAAGATCGTg                                                                                                                                                                                                                            >  2:81774/1‑251 (MQ=255)                        ggggACTATCGCTAAATCCGGTACCAAATCATTCCTCGAATCCCTGGGTTCTGACCAGGCGAAAGACAGCCAGCTGATCGGTCAGTTTGGTGTTGGTTTCTACTCTGCGTTTATCGTGGCCGACAAAGTGACCGTGCGTACTCGCGCGGCAGGCGAAAAACCAGAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAGGTGAATACACCGTTGCCGACATCATCAAAGAAGATCGTGGTACTGAAATCAc                                                                                                                                                                                                               <  1:100822/249‑1 (MQ=255)                               aTCGCTAAATCCGGTACCAAATCATTCCTCGAATCCCTGGGTTCTGACCAGGCGAAAGACAGCCAGCTGATCGGTCAGTTTGGTGTTGGTTTCTACTCTGCGTTTATCGTGGCCGACAAAGTGACCGTGCGTACTCGCGCGGCAGGCGAAAAACCAGAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAGGTGAATACACCGTTGCCGACATCATCAAAGAAGATCGTGGTACTGAAATCACCCTGCATCt                                                                                                                                                                                                      >  1:78830/1‑251 (MQ=255)                                           ggTACCAAATCATTCCTCGAATCCCTGGGTTCTGACCAGGCGAAAGACAGCCAGCTGATCGGTCAGTTTGGTGTTGGTTTCTACTCTGCGTTTATCGTGGCCGACAAAGTGACCGTGCGTACTCGCGCGGCAGGCGAAAAACCAGAAAATGGCGTCTTCTGGGCATCGGCTGGCGAAGGTGAATACACCGTTGCCGACATCATCAAAGAAGATCGCGGTACTGAAATCACCCTGCATCTGCGTGAAGGCg                                                                                                                                                                                           >  1:263263/1‑250 (MQ=255)                                              atcAAATCATTCCTCGAATCCCTGGGTTCTGTCCAGGCGAAAGCCATCCAGCTGATCGGTCAGTTTGGTGTTGGTTTCCACTCTGCGTTTATCGTGGCCGACAAAGTTACCGTGCGTACTCGCGCTGCAGGCGAAAAACCAGAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAGGTGAATACACCGTTGCCGACATCATCAAAGAAGATCGTGGTACTGAAATCACCCTGCATCTTCGTGAAGGCGAAGa                                                                                                                                                                                       <  1:32049/249‑1 (MQ=255)                                                               aTCCCTGGGTTCTGACCAGGCGAAAGACAGCCAGCTGATCGGTCAGTTTGGTGTTGGTTTCTACTCTGCGTTTATCGTGGCCGACAAAGTGACCGTGCGTACTCGCGCGGCAGGCGAAAAACCAGAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAGGTGAATACACCGTTGCCGACATCATCAAAGAAGATCGTGGGACTGAAATCACCCTGCATCTGCGTGAAGGCGAAGACGAGTTCCTCGATGACt                                                                                                                                                                      >  2:5800/1‑251 (MQ=255)                                                               aTCCCTGGGTTCTGACCAGGCGAAAGACAGCCAGCTGATCGGTCAGTTTGGTGTTGGTTTCTACTCTGCGTTTATCGTGGCCGACAAAGTGACCGTGCGTACTCGCGCGGCAGGCGAAAAACCAGAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAGGGGAATACACCGTTGCCGACATCATCAAAGAAGATCGTGGTACTGAAATCACCCTGCATCTGCGTGAAGGCGAAGACGAGTTCCTCGATGACt                                                                                                                                                                      >  1:54307/1‑251 (MQ=255)                                                                         tCTGACCAGGCGAAAGACAGCCAGCTGATCGGTCAGTTTGGTGTTGGTTTCTACTCTGCGTTTATCGTGGCCGACAAAGTGACCGTGCGTACTCGCGCGGCAGGCGAAAAACCAGAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAGGTGAATACACCGTTGCCGACATCATCAAAGAAGATCGTGGTACTGAAATCACCCTGCATCTGCGTGAAGGCGAAGACGAGTTCCTCGATGACTGGCgcgtgc                                                                                                                                                             <  1:218125/250‑1 (MQ=255)                                                                            gACCAGGCGAAAGACAGCCAGCTGATCGGTCAGTTTGGTGTTGGTTTCTACTCTGCGTTTATCGTGGCCGACAAAGTGACCGTGCGTACTCGCGCGGCAGGCGAAAAACCAGAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAGGTGAATACACCGTTGCCGACATCATCAAAGAAGATCGTGGTACTGAAATCACCCTGCATCTGCGTGAAGGCGAAGACGAGTTCCTCGATGACTGGCGCGTGCGtt                                                                                                                                                          <  1:186298/250‑1 (MQ=255)                                                                                 ggCGAAAGACAGCCAGCTGATCGGTCAGTTTGGTGTTGGTTTCTACGCTGCGTTTATCGTGGCCGACAAAGTGACCGTGCGTACTCGCGCGGCAGTCGAAAAACCAGAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAGGTGAATACACCGTTGCCGACATCATCAAAGAAGATCGTGGTACTGAAATCACCCTGCATCGGCGTGAAGGCGAAGACGAGTTCCTCGATGACTGGCGCGTGCGTTCcatca                                                                                                                                                    <  2:36024/251‑1 (MQ=255)                                                                                   cGAAAGACAGCCAGCTGATCGGTCAGTTTGGTGTTGGTTTCTCCTCTGCGTTTCTCGTGGCCGACAAAGTGACCGTGCGTACTCGCGCGGCAGGCGAAAAACCAGAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAGGTGAATACACCGTTGCCGACATCATCAAAGAAGATAGTGGGACTGAAATAACCCTGAATCTGCGAGAAGGCGAAGACGAGTTCCTCGATGAcacgcccgggggctccatcagc                                                                                                                                                  >  2:42393/1‑230 (MQ=255)                                                                                    gAAAGACAGCCAGCTGATCGGTCAGTTTGGTGTTGGTTTCTACTCTGCGTTTATCGTGGCCGACAAAGTGACCGTGCGTACTCGCGCGGCAGGCGAAAAACCAGAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAGGTGAATACACCGTTGCCGACATCATCAAAGAAGATCGTGGTACTGAAATCACCCTGCATCTGCGTGAAGGCGAAGACGAGTTCCTCGATGACTGGCGCGTGCGTTCCatcatcc                                                                                                                                                 >  2:245181/1‑250 (MQ=255)                                                                                             ccagcTGATCGGTCAGTTTGGTGTTGGTTTCTACTCTGCGTTTATCGTGGCCGACAAAGTGACCGTGCGTACTCGCGCGGCAGGCGAAAAACCAGAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAGGTGAATACACCGTTGCCGACATCATCAAAGAAGATCGTGGTACTGAAATCACCCTGCATCTGCGTGAAGGCGAAGACGAGTTCCTCGATGACTGGCGCGTGCGTTCCATCATCAGCAAATACt                                                                                                                                        >  2:208902/1‑251 (MQ=255)                                                                                                        gtcagataggTGTTGGTTTCTACTCTGCGTTTATCGTGGCCGACCCAGTGACCGTGCGTACTCGCGCGACAGGCGCAAAACCAGAAAATGGCGTCTTCTGGAAATCGGCTGGCGAAGGTGAATACACCGTTGCCGACATCATCCAAGAAGATCGTGGTACTGAAATCACCCTGCATCTGCGTGACGGCGGAGACGTGATCCTCGATGCCTGGCGCTTGCGCTCCCTCATCCGCACATACTCCgcacctctc                                                                                                                             >  2:134240/9‑243 (MQ=255)                                                                                                        gTCAGTTTGGTGTTGGTTTCTACTCTGCGTTTATCGTGGCCGACAAAGTGACCGTGCGTACTCGCGCGGCAGGCGAAAAACCAGAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAGGTGAATACACCGTTGCCGACATCATCAAAGAAGATCGTGGTACTGAAATCACCCTGCATCTGCGTGAAGGCGAAGACGAGTTCCTCGATGACTGGCGCGTGCGTTCCATCATCAGCAAATACTCCGACCATATc                                                                                                                             <  1:218249/251‑1 (MQ=255)                                                                                                         gcAGTTTGGTGTTGGTTTCTACTCTGCGTTTATCGTGGCCGACAAAGTGACCGTGCGTACTCGCGCGGCAGGCGAAAAACCAGAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAGGTGAATACACCGTTGCCGACATCATCAAAGAAGATCGTGGTACTGAAATCACCCTGCATCTGCGTGAAGGCGAAGACGAGTTCCTCGATGACTGGCGCGTGCGTTCCATCATCAGCAAATACTCCGACCATATcg                                                                                                                            <  2:18383/250‑1 (MQ=255)                                                                                                                     tGGTTTCTACTCTGCTTTTAGCGTGTCCGTCAAAGTGACCGTGCGTACTCGCGCGGCAGGCGAAATACCAGAAAATGTCGTCTTCTGGGAATCGTCTGGCGAAGGTGAATACACCGTTGCCGACATCATCAAAGAAGATCGTGGTACTGAAATCACCCTGCAGCTGCGTGAAGGCGAAGACGAGTTCCTCGATGACGGGCGCGTGCGTTCCATCATCATCTAATACTCCTACCATATCGCGCCGCCGGTa                                                                                                                 <  2:163395/250‑1 (MQ=255)                                                                                                                             acgctGCGTGTATCGTGGCAGACAAAGGGTCCGTGCGTACGCGCGCGGCGGGGGAAAAACCAGAAAATGGCGTGTTCTGGGAATCGGCTGGCGAAGGTGAATACACCGTTGCCGACATTATCAAAGAAGATCGTGGTACTGTGATGACCCTGGATCGGCGTGAATGCGAAGACGAGGTCCTCGATGACTGGCGCGTGGGTGCCAGCATGAGCAAATACTCCGTCTATATCGCGCTGCCGGTAGAGATCGa                                                                                                         <  2:156662/247‑1 (MQ=255)                                                                                                                             aCTCTGCGTTTATCGTGGCCGACAAAGTGACCGTGCGTACTCGCGCGGCAGGCGAAAAACCAGAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAGGTGAATACACCGTTGCCGACATCATCAAAGAAGATCGTGGTACTGAAATCACCCTGCATCTGCGTGAAGGCGAAGACGAGTTCCTCGATGACTGGCGCGTGCGTTCCATCATCAGCAAATACGCCGACCATATCGCGCTGCCGGTAGAGATc                                                                                                           <  1:81774/248‑1 (MQ=255)                                                                                                                              ctctGCGTTTATCGTGGCCGACAAAGTGACCGTGCGTACTCGCGCGGCAGGCGAAAAACCAGAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAGGTGAATACACCGTTGCCGACATCATCAAAGCAGATCGTGGTACTGAAATCACCCTGCATCTGCGTGAAGGCGAAGACGAGTTCCTCGATGACTGGCGCGTGCGTTCCATCATCAGCAAATACTCCGACCATATCGCGCTGCCGGTAGAGATCGaa                                                                                                        >  1:257418/1‑250 (MQ=255)                                                                                                                                  gCGTTTATCGTGGCCGACAAAGTGACCGTGCGTACTCGCGCGGCAGGCGAAAAACCAGAAAATGGCGTCTGCGGGGAATCGGCTGGCGAAGGTGAATACACCGTTGCCGACATCATCAAAGAAGATCGTGGTACTGAAATCACCCTGCATCTGCGTGAAGGCGAAGACGAGTTCCTCGATGACTGGCGCGGGCGTACCATCATCAGCAAATACTCCGACCATATCGCGCTGCCGGTAGAGATCGaaaaa                                                                                                     <  2:231122/249‑1 (MQ=255)                                                                                                                                       tATCGTGGCCGACAAAGTGACCGTGCGTACTCGCGCGGCAGGCGAAAAACCAGAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAGGTGAATACACCGTTGCCGACATCATCAAAGAAGATCGTGGTACTGAAATCACCCTGCATCTGCGTGAAGGCGAAGACGAGTTCCTCGATGACTGGCGCGTGCGTTCCATCATCAGCAAATACTCCGACCATATCGCGCTGCCGGTAGAGATCGAAAAACGCGaa                                                                                               <  2:197550/250‑1 (MQ=255)                                                                                                                                        aacGTGGCCGACAAAGTGACCGTGCGTACGCGCGTGGCAGGCGAAAAACCAGAAAATGGCGTCTGCTGGGAAGCGGCTGGCTAAGGTGAATACACCGTTGCCGACATCATCAAAGAAGATCGTGGTACTGAAATCACCCTGCATCTGCGTGAAGGCGAAGACGAGTTCCTCGATGACTGGCGCGTGCGTTCCAGCATCAGCAAATACGCCGACCATATCGCGCTGCCGGTAGAGATCGAAAAACGCGAaga                                                                                             <  2:126501/249‑1 (MQ=255)                                                                                                                                                     aaGTGACCGTGCGTACTCGCGCGGCAGGCGAAAAACCAGAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAGGTGAATACACCGTTGCCGACATCATCAAAGAAGATCGTGGTACTGAAATCACCCTGCATCTGCGTGAAGGCGAAGACGAGTTCCTCGATGACTGGCGCGTGCGTTCCATCATCAGCAAATACTCCGACCATATCGCGCTGCCGGTAGAGATCGAAAAACGCGAAGAGAAAGACGGCGaa                                                                                <  2:181198/251‑1 (MQ=255)                                                                                                                                                         gACCGTGCGTACTCGCGCGGCAGGCGAAAAACCAGAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAGGTGAATACACCGTTGCCGACATCATCAAAGAAGATCGTGGTACTGAAATCACCCTGCATCTGCGTGAAGGCGAAGACGAGTTCCTCGATGACTGGCGCGTGCGTTCCATCATCAGCAAATACTCCGACCATATCGCGCTGCCGGTAGAGATCGAAAAACGCGAAGAGAAAGACGGCGAAACCg                                                                            <  1:5800/251‑1 (MQ=255)                                                                                                                                                                cGTACTCGCGCGGCAGGCGAAAAACCAGAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAGGTGAATACACCGTTGCCGACATCATCAAAGAAGATCGTGGTACTGAAATCACCCTGCATCTGCGTGAAGGCGAAGACGAGTTCCTCGATGACTGGCGCGTGCGTTCCATCATCAGCAAATACTCCGACCATATCGCGCTGCCGGTAGAGATCGAAAAACGCGAAGAGAAAGACGGCGAAACCGTTAtctc                                                                     >  1:63323/1‑251 (MQ=255)                                                                                                                                                                 gTACTCGGGCGGCAGGCGAAAAAACAGAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAGGGGAATACACCGTTGCCGACATCATCAAAGAAGATCGTGGTACTGAAATCACCCTGCATCTGCGTGAAGGCGAAGACGAGTTCCTCGATGACTGGCGCGTGCGTTCCATCATCAGCACATACTCCGACCATTTCGCGCTGCCGGTAGAGATCGAACAACGCGAAGAGAACGCCGGCGAAACCGTTAtctac                                                                    >  2:106278/1‑249 (MQ=255)                                                                                                                                                                 gTACTCGCGCGGCAGGCGAAAAACCAGAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAGGTGAATACACCGTTGCCGACATCATCAAAGAAGATCGTGGTACTGAAATCACCCTGCATCTGCGTGAAGGCGAAGACGAGTTCCTCGATGACTGGCGCGTGCGTTCCATCATCAGCAAATACTCCGACCATATCGCGCTGCCGGTAGAGATCGAAAAACGCGAAGAGAAAGACGGCGAAACCGTTATCTcc                                                                    <  1:245181/251‑1 (MQ=255)                                                                                                                                                                 gTACTCGCGCGGCAGGCGAAAAACCAGAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAGGTGAATACACCGTTGCCGACATCATCAAAGAAGATCGTGGTACTGAAATCACCCTGCATCTGCGTGAAGGCGAAGACGAGTTCCTCGATGACTGGCGCGTGCGTTCCATCATCAGCAAATACTCCGACCATATCGCGCTGCCGGTAGAGATCGAAAAACGCGAAGAGAAAGACGGCGAAACCGTTATCTcc                                                                    <  1:6481/251‑1 (MQ=255)                                                                                                                                                                   aCTCGCGCGGCAGGCGAAAAACCAGAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAGGTGAATACACCGTTGCCGACATCATCAAAGAAGATCGTGGTACTGAAATCACCCTGCATCTGCGTGAAGGCGAAGACGAGTTCCTCGATGACTGGCGCGTGCGTTCCATCATCAGCAAATACTCCGACCATATCGCGCTGCCGGTAGAGATCGAAAAACGCGAAGAGAAAGACGGCGAAACCGTTATCTCCTg                                                                  <  1:229007/251‑1 (MQ=255)                                                                                                                                                                       gcgcgGCAGGCGAAAAACCAGAAAATGTCGTCTTCTGGGAATCGGCTGGCGAAGGTGAATACACCGTGGCCGACAGCATCAAAGAAGATCGGGGTACTGAAATCACCCTGCTTCTGCGTGAAGGCGAAGACGAGTTCCTCGATGACGGGCGCGTGCGTTCCATCATCAGCAAATACTCCGACCATATCGCGCTGCCGGTAGAGAGCGAAAAACGCGAAGAGAAAGACGGCGAAACCGTTATCTCCTGGgag                                                              <  1:134240/251‑1 (MQ=255)                                                                                                                                                                              aGGCGAAAAACCAGAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAGGTGAATACACCGTTGCCGACATCATCAAAGAAGATCGTGGTACTGAAATCACCCTGCATCTGCGTGAAGGCGAAGACGAGTTCCTCGATGACTGGCGCGTGCGTTCCATCATCAGCAAATACTCCGACCATATCGCGCTGCCGGTAGAGATCGAAAAACGCGAAGAGAAAGACGGCGAAACCGTTATCTCCTGGGAGAAAATc                                                        >  1:71413/1‑250 (MQ=255)                                                                                                                                                                               ggCGAAAAACCAGAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAGGTGAATACACCGTTGCCGACATCATCAAAGAAGATCGTGGTACTGAAATCACCCTGCATCTGCGTGAAGGCGAAGACGAGTTCCTCGATGACTGGCGCGTGCGTTCCATCATCAGCAAATACTCCGACCATATCGCGCTGCCGGTAGAGATCGAAAAACGCGAAGAGAAAGACGGCGAAACCGTTATCTCCTGGGAGAAAATca                                                       >  2:98740/1‑250 (MQ=255)                                                                                                                                                                                                    gTCTTCTGGGAATCGGCTGGCGAAGGTGAATACACCGTTGCCGACATCATCAAAGAAGATCGTGGTACTGAAATCACCCTGCATCTGCGTGAAGGCGAAGACGAGTTCCTCGATGACTGGCGCGTGCGTTCCATCATCAGCAAATACTCCGACCATATCGCGCTGCCGGTAGAGATCGAAAAACGCGAAGAGAAAGACGGCGAAACCGTTATCTCCTGGGAGAAAATCAACAAAGCGCAGGCGCTGTGGAc                                 >  1:40399/1‑251 (MQ=255)                                                                                                                                                                                                           gggAATCGGCTGGCGAAGGTGAATACCCCGTTGCCGACATCATCAAAGAAGATCGTGGTACTGAAATCACCCTGCATCTGCGTGAAGGCGAAGACGAGTTCCTCGATGACTGGCGCGTGCGTTCCATCATCAGCAAATACTCCGACCATATCGCGCTGCCGGTAGAGATCGAAAAACGCGAAGAGAAAGACGGCGAAACCGTTATCTCCTGGGAGAAAATCAACAAAGCGCAGGCGCTGTGGACTCGTaa                           <  2:78830/250‑1 (MQ=255)                                                                                                                                                                                                           gggAATCGGCTGGCGAAGGTGAATACACCGTTGCCGACATCATCAAAGAAGATCGTGGTACTGAAATCACCCTGCATCTGCGTGAAGGCGAAGACGAGTTCCTCGATGACTGGCGCGTGCGTTCCATCATCAGCAAATACTCCGACCATATCGCGCTGCCGGTAGAGATCGAAAAACGCGAAGAGAAAGACGGCGAAACCGTTATCTCCTGGGAGAAAATCAACAAAGCGCAGGCGCTGTGGACTCGt                             <  1:208902/248‑1 (MQ=255)                                                                                                                                                                                                                        cGAAGGTGAATACACCGTTGCCGACATCATCAAAGAAGATCGTGGTACTGAAATCACCCTGCATCTGCGTGAAGGCGAAGACGAGTTCCTCGCTGACTGGCGCGTGCGTTCCATCATCAGCAAATACTCCGACCATATCGCGCTGCCGGTAGAGATCGAAAAACGCGAAGAGAAAGACGGCGAAACCGTTATCTCCTGGGAGAAAATCAACAAAGCGCAGGCGCTGTGGACTCGTAACAAGTCGGAAATc              >  2:243509/1‑250 (MQ=255)                                                                                                                                                                                                                                   acacCGTTGCCGACATCATCAAAGAAGATCGTGGTACTGAAATCACCCTGCATCTGCGTGAAGGCGAAGACGAGTTCCTCGATGACTGGCGCGTGCGTTCCATCATCAGCAAATACTCCGACCATATCGCGCTGCCGGTAGAGATCGAAAAACGCGAAGAGAAAGACGGCGAAACCGTTATCTCCTGGGAGAAAATCAACAAAGCGCAGGCGCTGTGGACTCGTAACAAGTCGGAAATCACCGATGAagag  <  1:134316/251‑1 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                         CGCGACGAAGTGATTGACCATCTGGGGACTATCGCTAAATCCGGTACCAAATCATTCCTCGAATCCCTGGGTTCTGACCAGGCGAAAGACAGCCAGCTGATCGGTCAGTTTGGTGTTGGTTTCTACTCTGCGTTTATCGTGGCCGACAAAGTGACCGTGCGTACTCGCGCGGCAGGCGAAAAACCAGAAAATGGCGTCTTCTGGGAATCGGCTGGCGAAGGTGAATACACCGTTGCCGACATCACCAAAGAAGATCGTGGTACTGAAATCACCCTGCATCTGCGTGAAGGCGAAGACGAGTTCCTCGATGACTGGCGCGTGCGTTCCATCATCAGCAAATACTCCGACCATATCGCGCTGCCGGTAGAGATCGAAAAACGCGAAGAGAAAGACGGCGAAACCGTTATCTCCTGGGAGAAAATCAACAAAGCGCAGGCGCTGTGGACTCGTAACAAGTCGGAAATCACCGATGAAGAG  >  NC_000913/495378‑495854

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑