Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	465,894	C→T	E473E (GAG→GAA)	ybaE ←	uncharacterized protein YbaE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	465,894	0	C	T	100.0%	32.2 / NA	11	E473E (GAG→GAA)	ybaE	uncharacterized protein YbaE
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (8/3);  total (8/3)

CCGTTCACGCCGGGAGGGGCACTAATGCGATAGTGATAGTTGAACAGCGGCGTCAGCGTCGCATCTGTCATTAACTGGCTAAAAACCGCTTTCAGGGCATTAAATCGGTTTTCTTCATCAGGCATTATTTGCACGGCATCCAGTGTCGATTGTAGATGTGCGTATGCTGGAGCGTCGAAAACATGTGGCCACAGCGGATCGCAGCGCAGCCATTGCTCCAGAGTATATTCCGGTGCTTCGCCAATTAATCTGTCGCCCATCATGAGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCGTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGG  >  NC_000913/465679‑466118                                                                                                                                                                                                                        |                                                                                                                                                                                                                                 ccGTTCACGCCGGGAGGGGCACTAATGCGATAGTGATAGTTGAACAGCGGCGTCAGCGTCGCATCTGTCATTAACTGGCTAAAAACCGCTTTCAGGGCATTAAATCGGTTTTCTTCATCAGGCATTATTTGCACGGCATCCAGTGTCGATTGTAGATGTGCGTATGCTGGAGCGTCGAAAACATGTGGCCACAGCGGATCGCAGCGCAGCCATTGTTCCAGAGTATATTCCGGTGCTTCGCCAATTAATCt                                                                                                                                                                                               <  1:228692/251‑1 (MQ=255)            gggAGGGGCACTAATGCGATAGTGATAGTTGAACAGCGGCGTCAGCGTCGCATCTGTCATTAACTGGCTAAAAACCGCTTTCAGGGCATTAAATCGGTTTTCTTCATCAGGCATTATTTGCACGGCATCCAGTGTCGATTGTAGATGTGCGTATGCTGGAGCGTCGAAAACATGTGGCCACAGCGGATCGCAGCGCAGCCATTGTTCCAGAGTATATTCCGGTGCTTCGCCAATTAATCTGTCGCCcatc                                                                                                                                                                                     >  1:248219/1‑250 (MQ=255)                 gggCACTAATGCGATAGTGATAGTTGAACAGCGGCGTCAGCGTCGCATCTGTCATTAACTGGCTAAAAACCGCTTTCAGGGCATTAAATCGGTTTTCTTCATCAGGCATTATTTGCACGGCATCCAGTGTCGATTGTAGATGTGCGTATGCTGGAGCGTCGAAAACATGTGGCCACAGCGGATCGCAGCGCAGCCATTGTTCCAGAGTATATTCCGGTGCTTCGCCAATTAATCTGTCGCCCTTCATgtgg                                                                                                                                                                               >  2:141299/1‑248 (MQ=255)                            cgctagtgatagtTGGACAGCGGCGCCAGCGTAGCATCTGGCATTAACTGGCTAAAAAACGCTTTCAGGGCATTAAATCGGTTTTCTTCATCAGGCAATATTTGCACGGCATCCAGTGTCGATTGTAGATGTGCGTATGATGGTGCGTCGAAAACGTGTGTCCACAGCGGATCGCAGTGCAGCCATTGTTCCAGAGTATATTCCGGTGCTTCGCCAATTAATCTGTAGCCCATCATGCGGTCTGAGTGTGc                                                                                                                                                                    >  2:54031/4‑251 (MQ=255)                              atagtgatagtTGAACAGCAGCGTCAGCGTCGCATCTGTCATTAACTGGCTAAAAACCGCTTTCAGGGCATTAAATCGGTTTTCTTCATCAGGCATTATTTGCACGGCATCCAGTGTCGATTGTAGATGTGCGTATGCTGGAGCGTCGAAAACATGTGGCCACAGCGGATCGCAGCGCAGCCATTGTTCCAGAGTATATTCCGGTGCTTCGCCAATTAATCTGTCGCCCATCATGAGGTCTGCGTGTGCCt                                                                                                                                                                  >  1:48850/1‑251 (MQ=255)                                                              cATCTGTCATTAACTGGCTAAAAGCCGCTTTCATGGCATTAAATCGGTTTTCTTCATCAGGCTTTATTTGCACGGCATCCAGTGGCGATTGTAGATGTGCGTATGCTGGAGCGGCGAAAACATGTGGCCACATCGGATCGCGGCGCAGCCATTGTACCAGAGTATATTCCGGTGCTTCGCCAATTAATCTGTCGCCCATCATGAGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTa                                                                                                                                  <  1:241881/251‑1 (MQ=255)                                                                   gTCATTAACTGGCTAAAAACCGCTTTCAGGGCATTAAATCGGTTTTCTTCATCAGGCATTATTTGCACGGCATCCAGTGGCGATTGTAGATGTGCGTATGCTGGAGCGTCGAAAACATGTGGCCACAGCGGATCGCAGCGCAGCCATTGTTCCAGAGTATATTCCGGTGCTTCGCCAATTAATCTGTCGCCCATCATGAGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATg                                                                                                                                <  1:62811/248‑1 (MQ=255)                                                                                                ggCATTAACTCGGTTTTCTTCATCAGGCATTATTTGCACGGCATCCCGTGTCGATTGTAGATGTGCGTATGCTGAAGCGTCGAAAACATGTGGCCACAGCGGATCGCAGCGCAGCCATTGTTCCAGAGTATATTCCGGTGCTTCGCCAATTAATCTGTCCCCCATCATGAGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTg                                                                                                >  1:95503/1‑251 (MQ=255)                                                                                                                                       ggCATCCAGTGTCGATTGTAGATGTGCGTATGCTGGAGCGTCGAAAACATGTGGCCACAGCGGATCGCAGCGCAGCCATTGTTCCAGAGTATATTCCGGTGCTTCGCCAATTAATCTGTCGCCCATCATGAGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCGTTCTGCCATGGTATGAAGtt                                                         >  2:5798/1‑251 (MQ=255)                                                                                                                                                  tCGATTGTAGATGTGCGTATGCTGGAGCGTCGAAAACATGTGGCCACAGCGGATCGCAGCGCAGCCATTGTTCCAGAGAATATTCCGGTGCTTCGCCAATTAATCTGTCGCCCATCATGAGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTTCCAGTGTCGCCTGTAGGCGTTCTGCCATGGTATGAAGTTCTATCGGTAgg                                              >  1:33724/1‑251 (MQ=255)                                                                                                                                                                                              cacaGCGAATCGCCGCGCAGCCATAGTTCCAGCGTATCTTCCGCTGCTACGCCAATTAATCTGCAGCCCATCATGAGGTCTGCGTGTACCTGTAGGGTCGCGTCGTCCCAGTTTTTTGCGTTATGAAAAATCATTGTGAGTTCACAGCCTTCCGCTGCCCGTGTCGCATGTAGGCGTTCTGCCATGGTCTGAAGTTCTATCGGTCGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCgg  >  2:263284/1‑251 (MQ=255)                                                                                                                                                                                                                        |                                                                                                                                                                                                                                 CCGTTCACGCCGGGAGGGGCACTAATGCGATAGTGATAGTTGAACAGCGGCGTCAGCGTCGCATCTGTCATTAACTGGCTAAAAACCGCTTTCAGGGCATTAAATCGGTTTTCTTCATCAGGCATTATTTGCACGGCATCCAGTGTCGATTGTAGATGTGCGTATGCTGGAGCGTCGAAAACATGTGGCCACAGCGGATCGCAGCGCAGCCATTGCTCCAGAGTATATTCCGGTGCTTCGCCAATTAATCTGTCGCCCATCATGAGGTCTGCGTGTGCCTGTAGGGTCGTGTCGTCCCAGTTTTTTGCGTTATGAAAAATAATTGTGAGTTCACAGCCTTCCGCTGCCAGTGTCGCCTGTAGGCGTTCTGCCATGGTATGAAGTTCTATCGGTAGGTGATAAACCAGCGTCAAGGTTTTCGGTAGTTTGACTTCATCCGG  >  NC_000913/465679‑466118

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑