Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	473,963	C→T	P333L (CCC→CTC)	amtB →	ammonium transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	473,963	0	C	T	100.0%	63.4 / NA	25	P333L (CCC→CTC)	amtB	ammonium transporter
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (11/14);  total (11/14)

ATTTGTGAATACTGTGGTCGCAACGGCGGCGGCAATTCTTGGCTGGATCTTCGGTGAATGGGCGCTGCGTGGTAAGCCTTCACTGCTGGGGGCGTGTTCTGGCGCGATTGCCGGTCTGGTCGGCGTGACGCCAGCCTGCGGCTACATTGGGGTTGGCGGCGCGTTGATTATCGGCGTGGTAGCTGGTCTGGCGGGCTTGTGGGGCGTTACCATGCTCAAACGCTTGCTGCGGGTGGATGATCCCTGCGATGTCTTCGGTGTGCACGGCGT‑TTGTGGCATTGTCGGCTGTATCATG‑ACCGGGATTTTTGCCGCCAGCTCGCTGGGCGGCGTGGGCTTCGCTGAAGGTGTGACGATGGGCCATCAGTTGCTGGTACAGCTGGAAAGCATCGCCATTACGATCGTCTGGTCCGGTGTTGTGGCATTTATCGGCTACAAATTGGCGGATCTGACGGTTGGTCTGCGTGTACCGGAAGAGCAGGAGCGAG  >  NC_000913/473721‑474205                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                     agttGTGAATACTGGGGTCGCAAGGGCGGCGGCAATTCTTGGCTGGATCGTCGGTGAATGGGCGCTGCTTGGTAAGCCTTCGCTGCTGGGGGCGTGTTCTGGCGCGATTGCCGGGCTGGGCGGCGTGACGCCAGCCTGCGGCTACATTGGGGTTGGCGGCGCGTGGATTAGCGGCGTGGTAGCTGGTCGGGCGGGCTTGTGGGGCGTTACCATGCTCAAACGCTTGCTGCGGGGGGATGATCTCTTCGATg                                                                                                                                                                                                                                              <  2:125789/249‑1 (MQ=255)       gAATACTGTGGTCGCAACGGCGGCGGCAATTCTTGGCTGGATCTTCGGTGAATGGGCGCTGCGTGGTAAGCCTTCACTGCTGGGGGCGTGTTCTGGCGCGATTGCCGGTCTGGTCGGCGTGACGCCAGCCTGCGGCTACATTGGGGTTGGCGGCGCGTTGATTATCGGCGTGGTAGCTGGTCTGGCGGGCTTGTGGGGCGTTACCATGCTCAAACGCTTGCTGCGGGTGGATGATCTCTGCGATGTCTTc                                                                                                                                                                                                                                         <  1:243196/250‑1 (MQ=255)         aTACTGTGGCCGCAACGGCGGCGGCAAGTCTTGGCTGGATCTTCGGGGAATGGGCGCTGCGTGGTAAGCCTTCTCTGCTGGGGGCGTGTTCTGGCGCGATTGCCGGTCTGGTCGGCGTGACGCCAGCCTGCGGCTACATTGGGGTTGGCGGCGCGTTGATTATCGGCGTGGTAGCTGGTCTGGCGGGCTTGTGGGGCGTTACCATGCTCAAACGCTTGCTGCGGGTGGATGATCTCTGCGATGTCTTCGgt                                                                                                                                                                                                                                      <  2:67340/251‑1 (MQ=255)              gtgGTCGCAGTGGCTGCGGCAATCCTTGGCTGTATTTTCGGTGAATGGGCGCTGCGTGGTAAGCCTTCTCTGTGGGGGGCGTGTTCTGGCGCGATTGCCGGTCTGGTCGGCGTGAAGCCAGCCTGCGGCTACATTGGGGTTGGCGGCGCGTTGATTATCGGCGTGGTAGCTGGTCTGGCGGGCTTGTGGGGCGTTACCATGCTCAAACGCTTGCTGCGGGTGTATGATCTCTGCGATGTCTTCGGTGTGCa                                                                                                                                                                                                                                 <  2:102736/251‑1 (MQ=255)                    gCAACGGCGGCGGCAATTCTTGGCTGGATCTTCGGTGAATGGGCGCTGCGTGGTAAGCCTTCACTGCTGGGGGCGTGTTCTGGCGCGATTGCCGGTCTGGTCGGCGTGACGCCAGCCTGCGGCTACATTGGGGTTGGCGGCGCGTTGATTATCGGCGTGGTAGCTGGTCTGGCGGGCGTGGGGGGGGGTACCCTGCTCAAACGCCTGCTGCGGGTGGGTGGTTTTTGCGATGTCTTCGGGGTGCACGGCGt                                                                                                                                                                                                                           >  1:56701/1‑251 (MQ=255)                                 cAATTCTTGGCTGGATCTTCGGTGAATGGGCGCTGCGTGGTAAGCCTTCACTGCTGGGGGCGTGTTCTGGCGCGATTGCCGGTCTGGTCGGCGTGACGCCAGCCTGCGGCTACATTGGGGTTGGCGGCGCGTTGATTATCGGCGTGGTAGCTGGTCTGGCGGGCTTGTGGGGCGTTACCATGCTCAAACGCTTGCTGCGGGTGGATGATCTCTGCGATGTCTTCGGTGTGCACGGCGT‑TTGTGGCATTGTc                                                                                                                                                                                                             <  1:57543/251‑1 (MQ=255)                                        tGGCTGGATCTTCGGTGAATGGGCGCTGCGTGGTAAGCCTTCACTGCTGGGGGCGTGTTCTGGCGCGATTGCCGGTCTGGTCGGCGTGACGCCAGCCTGCGGCTACATTGGGGTTGGCGGCGCGTTGATTATCGGCGTGGTAGCTGGTCTGGCGGGCTTGTGGGGCGTTACCATGCTCAAACGCTTGCTGAGGGGGGGTGGTCTTCGCGGTGTCTTCGGTGGGCCCGGCGT‑TTTTGGGATTGTCGGcggtt                                                                                                                                                                                                      >  2:241299/1‑247 (MQ=255)                                                     ggTGAATGGGCGCTGGGGGGTAAGCCTTCACGGCTGGGGGCGTGTTCTGGCGCGATTGCCGGTCTGGTCGGCGTGACGCCAGCCTGCGGCTACATTGGGGTTGGCGGCGCGTTGATTATCGGCGTGGTAGCTGGTCTGGCGGGCTTGTGGGGCGTTACCATGCTCAAACGCTTGCTGCGGGTGGATGATCTCTGCGATGTCTTCGGTGTGCACGGCGT‑TTGTGGCATTGTCGGCTGTATCATG‑ACCGGGAt                                                                                                                                                                                        <  1:24700/251‑1 (MQ=255)                                                                        gTAAGCCTTCACTGCTGGGGGCGTGTTCTGGCGCGATTGCCGGTCTGGTCGGCGTGACGCCAGCCTGCGGCTACATTGGGGTTGGCGGCGCGTTGATTATCGGCGTGGTAGCTGGTCTGGCGGGCTTGTGGGGCGTTACCATGCTCAAACGCTTGCTGCGGGTGGATGATCTCTGCGATGTCTTCGGTGTGCACGGCGT‑TTGTGGCATTGTCGGCTGTATCATGGACCGG‑ATTTTTGCCGCCCGCTCGCTg                                                                                                                                                                     >  1:161196/1‑251 (MQ=255)                                                                                       tgGGGGCGTGTTCTGGCGCGATTGCCGGTCTGGTCGGCGTGACGCCAGCCTGCGGCTACATTGGGGTTGGCGGCGCGTTGATTATCGGCGTGGTAGCTGGTCTGGCGGGCTTGTGGGGCGTTACCATGCTCAAACGCTTGCTGCGGGGGGATGATCTCTGCGGTGTCTTCGGTGTGCACGGCGG‑TTGTGGCATTGGCGGCTGTATCATG‑ACCGGGATTTTTGCCGCCCGACCGCTGGGCGGCGTGGGCTTc                                                                                                                                                      >  1:92993/1‑251 (MQ=255)                                                                                           ggCGTGTTCTGGCGCGATTGCCGGTCTGGTCGGCGTGACGCCAGCCTGCGGCTACATTGGGGTTGGCGGCGCGTTGATTATCGGCGTGGTAGCTGGTCTGGCGGGCTTGTGGGGCGTTACCATGCTCAAACGCTTGCTGCGGGTGGATGATCTCTGCGATGTCTTCGGTGTGCACGGCGT‑TTGTGGCATTGTCGGCTGTATCATG‑ACCGGGATTTTTGCCGTCAGCTCGCTGGGCGGCGTGGGCTTCGCTg                                                                                                                                                  <  1:211393/251‑1 (MQ=255)                                                                                                                cGGTCTGGTCGGCGTGACGCCAGCCTGCGGCTACATTGGGGTTGGCGGCGCGTTGATTATCGGCGTGGTAGCTGGTCTGGCGGGCTTGTGGGGCGTTACCATGCTCAAACGCTTGCTGCGGGTGGATGATCTCTGCGATGTCTTCGGTGTGCACGGCGT‑TTGTGGCATTTTCGGCTGTATCATT‑ACCGGGATTTTTGCCGCCAGCTCGCTGGGCGGCGTGGGCTTCGCTGAAGGTGTGACGATGGGCCATc                                                                                                                             >  1:47386/1‑251 (MQ=255)                                                                                                                                  gCCAGCCTGCGGCTACATTGGGGTTGGCGGCGCGTTGATTATCGGCGTGGTAGCTGGTCTGGCGGGCTTGTGGGGCGTTACCATGCTCAAACGCTTGCTGCGGGTGGATGATCTCTGCGATGTCTTCGGTGTGCACGGCGT‑TTGTGGCATTGTCGGCTGTATCATG‑ACCGGGATTTTTGCCGCCAGCTCGCTGGGCGGCGTGGGCTTCGCTGAAGGTGTGACGATGGGCCATCAGTTGCTGGTACAGCTgg                                                                                                           <  1:82447/251‑1 (MQ=255)                                                                                                                                  gCCAGCCTGAGGCTACATTGGGGTTGGCGGCGCGTTGATTATCGGCGTGGTAGCTGGTCTGGCGGGCTTGTGGGGCGTTACCATGCTCAAACGCTTGCTGCGGGGGGATGATCTCTGCGATGTCTTCGGTGTGCACGGCGT‑TTGTGGCATTGTCGGCCGTATCATG‑ACCGGGATTTTTTCCGCCAGCTCGCTGGGCGGCGTGGGCTTCGCTGAAGGTGTGACGATGGGCCATCAGTTGCTGGGACAGCTgg                                                                                                           >  1:49647/1‑251 (MQ=255)                                                                                                                                           cGGCTACATTGGGGTTGGCGGCGCGTTGATTATCGGCGTGGTAGCTGGTCTGGCGGGCTTGTGGGGCGTTACCATGCTCAAACGCTTGCTGCGGGTGGATGATCTCTGCGATGTCTTCGGTGTGCACGGCGT‑TTGTGGCATTGTCGGCTGTATCATG‑ACCTGGATTTTTGCCGCCAGCTCGCTGGGCGGCGTGGGCTTCGCTGAAGGTGTGACGATGGGCCATCAGTTGCTGGTACAGCTGGAAAGCATCg                                                                                                  <  1:241299/251‑1 (MQ=255)                                                                                                                                             gCTATATTGGGTTTGGCGGCGCGTTGATGAGCGGCGGGGTAGCTGGTGTTGCGTGCTTGTGTGGCGTTACCATGCTCAAACGCTTGCTGGGGGTGGATGATCTCTGCGATGTCTTCGGTGTGCACGGCGT‑TTGTGGCATTGTCGGCTGTATCATG‑ACCGGGATTTTTGCCGCCAGCTCGCTGGGCGGCGTGGTCTTCGCTGAAGTTGTGACGATGGGCCATCAGTTGCTGGTACATCTGGATAGCTTCGcc                                                                                                <  2:113923/251‑1 (MQ=255)                                                                                                                                              cTACATTGGGGTTGGCGGCGCGTTGATTATCGGCGTGGTAGCTGGTCTGGCGGGCTTGTGGGGCGGTACCATGCTAAAACGCTTGCTGCGGGTGGATGATCTCTGCGATGTCTTCGGTGTGCACGGCGT‑TTGTGGCATTGTCGGCTGTATCATG‑ACCGGGATTTTTGCCGCCCGCTCGCTGGGCGGCGTGGGCTTCGCTGGAGGTGTGACGTTGGGCCATCAGTTGCTGGTACAGCTGGAAAGCATCGccc                                                                                               >  2:62729/1‑250 (MQ=255)                                                                                                                                                    tGGGGTTGGCGGCGCGTTGATTATCGGCGTGGTAGCCGGTCTGGCGGGCTTGTGGGGCGTTACCAAACTCAAAAGCTTGCTGCGGGTGGATGATCTCTGCGATGTCTTCGGTGTGCACGGCGT‑TTGTGGCATTGTTGGCTGTATCATG‑ACCGGGATTTTTGCCGCCAGATCGCTGGGCGGCGTGGGCTTCGCTGAAGGGGTGACGCTGGGCCCTCAGATTCTGGTACAGCTGGCAAGCAACGCCAttccg                                                                                          >  1:143121/1‑247 (MQ=255)                                                                                                                                                                                   gTAGCTGGTCTGGCGGGCTTGTGGGGCGTTACCATGCTCAAACGCTTGCTGCGGGTGGATGATCTCTGCGATGTCTTCGGTGTGCACGGCGT‑TTGTGGCATTGTCGGCTGTATCATG‑ACCGGGATTTTTGCCGCCAGCTCGCTGGGCGGCGTGGGCTTCGCTGAAGGTGTGACGATGGGCCATCAGTTGCTGGTACAGCTGGAAAGCATCGCCATTATGATCGTCTGGTCCGGGGTTGTGGCATTGATCgg                                                          <  1:207336/251‑1 (MQ=255)                                                                                                                                                                                      gctgggctggCGGGCTTGTGGGGCGTTACCATGCTCAAACGCTTGCTGCGGGTGGATGATCTCTGCGATGTCTTCGGTGTGCACGGCGT‑TTGTGGCATTGTCGGCTGTATCATG‑ACCGGGATTTTTGCCGCCAGCTCGCTGGGCGGCGTGGGCTTCGCTGAAGGTGTGACGATGGGCCATCAGTTGCTGGTACAGCTGGAAAGCATCGCCATTACGATCGTCt                                                                                   <  2:84084/223‑1 (MQ=255)                                                                                                                                                                                      gCTGGTCTGGCGGGCTTGTGGGGCGTTACCATGCTCAAACGCTTGCTGCGGGTGGATGATCTCTGCGATGTCTTCGGTGTGCACGGCGT‑TTGTGGCATTGTCGGCTGTATCATG‑ACCGGGATTTTTGCCGCCAGCTCGCTGGGCGGCGTGGGCTTCGCTGAAGGTGTGACGATGGGCCATCAGTTGCTGGTACAGCTGGAAAGCATCGCCATTACGATCGTCt                                                                                   >  1:84084/1‑223 (MQ=255)                                                                                                                                                                                                             cGTTACCATGCTCAAACGCTTGCTGCGGGTGGATGATCTCTGCGATGTCTTCGGTGTGCACGGCGT‑TTGTGGCATTGTCGGCTGTATCATG‑ACCGGGATTTTTGCCGCCAGCTCGCTGGGCGGCGTGGGCTTCGCTGAAGGTGTGACGATGGGCCATCAGTTGCTGGTACAGCTGGAAAGCATCGCCATTACGATCGTCTGGTCCGGTGTTGTGGCATTTATCGGCTCCAAATTGGCGGATCTGACGGTTg                                >  1:259379/1‑251 (MQ=255)                                                                                                                                                                                                              gTTACCATGCTCAAACGCTTGCTGCGGGTGGATGATCTCTGCGATGTCTTCGGTGTGCACGGCGT‑TTGTGGCATTGTCGGCTGTATCATG‑ACCGGGCTTTTTGCCGCCAGCTCGCTGGGCGGCGTGGGCTTCGCTGAAGGTGTGACGATGGGCCATCAGTTGCTGGTACAGCTGGACAGCATCGCCATTACGATCGTCTGGTCCGGTGTTGTGGCATTTATCGGCTACAAATTGGCGGATCTGACGGTTgg                               >  1:128216/1‑251 (MQ=255)                                                                                                                                                                                                                             cGCTTGCTGCGGGTTGATGATCTCTGCGATGTCGTCGGTGTGCACGGCGT‑TTGTGGCATTGTCGGCTGTATCATG‑ACCGGGATTTTTGCCGCCAGCTCGCTGGGCGGCGTGGGCTTCGCTGAAGGTGTGACGATGGGCCATCAGTTGCTGGTACAGCTGGAAAGCATCGCCATTACGATCGTCTGGTCCGGTGTTGTGGCATTTATCGGCTACAAATGGGCGGATCTGACGGTTGGTCTGCGTGTACCGGa                <  1:220530/251‑1 (MQ=255)                                                                                                                                                                                                                                           ggaggatCTCTGTGATGTCTTCGGTGTGCACGG‑GTGTTGTGGCATTGTCGGCTGTATCATG‑ACCGGGATTTGTGCCGCCAGCTCGCTGGGCGGCGTGGGCTTCGCTGAAGGTGTGACGATGGGCCATCAGTTGCTGGTACAGCTGGAAAGCATCGCCATTACGATCGTCTGGTCCGGTGTTGTGGCATTTATCGGCTACAAATTGGCGGATCTGACGGTTGGTCTGCGTGTACCGGAAGAGCAGGAGCgag  <  2:166/247‑1 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                     ATTTGTGAATACTGTGGTCGCAACGGCGGCGGCAATTCTTGGCTGGATCTTCGGTGAATGGGCGCTGCGTGGTAAGCCTTCACTGCTGGGGGCGTGTTCTGGCGCGATTGCCGGTCTGGTCGGCGTGACGCCAGCCTGCGGCTACATTGGGGTTGGCGGCGCGTTGATTATCGGCGTGGTAGCTGGTCTGGCGGGCTTGTGGGGCGTTACCATGCTCAAACGCTTGCTGCGGGTGGATGATCCCTGCGATGTCTTCGGTGTGCACGGCGT‑TTGTGGCATTGTCGGCTGTATCATG‑ACCGGGATTTTTGCCGCCAGCTCGCTGGGCGGCGTGGGCTTCGCTGAAGGTGTGACGATGGGCCATCAGTTGCTGGTACAGCTGGAAAGCATCGCCATTACGATCGTCTGGTCCGGTGTTGTGGCATTTATCGGCTACAAATTGGCGGATCTGACGGTTGGTCTGCGTGTACCGGAAGAGCAGGAGCGAG  >  NC_000913/473721‑474205

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑