Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	487,341	C→T	A269V (GCG→GTG)	mscK →	potassium dependent, small conductance mechanosensitive channel

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	487,341	0	C	T	97.1%	106.2 / NA	35	A269V (GCG→GTG)	mscK	potassium dependent, small conductance mechanosensitive channel
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base T (12/22);  total (13/22)
Fisher's exact test for biased strand distribution p-value = 3.71e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.73e-01

GGGACTGATGTCGGCGAGACAGCCTTACGTCCCAGCCAGAAAGTGTTAATGCAGGCCCAGCAGGCGTTGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAAGCGGTAAACAGCAAGCGCCTGA‑CTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAATTAACCAGCAGTTAAGTCAGCGTCTGATTACCGCGACTGAAAACGGTAATCAGTTGATGCAGCAAAACATTAAAGTCAAAAACTGGCTGGAGCGGGCGCTGCAATCGGAACGCAATATTAAAGAGCAGATTGCCGTCCTGAAGGGCAGCCTGCTGTTGTCTCGTATCCTT  >  NC_000913/487097‑487582                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                  gggACTGAGGTCGGCGAGACAGCCTTACGTCCCAGCCAGAAAGTGTTAATGCAGGCCCAGCAGGCGTTGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAAGCGGTAAACAGCAAGCGCCTGA‑CTTTAACCGAAAAAACGGTGCAgg                                                                                                                                                                                                                                              <  2:47660/250‑1 (MQ=255)       gATGTCGGCGAGACAGCCTTACGTCCCAGCCAGAAAGTGTTAATGCAGGGCCAGCAGGCGTTGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGAGTACGTGACGGCGAACAGCGCTCGTCTGGAGCGCCAGTTACAACTGTTGCAGGAAGCGGTAAGCAGCAAGCGCCTGG‑CATTAACCGAAAAAACGGTGCAGGAAGCCg                                                                                                                                                                                                                                        <  2:227514/250‑1 (MQ=255)          gTCGGCGAGACAGCCTTACGTCCCAGCCAGAAAGTGTTAATGCAGGCCCAGCAGGCGTTGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAAGCGGTAAACAGCAAGCGCCTGA‑CTTTAACCGAAAAAACGGTGCAGGAAGCCGtctc                                                                                                                                                                                                                                    <  2:69293/251‑1 (MQ=255)                          tACGTCCCAGCCAGAAAGTGTTAATGCAGGCCCAGCAGGCGTTGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAAGCGGTAAACAGCAAGCGCCTGA‑CTTTAACCGAAAAAACGGTGCAGGAAGCCGTCTCCCCGGATGAAGCcg                                                                                                                                                                                                                      <  2:70391/249‑1 (MQ=255)                             gTCCCAGCCAGAAAGTGATAATGCAGGCCAAGCAGGCGTTGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAAAGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAAGCGGTAAACAGCAAGCGCCTGA‑CTTTAACCGAAAAAACGGTGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGt                                                                                                                                                                                                                 >  1:184622/1‑251 (MQ=255)                              gcccAGCCAGAAAGTGTTAATGCAGGCCCAGCAGGCGTTGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCTCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAAGCGGTAAACAGCAAGCGCCTGA‑CTTTAACCGAAAAAACGGTGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTa                                                                                                                                                                                                                <  1:41399/250‑1 (MQ=255)                                       gAAAGTGTTAATGCAGGCCCAGCAGGCGTTGCTGAATGCGGAGCTTGCCCAGCAGCGTAAACGCATGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAAGCGGTAAACAGCAAGCGCCTGC‑ATTTAACCGAAAAAACGGTGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCCGGCt                                                                                                                                                                                                        >  2:259025/1‑250 (MQ=255)                                                         ccAGCAGGCGTTGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAAGCGGTAAACAGCAAGCGCCTGA‑CTTTAACCGAAAAA‑CGGTGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGa                                                                                                                                                                                    <  1:215979/251‑1 (MQ=255)                                                            gcagGCGTTGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACACCGTCTTGCCGGATACCTTGCAACAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAAGCGGTAAACAGCAAGCGCCTGA‑CTTTAACCGAAAAAACGGTGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTACTCCGCTGGTGAAGCAGGAAc                                                                                                                                                                                  >  2:108666/1‑251 (MQ=255)                                                             cagGCGTTGCTGAATGCGGAGAGTGACCAGCAGCGTAAAAGCCTGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAAGCGGTAAACAGCAAGCGCCTGA‑CTTTAACCGAAAAAACGGTGCAGGAAGCCGTCGCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCTCTGGTGAAGCATGAACt                                                                                                                                                                                 <  2:17143/251‑1 (MQ=255)                                                                 cGTTGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAAGCGGTAAACAGCAAGCGCCTGA‑CTTTAACCGAAAAAACGGTGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGaa                                                                                                                                                                             <  1:85253/251‑1 (MQ=255)                                                                                 gaTTGACCAGCAGCGTAAAAGCCTGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAAGCGGTAAACAGCAAGCGCCTGA‑CTTTAACCGAAAAAACGGTGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAATTAACCAGCAGTTa                                                                                                                                                              >  2:78912/1‑250 (MQ=255)                                                                                  aTTGACCAGCAGCGTAAAAGCCTGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAAGCGGTAAACAGCAAGCGCCTGA‑CTTTAACCGAAAAAACGGTGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAATTAACCAGCAGTTAAg                                                                                                                                                            <  2:73406/251‑1 (MQ=255)                                                                                     gACCAGCAGCGTAAAAGCCTGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAAGCGGTAAACAGCAAGCGCCTGA‑CTTTAACCGAAAAAACGGTGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAATTAACCAGCAGTTAAGTCa                                                                                                                                                         <  1:203916/251‑1 (MQ=255)                                                                                       ccAGCAGAGTAAAAGCCTGGAAGGAAACACCGTCTTGCATGCTGCCTTGCAAAAGCAACTTGAGTACGGGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAAGCGGTAAACAGCAAGCGCC‑GAGCTTTAACGGAAAAAACGGTGCAGGAAGTCGTGTCCCCGGAGGAAGCCGCGCTTATTCAGTCTAAGCCGCTGTTGAGGCAGGAACTGGGAGTGATCCGGCAGTTAAGTCAGc                                                                                                                                                       <  2:110582/251‑1 (MQ=255)                                                                                                   aaGCCTGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAAGCGGTAAACAGCAAGCGCCTGA‑CTTTAACCGAAAAAACGGTGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAATTAACCAGCAGTTAAGTCAGCGTCTGATTACcg                                                                                                                                           <  1:44789/251‑1 (MQ=255)                                                                                                         ggcaGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGCTTACGTGACGGCGAAAAGCCCTCGTCTCGAGCACCAGTTACAAATGTTGCAAGAAGCGGTAAACAGCAAGCGCCTGA‑CATTATCGGAAAAAACGGTGCAGGAAGCCGTGTCCCCGGATGAGGCCGCGCGTATTCAGGCTAATCCGCTTGTGAAGCAGGAACTGGAAATTAACCAGCAAGTAAGTCAGCGTCTGATTCCCGCGGCTg                                                                                                                                     >  1:22607/4‑251 (MQ=255)                                                                                                         ggAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAAGCGGTAAACAGCAAGCGCCTGA‑CTTTAACCGAAAAAACGGTGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAATTAACCAGCAGTTAAGTCAGCGTCTGATTACCGCGACTg                                                                                                                                     >  1:22597/1‑251 (MQ=255)                                                                                                               gAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGATGCAAGAAGCGGTAAACAGCAAGCGCCTGA‑CTTTAACCGAAAAAACGGTGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAATTAACCAGCAGTTAAGTCAGCGTCTGATCACCGCGACTGaaaa                                                                                                                                 >  1:246883/1‑249 (MQ=255)                                                                                                                aaCACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAAGCGGTAAACAGCAAGCGCCTGA‑CTTTAACCGAAAAAACGGTGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAATTAACCAGCAGTTAAGTCAGCGTCTGATTACCGCGACTGAAAACgg                                                                                                                              >  1:71864/1‑251 (MQ=255)                                                                                                                          tGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAAGCGGTAAACAGCAAGCGCCTGA‑CTTTAACCGAAAAAACGGTGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAATTAACCAGCAGTTAAGTCAGCGTCTGATTACCGCGACTGAAAACGGTAATCAGtt                                                                                                                     <  1:140171/250‑1 (MQ=255)                                                                                                                                aTACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAAGCGGTAAACAGCAAGCGCCTGA‑CTTTAACCGAAAAAACGGTGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAATTAACCAGCAGTTAAGTCAGCGTCTGATTACCGCGACTGAAAACGGTAATCAGTTGATgcag                                                                                                              <  2:194214/251‑1 (MQ=255)                                                                                                                                   ccTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAAGCGGTAAACAGCACGCGCCTGA‑CTTTAACCGAAAAAACGGTGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAATTAACCAGCAGTTAAGTCAGCGTCTGATTACCGCGACTGAAAACGGTAATCAGTTGATg                                                                                                                 >  1:172511/1‑245 (MQ=255)                                                                                                                                   ccTTGCAAAAGCAACGGGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAAGCGGTAAACAGCAAGCGCCTGA‑CTTTAACCGAAAAAACGGTGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAATTAACCAGCAGTTAAGTCAGCGTCTGATGACCGCGACTGAAAACGGTAATCAGTTGATg                                                                                                                 <  2:172511/245‑1 (MQ=255)                                                                                                                                              cAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAAGCGGTAAACAGCAAGCGCCTGA‑CTGTAACCGAAAAAACGGTGCAGGAAGCCGTCTCCCCGTATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAATTAACCAGCAGTTAAGTCAGCGTCTGATTACCGCGACTGAAAACGGTAATCAGTTGATGCAGCAAAACATTAAAGt                                                                                                <  2:215403/251‑1 (MQ=255)                                                                                                                                                                       gcgcTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAAGCGGTAAACAGCAAGCGCCTGA‑CTTTAACCGAAAAAACGGTGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAATTAACCAGCAGTTAAGTCAGCGTCTGATTACCGCGACTGAAAACGGTAATCAGTTGATGCAGCAAAACATTAAAGTCAAAAACTGGCTGGAGCGGGCGCTg                                                                       <  2:71864/251‑1 (MQ=255)                                                                                                                                                                             gtatGGAGCACCAGTTACAACTGTTGCAAGAAGCGGTAAACAGCAAGCGCCTGA‑CTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCACGGATGACGACGCGCGTATTCAGGCTAATCCGCTGGTGAAGCCGGAACTGGAAATTAACCAGCAGTTAAGTCAGCGTCTGATTACCGCGCCTGAAAACGGTCATCAGTTGATGCAGCAAATCATTACAGACAAACActgcctggggcacgcgccgcactcg                                                                 >  2:5688/4‑234 (MQ=255)                                                                                                                                                                                          gTTACAACTGTTGCAAGAAGCGGTAAACAGCAAGCGCCTGA‑CTTTAACCGAAAAAACGGTGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAATTAACCAGCAGTTAAGTCAGCGTCTGATTACCGCGACTGAAAACGGTAATCAGTGGATGCAGCAAAACATTAAAGTCAAAAACGGGCTGGAGCGGGCGCTGCAATCGGAACGCAATATTa                                                    <  1:250089/251‑1 (MQ=255)                                                                                                                                                                                           ttACAACTGTTGCAAGAAGCGGTAAACAGCAAGCGCCTGA‑CTTTAACCGAAAAAACGGTGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAATTAACCAGCAGTTAAGTCAGCGTCTGATTACCGCGACTGAAAACGGTAATCAGTTGATGCAGCAAAACATTAAAGTCAAAAACTGGCTGGAGCGGGCGCTGCAATCGGAACGCaaaataa                                                    >  2:183674/1‑245 (MQ=255)                                                                                                                                                                                              cAACTGTTGCAAGAAGCGGTAAACAGCAAGCGCCTGA‑CTTTAACCGAAAAAACGGTGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAATTAACCAGCAGTTAAGTCAGCGTCTGATTACCGCGACTGAAAACGGTAATCAGTTGATGCAGCAAAACATTAAAGTCAAAAACTGGCTGGAGCGGGCGCTGCAATCGGAACGCAATATTaa                                                   >  1:65835/1‑248 (MQ=255)                                                                                                                                                                                                                              ccTGA‑CTTTAACCGAAAAAACGGTGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAATTAACCAGCAGTTAAGTCAGCGTCTGATTACCGCGACTGAAAACGGTAATCAGTTGATGCAGCAAAACATTAAAGTCAAAAACTGGCTGGAGCGGGCGCTGCAATCGGAACGCAATATTAAAGAGCAGATTGCCGTCCTGAAGGGCAGCCTGCtgt                <  1:108666/251‑1 (MQ=255)                                                                                                                                                                                                                                 gA‑CTTTAACCGAAAAAACGTTGCCGCATGCCGTCTCGCCGGATGAAGCGGCGCGTATTCATGCTAATCCGCTGGGGAAGCAGGAACGGGAAATTAACCAGCAGTTAAGTCAGCGTCTGATTACCGCGACTGAAAACGGTAATCAGCTGATGCAGCAAAACATTAAAGTCAAAAACTGTCTGGAGCGGGCGCTGCAATCGGAACGCGATATGAAAGAGCAGATTCCCGTCCGGAAATTCAGCCTGCtgtt               <  2:22607/249‑1 (MQ=255)                                                                                                                                                                                                                                 gA‑CTTTAACCGAAAAAACGGTGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAATTAACCAGCAGTTAAGTCAGCGTCTGATTACCGCGACTGAAAACGGTAATCAGTTGATGCAGCAAAACATTAAAGTCAAAAACTGGCTGGAGCGGGCGCTGCAATCGGAACGCAATATTAAAGAGCAGATTGCCGTCCTGAAGTGCAGCCTGCtgtt               <  2:22597/249‑1 (MQ=255)                                                                                                                                                                                                                                          ccGAAAAAACGGTGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAATTAACCAGCAGTTAAGTCAGCGTCTGATTACCGCGACTGAAAACGGTAATCAGTTGATGCAGCAAAACATTAAAGTCAAAAACTGGCTGGAGCGGGCGCTGCAATCGGAACGCAATATTAAAGAGCAGATTGCCGTCCTGAAGGGCAGCCTGCTGTTGTCTCGTATc     >  1:115952/1‑251 (MQ=255)                                                                                                                                                                                                                                             aaaaaaCGGTGCAGGAAGCCGGCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAATTAACCAGCAGTTAAGTCAGCGTCTGATTACCGCGACTGAAAACGGTAATCAGTTGATGCAGCAAAACATTAAAGTCAAAAACTGGCTGGAGCGGGCGCTGCAATCGGAACGCAATAGTAAAGAGCAGATTGCAGTCCTGAAGTGCAGCCTGCTGTTGTCTCGTATCCtt  <  2:220625/251‑1 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                  GGGACTGATGTCGGCGAGACAGCCTTACGTCCCAGCCAGAAAGTGTTAATGCAGGCCCAGCAGGCGTTGCTGAATGCGGAGATTGACCAGCAGCGTAAAAGCCTGGAAGGGAACACCGTCTTGCAGGATACCTTGCAAAAGCAACGTGATTACGTGACGGCGAACAGCGCTCGTCTGGAGCACCAGTTACAACTGTTGCAAGAAGCGGTAAACAGCAAGCGCCTGA‑CTTTAACCGAAAAAACGGCGCAGGAAGCCGTCTCCCCGGATGAAGCCGCGCGTATTCAGGCTAATCCGCTGGTGAAGCAGGAACTGGAAATTAACCAGCAGTTAAGTCAGCGTCTGATTACCGCGACTGAAAACGGTAATCAGTTGATGCAGCAAAACATTAAAGTCAAAAACTGGCTGGAGCGGGCGCTGCAATCGGAACGCAATATTAAAGAGCAGATTGCCGTCCTGAAGGGCAGCCTGCTGTTGTCTCGTATCCTT  >  NC_000913/487097‑487582

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑