Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 478,372 C→T M82I (ATG→ATA)  pdeB ← c‑di‑GMP phosphodiesterase PdeB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913478,3720CT96.2% 78.3 / NA 26M82I (ATG→ATA) pdeBc‑di‑GMP phosphodiesterase PdeB
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (13/12);  minor base G (1/0);  total (14/12)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACATCAATAAAGGAAGCGGGGTCGATCATGACGACATAATGTGCCGTTCCCATGGCGACCATGTAACGGATAATGCCTAAATCGTTATGCGATGTTAACCAGACACGATAACCATCTTTCGAAATTTTACCTGGCTCGGGGAAGGTATCGGGCGGACTTTCATGCTCCAGAGACGAACACTGGGGAACGTTGTTATCGATATAAGCCACTTCCTGAATATAGCGATAACTGTAAGATACCCGACGCATTTCCATGAGATGGGCTTCGCTACAGGCAGCGCCTTGCCATCTTTCCAGCTCCTGCAGCGCATCTTTCCCTTGTGTCGCCACCTTATTGGCTCGAATAGCGACGCGGGAGGAATAGGTATCCAGCTCTTCAATAAACGATGTTTCTACCTGCTGATGGGCCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCT  >  NC_000913/478127‑478614
                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                  
aCATCAATAAAGGAAGCGGGGTCGATCATGACGACATAATGTGCCGTTCCCATGGCGACCATGTAACGGATAATGCCTAAATCGTTATGCGATGTTAACCAGACACGATAACCATCTTTCGAAATTTTACCTGGCTCGGGGAAGGTATCGGGCGGACTTTCATGCTCCAGAGACGAACACTGGGGAACGTTGTTATCGATATAAGCCACTTCCTGAATATAGCGATAACTGTAAGATACCCGACGTATTTc                                                                                                                                                                                                                                               <  2:227305/251‑1 (MQ=255)
aCATCAATAAAGGAAGCGGGGTCGATCATGACGACATAATGTGCCGTTCCCATGGCGACCATGTAACGGATAATGCCTAAATCGTTATGCGATGTTAACCAGACACGATAACCATCTTTCGAAATTTGACCTGGCTCGGGGAAGGTATCGGGCGGACTTTCATGCTCCAGAGACGAACACTGGGGAACGTTGTTATCGATATAAGCCACTTCCTGAATATAGCGATAACTGTAAGATACCCGACGTATTTc                                                                                                                                                                                                                                               <  1:103455/251‑1 (MQ=255)
                           aTGACGACATAATGTGCCGTTCCCATGGCGACCATGTAAAGGATAATGCCTAAATCGTTATGCGCTGTTAACCAGACACGATAACCCTCTTTAGAAATTTTACCTGACTCGGTGAAGGTATCGGGCGGACTTTCATGCTCCAGAGACGAACACTGGGGAACGTTGTTATCGATATAAGCCACTTCCTGAATCTAGCGATAACTGTAAGATACCCGACGTATTTCCATGAGATGGGCTTCGCTACAGGccgc                                                                                                                                                                                                                    >  2:30925/1‑248 (MQ=255)
                                       tgagCCGTTCCCATGGAGACCAGGTAACGGATAATGCCTAAATAGTTATGCGATGTTAACCAGACACGATAACCATCTTTCGAAATTTTACCTGGCTCGGGGAAGGTATCGGGCGGACTTTCATGCTCCAGCGACGAACACTGAGGAACGTTGTTATCGATATAAGCCACTTCCTGACTATAGCGGTAACTGTAAGATACCCGACGGATTTCCATGAGATGGGCTTCGCTACAGCCAGCGCCTTGCCATCt                                                                                                                                                                                                        >  2:128984/4‑251 (MQ=255)
                                          gCCGTTCCCAAGGCGACCATGTAACGGATAATGCCTAAATCGTTATGCGATGTTAACCAGACACGATAACCATCTTTCGAAATTTTACCTGGCTCGGGGAAGGTATCGGGCGGACTTTCATGCTCCAGAGACGAACACTGGGGAACGTTGTTATCGATATAAGCCACTTCCTGAATATAGCGATAACTGTAAGATACCCGACGTATTTCCATGAGATGGGCTTCGCTACAGGCAGCGCCTTGCCATCttt                                                                                                                                                                                                      <  1:183709/250‑1 (MQ=255)
                                                            aTGTAACGGATAATGCCTAAATCGTTATGCGATGTTAACCAGACACGATAACCATCTTTCGAAATTTTACCTGGCTCGGGGCAGGTATCGGGCGGACTTTCATGCTCCAGAGACGAACACTGGGGAACGTTGTTATCGATATAAGCCACTTCCTGAATATAGCGATAACTGTAAGATACCCGACGTATTTCCATGAGATGGGCTTCGCTACAGGCAGCGCCTTGCCATCTTTCCAGCTCCTGCCGCGCATc                                                                                                                                                                                   >  1:121030/1‑251 (MQ=255)
                                                                  cGGATAATGCCTAAATCGTTATGCGATGTTAACCAGACACGATAACCATCTTTCGAAATTTTACCTGGCTCGGGGAAGGTATCGGGCGGACTTTCATGCTCCAGAGACGAACACTGGGGAACGTTGTTATCGATATAAGCCACTTCCTGAATATAGCGATAACTGTAAGATACCCGACGTATTTCCATGAGATGGGCTTCGCTACAGGCAGCGCCTTGCCATCTTTCCAGCTCCTGCAGCGCATCTTTccc                                                                                                                                                                             >  1:5575/1‑251 (MQ=255)
                                                                                    ttATGCGATGTTAACCAGACACGATAACCATCTTTCGAAATTTTCCCTGGCTCGGGGAAGGTATCGGGCGGACTTTCATGCTCCAGAGACGAACAATGGGGAACGTTGGTATCGATATAAGCCACTTCCTGAATATAGAGATACCTGTAAGATACCCGACGTATTTCCATGAGATGGGCTTCGCTACAGGCAGCGCCTTGCCATCTTTCCAGCTCCTGCAGCGCATCTTTCCCTTGTGTCGCCACCTTAtt                                                                                                                                                           >  2:225263/1‑251 (MQ=255)
                                                                                             gTTAACCAGACACGATAACCATCTTTCGAAATTTTACCTGGCTCGGGGAAGGTATCGGGCGGACTTTCATGCTCCAGAGACGAACACTGGGGAACGTTGTTATCGATATAAGCCACTTCCTGAATATAGCGATAACTGTAAGATACCCGACGTATTTCCATGAGATGGGCTTCGCTACAGGCAGCGCCTTGCCATCTTTCCAGCTCCTGCAGCGCATCTTTCCCTTGTGTCGCCACCTTATTGGCTCGAAt                                                                                                                                                  <  1:119099/251‑1 (MQ=255)
                                                                                                 aCCAGACACGATAACCATCTTTCGAAATTTTACCTGGCTCGGGGAAGGTATCGGGCGGACTTTCATGCTCCAGAGACGAACACTGGGGAACGTTGTTATCGATATAAGCCACTTCCTGAATATAGCGATAACTGTAAGATACCCGACGTATTTCCATGAGATGGGCTTCGCTACAGGCAGCGCCTTGCCATCTTTCCAGCTCCTGCAGCGCATCTTTCCCTTGTGTCGCCACCTTATTGGCTCGAATAGCg                                                                                                                                              <  2:192957/251‑1 (MQ=255)
                                                                                                                 aTCTTTCGAAATTTTACCTGGCTCGGGGCAGGTATCGGGCGGACTTTCATGATCCAGAGCCGAACAATGGGGAACATTGTTATCGATATAAGCCACTTCCTGAATATAGCGATAACTGTAAGCTACCCGACGTATTTCCATGAGATGAGCTTCGCTACAGGCAGCGCCTTGCCATCTTTCCAGCTCCTGCAGCGCATCTTTCCCTTGTGTAGCCACCTTATTGGCTCGACTAGCGACGCGGGAGGACTAgg                                                                                                                              >  1:32548/1‑251 (MQ=255)
                                                                                                                    tttCGAAATTTTACCTGGCTCGGGGAAGGTATCGGGCGGACTTTCATGCTCCAGAGACGAACACTGGGGAACGTTGTTATCGATATAAGCCACTTCCTGAATATAGCGATAACTGTAAGATACCCGACGTATTTCCATGAGATGGGCTTCGCTACAGGCAGCGCCTTGCCATCTTTCCAGCTCCTGCAGCGCATCTTTCCCTTGTGTCGCCACCTTATTGGCTCGAATAGCGACGCGGGAGGAATAGGTAt                                                                                                                           >  1:89341/1‑251 (MQ=255)
                                                                                                                    tttCGAAATTTTACCTGGCTCGGGGAAGGTATCGGGCGGACTTTCATGCTCCAGAGACGAACACTGGGGAACGTTGTTATCGATATAAGCCACTTCCTGAATATAGCGATAACTGTAAGATACCCGACGTATTTCCATGAGATGGGCTTCGCTACAGGCAGCGCCTTGCCATCTTTCCAGCTACTGCAGCGCATCTTTCCCTTGTGTCGCCCCCTTATTGGCTCGGATAGCGACCCGGGAGGAATAgggat                                                                                                                           >  2:44147/1‑248 (MQ=255)
                                                                                                                                       gatcgtccgatcgcGGGCGGACGTTCATGCTCCAGAGACGAAAACTGGGGAAAGTGGTTATCGATATAAGCCACTGCCTGAATATAGCGATAACTGTAAGATACCCGACGTATTTCCATGAGATGGGCTTCGCTACAGGCAGCGCCTTGCCATCTTTCCAGCTCCTGCAGCGCATCGTGCCCTTGTGGCGCCACCTTATTGGCTCGAATAGCGACGCGGGAGGAATAGGTATCCAGCTCTTCAATAAACGa                                                                                                        <  2:93487/238‑1 (MQ=255)
                                                                                                                                                    cGGGCGGACTTTCATGCTCCAGAGACGAACACTGGGGAACGTTGTTATCGATATAAGCCACTTCCTGCATATAGCGATAACTGTAAGATACCCGACGTATTTCCATGAGATGGGCTTCGCTACAGGCAGCGCCTTGCCATCTTTCCAGCTCCTGCAGCGCATCTTTCCCTTGTGTCGCCACCTTATTGGCTCGAATAGCGACGCGGGAGGAATAGGTATCCAGCTCTTCCATAAACGa                                                                                                        >  1:93487/1‑238 (MQ=255)
                                                                                                                                                             tttCATGCTCCAGAGACGAACACTGGGGAACGTTGTTATCGATATAAGCCACTTCCTGAATATAGCGCTAACTGTAAGATACCCGACGTATTTCCATGAGATGGGCTTCGCTACAGGCAGCGCCTTGCCATCTTTACAGCTCCTGCAGCGCATTTTACCCTTGTGTCGCCACCTTATAGGCTCGACTAGCGCCGCGGGAGGAATAGGGATCCAGCTCTTCAATAAACGATTTTTCTACCTGCTGATGGgcc                                                                                  >  2:149891/1‑251 (MQ=255)
                                                                                                                                                              ttCATGCTCCAGAGACGAACACTGGGGAACGTTGTTATCGATATAAGCCACTTCCTGAATATAGCGATAACTGTAAGATACCCGACGTATTTCCATGAGATGGGCTTCGCTACAGGCAGCGCCTTGCCATCTTTCCAGCTCCTGCAGCGCATCTTTCCCTTGTGTCGCCACCGTATTGGCTCGAATAGCGACGCGGGAGGAATAGGTATCCAGCTCTTCAATAAACGATGTTTCTACCTGCTGATGGgcc                                                                                  <  1:110577/250‑1 (MQ=255)
                                                                                                                                                                                     ggggAACGTTGTTATCGATATAAGCCACTTCCTGAATATAGCGATAACTGTAAGATACCCGACGTATTTCCATGAGATGGGCTTCGCTACAGGCAGCGCCTTGCCATCTTTCCAGCTCCTGCAGCGCATCTTTCCCTTGTGTCGCCACCTTATTGGCTCGAATAGCGACGCGGGAGGAATAGGTATCCAGCTCTTCAATAAACGATGTTTCTACCTGCTGATGGGCCAGCCAGATGCTTAAGCCGACAg                                                            <  1:202634/249‑1 (MQ=255)
                                                                                                                                                                                        gAACGTTGTTATCGATATAAGCCACTTCCTGAATATAGCGATAACTGTAAGATACCCGACGTATTTCCATGAGATGGGCTTCGCTACAGGCAGCGCCTTGCCATCTTTCCAGCTCCTGCAGCGCATCTTTCCCTTGTGTCg                                                                                                                                                                     >  1:148450/1‑141 (MQ=255)
                                                                                                                                                                                        gAACGTTGTTATCGATATAAGCCACTGCCTGAATATAGCGATAACTGTAAGATACCCGACGTATTTCCATGAGATGGGCTTCGCTACAGGCAGCGCTTTGCCATCTTTCCAGCTCCTGCAGCGCATCTTTCCCATGTGTCg                                                                                                                                                                     <  2:148450/141‑1 (MQ=255)
                                                                                                                                                                                                                                      gTAAGATACCCGACGTATTTCCATGAGATGGGCTTCGCTACAGGCAGCGCCTTGCCATCTTTCCAGCTCCTGCAGCGCATCTTTCCCTTGTGTCGCCACCTTATTGGCTCGAATAGCGACGCGGGAGGAATAGGTATCCAGCTCTTCAATAAACGATGTTTCTACCTGCTGATGGGCCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGt          <  2:10032/250‑1 (MQ=255)
                                                                                                                                                                                                                                      gTAAGATACCCGACGTATTTCCATGAGATGGGCTTCGCTACAGGCAGCGCCTTGCCATCTTTCCAGCTCCTGCAGCGCATCTTTCCCTTGTGTCGCCACCTTATTGGCTCGAATAGCGACGCGGGAGGAATAGGTATCCAGCTCTTCAATAAACGATGTTTCTACCTGCTGATGGGCCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTc         <  1:30925/251‑1 (MQ=255)
                                                                                                                                                                                                                                      gTAAGATACCCGACGTATTTCCATGAGATGGGCTTCGCTACAGGCAGCGCCTTGCCATCTTTCCAGCTCCTGCAGCGCATCTTTCCCTTGTGTCGCCACCTTATTGGCTCGAATAGCGACGCGGAAGGAATAGGTATCCAGCTCTTCAATAAACGATGTTTCTACCTGCTGATGGGCCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTc         >  1:6624/1‑251 (MQ=255)
                                                                                                                                                                                                                                             aCCCGACGTATTTCCATGAGATGGGCTTCGCTACAGGCAGCGCCTTGCCATCTTTCCAGCTCCTGCAGCGCATCTTTCCCTTGTGTCGCCACCTTATTGGCTCGCATAGCGACGCGGGAGGAATAGGTATCCAGCTCTTCCATAAACGATGTTTCTACCTGCTGATGGGCCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGGAAGAATCAGTACTCCCGAAATACGGCCGGCCCGATGGCGTGGtct  >  2:66754/1‑251 (MQ=255)
                                                                                                                                                                                                                                             aCCCGACGTATTTCCATGAGATGGGCTTCGCTACAGGCAGCGCCTTGCCATCTTTCCAGCTCCTGCAGCGCATCTTTCCCTTGTGTCGCCACCTTATTGGCTCGAATAGCGACGCGGGAGGAATAGGTATCCAGCTCTTCAATAAACGATGTTTCTACCTGCTGATGGGCCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGTCCAGATGTCGTGtt    <  2:192978/249‑1 (MQ=255)
                                                                                                                                                                                                                                             aCCCGACGTATTTCCATGAGATGGGCTTCGCTACAGGCAGCGCCTTGCCATCTTTCCAGCTCCTGCAGCGCATCTTTCCCTTGTGTCGCCACCTTATTGGCTCGAATAGCGACGCGGGAGGAATAGGTATCCAGCTCTTCAATAAACGATGTTTCTACCTGCTGATGGGCCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGtt    >  1:192978/1‑249 (MQ=255)
                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                  
ACATCAATAAAGGAAGCGGGGTCGATCATGACGACATAATGTGCCGTTCCCATGGCGACCATGTAACGGATAATGCCTAAATCGTTATGCGATGTTAACCAGACACGATAACCATCTTTCGAAATTTTACCTGGCTCGGGGAAGGTATCGGGCGGACTTTCATGCTCCAGAGACGAACACTGGGGAACGTTGTTATCGATATAAGCCACTTCCTGAATATAGCGATAACTGTAAGATACCCGACGCATTTCCATGAGATGGGCTTCGCTACAGGCAGCGCCTTGCCATCTTTCCAGCTCCTGCAGCGCATCTTTCCCTTGTGTCGCCACCTTATTGGCTCGAATAGCGACGCGGGAGGAATAGGTATCCAGCTCTTCAATAAACGATGTTTCTACCTGCTGATGGGCCAGCCAGATGCTTAAGCCGACAGGCAGCAATACTGAAAGAATCAGTACTCCCGAAATAAGGCCGACCAGATGTCGTGTTCT  >  NC_000913/478127‑478614

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑