Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	431,730	C→T	G95D (GGT→GAT)	tsx ←	nucleoside‑specific channel‑forming protein Tsx

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	431,730	0	C	T	100.0%	62.3 / NA	22	G95D (GGT→GAT)	tsx	nucleoside‑specific channel‑forming protein Tsx
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (5/17);  total (5/17)

AGTTCTGCCACTGGTATTTCGCATAGACGTTCATGGACAGGCTCATCGGCAGGCCAGTGTCGATATCGGTACCCAGACCCATGTACCAGGTGCTCTGGCGACCATCTTTATTACGACCCATGTCGTAAATGTAGTTGTTCGCGAAGTACCACTCTTTGAACGGACCGAAGCTAAGGTCAGTATTGGTCAGCTTGTCGATGGAGAAACGTGGTTCGATTTCCATAAACAGCGGAGAACCGTGGTTCCAGATACCTTTAGCATCGGAGTTACCGCCGAAGAATACCGGCGCATCCGCATAACCATAGAAGTCGAACCAGTCTTTTTTAGCGAATGCTTCGTACTCAAGGTAGGTATCGTTGCGGATCTGCGGTCCGAAACGGGTGTGATAGCTTCCGACAACGTTAACGCTCTGGTGCCACCAGTCGGAAAGATACTGCGGTTTGTCGTTTTCAGCTGCGTTGACAGTAAAAGACGAA  >  NC_000913/431494‑431969                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                aGTTCTGCCACTGGTATTTCGCATAGACGTTCATGGACAGGCTCATCGGCAGGCCAGTGTCGATATCGGTACCCAGACCCATGTACCAGGTGCTCTGGCGACCATCTTTATTACGACCCATGTCGTAAATGTAGTTGTTCGCGAAGTACCACTCTTTGAACGGACCGAAGCTAAGGTCAGTATTGGTCAGCTTGTCGATGGAGAAACGTGGTTCGATTTCCATAAACAGCGGAGAATCGTGGTTCCAGATa                                                                                                                                                                                                                                   <  1:15312/251‑1 (MQ=255)      tGCCAATGGTATTTCGCATAGACGTTCATGGACAGGCTCATCGGCAGGCCAGTGTCGATATCGGTACCCAGACCCATGTACCAGGTGCTCTGGCGACCATCTTTATTACGACCCATGTCGAAAATGTAGTTGTTCGCGAAGTACCACTCTTTGAACGGACCGAAGCTAAGGTCAGTATTGGTCAGCTTGTCGATGGTGAAACGTGGTTCGATTTCCATAAAAAGAGGAGAATCGTGGTTCCAGATACCttt                                                                                                                                                                                                                              >  2:249368/1‑251 (MQ=255)                ttttCGCATAGACGTTCATGGACAGGCTCATCGGCAGACCAGTGTCGATATCGGTACCCAGAACCATGTACCAGGTGCTCTGGCGCCCATCTTTATTACGACCCATGTCGTAAATGTAGTTGTTCGCGAAGTACCACTCTTTGAACGGCCCGAAGCTAAGGTCAGTATTGGTCCGCTTGTCGATGGAGAAACGAGGTTCGATTTCCATAAACAGCGGAGAATCGTGGTTCAAGATACCTTTCGAATCgg                                                                                                                                                                                                                      >  2:147773/2‑249 (MQ=255)                                 aTGGACAGGCTCATCGGCAGGCCAGTGTCGATATCGGTACCCAGACCCATGTACCAGGTGCTCTGGCGACCATCTTTATTACGACCCATGTCGTAAATGTAGTTGTTCGCGAAGTACCACTCTTTGAACGGACCGAAGCTAAGGTCAGTATTGGTCAGCTTGTCGATGGAGAAACGTGGTTCGATTTCCATAAACAGCGGAGAATCGTGGTTCCAGATACCTTTAGCATCGGAGTTACCGCCGAAGAATAc                                                                                                                                                                                                   >  2:191173/1‑251 (MQ=255)                                     aCAGGCTCATCGGCAGGCCAGTGTCGATATCGGTTCCCAGACCCATGTCCCAGGGGCTCTGTCGACCATCTTTATTACGACCCATGTCGTAAATGTAGTTGTTCGCGAAGTACCACTCTTGGAACGGACCGAAGGTAAGGTCAGTATTGGTCAGCTTGTCGATGGAGAAACGTGGTTCGATTTCCATAAACAGCGGAGAATCGTGGTTCCAGATACCGTTAGCATCGGAGTTACCGCCGAAGAATACCGgc                                                                                                                                                                                               <  2:247184/251‑1 (MQ=255)                                                                            gACCCATGTACCAGGTGCTCTGGCGACCATCTTTATTACGACCCATGTCGTAAATGTAGTTGTTCGCGAAGTACCACTCTTTGAACGGACCGAAGCTAAGGTCAGTATTGGTCAGCTTGTCGATGGAGAAACGTGGTTCGATTTCCATAAACAGCGGAGAATCGTGGTTCCAGATACCTTTAGCATCGGAGTTACCGCCGAAGAATACCGGCGCATCCGCATAACCATAGAAGTCGAACCAGTCTTTTTTa                                                                                                                                                        <  2:211029/251‑1 (MQ=255)                                                                                   ggaCCAGGTGCTCTGGCGACTATCTTTATTACGACCCATGTCGTAAATGTAGTTGTTCGCGAAGTACCACTCATTGAACGGACCGAAGCTAAGGTCAGTATTGGTCAGCTTGTCGATGGAGAAACGTGGTTCGATTTCCATAAACAGCGGAGAATCGTGGTTCCAGATACCTTTAGCATCGGAGTTACCGCCGAAGAATACCGGCGCATCCGCATAACCATAGAAGTCGAACCAGTCTTTTTTAGCGAAt                                                                                                                                                  <  2:145022/248‑1 (MQ=255)                                                                                           tGCTCTGGCGACCATCTTTATTACGACCCATGTCGTAAATGTAGTTGTTCGCGAAGTACCACTCTTTGAACGGACAGAAGCTCAGGTCAGTATTGGTCAGCTTGTCGATGGAGAAACGTGGTTCGATTTCCATAAACAGCGGAGAATCGTGGTTCCAGATACCTTTAGCATCGGAGTTACCGCCGAAGAATACCGGCGCATCAGCATAACCATAGAAGTCGAACCAGTCTTTTTTAGCGAATGCTTCGTAc                                                                                                                                         >  1:239983/1‑251 (MQ=255)                                                                                            gCTCTGGCGACCATCTTTATTACGACCCATGTCGTAAATGTAGTTGTTCGCGAAGTACCACTCTTTGAACGGACCGAAGCTAAGGTCAGTATTGGTCAGCTTGTCGATGGAGAAACGTGGTTCGATTTCCATAAACAGCGGAGAATCGTGGTTCCAGATACCTTTAGCATCGGAGTTACCGCCGAAGAATACCGGCGCATCCGCATAACCATAGAAGTCGAACCAGTCTTTTTTAGCGAATGCTTCGTAc                                                                                                                                         <  1:171506/250‑1 (MQ=255)                                                                                                     aCCATCGTTATTACGACCCATGTCGTAAATGTAGTTGTTCGCGAAGTACCACTCTTTGAACGGACCGAAGCTAAGGTCAGTATTGGTCAGCTTGTCGATGGAGAAACGTGGTTCGATTTCCATAAACAGCGGAGAATCGTGGTTCCAGATACCTTTAGCATCGGAGTTACCGCCGAAGAATACCGGCGCATCCGCATAACCATAGAAGTCGAACCAGTCTTTTTTAGCGAATGCTTCGTACTCAaggtagg                                                                                                                               <  1:226084/251‑1 (MQ=255)                                                                                                                   gtcccATGTCGTAAATGTAGTTGTTCGCGAAGTACCACTCTTTGAACGGACCGAAGCTAAGGTCAGTATTGGTCAGCTTGTCGATGGAGAAACGTGGTTCGATTTCCATAAACAGCGGAGAATCGTGGTTCCAGATACCTTTAGCATCGGAGTTACCGCCGAAGAATACCGGCGCATCCGCATAACCATAGAAGTCGAACCAGTCTTTTTTAGCGAATGCTTCGTACTCAAGGTAGGTATCGTTGCGGATc                                                                                                                 <  2:59002/249‑1 (MQ=255)                                                                                                                            cGTAAATGTAGTTGTTCGCGAAGTACCACTCTTTGAACGGACCGAAGCTAAGGTCAGTATTGGTCAGCTTGTCGATGGAGAAACGTGGTTCGATTTCCATAAACAGCGGAGAATCGTGGTTCCAGATACCTTTAGCATCGGAGTTACCGCCGAAGAATACCGGCGCATCCGCATAACCATAGAAGTCGAACCAGTCTTTTTTAGCGAATGCTTCGTACTCAAGGTAGGTATCGTTGCGGATCTGCGGTcc                                                                                                         <  2:238787/250‑1 (MQ=255)                                                                                                                                     aGTTGTTCGCGAAGTACCACTCTTTGAACGGACCGAAGCTAAGGTCAGTATTGGTCAGCTTGTCGATGGAGAAACGTGGTTCGATTTCCATAAACAGCGGAGAATCGTGGTTCCAGATACCTTTAGCATCGGAGTTACCGCCGAAGAATACCGGCGCATCCGCATAACCATAGAAGTCGAACCAGTCTTTTTTAGCGAATGCTTCGTACTCAAGGTAGGTATCGTTGCGGATCTGCGGTCCGAAACGGgtg                                                                                               <  1:196294/251‑1 (MQ=255)                                                                                                                                                                aCGGACCGAAGCTAAGGTCAGTATTGGTCAGCTTGTCGATGGAGAAACGTGGTTCGATTTCCATAAACAGCGGAGAATCGTGGTTCCAGATACCTTTAGCATCGGAGTTACCGCCGAAGAATACCGGCGCATCCGCATAACCATAGAAGTCGAACCAGTCTTTTTTAGCGAATGCTTCGTACTCAAGGTAGGTATCGTTGCGGATCTGCGGTCCGAAACGGGTGTGATAGCTTCCGACAACGTTAACGctc                                                                    <  2:260133/251‑1 (MQ=255)                                                                                                                                                                   gACCGAAGCTAAGGTCAGTATTGGTCAGCTTGTCGATGGAGAAACGTGGTTCGATTTCCATAAACAGCGGAGAATCGTGGTTCCAGATACCTTTAGCATCGGAGTTACCGCCGAAGAATACCGGCGCATCCGCATAACCATAGAAGTCGAACCAGTCTTTTTTAGCGAATGCTTCGTACTCAAGGTAGGTATCGTTGCGGATCTGCGGTCCGAAACGGGTGTGATAGCTTCCGACAACGTTAACGCTCTgg                                                                 <  1:191173/251‑1 (MQ=255)                                                                                                                                                                                      aTTGGTCAGCTTGTCGATGGAGAAACGTGGTTCGATTTCCATAAACAGCGGAGAATCGTGGTTCCAGATCCCTTTAGCATCGGAGTTACCGCCGAAGAATACCGGCGCATCCGCATAACCATAGAAGTCGAACCAGTCTTTTTTAGCGAATGCTTCGTACTCAAGGTAGGTATCGTTGCGGATCTGCGGTCCGAAACGGGTGTGATAGCTTCCGACAACGTTAACGCTCTGGTGCCACCAGTCGGAAAgct                                              <  2:66113/251‑3 (MQ=255)                                                                                                                                                                                        tGGTCAGCTTGTCGATGGAGAAACGTGGTTCGATTTCCATAAACAGCGGAGAATCGTGGTTCCAGATACCTTTAGCATCGGAGTTACCGCCGAAGAATACCGGCGCATCCGCATAACCATAGAAGTCGAACCAGTCTTTTTTAGCGAATGCTTCGTACTCAAGGTAGGTATCGTTGCGGATCTGCGGTCCGAAACGGGTGTGATAGCTTCCGACAACGTTAACGCTCTGGTGCCACCAGTCGGAAAGATAc                                            >  1:218560/1‑251 (MQ=255)                                                                                                                                                                                                 tGTCGATGGAGAAACGTGGTTCGATTTCCATAAACAGCGGAGAATCGTGGTTCCAGATACCTTTAGCATCGGAGTTACCGCCGAAGAATACCGGCGCATCCGCATAACCATAGAAGTCGAACTAGTCTTTTTTAGCGAATGCTTCGTACTCAAGGTAGGTATCGTTGCGGATCTGCGGTCCGAAACGGGTGTGATAGCTTCCGACAACGTTAACGCTCTGGTGCCACCAGTCGGAAAGATACTGCGGtt                                     <  1:155396/249‑1 (MQ=255)                                                                                                                                                                                                 ggTCGATGGAGAAACGTGGTTCGATTTCCAGAAACAGCGGAGAATCGTTGTTCCAGATTCCTTTAGCATCGGAGTTACCGCCGAAGAATACCGGCGCAGCCGCATAACCATAGAAGTCGAACCAGTCTTTTTTAGCGAATGCTTCGTACTCAAGGTAGGTATCGTTGCGGATCTGCGGTCCGAAACGGGTGTGATAGCGGCCGGCAATGTGAACGCACTGGTGCGACCAGTCGGAAAGATACTGCGGTTTg                                   <  2:27534/250‑1 (MQ=255)                                                                                                                                                                                                  gTCGATGGAGAAACGTGGTTCGATTTCCATAAACAGCGGAGAATCGTGGTTCCAGATACCTTTAGCATCGGAGTTACCGCCGAAGAATACCGGCGCATCCGCATAACCATAGAAGTCGAACCAGGCTTTTTTAGCGAATGCTTCGTACTCAAGGTAGGTATCGTTGCGGATCTGCGGTCCGAAACGGGTGTGATAGCTTCCGACAACGTTAACGCTCTGGTGCCACCAGTCGGAAAGATACTGCGGTTTGt                                  <  1:37323/251‑1 (MQ=255)                                                                                                                                                                                                                       gATTTCTATAAACATCGGAGAATCGTGGTTCCAGATACCTTTATCATCGGAGTTACCGCCGAAGAATACCGGCGCATCCGCATAACCATAGAAGTCGAACCAGGCTTTTTTAGCGAATGCTTCGTACTCAAGGTAGGTATCGTGGCGGTTCTGCGGTCCGAAACGGGTGTGATAGCTTCCGACAACGTTAACGCTCTGGTGCCTCCAGTCGGAAAGATACTGCGGTCTGTAGTTTTCATCTGCGTTGACAg             <  2:22330/251‑1 (MQ=255)                                                                                                                                                                                                                                  aCAGCGGAGAATCGTGGTTCCAGATACCTTTAGCATCGGAGTTACCGCCGAAGAATACCGGCGCATCCGCATAACCATAGAAGTCGAACCAGTCTTTTTTAGCGAATGCTTCGTACTCAAGGTAGGTATCGTTGCGGATCTGCGGTCCGAAACGGGTGTGATAGCTTCCGACAACGTTAACGCTCTGGTGCCACCAGTCGGAAAGATACTGCGGTTTGTCGTTTTCAGCTGCGTTGACAGTAAAAGACGaa  <  1:188593/251‑1 (MQ=255)                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                AGTTCTGCCACTGGTATTTCGCATAGACGTTCATGGACAGGCTCATCGGCAGGCCAGTGTCGATATCGGTACCCAGACCCATGTACCAGGTGCTCTGGCGACCATCTTTATTACGACCCATGTCGTAAATGTAGTTGTTCGCGAAGTACCACTCTTTGAACGGACCGAAGCTAAGGTCAGTATTGGTCAGCTTGTCGATGGAGAAACGTGGTTCGATTTCCATAAACAGCGGAGAACCGTGGTTCCAGATACCTTTAGCATCGGAGTTACCGCCGAAGAATACCGGCGCATCCGCATAACCATAGAAGTCGAACCAGTCTTTTTTAGCGAATGCTTCGTACTCAAGGTAGGTATCGTTGCGGATCTGCGGTCCGAAACGGGTGTGATAGCTTCCGACAACGTTAACGCTCTGGTGCCACCAGTCGGAAAGATACTGCGGTTTGTCGTTTTCAGCTGCGTTGACAGTAAAAGACGAA  >  NC_000913/431494‑431969

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑