Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	472,159	C→T	D508D (GAC→GAT)	mdlB →	ABC transporter family protein MdlB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	472,159	0	C	T	96.9%	99.6 / NA	32	D508D (GAC→GAT)	mdlB	ABC transporter family protein MdlB
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (15/16);  minor base G (1/0);  total (16/16)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CTGGCGGATACCTTCCTCGCCAACGTGACGCTGGGGCGGGATATCTCCGAAGAACGCGTCTGGCAGGCGCTGGAAACCGTGCAACTGGCGGAGCTGGCGCGTAGCATGAGCGACGGTATTT‑ACACGCCGCTGGGCG‑AGCAGGGGAATAATCTCTCAGTTGGGCAAAAGCAACTGCTGGCACTGGCGCGCGTGCTGGTCGAGACGCCGCAAATCCTGATCCTTGATGAGGCAACCGCCAGCATTGACTCCGGTACTGAACAGGCGATTCAACATGCTCTGGCGGCGGTGCGTGAACATACCACGCTGGTAGTGATTGCTCACCGCTTATCGACCATTGTTGATGCCGACACCATTCTGGTGCTTCATCGTGGGCAAGCCGTGGAGCAGGGCACTCACCAGCAACTGCTGGCGGCCCAGGGACGCTACTGGCAGATGTATCAACTGCAACTTGCGGGCGAAGAGCTGGCAGCCAGC  >  NC_000913/471914‑472387                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                     gctctccggatctcccTCGCCAACGGGACGCTGGGGCGGGATTTCCCCGAAAAACGCGTCGGGCAGGCGCGGGAACCCGTGAAACTGGCGGAGTGGGCGCGTAGCAGGAGCGCCGTTATTT‑ACACGCCGCTGGGCG‑AGCAGGGGAATAATCTCTCAGTTGGGCAAAAGCAACTGCTGGCACTGGCGCGCGTGCTGGTCGAGACGCCGCAAATCCTGATCCTTGATGAGGCAACCGCCAGCATTGATTCCg                                                                                                                                                                                                                                  <  1:185654/236‑1 (MQ=255)       gATACCTTCCTCGCCAACGTGACGCTGGGGCGGGATATCTCCGAAGAACGCGTCTGGCAGGCGCTGGAAACCGTGCAACTGGCGGAGCTGGCGCGTAGCATGAGCGACGGTATTT‑ACACGCCGCTGGGCG‑AGCAGGGGAATAATCTCTCAGTTGGGCAAAAGCAACTGCTGGCACTGGCGCGCGTGCTGGTCGAGACGCCGCAAATCCTGATCCTTGATGAGGCAACCGCCAGCATTGCTTCCGGTACTg                                                                                                                                                                                                                            >  1:115149/1‑250 (MQ=255)          aCCTTCCTCGCCAACGTGACGCTGGGGCGGGATATCTCCGAAGAACGCGTCTGGCAGGCGCTGGAAACCGTGCAACTGGCGGAGCTGGCGCGTAGCATGAGCGACGGTATTT‑ACACGCCGCTGGGCG‑AGCAGGGGAATAATCTCTCAGTTGGGCAAAAACAACTGCTGGCACTGGCGCGCGTGCTGGTCGAGACGCCGCAAATCCTGATCCTTGATGAGGCAACCGCCAGCATTGATTCCGGTACTGAAcc                                                                                                                                                                                                                        >  2:139056/1‑250 (MQ=255)           cctccctcgcaaccgggccgcgggggCGGGATATCCCGGAAGAACGGGTCGGGCAGGCGCTGAAACCCGTGCATCGGGGGGAGTTGGCGCGTAGCATGAGCGACGGTATTT‑CCACGCCGCTGGGCG‑AGCAGGGTAATAATCTCGCAGTTGGGCAAAAGCAACTGCTGGCACTGGTGCGCGTGCTGGTCGAGACGCCGCAAATCCTGATCCTTGAGGAGGCAACCGCCAGCATTGATTCCGGTACTGAACAg                                                                                                                                                                                                                       <  1:215004/229‑1 (MQ=255)              tCCTCGCCAACGTGACGCTGGGGCGGGATATCTCCGAAGAACGCGTCTGGCAGGCGCTGGAAACCGTGCAACTGGCGGAGCTGGCGCGTAGCATGAGCGACGGTATTT‑ACACGCCGCTGGGCG‑AGCAGGGGAATAATCTCTCAGTTGGGCAAAAGCAACTGCTGGCACTGGCGCGCGTGCTGGTCGAGACGCCGCAAATCCTGATCCTTGATGAGGCAACCGCCAGCATTGATTCCg                                                                                                                                                                                                                                  >  2:185654/1‑237 (MQ=255)                          tGACGCTGGGGCGGGATATCTCCGAAGAACGCGTCTGGCAGGCGCTGGAAACCGTGCAACTGGCGGAGCTGGCGCGTAGCATGAGCGACGGTATTT‑ACACGCCGCTGGGCG‑AGCAGGGGAATAATCTCTCAGTTGGGCAAAAGCAACTGCTGGCACTGGCGCGCGTGCTGGTCGAGACGCCGCAAATCCTGATCCTTGATGAGGCAACCGCCAGCATTGGTTCCGGTACTGAACAGGCGATTCAACATGcc                                                                                                                                                                                                        >  2:34556/1‑250 (MQ=255)                            aCGCTGGGGCGGGATATCTCCGAAGAACGCGTCTGGCAGGCGCTGGAAACCGTGCAACTCGCGGAGCCGTCGCCTCGCATGAGCGACGGAATTT‑ACACGCCGCTGGGCG‑AGCAGGGGAATAATCTATCAGGCGGGCAAAAGCAACAGCAGGCACTAGCGCGCGTGCTGGCCGAGACGCCGCAAATCCTGATCCTTGATGAGGCAACCGCAAGCATTGATACCGGTACTGCACAGGCGATTCAACCTGctct                                                                                                                                                                                                      >  1:15684/1‑251 (MQ=255)                              gCTGGGGCGGGATATCTCCGAAGAACGCGTCTGGCAGGCGCTGGAAACCGTGCAACTGGCGGAGCTGGCGCGTAGCATGAGCGACGGTATTT‑ACACGCCGCTGGGCG‑AGCAGGGGAATAATCTCTAAGTTGGGCAAAAGCAACTGCTGGCACTGGCGCGCGTGCTGGTCGAGACGCCGCAAATCCTGATCCTTGATGAGGCAACCGCCAGCATTGATTCCGGTACTGAACAGGCGATTCAACATGCTCTgg                                                                                                                                                                                                    >  1:239149/1‑251 (MQ=255)                                         atatCTCCGAAGAACGCGTCTGGCAGGCGCTGGAAACCGTGCAACTGGCGGAGCTGGCGCGTAGCATGAGCGACGGTATTT‑ACACGCCGCTGGGCG‑AGCAGGGGAATAATCTCTCAGTTGGGCAAAAGCAACTGCTGGCACTGGCGCGCGTGCTGGTCGAGACGCCGCAAATCCTGATCCTTGATGAGGCAACCGCCAGCATTGATTCCGGTACTGAACAGGCGATTCAACATGCTCTGGCGGCGGTGCGt                                                                                                                                                                                         >  1:197195/1‑251 (MQ=255)                                                   agaccgcgTCGGGCGGGCGCGGGAACCGGTGCAACGGGGGGGGCGGGCGCTTAGCATGAGCGACGGTATTT‑ACACGCCGCGGGGCG‑AGCAGGGGAATAATCTCTCAGTTGGGAAAAAGCAACTGCTGGCACTGGCGCGCGTGCTGGTCGAGACGCCGCAAATCCTGATCCTTGAGGAGGCAACCGCCAGCATTGATTCCGGTACTGAACAGGCGATTCAACATGCTCTGGCGGCGGTGCGTGAACATACCa                                                                                                                                                                               <  1:88285/247‑1 (MQ=255)                                                                   gcgcTGGAAACCGTGCAACTGGCGGAGCTGGCGCGTAGCATGAGCGACGGTATTT‑ACACGCCGCTGGGCG‑AGCAGGGGAATAATCTCTCAGTTGGGCAAAAGCAACTGCTGGCACTGGCGCGCGTGCTGGTCGAGACGCCGCAAATCCTGATCCTTGATGAGGCAACCGCCAGCATTGATTCCGGTACTGAACAGGCGATTCAACATGCTCTGGCGGCGGTGCGTGAACATACCACGCTGGTAGTGATTGc                                                                                                                                                               >  1:10543/1‑251 (MQ=255)                                                                               gTGCAACTGGCGGGGGTGGCGCGTAGCATGAGCGACGGTATTT‑ACACGCCGCTGGGCG‑AGAAGGGGAATAATCTCTCAGTTGGGCAAAAGCAAATGCTGGCACTGGCGCGCGTGCTGGTCGAGAAGCCGCAAATCATGATCCTTGATGAGGCAACCGCCAGCATTGAGTCCGGTACTGACCAGGCGCTTCAACATGCTCTGGCGGCGGTGCGTGAACATACCACGCTGGTAGTGAGTGCTCACCGCTTATc                                                                                                                                                   >  2:157232/1‑251 (MQ=255)                                                                               gTGCAACGGGCGGAGTGGGGGGGTAGCATGGGGGGCGGTTTTT‑ACACGCCGTTGGGCG‑AGCAGGGGAATAATCTCTCAGTGGGGAAAAAGCAACTGCTGGCACTGGCGCGCGTGCGGGTCGAGACGCCGCAAATCCTGATCCTTGATGAGGCAACCGCCAGCATTGATTCCGGTACTGAACAGGCGATTCAACATGCTCTGGCGGCGGTGCGTGAACATACCACGCTGGTAGTGATTGCTCACCGCTTATc                                                                                                                                                   <  1:139074/251‑1 (MQ=255)                                                                                       gccGGAGCGGGCTCGTAGATTGAGCGACG‑TATTTTACACGCCGCGGGGCG‑AGCAGGGGAATAATCTCTCAGTTGGGCAAAAGCAACTGCTGGCACTGGCGCGCTTTCTGGTCGAGACGCCGCAAACCCTGATCCTTGATGAGGCAACCGCCAGCATTGATTCCGGTACTGAACAGGCGATTCAACATGCTCTGGCGGCGGTGCGTGAACATACCACGCTGGTAGTGATTGCTCACCGCTTATCGACCAtt                                                                                                                                            <  1:148139/248‑1 (MQ=255)                                                                                                   gggaaGCAGGAGCGAGGGTATTT‑GCACGCCGCGGGGCG‑AGCAGGGGAATAATCGCTCAGTTGGGAAAAAGCAATTGCGGGCACTGGGGCGCGGGCTGGTCGAGACGCCGAAAATCCTGATCCTTGATGAGGCAACCGCCAGCATTGATTCCGGTACTGAACAGGCGATTCAACATGCTCTGGCGGCGGTGCGTGAACATACCACGCTGGTAGTGATTGCTCACCGCTTATCGACCATTGTTGATGCCGaca                                                                                                                               <  1:139056/247‑1 (MQ=255)                                                                                                       agcatgggcggtggTATTT‑ACCCGCCGCGGGGCG‑AGCAGGGGAATAAGCACTCAGTGGGTAAAAAGCACCGGCGGCCCCGGGCGTGCGGGCTGGTCGGGACGCCGCAAATCCTGATCTTTAATGAGGCAACCGCCAGCATTGATTCCGGTACTGAACAGGCGATTCAACATGCTCTGGCGGCGGTGCGTGAACATACCACGCTGGTAGTGATTGCTCACCGCTTATCGACCATTGTTGATGCCGCCACCAt                                                                                                                           <  2:38754/239‑1 (MQ=255)                                                                                                         gatgagacagggTATTT‑CCACGCC‑CTGGGGGGAGCGGGGTAATAATCTCGCAGTTGGGCAAAAGCAACTGCTGCCACGGGCGCGGGTGCTGGTGGAGACGCCGAAACTCCTGATCCTGGATGAGGCAACCGCCAGCATTGATTCCGGTACTGAACAGGCGATTCAACATGCTCTGGCGGCGGTGCGTGAACATACCACGCTGGTAGTGATTGCTCACCGCTTATCGACCATTGTTGATGCCGACACCATTc                                                                                                                         <  2:75943/241‑1 (MQ=255)                                                                                                            gAGCGACGGTATTT‑ACACGCCGCTGGGCG‑AGCAGGGGAATAATCTCTCAGTTGGGCAAAAGCAACTGCTGGCACTGGCGCGCGTGCTGGTCGAGACGCCGCAAATCCTGATCCTTGATGAGGCAACCGCCAGCATTGATTCCGGTACTGAACAGGCGATTCAACATGCTCTGGCGGCGGTGCGTGAACATACCACGCTGGTAGTGATTGCTCACCGCTTATCGACCATTGTTGATGCCGACACCATTCTgg                                                                                                                      >  1:2044/1‑251 (MQ=255)                                                                                                            gAGCGACGGTATTT‑ACACGCCGCTGGGCG‑AGCAGGGGAATAATCTCTCAGTTGGGCAAAAGCAACTGCTGGCACTGGCGCGCGTGCTGGTCGAGACGCCGCAAATCCTGATCCTTGATGAGGCAACCGCCAGCATTGATTCCGGTACTGAACAGGCGATTCAACATGCTCTGGCGGCGGTGCGTGAACATACCACGCTGGTAGTGATTGCTCACCGCTTATCGACCATTGTTGATGCCGACACCATTCTgg                                                                                                                      >  1:20109/1‑251 (MQ=255)                                                                                                                 aatgatttgaacttgcctatGGGCG‑AGGAGGGTAAAAATCCCTCAGTTGGTAAAACGCAACGGTTGACCCTGGTGCGCTTTCTGGTCTAGACTCGGAAAACCCTGATCCTTGATGAGGCACCCGCCAGCATTTATTCCGGTACTGAACAGGCGATTCACCATGCTCTTGCGGCGGTGCGTTAACATTCCACGCTGGTAGTGATTGCTCACCGCTTTTCGGCCATTGTTGATGCCGACACCATTCTGGTGCt                                                                                                                  <  1:60908/232‑1 (MQ=255)                                                                                                                              cgccgcTGGGCG‑AGCAGGGGAATAATCTCTCAGTTGGGCAAAAGCAACTGCTGGCACTGGCGCGCGTGCTGGTCGAGACGCCGCAAATCCTGATCCTTGATGAGGCAACCGCCAGCATTGATTCCGGTACTGAACAGGCGATTCAACATGCTCTGGCGGCGGTGCGTGAACATACCACGCTGGTAGTGATTGCTCACCGCTTATCGACCATTGTTGATGCCGACACCATTCTGGTGCTTCATCGTGGGCa                                                                                                      >  1:115009/1‑250 (MQ=255)                                                                                                                                       gCG‑AGCCGGGGAATAATCTCTCAGTTGGGCAAAAGCAACTGCTGGCACTGGCGCGCGTGCTGGTCGAGACGCCGCAAATCCTGATCCTTGATGAGGCAACCGCCAGCATTGATTCCGGTACTGAACAGGCGATTCAACATGCTCTGGCGGCGGTGCGTGAACATACCACGCTGGTAGTGATTGCTCACCGCTTATCGACCATTGTTGATGCCGACACCATTCTGGTGCTTCATCGTGGGCAAGCCGTgg                                                                                              >  2:230260/1‑249 (MQ=255)                                                                                                                                              aGGGGAATAATCTCTCAGTTGGGCAAAAGCAACTGCTGGCACTGGCGCGCGTGCTGGTCGAGACGCCGCAAATCCTGATCCTTGATGAGGCAACCGCCAGCATTGATTCCGGTACTGAACAGGCGATTCAACATGCTCTGGCGGCGGTGCGTGAACATACCACGCTGGTAGTGATTGCTCACCGCTTATCGACCATTGTTGATGCCGACACCATTCTGGTGCTTCATCGTGGGCAAGCCGTGGAGCAGGGc                                                                                      >  1:214455/1‑251 (MQ=255)                                                                                                                                                        tctctcAGTTGGGCAAAAGCAACTGCTGCCTCTGGCGCGCGTGCGGGTCGAGACGCCGCAAACCCTGTTCCTTGATGAGGCAACCGCCAGCATTGATTCCGGTACTGAACAGGCGATTCAACATGCTCTGGCGGCGGTGCGTGAACATACCACGCTGGTAGTGATTGCTCACCGCTTATCGACCATTGTTGATGCCGACACCATTCTGGTGCTTCATCGTGGGCAAGCCGTGGAGCAGGGCACTCACCAg                                                                             <  2:115149/250‑1 (MQ=255)                                                                                                                                                              aGTTGGGCAAAAGCAACTGCTGGCCCTGGCGCGCGTGCTGGTCGAGACGCCGCAACTCCTGATCCTTGATGAGGCAACCGCCAGCCTTGATTCCGGTACTGAACAGGCGATTCAACATGCTCTGGCGGCGGTGCGTGAACATACCACGCTGATAGGGATTACTCACCGCTTATCGTCCATTGTTGATGCCGACACCATTCTGGTGCTTCATCCTGGGCACGCCGTAGTGCAGGGCACTCACCCGCCActgc                                                                      >  2:188665/1‑251 (MQ=255)                                                                                                                                                               gtggggaaaaaGCAACTGCTGGCACGGGCGCGCGTGCTGGTCGAGACGCCGCAAATCCTGATCCTTGATGAGGCAACCGCCAGCATTGATTCCGGTACTGAACAGGCGATTCAACATGCTCTGGCGGCGGTGCGTGAACATACCACGCTGGTAGTGATTGCTCACCGCTTATCGACCATTGTTGATGCCGACACCATTCTGGTGCTTCATCGTGGGCAAGCCGTGGAGCAGGGCACTCACCAGCAActgct                                                                     <  1:34556/244‑1 (MQ=255)                                                                                                                                                                                   ggCACTGGCGCGCGTGCTGGCCGAGCCGCCGAAAATCCTGATCCTTGATGAGGCAACCGCCAGCATTGATTCCGGTACTGAACAGGCGATTCAACATGCTCTGGCGGCGGTGCGTGAACATACCACGCTGGTAGTGATTGCTCACCGCTTATCGACCATTGTTGATGCCGACACCATTCTGGTGCTTCATCGTGGGCAAGCCGTGGAGCAGGGCACTCACCAGCAACTGCTGGCGGCCCAGGGACGCTACt                                                 <  2:116898/251‑1 (MQ=255)                                                                                                                                                                                              gcgTGCTGGTCGAGACGCCGCAAATCCTGATCCTTGATGAGGCAACCGCCAGCATTGATTCCGGTACTGAACAGGCGATTCAACATGCTCTGGCGGCGGTGCGTGAACATACCACGCTGGTAGTGATTGCTCACCGCTTATCGACCATTGTTGATGCCGACACCATTCTGGTGCTTCATCGTGGGCAAGCCGTGGAGCAGGGCACTCACCAGCAACTGCTGGCGGCCCAGGGACGCTACTGGCAGATGTAt                                      <  1:57692/251‑1 (MQ=255)                                                                                                                                                                                                            aCGCCGCAAACCGTGTCCCTTGATGAGGAAGCCGCCAGCATTGATTCCGGTACTGAACAGGCGATTCAACAGGCGCGGGCGGCGGTGCGTGAACATACCACGCTGGTAGTGATTTCTCGCCGCTTATCGACCATTGTTGATGCCGACTCCATTCTGGTGCTGCATCGGGGGCAAGCCGTGGAGCAGGGCACTCACCAGCAACGGCTGGCGGGCCAGGGACGCTACTGGCAGATGTATCAACTGCAACtggc                        <  2:27671/251‑4 (MQ=255)                                                                                                                                                                                                                ggcaattcttgagccTTGATGGGGAAACCGCCAGCATTGATTCCGGTACTGAACAGGCGATTCAACATGCTCTGGCGGCGGTGCGTGAACATACCACGCTGGTAGTGATTGCTCACCGCTTATCGACCATTGTTGATGCCGACACCATTCTGGTGCTTCATCGTGGGCAAGCCGTGGAGCAGGGCACTCACCAGCAACTGCTGGCGGCCCAGGGACGCTACTGGCAGATGTATCAACTGCAACTTGCGGGc                    <  1:72580/238‑1 (MQ=255)                                                                                                                                                                                                                 gcAAATCCTGATCCTTGATGAGGCAACCGCCAGCATTGATTCCGGTACTGAAAAGGCGATTCAACATGCTCTGGCGGCGGTGCGTGAACATACCACGCTGGTAGTGATTGCTCACCGCTTATCGACCATTGTTGATGCCGACACCATTCTGGTGCTTCATCGTGGGCAAGCCGTGGAGCAGGGCACTCACCAGCAACTGCTGGCGGCCCAGGGACGCTACTGGCAGATGTATCAACTGCAACTTGCGGGCg                   >  1:217139/1‑251 (MQ=255)                                                                                                                                                                                                                                  gtggggCAACCGCCAGCATTGATTCCGGTACTGAACAGGCGATTCAACATGCTCTGGCGGCGGTGCGTGAACATACCACGCTGGTAGTGATTGCTCACCGCTTATCGACCATTGTTGATGCCGACACCATTCTGGTGCTTCATCGTGGGCAAGCCGTGGAGCAGGGCACTCACCAGCAACTGCTGTCGGCCCAGGGACGCTACTGGCAGATGTATCAACTGCAACTTGCGGTCGAAGAGCTGGcagccagc  <  2:37133/247‑1 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                     CTGGCGGATACCTTCCTCGCCAACGTGACGCTGGGGCGGGATATCTCCGAAGAACGCGTCTGGCAGGCGCTGGAAACCGTGCAACTGGCGGAGCTGGCGCGTAGCATGAGCGACGGTATTT‑ACACGCCGCTGGGCG‑AGCAGGGGAATAATCTCTCAGTTGGGCAAAAGCAACTGCTGGCACTGGCGCGCGTGCTGGTCGAGACGCCGCAAATCCTGATCCTTGATGAGGCAACCGCCAGCATTGACTCCGGTACTGAACAGGCGATTCAACATGCTCTGGCGGCGGTGCGTGAACATACCACGCTGGTAGTGATTGCTCACCGCTTATCGACCATTGTTGATGCCGACACCATTCTGGTGCTTCATCGTGGGCAAGCCGTGGAGCAGGGCACTCACCAGCAACTGCTGGCGGCCCAGGGACGCTACTGGCAGATGTATCAACTGCAACTTGCGGGCGAAGAGCTGGCAGCCAGC  >  NC_000913/471914‑472387

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑